{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T02:48:16Z","timestamp":1787021296555,"version":"build-2736575974"},"reference-count":61,"publisher":"The Company of Biologists","issue":"4","license":[{"start":{"date-parts":[[2023,2,15]],"date-time":"2023-02-15T00:00:00Z","timestamp":1676419200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["R01 AI060767"],"award-info":[{"award-number":["R01 AI060767"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["R01 AI134753"],"award-info":[{"award-number":["R01 AI134753"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,2,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Intracellular pathogens exploit cellular resources through host cell manipulation. Within its nonfusogenic parasitophorous vacuole (PV), Toxoplasma gondii targets host nutrient-filled organelles and sequesters them into the PV through deep invaginations of the PV membrane (PVM) that ultimately detach from this membrane. Some of these invaginations are generated by an intravacuolar network (IVN) of parasite-derived tubules attached to the PVM. Here, we examined the usurpation of host ESCRT-III and Vps4A by the parasite to create PVM buds and vesicles. CHMP4B associated with the PVM\/IVN, and dominant-negative (DN) CHMP4B formed many long PVM invaginations containing CHMP4B filaments. These invaginations were shorter in IVN-deficient parasites, suggesting cooperation between the IVN and ESCRT. In infected cells expressing Vps4A-DN, enlarged intra-PV structures containing host endolysosomes accumulated, reflecting defects in PVM scission. Parasite mutants lacking T. gondii (Tg)GRA14 or TgGRA64, which interact with ESCRT, reduced CHMP4B-DN-induced PVM invaginations and intra-PV host organelles, with greater defects in a double knockout, revealing the exploitation of ESCRT to scavenge host organelles by Toxoplasma.<\/jats:p>","DOI":"10.1242\/jcs.260159","type":"journal-article","created":{"date-parts":[[2023,1,31]],"date-time":"2023-01-31T03:47:35Z","timestamp":1675136855000},"source":"Crossref","is-referenced-by-count":13,"title":["<i>Toxoplasma gondii<\/i>\n                    scavenges mammalian host organelles through the usurpation of host ESCRT-III and Vps4A"],"prefix":"10.1242","volume":"136","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8956-7377","authenticated-orcid":false,"given":"Julia D.","family":"Romano","sequence":"first","affiliation":[{"name":"Johns Hopkins University Bloomberg School of Public Health 1 Department of Molecular Microbiology and Immunology , , Baltimore, MD 21205 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0859-2226","authenticated-orcid":false,"given":"Joshua","family":"Mayoral","sequence":"additional","affiliation":[{"name":"Albert Einstein College of Medicine 2 Department of Pathology , , Bronx, NY 10461 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8040-3453","authenticated-orcid":false,"given":"Rebekah B.","family":"Guevara","sequence":"additional","affiliation":[{"name":"Albert Einstein College of Medicine 2 Department of Pathology , , Bronx, NY 10461 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8389-5724","authenticated-orcid":false,"given":"Yolanda","family":"Rivera-Cuevas","sequence":"additional","affiliation":[{"name":"University of Michigan Medical School 3 Department of Microbiology and Immunology , , Ann Arbor, MI 48109 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6859-8895","authenticated-orcid":false,"given":"Vern B.","family":"Carruthers","sequence":"additional","affiliation":[{"name":"University of Michigan Medical School 3 Department of Microbiology and Immunology , , Ann Arbor, MI 48109 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0357-7396","authenticated-orcid":false,"given":"Louis M.","family":"Weiss","sequence":"additional","affiliation":[{"name":"Albert Einstein College of Medicine 2 Department of Pathology , , Bronx, NY 10461 , USA"},{"name":"Albert Einstein College of Medicine 4 Department of Medicine , , Bronx, NY 10461 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5549-2362","authenticated-orcid":false,"given":"Isabelle","family":"Coppens","sequence":"additional","affiliation":[{"name":"Johns Hopkins University Bloomberg School of Public Health 1 Department of Molecular Microbiology and Immunology , , Baltimore, MD 21205 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2023,2,22]]},"reference":[{"key":"2023022210152650100_","doi-asserted-by":"publisher","first-page":"3288","DOI":"10.1111\/febs.13688","article-title":"ESCRT-III and Vps4: a dynamic multipurpose tool for membrane budding and scission","volume":"283","author":"Alonso Y. 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