{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T03:45:24Z","timestamp":1791949524296,"version":"4.3.4"},"reference-count":70,"publisher":"The Company of Biologists","issue":"6","license":[{"start":{"date-parts":[[2022,3,15]],"date-time":"2022-03-15T00:00:00Z","timestamp":1647302400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"funder":[{"DOI":"10.13039\/100000069","name":"National Institute of Arthritis and Musculoskeletal and Skin Diseases","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100000069","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["R21AR076514"],"award-info":[{"award-number":["R21AR076514"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,3,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Emerin is an integral nuclear envelope protein that participates in the maintenance of nuclear shape. When mutated or absent, emerin causes X-linked Emery-Dreifuss muscular dystrophy (EDMD). To understand how emerin takes part in molecular \u00ad\u00adscaffolding at the nuclear envelope and helps protect the nucleus against mechanical stress, we established its nanoscale organization using single-molecule tracking and super-resolution microscopy. We show that emerin monomers form localized oligomeric nanoclusters stabilized by both lamin A\/C and the SUN1-containing linker of nucleoskeleton and cytoskeleton (LINC) complex. Interactions of emerin with nuclear actin and BAF (also known as BANF1) additionally modulate its membrane mobility and its ability to oligomerize. In nuclei subjected to mechanical challenges, the mechanotransduction functions of emerin are coupled to changes in its oligomeric state, and the incremental self-assembly of emerin determines nuclear shape adaptation against mechanical forces. We also show that the abnormal nuclear envelope deformations induced by EDMD emerin mutants stem from improper formation of lamin A\/C and LINC complex-stabilized emerin oligomers. These findings place emerin at the center of the molecular processes that regulate nuclear shape remodeling in response to mechanical challenges.<\/jats:p>","DOI":"10.1242\/jcs.258969","type":"journal-article","created":{"date-parts":[[2022,2,18]],"date-time":"2022-02-18T04:26:09Z","timestamp":1645158369000},"source":"Crossref","is-referenced-by-count":39,"title":["Emerin self-assembly and nucleoskeletal coupling regulate nuclear envelope mechanics against stress"],"prefix":"10.1242","volume":"135","author":[{"given":"Anthony","family":"Fernandez","sequence":"first","affiliation":[{"name":"University of Southern California 1 Department of Biological Sciences , , 1050 Childs Way, Los Angeles, CA 90089 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Markville","family":"Bautista","sequence":"additional","affiliation":[{"name":"University of Southern California 2 Department of Chemistry , , 1050 Childs Way, Los Angeles, CA 90089 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liying","family":"Wu","sequence":"additional","affiliation":[{"name":"University of Southern California 1 Department of Biological Sciences , , 1050 Childs Way, Los Angeles, CA 90089 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4272-3616","authenticated-orcid":false,"given":"Fabien","family":"Pinaud","sequence":"additional","affiliation":[{"name":"University of Southern California 1 Department of Biological Sciences , , 1050 Childs Way, Los Angeles, CA 90089 , USA"},{"name":"University of Southern California 2 Department of Chemistry , , 1050 Childs Way, Los Angeles, CA 90089 , USA"},{"name":"University of Southern California 3 Department of Physics and Astronomy , , 1050 Childs Way, Los Angeles, CA 90089 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2022,3,30]]},"reference":[{"key":"2024061307342179700_JCS258969C1","doi-asserted-by":"publisher","first-page":"34","DOI":"10.1016\/j.bpj.2015.11.014","article-title":"Nesprin-2G, a component of the nuclear LINC complex, is subject to myosin-dependent tension","volume":"110","author":"Arsenovic","year":"2016","journal-title":"Biophys. 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