{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T16:41:52Z","timestamp":1789058512910,"version":"build-2803163510"},"reference-count":48,"publisher":"The Company of Biologists","issue":"6","license":[{"start":{"date-parts":[[2022,3,23]],"date-time":"2022-03-23T00:00:00Z","timestamp":1647993600000},"content-version":"vor","delay-in-days":8,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"crossref","award":["213470\/Z\/18\/Z"],"award-info":[{"award-number":["213470\/Z\/18\/Z"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100000288","name":"Royal Society","doi-asserted-by":"crossref","award":["213470\/Z\/18\/Z"],"award-info":[{"award-number":["213470\/Z\/18\/Z"]}],"id":[{"id":"10.13039\/501100000288","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Human Frontier Science Program","award":["RGY0070\/2019"],"award-info":[{"award-number":["RGY0070\/2019"]}]},{"DOI":"10.13039\/501100000883","name":"University of Bristol","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100000883","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,3,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Formation of healthy mammalian eggs from oocytes requires specialised F-actin structures. F-actin disruption produces aneuploid eggs, which are a leading cause of human embryo deaths, genetic disorders and infertility. We found that oocytes contain prominent nuclear F-actin structures that are correlated with meiotic developmental capacity. We demonstrate that nuclear F-actin is a conserved feature of healthy mammalian oocytes and declines significantly with female reproductive ageing. Actin monomers used for nuclear F-actin assembly are sourced from an excess pool in the oocyte cytoplasm. Increasing monomeric G-actin transfer from the cytoplasm to the nucleus or directly enriching the nucleus with monomers led to assembly of stable nuclear F-actin bundles that significantly restrict chromatin mobility. By contrast, reducing G-actin monomer transfer by blocking nuclear import triggered assembly of a dense cytoplasmic F-actin network that is incompatible with healthy oocyte development. Overall, our data suggest that the large oocyte nucleus helps to maintain cytoplasmic F-actin organisation and that defects in this function are linked with reproductive age-related female infertility.<\/jats:p>\n                  <jats:p>This article has an associated First Person interview with Federica Giannini, joint first author of the paper.<\/jats:p>","DOI":"10.1242\/jcs.259807","type":"journal-article","created":{"date-parts":[[2022,2,3]],"date-time":"2022-02-03T05:31:06Z","timestamp":1643866266000},"source":"Crossref","is-referenced-by-count":29,"title":["The prophase oocyte nucleus is a homeostatic G-actin buffer"],"prefix":"10.1242","volume":"135","author":[{"given":"Kathleen","family":"Scheffler","sequence":"first","affiliation":[{"name":"University of Bristol 1 School of Biochemistry , , Bristol BS8 1TD , UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Federica","family":"Giannini","sequence":"additional","affiliation":[{"name":"University of Bristol 1 School of Biochemistry , , Bristol BS8 1TD , UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tom","family":"Lemonnier","sequence":"additional","affiliation":[{"name":"University of Bristol 1 School of Biochemistry , , Bristol BS8 1TD , UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0702-6356","authenticated-orcid":false,"given":"Binyam","family":"Mogessie","sequence":"additional","affiliation":[{"name":"University of Bristol 1 School of Biochemistry , , Bristol BS8 1TD , UK"},{"name":"Yale University 2 Department of Molecular, Cellular and Developmental Biology , , New Haven, CT 06511 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2022,3,23]]},"reference":[{"key":"2024080908260917600_JCS259807C1","doi-asserted-by":"publisher","first-page":"13200","DOI":"10.1073\/pnas.1902035116","article-title":"Regulation of nuclear architecture, mechanics, and nucleocytoplasmic shuttling of epigenetic factors by cell geometric constraints","volume":"116","author":"Alisafaei","year":"2019","journal-title":"Proc. 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