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Here, we pioneered the use of Crispr\/Cas9 genome editing to tag an allele of the \u03b24 subunit of the \u03b16\u03b24 integrin. A tdTomato tag was inserted with a linker at the C-terminus of integrin \u03b24 in mouse mammary epithelial cells. Cells harboring this tagged allele were similar to wild-type cells with respect to integrin \u03b24 surface expression, association with the \u03b16 subunit, adhesion to laminin and consequent signaling. These integrin \u03b24 reporter cells were transformed with YAP (also known as YAP1), which enabled us to obtain novel insight into integrin \u03b24 dynamics in response to a migratory stimulus (scratch wound) by live-cell video microscopy. An increase in integrin \u03b24 expression in cells proximal to the wound edge was evident, and a population of integrin \u03b24-expressing cells that exhibited unusually rapid migration was identified. These findings could shed insight into integrin \u03b24 dynamics during invasion and metastasis. Moreover, these integrin \u03b24 reporter cells should facilitate studies on the contribution of this integrin to mammary gland biology and cancer.<\/jats:p>\n               <jats:p>This article has an associated First Person interview with the first author of the paper.<\/jats:p>","DOI":"10.1242\/jcs.231241","type":"journal-article","created":{"date-parts":[[2019,7,1]],"date-time":"2019-07-01T13:29:00Z","timestamp":1561987740000},"source":"Crossref","is-referenced-by-count":15,"title":["Real-time imaging of integrin \u03b24 dynamics using a reporter cell line generated by Crispr\/Cas9 genome editing"],"prefix":"10.1242","volume":"132","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9210-6114","authenticated-orcid":false,"given":"Ameer L.","family":"Elaimy","sequence":"first","affiliation":[{"name":"University of Massachusetts Medical School 1 Department of Molecular, Cell and Cancer Biology , , 55 Lake Avenue North, Worcester, MA 01605 , USA"},{"name":"University of Massachusetts 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