{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T22:03:48Z","timestamp":1790287428068,"version":"4.1.0"},"reference-count":43,"publisher":"The Company of Biologists","issue":"14","license":[{"start":{"date-parts":[[2022,7,20]],"date-time":"2022-07-20T00:00:00Z","timestamp":1658275200000},"content-version":"vor","delay-in-days":5,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"crossref","award":["104575"],"award-info":[{"award-number":["104575"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"crossref","award":["215523"],"award-info":[{"award-number":["215523"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Edward Penley Abraham Scholarship"},{"DOI":"10.13039\/100007100","name":"Sandler Foundation","doi-asserted-by":"crossref","award":["7029760"],"award-info":[{"award-number":["7029760"]}],"id":[{"id":"10.13039\/100007100","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100008069","name":"University of California, San Francisco","doi-asserted-by":"crossref","award":["2017078"],"award-info":[{"award-number":["2017078"]}],"id":[{"id":"10.13039\/100008069","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100008069","name":"University of California, San Francisco","doi-asserted-by":"crossref","award":["203855"],"award-info":[{"award-number":["203855"]}],"id":[{"id":"10.13039\/100008069","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100009729","name":"Ludwig Institute for Cancer Research","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100009729","id-type":"DOI","asserted-by":"crossref"}]},{"name":"University of Oxford"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,7,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Centrioles are composed of a central cartwheel tethered to nine-fold symmetric microtubule (MT) blades. The centriole cartwheel and MTs are thought to grow from opposite ends of these organelles, so it is unclear how they coordinate their assembly. We previously showed that in Drosophila embryos an oscillation of Polo-like kinase 4 (Plk4) helps to initiate and time the growth of the cartwheel at the proximal end. Here, in the same model, we show that CP110 and Cep97 form a complex close to the distal-end of the centriole MTs whose levels rise and fall as the new centriole MTs grow, in a manner that appears to be entrained by the core cyclin-dependent kinase (Cdk)\u2013Cyclin oscillator that drives the nuclear divisions in these embryos. These CP110 and Cep97 dynamics, however, do not appear to time the period of centriole MT growth directly. Instead, we find that changing the levels of CP110 and Cep97 appears to alter the Plk4 oscillation and the growth of the cartwheel at the proximal end. These findings reveal an unexpected potential crosstalk between factors normally concentrated at opposite ends of the growing centrioles, which might help to coordinate centriole growth.<\/jats:p>\n                  <jats:p>This article has an associated First Person interview with the first authors of the paper.<\/jats:p>","DOI":"10.1242\/jcs.260015","type":"journal-article","created":{"date-parts":[[2022,6,16]],"date-time":"2022-06-16T04:38:00Z","timestamp":1655354280000},"source":"Crossref","is-referenced-by-count":12,"title":["Centriole distal-end proteins CP110 and Cep97 influence centriole cartwheel growth at the proximal end"],"prefix":"10.1242","volume":"135","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1673-0596","authenticated-orcid":false,"given":"Mustafa G.","family":"Aydogan","sequence":"first","affiliation":[{"name":"Sir William Dunn School of Pathology, University of Oxford 1 , Oxford OX1 3RE , UK"},{"name":"University of California, San Francisco 2 Department of Biochemistry and Biophysics , , San Francisco, CA 94158 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1622-8678","authenticated-orcid":false,"given":"Laura E.","family":"Hankins","sequence":"additional","affiliation":[{"name":"Sir William Dunn School of Pathology, University of Oxford 1 , Oxford OX1 3RE , UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7244-5610","authenticated-orcid":false,"given":"Thomas