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The CENP-E motor is recruited to polar unattached kinetochores to facilitate chromosome alignment. The spindle checkpoint protein BubR1 (also known as BUB1B) has been reported as a CENP-E interacting partner, but the extent to which BubR1 contributes to CENP-E localization at kinetochores has remained controversial. Here we define the molecular determinants that specify the interaction between BubR1 and CENP-E. The basic C-terminal helix of BubR1 is necessary but not sufficient for CENP-E interaction, and a minimal key acidic patch on the kinetochore-targeting domain of CENP-E is also essential. We then demonstrate that BubR1 is required for the recruitment of CENP-E to kinetochores to facilitate chromosome alignment. This BubR1\u2013CENP-E axis is critical for alignment of chromosomes that have failed to congress through other pathways and recapitulates the major known function of CENP-E. Overall, our studies define the molecular basis and the function for CENP-E recruitment to BubR1 at kinetochores during mammalian mitosis.<\/jats:p>\n                  <jats:p>This article has an associated First Person interview with the first author of the paper.<\/jats:p>","DOI":"10.1242\/jcs.246025","type":"journal-article","created":{"date-parts":[[2020,7,14]],"date-time":"2020-07-14T15:45:14Z","timestamp":1594741514000},"source":"Crossref","is-referenced-by-count":32,"title":["The C-terminal helix of BubR1 is essential for CENP-E-dependent chromosome alignment"],"prefix":"10.1242","volume":"133","author":[{"given":"Thibault","family":"Legal","sequence":"first","affiliation":[{"name":"Wellcome Trust Centre for Cell Biology, School of Biological Sciences, University of Edinburgh 1 , Edinburgh EH9 3BF, Scotland , UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Hayward","sequence":"additional","affiliation":[{"name":"Sir William Dunn School of Pathology, University of Oxford 2 , South Parks Road, Oxford OX1 3RE, \u2008 UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agata","family":"Gluszek-Kustusz","sequence":"additional","affiliation":[{"name":"Wellcome Trust Centre for Cell Biology, School of Biological Sciences, University of Edinburgh 1 , Edinburgh EH9 3BF, Scotland , UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1325-9575","authenticated-orcid":false,"given":"Elizabeth A.","family":"Blackburn","sequence":"additional","affiliation":[{"name":"Wellcome Trust Centre for Cell Biology, School of Biological Sciences, University of Edinburgh 1 , Edinburgh EH9 3BF, Scotland , UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christos","family":"Spanos","sequence":"additional","affiliation":[{"name":"Wellcome Trust Centre for Cell Biology, School of Biological Sciences, University of Edinburgh 1 , Edinburgh EH9 3BF, Scotland , UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5999-1310","authenticated-orcid":false,"given":"Juri","family":"Rappsilber","sequence":"additional","affiliation":[{"name":"Wellcome Trust Centre for Cell Biology, School of Biological Sciences, University of Edinburgh 1 , Edinburgh EH9 3BF, Scotland , UK"},{"name":"Institute of Biotechnology, Technische Universit\u00e4t Berlin 3 Chair of Bioanalytics , , Berlin 10623 , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8344-0500","authenticated-orcid":false,"given":"Ulrike","family":"Gruneberg","sequence":"additional","affiliation":[{"name":"Sir William Dunn School of Pathology, University of Oxford 2 , South Parks Road, Oxford OX1 3RE, \u2008 UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5440-6060","authenticated-orcid":false,"given":"Julie P. 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