{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T22:18:53Z","timestamp":1790461133534,"version":"4.1.0"},"reference-count":62,"publisher":"The Company of Biologists","issue":"13","license":[{"start":{"date-parts":[[2020,8,4]],"date-time":"2020-08-04T00:00:00Z","timestamp":1596499200000},"content-version":"vor","delay-in-days":400,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"funder":[{"DOI":"10.13039\/100000968","name":"American Heart Association","doi-asserted-by":"publisher","award":["16POST31330004"],"award-info":[{"award-number":["16POST31330004"]}],"id":[{"id":"10.13039\/100000968","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000968","name":"American Heart Association","doi-asserted-by":"publisher","award":["18POST34090005"],"award-info":[{"award-number":["18POST34090005"]}],"id":[{"id":"10.13039\/100000968","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000912","name":"March of Dimes Foundation","doi-asserted-by":"publisher","award":["6-FY18-674"],"award-info":[{"award-number":["6-FY18-674"]}],"id":[{"id":"10.13039\/100000912","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R01-AI132463"],"award-info":[{"award-number":["R01-AI132463"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006975","name":"Indiana Clinical and Translational Sciences Institute","doi-asserted-by":"publisher","award":["UL1 TR001108"],"award-info":[{"award-number":["UL1 TR001108"]}],"id":[{"id":"10.13039\/100006975","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006108","name":"National Center for Advancing Translational Sciences","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100006108","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,7,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Centrins are EF-hand containing proteins ubiquitously found in eukaryotes and are key components of centrioles\/basal bodies as well as certain contractile fibers. We previously identified three centrins in the human parasite Toxoplasma gondii, all of which localized to the centrioles. However, one of them, T. gondii (Tg) Centrin2 (CEN2), is also targeted to structures at the apical and basal ends of the parasite, as well as to annuli at the base of the apical cap of the membrane cortex. The role(s) that CEN2 play in these locations were unknown. Here, we report the functional characterization of CEN2 using a conditional knockdown method that combines transcriptional and protein stability control. The knockdown resulted in an ordered loss of CEN2 from its four compartments, due to differences in incorporation kinetics and structural inheritance over successive generations. This was correlated with a major invasion deficiency at early stages of CEN2 knockdown, and replication defects at later stages. These results indicate that CEN2 is incorporated into multiple cytoskeletal structures to serve distinct functions that are required for parasite survival.<\/jats:p>","DOI":"10.1242\/jcs.228791","type":"journal-article","created":{"date-parts":[[2019,6,10]],"date-time":"2019-06-10T09:29:07Z","timestamp":1560158947000},"source":"Crossref","is-referenced-by-count":38,"title":["Centrin2 from the human parasite\n                    <i>Toxoplasma gondii<\/i>\n                    is required for its invasion and intracellular replication"],"prefix":"10.1242","volume":"132","author":[{"given":"Jacqueline M.","family":"Leung","sequence":"first","affiliation":[{"name":"Indiana University Department of Biology , , Bloomington, IN 47405 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Liu","sequence":"additional","affiliation":[{"name":"Indiana University Department of Biology , , Bloomington, IN 47405 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura A.","family":"Wetzel","sequence":"additional","affiliation":[{"name":"Indiana University Department of Biology , , Bloomington, IN 47405 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9778-7337","authenticated-orcid":false,"given":"Ke","family":"Hu","sequence":"additional","affiliation":[{"name":"Indiana University Department of Biology , , Bloomington, IN 47405 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2019,7,1]]},"reference":[{"key":"2026041419064023800_JCS228791C1","doi-asserted-by":"publisher","first-page":"e17","DOI":"10.1371\/journal.ppat.0010017","article-title":"Identification of the moving junction complex of Toxoplasma gondii: a collaboration between distinct secretory organelles","volume":"1","author":"Alexander","year":"2005","journal-title":"PLoS Pathog."