{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T22:31:42Z","timestamp":1789943502005,"version":"4.0.1"},"reference-count":95,"publisher":"The Company of Biologists","license":[{"start":{"date-parts":[[2019,1,1]],"date-time":"2019-01-01T00:00:00Z","timestamp":1546300800000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"funder":[{"DOI":"10.13039\/100000055","name":"National Institute on Deafness and Other Communication Disorders","doi-asserted-by":"publisher","award":["R01 DC009606"],"award-info":[{"award-number":["R01 DC009606"]}],"id":[{"id":"10.13039\/100000055","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000053","name":"National Eye Institute","doi-asserted-by":"publisher","award":["R01 EY017168"],"award-info":[{"award-number":["R01 EY017168"]}],"id":[{"id":"10.13039\/100000053","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>Bardet-Beidl Syndrome (BBS) manifests from genetic mutations encoding for one or more BBS proteins. BBS4 loss impacts olfactory ciliation and odor detection yet the cellular mechanisms remain unclear. Here, we report that Bbs4\u2212\/\u2212 mice exhibit shorter and fewer olfactory sensory neuron (OSN) cilia despite retaining odorant receptor localization. Within Bbs4\u2212\/\u2212 OSN cilia, we observed asynchronous rates of IFT-A\/B particle movements indicating miscoordination in IFT complex trafficking. Within the OSN dendritic knob, the basal bodies are dynamic with incorporation of ectopically expressed centrin-2 and \u03b3-tubulin occurring after nascent ciliogenesis. Importantly, BBS4 loss results in the reduction of basal body numbers separate from cilia loss. Adenoviral expression of BBS4 restored OSN cilia lengths and was sufficient to re-establish odor detection but failed to rescue ciliary and basal body numbers. Our results yield a model for the plurality of BBS4 functions in OSNs that includes intraciliary and periciliary roles that can explain the loss of cilia and penetrance of ciliopathy phenotypes in olfactory neurons.<\/jats:p>","DOI":"10.1242\/jcs.222331","type":"journal-article","created":{"date-parts":[[2019,1,21]],"date-time":"2019-01-21T14:35:36Z","timestamp":1548081336000},"source":"Crossref","is-referenced-by-count":35,"title":["BBS4 is required for IFT coordination and basal body number in mammalian olfactory cilia."],"prefix":"10.1242","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1625-3988","authenticated-orcid":false,"given":"Cedric R.","family":"Uytingco","sequence":"first","affiliation":[{"name":"Department of Pharmacology and Therapeutics, University of Florida College of Medicine, Gainesville, FL 32610, USA"},{"name":"Center for Smell and Taste, University of Florida College of Medicine, Gainesville, FL 32610, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Corey L.","family":"Williams","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Therapeutics, University of Florida College of Medicine, Gainesville, FL 32610, USA"},{"name":"Center for Smell and Taste, University of Florida College of Medicine, Gainesville, FL 32610, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chao","family":"Xie","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Therapeutics, University of Florida College of Medicine, Gainesville, FL 32610, USA"},{"name":"Center for Smell and Taste, University of Florida College of Medicine, Gainesville, FL 32610, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dana T.","family":"Shively","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Therapeutics, University of Florida College of Medicine, Gainesville, FL 32610, 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