L.","family":"Steinacker","sequence":"additional","affiliation":[{"name":"Sir William Dunn School of Pathology, University of Oxford 1 , Oxford OX1 3RE , UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2458-0447","authenticated-orcid":false,"given":"Mohammad","family":"Mofatteh","sequence":"additional","affiliation":[{"name":"Sir William Dunn School of Pathology, University of Oxford 1 , Oxford OX1 3RE , UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4057-0123","authenticated-orcid":false,"given":"Saroj","family":"Saurya","sequence":"additional","affiliation":[{"name":"Sir William Dunn School of Pathology, University of Oxford 1 , Oxford OX1 3RE , UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6292-4183","authenticated-orcid":false,"given":"Alan","family":"Wainman","sequence":"additional","affiliation":[{"name":"Sir William Dunn School of Pathology, University of Oxford 1 , Oxford OX1 3RE , UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5327-8466","authenticated-orcid":false,"given":"Siu-Shing","family":"Wong","sequence":"additional","affiliation":[{"name":"Sir William Dunn School of Pathology, University of Oxford 1 , Oxford OX1 3RE , UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Lu","sequence":"additional","affiliation":[{"name":"Ludwig Institute for Cancer Research 3 , Nuffield Department of Clinical Medicine , , Oxford OX3 7DQ , UK"},{"name":"University of Oxford 3 , Nuffield Department of Clinical Medicine , , Oxford OX3 7DQ , UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4463-1165","authenticated-orcid":false,"given":"Felix Y.","family":"Zhou","sequence":"additional","affiliation":[{"name":"Ludwig Institute for Cancer Research 3 , Nuffield Department of Clinical Medicine , , Oxford OX3 7DQ , UK"},{"name":"University of Oxford 3 , Nuffield Department of Clinical Medicine , , Oxford OX3 7DQ , UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4689-1297","authenticated-orcid":false,"given":"Jordan W.","family":"Raff","sequence":"additional","affiliation":[{"name":"Sir William Dunn School of Pathology, University of Oxford 1 , Oxford OX1 3RE , UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2022,7,20]]},"reference":[{"key":"2022072708533167300_JCS260015C1","doi-asserted-by":"publisher","first-page":"e50130","DOI":"10.7554\/eLife.50130","article-title":"Evidence that a positive feedback loop drives centrosome maturation in fly embryos","volume":"8","author":"Alvarez-Rodrigo","year":"2019","journal-title":"ELife"},{"key":"2022072708533167300_JCS260015C2","doi-asserted-by":"publisher","first-page":"jcs258987.","DOI":"10.1242\/jcs.258987","article-title":"Ana1 helps recruit Polo to centrioles to promote mitotic PCM assembly and centriole elongation","volume":"134","author":"Alvarez-Rodrigo","year":"2021","journal-title":"J. Cell Sci."},{"key":"2022072708533167300_JCS260015C3","doi-asserted-by":"publisher","first-page":"1253","DOI":"10.1042\/BST20160116","article-title":"The PLK4\u2013STIL\u2013SAS-6 module at the core of centriole duplication","volume":"44","author":"Arquint","year":"2016","journal-title":"Biochem. Soc. Trans."},{"key":"2022072708533167300_JCS260015C4","doi-asserted-by":"publisher","first-page":"1233","DOI":"10.1083\/jcb.201801014","article-title":"A homeostatic clock sets daughter centriole size in flies","volume":"217","author":"Aydogan","year":"2018","journal-title":"J. Cell Biol."},{"key":"2022072708533167300_JCS260015C5","doi-asserted-by":"publisher","first-page":"1566","DOI":"10.1016\/j.cell.2020.05.018","article-title":"An autonomous oscillation times and executes centriole biogenesis","volume":"181","author":"Aydogan","year":"2020","journal-title":"Cell"},{"key":"2022072708533167300_JCS260015C6","doi-asserted-by":"publisher","first-page":"23","DOI":"10.1146\/annurev-cellbio-100616-060454","article-title":"Centriole biogenesis: from identifying the characters to understanding the plot","volume":"33","author":"Banterle","year":"2017","journal-title":"Annu. Rev. Cell Dev. Biol."