},{"key":"2026041419064023800_JCS228791C2","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1242\/jcs.19.1.203","article-title":"Calcium-binding proteins in a vorticellid contractile organelle","volume":"19","author":"Amos","year":"1975","journal-title":"J. Cell Sci."},{"key":"2026041419064023800_JCS228791C3","doi-asserted-by":"publisher","first-page":"995","DOI":"10.1016\/j.cell.2006.07.025","article-title":"A rapid, reversible, and tunable method to regulate protein function in living cells using synthetic small molecules","volume":"126","author":"Banaszynski","year":"2006","journal-title":"Cell"},{"key":"2026041419064023800_JCS228791C4","doi-asserted-by":"publisher","first-page":"e1001094","DOI":"10.1371\/journal.ppat.1001094","article-title":"A novel family of Toxoplasma IMC proteins displays a hierarchical organization and functions in coordinating parasite division","volume":"6","author":"Beck","year":"2010","journal-title":"PLoS Pathog."},{"key":"2026041419064023800_JCS228791C5","doi-asserted-by":"publisher","first-page":"e1004025","DOI":"10.1371\/journal.ppat.1004025","article-title":"RON5 is critical for organization and function of the Toxoplasma moving junction complex","volume":"10","author":"Beck","year":"2014","journal-title":"PLoS Pathog."},{"key":"2026041419064023800_JCS228791C6","doi-asserted-by":"publisher","first-page":"607","DOI":"10.1128\/MMBR.64.3.607-623.2000","article-title":"Lytic cycle of Toxoplasma gondii","volume":"64","author":"Black","year":"2000","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"2026041419064023800_JCS228791C7","doi-asserted-by":"publisher","first-page":"349","DOI":"10.1016\/j.chom.2016.02.006","article-title":"Phosphatidic acid-mediated signaling regulates microneme secretion in Toxoplasma","volume":"19","author":"Bullen","year":"2016","journal-title":"Cell Host Microbe"},{"key":"2026041419064023800_JCS228791C8","doi-asserted-by":"publisher","first-page":"25","DOI":"10.1016\/S0166-6851(99)00160-7","article-title":"Identification and molecular characterization of GRA8, a novel, proline-rich, dense granule protein of Toxoplasma gondii","volume":"105","author":"Carey","year":"2000","journal-title":"Mol. Biochem. Parasitol."},{"key":"2026041419064023800_JCS228791C9","doi-asserted-by":"publisher","first-page":"7433","DOI":"10.1073\/pnas.0307769101","article-title":"A small-molecule approach to studying invasive mechanisms of Toxoplasma gondii","volume":"101","author":"Carey","year":"2004","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2026041419064023800_JCS228791C10","first-page":"114","article-title":"Sequential protein secretion from three distinct organelles of Toxoplasma gondii accompanies invasion of human fibroblasts","volume":"73","author":"Carruthers","year":"1997","journal-title":"Eur. J. Cell Biol."},{"key":"2026041419064023800_JCS228791C11","doi-asserted-by":"publisher","first-page":"225","DOI":"10.1046\/j.1462-5822.1999.00023.x","article-title":"Secretion of micronemal proteins is associated with Toxoplasma invasion of host cells","volume":"1","author":"Carruthers","year":"1999","journal-title":"Cell. Microbiol."},{"key":"2026041419064023800_JCS228791C12","doi-asserted-by":"publisher","first-page":"14346","DOI":"10.1074\/jbc.275.19.14346","article-title":"The Toxoplasma adhesive protein MIC2 is proteolytically processed at multiple sites by two parasite-derived proteases","volume":"275","author":"Carruthers","year":"2000","journal-title":"J. Biol. Chem."},{"key":"2026041419064023800_JCS228791C13","doi-asserted-by":"publisher","first-page":"453","DOI":"10.1084\/jem.20041672","article-title":"Synergistic role of micronemal proteins in Toxoplasma gondii virulence","volume":"201","author":"C\u00e9r\u00e8de","year":"2005","journal-title":"J. Exp. Med."