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Activation of ERKs is elevated in Ras-driven tumors and RASopathies, and thus is a target for pharmacological inhibition. Regulatory mechanisms of ERK activation have been studied extensively in vitro and in cultured cells, but little in living animals. In this study, we tagged the Caenorhabditis elegans ERK-encoding gene, mpk-1. MPK-1 is ubiquitously expressed with elevated expression in certain contexts. We detected cytosol-to-nuclear translocation of MPK-1 in maturing oocytes and hence validated nuclear translocation as a reporter of some activation events. During patterning of vulval precursor cells (VPCs), MPK-1 is necessary and sufficient for the central cell, P6.p, to assume the primary fate. Yet MPK-1 translocates to the nuclei of all six VPCs in a temporal and concentration gradient centered on P6.p. This observation contrasts with previous results using the ERK nuclear kinase translocation reporter of substrate activation, raising questions about mechanisms and indicators of MPK-1 activation. This system and reagent promise to provide critical insights into the regulation of MPK-1 activation within a complex intercellular signaling network.<\/jats:p>","DOI":"10.1242\/jcs.258456","type":"journal-article","created":{"date-parts":[[2021,8,3]],"date-time":"2021-08-03T03:59:26Z","timestamp":1627963166000},"source":"Crossref","is-referenced-by-count":11,"title":["Nuclear translocation of the tagged endogenous MAPK MPK-1 denotes a subset of activation events in\n                    <i>C<\/i>\n                    .\n                    <i>elegans<\/i>\n                    development"],"prefix":"10.1242","volume":"134","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5343-9119","authenticated-orcid":false,"given":"Neal R.","family":"Rasmussen","sequence":"first","affiliation":[{"name":"Institute of Biosciences and Technology, College of Medicine, Texas A&M Health Science Center, Texas A&M University, Houston, 77030, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0344-7161","authenticated-orcid":false,"given":"David J.","family":"Reiner","sequence":"additional","affiliation":[{"name":"Institute of Biosciences and Technology, College of Medicine, Texas A&M Health Science Center, Texas A&M University, Houston, 77030, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2021,9,3]]},"reference":[{"key":"2021091414572554200_JCS258456C1","doi-asserted-by":"publisher","first-page":"32813","DOI":"10.1074\/jbc.M313656200","article-title":"Subcellular localization determines the protective effects of activated ERK2 against distinct apoptogenic stimuli in myeloid leukemia cells","volume":"279","author":"Ajenjo","year":"2004","journal-title":"J. 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