},{"key":"2022072708533167300_JCS260015C7","doi-asserted-by":"publisher","first-page":"1032","DOI":"10.1016\/j.cell.2008.05.039","article-title":"Centrosome amplification can initiate tumorigenesis in flies","volume":"133","author":"Basto","year":"2008","journal-title":"Cell"},{"key":"2022072708533167300_JCS260015C9","doi-asserted-by":"publisher","first-page":"691","DOI":"10.1146\/annurev-biochem-013118-111153","article-title":"Mechanism and regulation of centriole and cilium biogenesis","volume":"88","author":"Breslow","year":"2019","journal-title":"Annu. Rev. Biochem."},{"key":"2022072708533167300_JCS260015C10","doi-asserted-by":"publisher","first-page":"2501","DOI":"10.1242\/jcs.186338","article-title":"CEP295 interacts with microtubules and is required for centriole elongation","volume":"129","author":"Chang","year":"2016","journal-title":"J. Cell Sci."},{"key":"2022072708533167300_JCS260015C11","doi-asserted-by":"publisher","first-page":"339","DOI":"10.1016\/S1534-5807(02)00258-7","article-title":"CP110, a cell cycle-dependent CDK substrate, regulates centrosome duplication in human cells","volume":"3","author":"Chen","year":"2002","journal-title":"Dev. Cell"},{"key":"2022072708533167300_JCS260015C13","doi-asserted-by":"publisher","first-page":"2591","DOI":"10.1016\/j.cub.2015.08.039","article-title":"A short CEP135 splice isoform controls centriole duplication","volume":"25","author":"Dahl","year":"2015","journal-title":"Curr. Biol."},{"key":"2022072708533167300_JCS260015C14","doi-asserted-by":"publisher","first-page":"502","DOI":"10.1016\/j.cub.2012.01.046","article-title":"Klp10A, a microtubule-depolymerizing kinesin-13, cooperates with CP110 to control Drosophila centriole length","volume":"22","author":"Delgehyr","year":"2012","journal-title":"Curr. Biol."},{"key":"2022072708533167300_JCS260015C15","doi-asserted-by":"publisher","first-page":"399","DOI":"10.1016\/j.devcel.2016.07.023","article-title":"Waves of Cdk1 activity in S phase synchronize the cell cycle in drosophila embryos","volume":"38","author":"Deneke","year":"2016","journal-title":"Dev. Cell"},{"key":"2022072708533167300_JCS260015C16","doi-asserted-by":"publisher","first-page":"e224","DOI":"10.1371\/journal.pbio.0060224","article-title":"A genome-wide RNAi screen to dissect centriole duplication and centrosome maturation in Drosophila","volume":"6","author":"Dobbelaere","year":"2008","journal-title":"PLoS Biol."},{"key":"2022072708533167300_JCS260015C17","doi-asserted-by":"publisher","first-page":"3045","DOI":"10.1016\/j.cub.2020.05.078","article-title":"Cep97 is required for centriole structural integrity and cilia formation in Drosophila","volume":"30","author":"Dobbelaere","year":"2020","journal-title":"Curr. Biol."},{"key":"2022072708533167300_JCS260015C18","doi-asserted-by":"publisher","first-page":"269","DOI":"10.1146\/annurev-genet-111212-133531","article-title":"From egg to gastrula: how the cell cycle is remodeled during the Drosophila mid-blastula transition","volume":"48","author":"Farrell","year":"2014","journal-title":"Annu. Rev. Genet."},{"key":"2022072708533167300_JCS260015C19","doi-asserted-by":"publisher","first-page":"20130460","DOI":"10.1098\/rstb.2013.0460","article-title":"The centriole duplication cycle","volume":"369","author":"F\u0131rat-Karalar","year":"2014","journal-title":"Philos. Trans. R. Soc. B Biol. Sci."},{"key":"2022072708533167300_JCS260015C20","doi-asserted-by":"publisher","first-page":"785","DOI":"10.1083\/jcb.201305109","article-title":"CP110 exhibits novel regulatory activities during centriole assembly in Drosophila","volume":"203","author":"Franz","year":"2013","journal-title":"J. Cell Biol."},{"key":"2022072708533167300_JCS260015C21","doi-asserted-by":"publisher","first-page":"R1054","DOI":"10.1016\/j.cub.2017.08.009","article-title":"A combined 3D-SIM\/SMLM approach allows centriole proteins to be localized with a precision of \u223c4\u20135 nm","volume":"27","author":"Gartenmann","year":"2017","journal-title":"Curr. Biol."},{"key":"2022072708533167300_JCS260015C22","doi-asserted-by":"publisher","first-page":"1169","DOI":"10.1083\/jcb.201802084","article-title":"Fast and furious...or not, Plk4 dictates the pace","volume":"217","author":"Gemble","year":"2018","journal-title":"J. Cell Biol."