},{"key":"2026041419064023800_JCS228791C14","doi-asserted-by":"publisher","first-page":"e01846-17","DOI":"10.1128\/mBio.01846-17","article-title":"Ablation of an ovarian tumor family deubiquitinase exposes the underlying regulation governing the plasticity of cell cycle progression in Toxoplasma gondii","volume":"8","author":"Dhara","year":"2017","journal-title":"mBio"},{"key":"2026041419064023800_JCS228791C15","doi-asserted-by":"publisher","first-page":"133","DOI":"10.1111\/j.1550-7408.1990.tb05883.x","article-title":"Effect of extracellular ions on motility and cell entry in Toxoplasma gondii","volume":"37","author":"Endo","year":"1990","journal-title":"J. Protozool."},{"key":"2026041419064023800_JCS228791C16","doi-asserted-by":"publisher","first-page":"417","DOI":"10.1042\/BJ20051393","article-title":"A Toxoplasma gondii phosphoinositide phospholipase C (TgPI-PLC) with high affinity for phosphatidylinositol","volume":"394","author":"Fang","year":"2006","journal-title":"Biochem. J."},{"key":"2026041419064023800_JCS228791C17","doi-asserted-by":"publisher","first-page":"520","DOI":"10.1128\/EC.00357-08","article-title":"Efficient gene replacements in Toxoplasma gondii strains deficient for nonhomologous end joining","volume":"8","author":"Fox","year":"2009","journal-title":"Eukaryot. Cell"},{"key":"2026041419064023800_JCS228791C18","doi-asserted-by":"publisher","first-page":"e1001444","DOI":"10.1371\/journal.pbio.1001444","article-title":"Cell division in Apicomplexan parasites is organized by a homolog of the striated rootlet fiber of algal flagella","volume":"10","author":"Francia","year":"2012","journal-title":"PLoS Biol."},{"key":"2026041419064023800_JCS228791C19","doi-asserted-by":"publisher","first-page":"e1004504","DOI":"10.1371\/journal.ppat.1004504","article-title":"Plasticity between MyoC- and MyoA-glideosomes: an example of functional compensation in Toxoplasma gondii invasion","volume":"10","author":"Frenal","year":"2014","journal-title":"PLoS Pathog."},{"key":"2026041419064023800_JCS228791C20","doi-asserted-by":"publisher","first-page":"383","DOI":"10.1083\/jcb.200311137","article-title":"Identification of the membrane receptor of a class XIV myosin in Toxoplasma gondii","volume":"165","author":"Gaskins","year":"2004","journal-title":"J. Cell Biol."},{"key":"2026041419064023800_JCS228791C21","doi-asserted-by":"publisher","first-page":"32","DOI":"10.12688\/wellcomeopenres.11594.1","article-title":"Parasites lacking the micronemal protein MIC2 are deficient in surface attachment and host cell egress, but remain virulent in vivo","volume":"2","author":"Gras","year":"2017","journal-title":"Wellcome Open Res."},{"key":"2026041419064023800_JCS228791C22","doi-asserted-by":"publisher","first-page":"3132","DOI":"10.1093\/emboj\/20.12.3132","article-title":"Toxoplasma evacuoles: a two-step process of secretion and fusion forms the parasitophorous vacuole","volume":"20","author":"Hakansson","year":"2001","journal-title":"EMBO J."},{"key":"2026041419064023800_JCS228791C23","doi-asserted-by":"publisher","first-page":"e7406","DOI":"10.1371\/journal.pone.0007406","article-title":"TgICMAP1 is a novel microtubule binding protein in Toxoplasma gondii","volume":"4","author":"Heaslip","year":"2009","journal-title":"PLoS ONE"},{"key":"2026041419064023800_JCS228791C24","doi-asserted-by":"publisher","first-page":"e1000754","DOI":"10.1371\/journal.ppat.1000754","article-title":"TgMORN1 is a key organizer for the basal complex of Toxoplasma gondii","volume":"6","author":"Heaslip","year":"2010","journal-title":"PLoS Pathog."},{"key":"2026041419064023800_JCS228791C25","doi-asserted-by":"publisher","first-page":"e1002201","DOI":"10.1371\/journal.ppat.1002201","article-title":"The motility of a human parasite, Toxoplasma gondii, is regulated by a novel lysine methyltransferase","volume":"7","author":"Heaslip","year":"2011","journal-title":"PLoS Pathog."},{"key":"2026041419064023800_JCS228791C26","doi-asserted-by":"publisher","first-page":"1003","DOI":"10.1038\/nmeth1134","article-title":"Rapid control of protein level in the apicomplexan Toxoplasma gondii","volume":"4","author":"Herm-G\u00f6tz","year":"2007","journal-title":"Nat. Methods"},{"key":"2026041419064023800_JCS228791C27","doi-asserted-by":"publisher","first-page":"709","DOI":"10.1111\/jeu.12626","article-title":"Tagging of weakly expressed Toxoplasma gondii calcium-related genes with high-affinity tags","volume":"65","author":"Hortua Triana","year":"2018","journal-title":"J. Eukaryot. Microbiol."