{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T23:29:53Z","timestamp":1790810993496,"version":"4.1.0"},"reference-count":79,"publisher":"The Company of Biologists","issue":"16","license":[{"start":{"date-parts":[[2021,8,15]],"date-time":"2021-08-15T00:00:00Z","timestamp":1628985600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"funder":[{"DOI":"10.13039\/501100002426","name":"Fondazione Telethon","doi-asserted-by":"crossref","award":["GGP16003"],"award-info":[{"award-number":["GGP16003"]}],"id":[{"id":"10.13039\/501100002426","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100005010","name":"Associazione Italiana per la Ricerca sul Cancro","doi-asserted-by":"crossref","award":["IG-2017-20148"],"award-info":[{"award-number":["IG-2017-20148"]}],"id":[{"id":"10.13039\/501100005010","id-type":"DOI","asserted-by":"crossref"}]},{"name":"European Research Council","award":["951329"],"award-info":[{"award-number":["951329"]}]},{"DOI":"10.13039\/501100001824","name":"Grantov\u00e1 Agentura \u010cesk\u00e9 Republiky","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100001824","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,8,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>Components of the intraflagellar transport (IFT) system that regulates the assembly of the primary cilium are co-opted by the non-ciliated T cell to orchestrate polarized endosome recycling and to sustain signaling during immune synapse formation. Here, we investigated the potential role of Bardet\u2013Biedl syndrome 1 protein (BBS1), an essential core component of the BBS complex that cooperates with the IFT system in ciliary protein trafficking, in the assembly of the T cell synapse. We demonstrated that BBS1 allows for centrosome polarization towards the immune synapse. This function is achieved through the clearance of centrosomal F-actin and its positive regulator WASH1 (also known as WASHC1), a process that we demonstrated to be dependent on the proteasome. We show that BBS1 regulates this process by coupling the 19S proteasome regulatory subunit to the microtubule motor dynein for its transport to the centrosome. Our data identify the ciliopathy-related protein BBS1 as a new player in T cell synapse assembly that functions upstream of the IFT system to set the stage for polarized vesicular trafficking and sustained signaling.<\/jats:p>\n               <jats:p>This article has an associated First Person interview with the first author of the paper.<\/jats:p>","DOI":"10.1242\/jcs.258462","type":"journal-article","created":{"date-parts":[[2021,7,12]],"date-time":"2021-07-12T13:15:51Z","timestamp":1626095751000},"source":"Crossref","is-referenced-by-count":27,"title":["The Bardet\u2013Biedl syndrome complex component BBS1 controls T cell polarity during immune synapse assembly"],"prefix":"10.1242","volume":"134","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4853-2298","authenticated-orcid":false,"given":"Chiara","family":"Cassioli","sequence":"first","affiliation":[{"name":"Department of Life Sciences, University of Siena, 53100, Siena, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3199-9868","authenticated-orcid":false,"given":"Anna","family":"Onnis","sequence":"additional","affiliation":[{"name":"Department of Life Sciences, University of Siena, 53100, Siena, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesca","family":"Finetti","sequence":"additional","affiliation":[{"name":"Department of Life Sciences, University of Siena, 53100, Siena, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3653-8972","authenticated-orcid":false,"given":"Nagaja","family":"Capitani","sequence":"additional","affiliation":[{"name":"Department of Life Sciences, University of Siena, 53100, Siena, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8144-7186","authenticated-orcid":false,"given":"Jlenia","family":"Brunetti","sequence":"additional","affiliation":[{"name":"Department of Medical Biotechnologies, University of Siena, 53100, Siena, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3050-7633","authenticated-orcid":false,"given":"Ewoud B.","family":"Compeer","sequence":"additional","affiliation":[{"name":"Kennedy Institute of Rheumatology, University of Oxford, OX3 7FY, Oxford, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Veronika","family":"Niederlova","sequence":"additional","affiliation":[{"name":"Institute of Molecular Genetics of the Czech Academy of Sciences, 142 20, Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2735-3311","authenticated-orcid":false,"given":"Ondrej","family":"Stepanek","sequence":"additional","affiliation":[{"name":"Institute of Molecular Genetics of the Czech Academy of Sciences, 142 20, Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4983-6389","authenticated-orcid":false,"given":"Michael L.","family":"Dustin","sequence":"additional","affiliation":[{"name":"Kennedy Institute of Rheumatology, University of Oxford, OX3 7FY, Oxford, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4414-6744","authenticated-orcid":false,"given":"Cosima T.","family":"Baldari","sequence":"additional","affiliation":[{"name":"Department of Life Sciences, University of Siena, 53100, Siena, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2021,8,23]]},"reference":[{"key":"2021090108245530100_JCS258462C1","doi-asserted-by":"publisher","first-page":"103","DOI":"10.1146\/annurev-immunol-042617-053429","article-title":"Cell biology of T cell receptor expression and regulation","volume":"36","author":"Alcover","year":"2018","journal-title":"Annu. 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