},{"key":"2022072708533167300_JCS260015C45","doi-asserted-by":"publisher","first-page":"1620","DOI":"10.1016\/j.cub.2013.06.061","article-title":"Native architecture of the centriole proximal region reveals features underlying its 9-fold radial symmetry","volume":"23","author":"Guichard","year":"2013","journal-title":"Curr. Biol."},{"key":"2022072708533167300_JCS260015C24","doi-asserted-by":"publisher","first-page":"190","DOI":"10.1016\/j.devcel.2007.07.002","article-title":"Plk4-induced centriole biogenesis in human cells","volume":"13","author":"Kleylein-Sohn","year":"2007","journal-title":"Dev. Cell"},{"key":"2022072708533167300_JCS260015C25","doi-asserted-by":"publisher","first-page":"1012","DOI":"10.1016\/j.cub.2009.05.018","article-title":"Overly long centrioles and defective cell division upon excess of the SAS-4-related protein CPAP","volume":"19","author":"Kohlmaier","year":"2009","journal-title":"Curr. Biol."},{"key":"2022072708533167300_JCS260015C26","doi-asserted-by":"publisher","first-page":"e201910019","DOI":"10.1083\/jcb.201910019","article-title":"Prolonged mitosis results in structurally aberrant and over-elongated centrioles","volume":"219","author":"Kong","year":"2020","journal-title":"J. Cell Biol."},{"key":"2022072708533167300_JCS260015C27","doi-asserted-by":"publisher","first-page":"eaaz4137","DOI":"10.1126\/sciadv.aaz4137","article-title":"A helical inner scaffold provides a structural basis for centriole cohesion","volume":"6","author":"Le Guennec","year":"2020","journal-title":"Sci. Adv."},{"key":"2022072708533167300_JCS260015C28","doi-asserted-by":"publisher","first-page":"1225","DOI":"10.1080\/15384101.2017.1325555","article-title":"PLK4 phosphorylation of CP110 is required for efficient centriole assembly","volume":"16","author":"Lee","year":"2017","journal-title":"Cell Cycle"},{"key":"2022072708533167300_JCS260015C29","doi-asserted-by":"publisher","first-page":"1141","DOI":"10.1038\/emboj.2013.56","article-title":"Human microcephaly protein CEP135 binds to hSAS\u20136 and CPAP, and is required for centriole assembly","volume":"32","author":"Lin","year":"2013","journal-title":"EMBO J."},{"key":"2022072708533167300_JCS260015C30","doi-asserted-by":"publisher","first-page":"eaaq1392","DOI":"10.1126\/science.aaq1392","article-title":"Observing the cell in its native state: imaging subcellular dynamics in multicellular organisms","volume":"360","author":"Liu","year":"2018","journal-title":"Science"},{"key":"2022072708533167300_JCS260015C31","doi-asserted-by":"publisher","first-page":"513","DOI":"10.3390\/biology10060513","article-title":"Temporal gradients controlling embryonic cell cycle","volume":"10","author":"Liu","year":"2021","journal-title":"Biology"},{"key":"2022072708533167300_JCS260015C32","doi-asserted-by":"publisher","first-page":"e02678","DOI":"10.7554\/eLife.02678","article-title":"Mechanisms of organelle biogenesis govern stochastic fluctuations in organelle abundance","volume":"3","author":"Mukherji","year":"2014","journal-title":"ELife"},{"key":"2022072708533167300_JCS260015C33","doi-asserted-by":"publisher","first-page":"1276","DOI":"10.1016\/j.cub.2014.04.023","article-title":"Asterless licenses daughter centrioles to duplicate for the first time in Drosophila embryos","volume":"24","author":"Novak","year":"2014","journal-title":"Curr. Biol."},{"key":"2022072708533167300_JCS260015C34","doi-asserted-by":"publisher","first-page":"2514","DOI":"10.1242\/jcs.186460","article-title":"Drosophila Ana1 is required for centrosome assembly and centriole elongation","volume":"129","author":"Saurya","year":"2016","journal-title":"J. Cell Sci."},{"key":"2022072708533167300_JCS260015C35","doi-asserted-by":"publisher","first-page":"1005","DOI":"10.1016\/j.cub.2009.05.016","article-title":"Control of centriole length by CPAP and CP110","volume":"19","author":"Schmidt","year":"2009","journal-title":"Curr. Biol."