},{"key":"2026041419064023800_JCS228791C28","doi-asserted-by":"publisher","first-page":"108","DOI":"10.1371\/journal.ppat.0040010","article-title":"Organizational changes of the daughter basal complex during the parasite replication of Toxoplasma gondii","volume":"4","author":"Hu","year":"2008","journal-title":"PLoS Pathog."},{"key":"2026041419064023800_JCS228791C29","doi-asserted-by":"publisher","first-page":"593","DOI":"10.1091\/mbc.01-06-0309","article-title":"Daughter cell assembly in the protozoan parasite Toxoplasma gondii","volume":"13","author":"Hu","year":"2002","journal-title":"Mol. Biol. Cell"},{"key":"2026041419064023800_JCS228791C30","doi-asserted-by":"publisher","first-page":"e13","DOI":"10.1371\/journal.ppat.0020013","article-title":"Cytoskeletal components of an invasion machine-the apical complex of Toxoplasma gondii","volume":"2","author":"Hu","year":"2006","journal-title":"PLoS Pathog."},{"key":"2026041419064023800_JCS228791C31","doi-asserted-by":"publisher","first-page":"e84","DOI":"10.1371\/journal.ppat.0020084","article-title":"Toxoplasma MIC2 is a major determinant of invasion and virulence","volume":"2","author":"Huynh","year":"2006","journal-title":"PLoS Pathog."},{"key":"2026041419064023800_JCS228791C32","doi-asserted-by":"publisher","first-page":"530","DOI":"10.1128\/EC.00358-08","article-title":"Tagging of endogenous genes in a Toxoplasma gondii strain lacking Ku80","volume":"8","author":"Huynh","year":"2009","journal-title":"Eukaryot. Cell"},{"key":"2026041419064023800_JCS228791C33","doi-asserted-by":"publisher","first-page":"2082","DOI":"10.1093\/emboj\/cdg217","article-title":"Rapid invasion of host cells by Toxoplasma requires secretion of the MIC2-M2AP adhesive protein complex","volume":"22","author":"Huynh","year":"2003","journal-title":"EMBO J."},{"key":"2026041419064023800_JCS228791C34","doi-asserted-by":"publisher","first-page":"530","DOI":"10.1126\/science.1165740","article-title":"Rapid membrane disruption by a perforin-like protein facilitates parasite exit from host cells","volume":"323","author":"Kafsack","year":"2009","journal-title":"Science"},{"key":"2026041419064023800_JCS228791C35","doi-asserted-by":"publisher","first-page":"e1001276","DOI":"10.1371\/journal.ppat.1001276","article-title":"The RON2-AMA1 interaction is a critical step in moving junction-dependent invasion by apicomplexan parasites","volume":"7","author":"Lamarque","year":"2011","journal-title":"PLoS Pathog."},{"key":"2026041419064023800_JCS228791C36","doi-asserted-by":"publisher","first-page":"1823","DOI":"10.1111\/j.1462-5822.2005.00646.x","article-title":"The rhoptry neck protein RON4 re-localizes at the moving junction during Toxoplasma gondii invasion","volume":"7","author":"Lebrun","year":"2005","journal-title":"Cell. Microbiol."},{"key":"2026041419064023800_JCS228791C37","doi-asserted-by":"publisher","first-page":"249","DOI":"10.1016\/0166-6851(91)90092-K","article-title":"Characterization of the protein contents of rhoptries and dense granules of Toxoplasma gondii tachyzoites by subcellular fractionation and monoclonal antibodies","volume":"45","author":"Leriche","year":"1991","journal-title":"Mol. Biochem. Parasitol."},{"key":"2026041419064023800_JCS228791C38","doi-asserted-by":"publisher","first-page":"1361","DOI":"10.1091\/mbc.e17-01-0045","article-title":"Stability and function of a putative microtubule organizing center in the human parasite Toxoplasma gondii","volume":"28","author":"Leung","year":"2017","journal-title":"Mol. Biol. Cell"},{"key":"2026041419064023800_JCS228791C39","doi-asserted-by":"publisher","first-page":"1588","DOI":"10.1128\/EC.00082-13","article-title":"Novel thioredoxin-like proteins are components of a protein complex coating the cortical microtubules of Toxoplasma gondii","volume":"12","author":"Liu","year":"2013","journal-title":"Eukaryot. Cell"},{"key":"2026041419064023800_JCS228791C40","doi-asserted-by":"publisher","first-page":"549","DOI":"10.1091\/mbc.e15-11-0754","article-title":"An ensemble of specifically targeted proteins stabilizes cortical microtubules in the human parasite Toxoplasma gondii","volume":"27","author":"Liu","year":"2016","journal-title":"Mol. Biol. Cell"},{"key":"2026041419064023800_JCS228791C41","doi-asserted-by":"publisher","first-page":"257","DOI":"10.1016\/S0166-6851(01)00289-4","article-title":"Characterization of the subpellicular network, a filamentous membrane skeletal component in the parasite Toxoplasma gondii","volume":"115","author":"Mann","year":"2001","journal-title":"Mol. Biochem. Parasitol."