{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T14:34:59Z","timestamp":1785854099613,"version":"3.56.0"},"reference-count":36,"publisher":"The Company of Biologists","issue":"6","license":[{"start":{"date-parts":[[2022,3,15]],"date-time":"2022-03-15T00:00:00Z","timestamp":1647302400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"crossref","award":["JP21K16125"],"award-info":[{"award-number":["JP21K16125"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100007449","name":"Takeda Science Foundation","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100007449","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,3,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>Mucociliary clearance, which is conducted by beating cilia cooperating with the surface mucous layer, is a major host defense mechanism of the airway epithelium. Ezrin, a crosslinker between membrane proteins and the actin cytoskeleton, is located in microvilli and around the basal bodies in airway ciliary cells. It is also likely that ezrin plays an important role in apical localization of \u03b22 adrenergic receptor (\u03b22AR) in airway ciliary cells. Here, we studied the physiological roles of ezrin by using trachea and airway epithelial cells prepared from ezrin-knockdown (Vil2kd\/kd) mice. The trachea and airway ciliary cells of Vil2kd\/kd mice presented a normal morphology and basal body orientation, suggesting that ezrin is not directly involved in development and planar cell polarity of cilia. Procaterol stimulates ciliary beating (frequency and amplitude) via \u03b22AR in the airway ciliary cells. In the Vil2kd\/kd mice, airway ciliary beating stimulated with procaterol was partly inhibited due to the impairment of cell surface expression of \u03b22AR. These results suggest that ezrin regulates the beating of airway ciliary cells by promoting the apical surface localization of \u03b22AR.<\/jats:p>\n               <jats:p>This article has an associated First Person interview with the first author of the paper.<\/jats:p>","DOI":"10.1242\/jcs.259201","type":"journal-article","created":{"date-parts":[[2022,2,8]],"date-time":"2022-02-08T12:16:01Z","timestamp":1644322561000},"source":"Crossref","is-referenced-by-count":13,"title":["Ezrin knockdown reduces procaterol-stimulated ciliary beating without morphological changes in mouse airway cilia"],"prefix":"10.1242","volume":"135","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9552-7494","authenticated-orcid":false,"given":"Kotoku","family":"Kawaguchi","sequence":"first","affiliation":[{"name":"Department of Molecular Physiology, College of Pharmaceutical Sciences, Ritsumeikan University, Shiga 525-8577, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shogo","family":"Nakayama","sequence":"additional","affiliation":[{"name":"Laboratory for Lung Development and Regeneration, Riken Center for Biosystems Dynamics Research (BDR), Kobe 650-0047, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daichi","family":"Saito","sequence":"additional","affiliation":[{"name":"Department of Molecular Physiology, College of Pharmaceutical Sciences, Ritsumeikan University, Shiga 525-8577, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haruka","family":"Kogiso","sequence":"additional","affiliation":[{"name":"Research Unit for Epithelial Physiology, Research Organization of Science and Technology, Ritsumeikan University, Shiga 525-8577, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kasane","family":"Yasuoka","sequence":"additional","affiliation":[{"name":"Department of Molecular Physiology, College of Pharmaceutical Sciences, Ritsumeikan University, Shiga 525-8577, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshinori","family":"Marunaka","sequence":"additional","affiliation":[{"name":"Research Unit for Epithelial Physiology, Research Organization of Science and Technology, Ritsumeikan University, Shiga 525-8577, Japan"},{"name":"Department of Molecular Cell Physiology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan"},{"name":"Medical Research Institute, Kyoto Industrial Health Association, Kyoto 604-8472, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7093-5520","authenticated-orcid":false,"given":"Takashi","family":"Nakahari","sequence":"additional","affiliation":[{"name":"Research Unit for Epithelial Physiology, Research Organization of Science and Technology, Ritsumeikan University, Shiga 525-8577, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1443-8970","authenticated-orcid":false,"given":"Shinji","family":"Asano","sequence":"additional","affiliation":[{"name":"Department of Molecular Physiology, College of Pharmaceutical Sciences, Ritsumeikan University, Shiga 525-8577, Japan"},{"name":"Research Unit for Epithelial Physiology, Research Organization of Science and Technology, Ritsumeikan University, Shiga 525-8577, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2022,3,16]]},"reference":[{"key":"2022031611424260800_JCS259201C1","doi-asserted-by":"publisher","first-page":"470","DOI":"10.1002\/path.1652","article-title":"Cilia-related diseases","volume":"204","author":"Afzelius","year":"2004","journal-title":"J. Pathol."