},{"key":"2022072708533167300_JCS260015C36","doi-asserted-by":"publisher","first-page":"362","DOI":"10.1016\/j.devcel.2016.04.024","article-title":"Centriolar CPAP\/SAS-4 imparts slow processive microtubule growth","volume":"37","author":"Sharma","year":"2016","journal-title":"Dev. Cell"},{"key":"2022072708533167300_JCS260015C37","doi-asserted-by":"publisher","first-page":"89","DOI":"10.1016\/j.sbi.2020.10.011","article-title":"Centriole length control","volume":"66","author":"Sharma","year":"2021","journal-title":"Curr. Opin. Struct. Biol."},{"key":"2022072708533167300_JCS260015C38","doi-asserted-by":"publisher","first-page":"678","DOI":"10.1016\/j.cell.2007.06.027","article-title":"Cep97 and CP110 suppress a cilia assembly program","volume":"130","author":"Spektor","year":"2007","journal-title":"Cell"},{"key":"2022072708533167300_JCS260015C39","doi-asserted-by":"publisher","first-page":"80","DOI":"10.1016\/j.ymeth.2016.09.016","article-title":"TrackMate: an open and extensible platform for single-particle tracking","volume":"115","author":"Tinevez","year":"2017","journal-title":"Methods"},{"key":"2022072708533167300_JCS260015C40","doi-asserted-by":"publisher","first-page":"958","DOI":"10.1093\/bioinformatics\/btv687","article-title":"FoCuS-point: software for STED fluorescence correlation and time-gated single photon counting","volume":"32","author":"Waithe","year":"2016","journal-title":"Bioinformatics"},{"key":"2022072708533167300_JCS260015C41","doi-asserted-by":"publisher","first-page":"20130457","DOI":"10.1098\/rstb.2013.0457","article-title":"Centriole structure","volume":"369","author":"Winey","year":"2014","journal-title":"Philos. Trans. R. Soc. B Biol. Sci."},{"key":"2022072708533167300_JCS260015C46","doi-asserted-by":"publisher","first-page":"e110891","DOI":"10.15252\/embj.2022110891","article-title":"Centrioles generate a local pulse of Polo\/PLK1 activity to initiate mitotic centrosome assembly","volume":"41","author":"Wong","year":"2022","journal-title":"EMBO J"},{"key":"2022072708533167300_JCS260015C42","doi-asserted-by":"publisher","first-page":"1491","DOI":"10.1242\/dev.130120","article-title":"Centrosomal protein CP110 controls maturation of the mother centriole during cilia biogenesis","volume":"143","author":"Yadav","year":"2016","journal-title":"Development"},{"key":"2022072708533167300_JCS260015C43","doi-asserted-by":"publisher","first-page":"2023","DOI":"10.1038\/s41467-018-04469-1","article-title":"Super-resolution architecture of mammalian centriole distal appendages reveals distinct blade and matrix functional components","volume":"9","author":"Yang","year":"2018","journal-title":"Nat. Commun."},{"key":"2022072708533167300_JCS260015C44","doi-asserted-by":"publisher","first-page":"11874","DOI":"10.1038\/ncomms11874","article-title":"Molecular basis for CPAP-tubulin interaction in controlling centriolar and ciliary length","volume":"7","author":"Zheng","year":"2016","journal-title":"Nat. Commun."}],"container-title":["Journal of Cell Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.biologists.com\/jcs\/article-pdf\/doi\/10.1242\/jcs.260015\/2148678\/jcs260015.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/journals.biologists.com\/jcs\/article-pdf\/doi\/10.1242\/jcs.260015\/2154886\/jcs260015.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/journals.biologists.com\/jcs\/article-pdf\/doi\/10.1242\/jcs.260015\/2154886\/jcs260015.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,7,27]],"date-time":"2022-07-27T04:53:49Z","timestamp":1658897629000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.biologists.com\/jcs\/article\/135\/14\/jcs260015\/276041\/Centriole-distal-end-proteins-CP110-and-Cep97"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,7,15]]},"references-count":43,"journal-issue":{"issue":"14","published-print":{"date-parts":[[2022,7,15]]}},"URL":"https:\/\/doi.org\/10.1242\/jcs.260015","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2021.07.08.451650","asserted-by":"object"}]},"ISSN":["0021-9533","1477-9137"],"issn-type":[{"value":"0021-9533","type":"print"},{"value":"1477-9137","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,7,15]]},"article-number":"jcs260015"}}