},{"key":"2026041419064023800_JCS228791C42","doi-asserted-by":"publisher","first-page":"E115","DOI":"10.1093\/nar\/29.22.e115","article-title":"Modulation of myosin A expression by a newly established tetracycline repressor-based inducible system in Toxoplasma gondii","volume":"29","author":"Meissner","year":"2001","journal-title":"Nucleic Acids Res."},{"key":"2026041419064023800_JCS228791C43","doi-asserted-by":"publisher","first-page":"837","DOI":"10.1126\/science.1074553","article-title":"Role of Toxoplasma gondii myosin A in powering parasite gliding and host cell invasion","volume":"298","author":"Meissner","year":"2002","journal-title":"Science"},{"key":"2026041419064023800_JCS228791C44","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1007\/978-0-387-78267-6_1","article-title":"Current and emerging approaches to studying invasion in apicomplexan parasites","volume":"47","author":"Mital","year":"2008","journal-title":"Subcell. Biochem."},{"key":"2026041419064023800_JCS228791C45","doi-asserted-by":"publisher","first-page":"4341","DOI":"10.1091\/mbc.e05-04-0281","article-title":"Conditional expression of Toxoplasma gondii apical membrane antigen-1 (TgAMA1) demonstrates that TgAMA1 plays a critical role in host cell invasion","volume":"16","author":"Mital","year":"2005","journal-title":"Mol. Biol. Cell"},{"key":"2026041419064023800_JCS228791C46","doi-asserted-by":"publisher","first-page":"193","DOI":"10.1111\/jmi.12559","article-title":"An icosahedral virus as a fluorescent calibration standard: a method for counting protein molecules in cells by fluorescence microscopy","volume":"267","author":"Murray","year":"2017","journal-title":"J. Microsc."},{"key":"2026041419064023800_JCS228791C47","doi-asserted-by":"publisher","first-page":"1559","DOI":"10.1242\/jcs.021089","article-title":"Organellar dynamics during the cell cycle of Toxoplasma gondii","volume":"121","author":"Nishi","year":"2008","journal-title":"J. Cell Sci."},{"key":"2026041419064023800_JCS228791C48","doi-asserted-by":"publisher","first-page":"7463","DOI":"10.1073\/pnas.1114661109","article-title":"Intramembrane proteolysis of Toxoplasma apical membrane antigen 1 facilitates host-cell invasion but is dispensable for replication","volume":"109","author":"Parussini","year":"2012","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2026041419064023800_JCS228791C49","doi-asserted-by":"publisher","first-page":"27","DOI":"10.1016\/S0091-679X(08)61845-2","article-title":"Molecular tools for genetic dissection of the protozoan parasite Toxoplasma gondii","volume":"45","author":"Roos","year":"1994","journal-title":"Methods Cell Biol."},{"key":"2026041419064023800_JCS228791C50","doi-asserted-by":"publisher","first-page":"39","DOI":"10.1016\/0955-0674(95)80043-3","article-title":"Centrin, centrosomes, and mitotic spindle poles","volume":"7","author":"Salisbury","year":"1995","journal-title":"Curr. Opin. Cell Biol."},{"key":"2026041419064023800_JCS228791C51","doi-asserted-by":"publisher","first-page":"420","DOI":"10.1002\/jcp.21226","article-title":"A mechanistic view on the evolutionary origin for centrin-based control of centriole duplication","volume":"213","author":"Salisbury","year":"2007","journal-title":"J. Cell. Physiol."},{"key":"2026041419064023800_JCS228791C52","doi-asserted-by":"publisher","first-page":"795","DOI":"10.1083\/jcb.124.5.795","article-title":"Centrin plays an essential role in microtubule severing during flagellar excision in Chlamydomonas reinhardtii","volume":"124","author":"Sanders","year":"1994","journal-title":"J. Cell Biol."