},{"key":"2022031611424260800_JCS259201C2","doi-asserted-by":"publisher","first-page":"21480","DOI":"10.1074\/jbc.M112245200","article-title":"Reciprocal protein kinase A regulatory interactions between cystic fibrosis transmembrane conductance regulator and Na+\/H+ exchanger isoform 3 in a renal polarized epithelial cell model","volume":"277","author":"Bagorda","year":"2002","journal-title":"J. Biol. Chem."},{"key":"2022031611424260800_JCS259201C3","doi-asserted-by":"publisher","first-page":"720","DOI":"10.1016\/j.exer.2005.09.013","article-title":"Microvilli defects in retinas of ezrin knockout mice","volume":"82","author":"Bonilha","year":"2006","journal-title":"Exp. Eye Res."},{"key":"2022031611424260800_JCS259201C4","doi-asserted-by":"publisher","first-page":"11924","DOI":"10.1073\/pnas.1103418108","article-title":"Ezrin-mediated apical integrity is required for intestinal homeostasis","volume":"108","author":"Casaletto","year":"2011","journal-title":"Proc. Natl. Sci. USA"},{"key":"2022031611424260800_JCS259201C5","doi-asserted-by":"publisher","first-page":"110","DOI":"10.1165\/rcmb.2005-0417OC","article-title":"Ciliary beat frequency is maintained at a maximal rate in the small airways of mouse lung slices","volume":"35","author":"Delmotte","year":"2006","journal-title":"Am. J. Respir. Cell Mol. Biol."},{"key":"2022031611424260800_JCS259201C36","doi-asserted-by":"publisher","first-page":"2315","DOI":"10.1074\/jbc.274.4.2315","article-title":"Normal development of mice and unimpaired cell adhesion\/cell motility\/actin-based cytoskeleton without compensatory up-regulation of ezrin or radixin in moesin gene knockout","volume":"274","author":"Doi","year":"1999","journal-title":"J. Biol. 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Cell Sci."},{"key":"2022031611424260800_JCS259201C9","doi-asserted-by":"publisher","first-page":"535","DOI":"10.1083\/jcb.200301105","article-title":"A novel human protein of the maternal centriole is required for the final stages of cytokinesis and entry into S phase","volume":"161","author":"Gromley","year":"2003","journal-title":"J. Cell Biol."},{"key":"2022031611424260800_JCS259201C10","doi-asserted-by":"publisher","first-page":"41","DOI":"10.1038\/ki.2012.308","article-title":"Ezrin, a membrane cytoskeletal cross-linker, is essential for the regulation of phosphate and calcium homeostasis","volume":"83","author":"Hatano","year":"2013","journal-title":"Kidney Int."},{"key":"2022031611424260800_JCS259201C11","doi-asserted-by":"publisher","first-page":"1660","DOI":"10.1002\/hep.27565","article-title":"Knockdown of ezrin causes intrahepatic cholestasis by the dysregulation of bile fluidity in the bile duct epithelium in mice","volume":"61","author":"Hatano","year":"2015","journal-title":"Hepatology"},{"key":"2022031611424260800_JCS259201C12","doi-asserted-by":"publisher","first-page":"571","DOI":"10.1083\/jcb.201601023","article-title":"Multiciliated cell basal bodies align in stereotypical patterns coordinated by the apical cytoskeleton","volume":"214","author":"Herawati","year":"2016","journal-title":"J. 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Care Med."}],"container-title":["Journal of Cell Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.biologists.com\/jcs\/article-pdf\/doi\/10.1242\/jcs.259201\/2129839\/jcs259201.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/journals.biologists.com\/jcs\/article-pdf\/doi\/10.1242\/jcs.259201\/2136477\/jcs259201.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/journals.biologists.com\/jcs\/article-pdf\/doi\/10.1242\/jcs.259201\/2136477\/jcs259201.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,16]],"date-time":"2022-03-16T11:43:04Z","timestamp":1647430984000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.biologists.com\/jcs\/article\/135\/6\/jcs259201\/274729\/Ezrin-knockdown-reduces-procaterol-stimulated"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,3,15]]},"references-count":36,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2022,3,15]]}},"URL":"https:\/\/doi.org\/10.1242\/jcs.259201","relation":{},"ISSN":["0021-9533","1477-9137"],"issn-type":[{"value":"0021-9533","type":"print"},{"value":"1477-9137","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,3,15]]},"article-number":"jcs259201"}}