},{"key":"2026041419064023800_JCS228791C53","doi-asserted-by":"publisher","first-page":"343","DOI":"10.1016\/S0304-3991(79)80044-3","article-title":"Digital image processing: the Semper system","volume":"4","author":"Saxton","year":"1979","journal-title":"Ultramicroscopy"},{"key":"2026041419064023800_JCS228791C54","doi-asserted-by":"publisher","first-page":"676","DOI":"10.1038\/nmeth.2019","article-title":"Fiji: an open-source platform for biological-image analysis","volume":"9","author":"Schindelin","year":"2012","journal-title":"Nat. Methods"},{"key":"2026041419064023800_JCS228791C55","doi-asserted-by":"publisher","first-page":"671","DOI":"10.1038\/nmeth.2089","article-title":"NIH Image to ImageJ: 25 years of image analysis","volume":"9","author":"Schneider","year":"2012","journal-title":"Nat. Methods"},{"key":"2026041419064023800_JCS228791C56","doi-asserted-by":"publisher","first-page":"209","DOI":"10.2307\/3276925","article-title":"The fine structure and reproduction of Toxoplasma gondii","volume":"54","author":"Sheffield","year":"1968","journal-title":"J. Parasitol."},{"key":"2026041419064023800_JCS228791C57","doi-asserted-by":"publisher","first-page":"1423","DOI":"10.1016\/j.cell.2016.08.019","article-title":"A Genome-wide CRISPR screen in Toxoplasma identifies essential apicomplexan genes","volume":"166","author":"Sidik","year":"2016","journal-title":"Cell"},{"key":"2026041419064023800_JCS228791C58","doi-asserted-by":"publisher","first-page":"e1002093","DOI":"10.1371\/journal.pbio.1002093","article-title":"A novel bipartite centrosome coordinates the apicomplexan cell cycle","volume":"13","author":"Suvorova","year":"2015","journal-title":"PLoS Biol."},{"key":"2026041419064023800_JCS228791C59","doi-asserted-by":"publisher","first-page":"463","DOI":"10.1126\/science.1204988","article-title":"Host cell invasion by apicomplexan parasites: insights from the co-structure of AMA1 with a RON2 peptide","volume":"333","author":"Tonkin","year":"2011","journal-title":"Science"},{"key":"2026041419064023800_JCS228791C60","doi-asserted-by":"publisher","first-page":"12352","DOI":"10.1073\/pnas.95.21.12352","article-title":"Nuclear-encoded proteins target to the plastid in Toxoplasma gondii and Plasmodium falciparum","volume":"95","author":"Waller","year":"1998","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2026041419064023800_JCS228791C61","doi-asserted-by":"publisher","first-page":"11","DOI":"10.1016\/S0022-1759(99)00109-X","article-title":"96-Well plates providing high optical resolution for high-throughput, immunofluorescence-based screening of monoclonal antibodies against Toxoplasma gondii","volume":"230","author":"Ward","year":"1999","journal-title":"J. Immunol. Methods"},{"key":"2026041419064023800_JCS228791C62","doi-asserted-by":"publisher","first-page":"1903","DOI":"10.1083\/jcb.101.5.1903","article-title":"A nucleus-basal body connector in Chlamydomonas reinhardtii that may function in basal body localization or segregation","volume":"101","author":"Wright","year":"1985","journal-title":"J. Cell Biol."}],"container-title":["Journal of Cell Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1242\/jcs.228791","content-type":"unspecified","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/journals.biologists.com\/jcs\/article-pdf\/doi\/10.1242\/jcs.228791\/3506323\/jcs228791.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/journals.biologists.com\/jcs\/article-pdf\/doi\/10.1242\/jcs.228791\/2060031\/jcs_228791v1.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/journals.biologists.com\/jcs\/article-pdf\/doi\/10.1242\/jcs.228791\/3506323\/jcs228791.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,14]],"date-time":"2026-04-14T23:06:50Z","timestamp":1776208010000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.biologists.com\/jcs\/article\/132\/13\/jcs228791\/163\/Centrin2-from-the-human-parasite-Toxoplasma-gondii"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,7,1]]},"references-count":62,"journal-issue":{"issue":"13","published-print":{"date-parts":[[2019,7,1]]}},"URL":"https:\/\/doi.org\/10.1242\/jcs.228791","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/491316","asserted-by":"object"}]},"ISSN":["1477-9137","0021-9533"],"issn-type":[{"value":"1477-9137","type":"electronic"},{"value":"0021-9533","type":"print"}],"subject":[],"published":{"date-parts":[[2019,7,1]]},"article-number":"jcs228791"}}