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In a smaller proximal ciliary region that lacks mastigonemes, PKD2 is more mobile. We show that the PKD2 regions are established early during ciliogenesis and increase proportionally in length as cilia elongate. In chimeric zygotes, tagged PKD2 rapidly entered the proximal region of PKD2-deficient cilia, whereas the assembly of the distal region was hindered, suggesting that axonemal binding of PKD2 requires de novo assembly of cilia. We identified the protein Small Interactor of PKD2 (SIP), a PKD2-related, single-pass transmembrane protein, as part of the PKD2\u2013mastigoneme complex. In sip mutants, stability and proteolytic processing of PKD2 in the cell body were reduced and PKD2\u2013mastigoneme complexes were absent from the cilia. Like the pkd2 and mst1 mutants, sip mutant cells swam with reduced velocity. Cilia of the pkd2 mutant beat with an increased frequency but were less efficient in moving the cells, suggesting a structural role for the PKD2\u2013SIP\u2013mastigoneme complex in increasing the effective surface of Chlamydomonas cilia.<\/jats:p>","DOI":"10.1242\/jcs.261497","type":"journal-article","created":{"date-parts":[[2023,12,8]],"date-time":"2023-12-08T07:34:00Z","timestamp":1702020840000},"source":"Crossref","is-referenced-by-count":13,"title":["The Small Interactor of PKD2 protein promotes the assembly and ciliary entry of the\n                    <i>Chlamydomonas<\/i>\n                    PKD2\u2013mastigoneme complexes"],"prefix":"10.1242","volume":"137","author":[{"given":"Poulomi","family":"Das","sequence":"first","affiliation":[{"name":"University of Georgia 1 Department of Cellular Biology , , Athens, GA 30602 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Betlehem","family":"Mekonnen","sequence":"additional","affiliation":[{"name":"University of Georgia 1 Department of Cellular Biology , , Athens, GA 30602 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rama","family":"Alkhofash","sequence":"additional","affiliation":[{"name":"University of Georgia 1 Department of Cellular Biology , , Athens, GA 30602 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abha V.","family":"Ingle","sequence":"additional","affiliation":[{"name":"University of Georgia 2 Department of Computer Science , , Athens, GA 30602 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E. Blair","family":"Workman","sequence":"additional","affiliation":[{"name":"University of Georgia 1 Department of Cellular Biology , , Athens, GA 30602 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alec","family":"Feather","sequence":"additional","affiliation":[{"name":"University of Georgia 1 Department of Cellular Biology , , Athens, GA 30602 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gui","family":"Zhang","sequence":"additional","affiliation":[{"name":"University of Georgia 1 Department of Cellular Biology , , Athens, GA 30602 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathan","family":"Chasen","sequence":"additional","affiliation":[{"name":"University of Georgia 1 Department of Cellular Biology , , Athens, GA 30602 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5597-2200","authenticated-orcid":false,"given":"Peiwei","family":"Liu","sequence":"additional","affiliation":[{"name":"University of Georgia 1 Department of Cellular Biology , , Athens, GA 30602 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6219-6470","authenticated-orcid":false,"given":"Karl F.","family":"Lechtreck","sequence":"additional","affiliation":[{"name":"University of Georgia 1 Department of Cellular Biology , , Athens, GA 30602 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2024,1,12]]},"reference":[{"key":"2024011216003489900_JCS261497C1","doi-asserted-by":"publisher","first-page":"2914","DOI":"10.1016\/j.bpj.2020.05.003","article-title":"Fibrous flagellar hairs of Chlamydomonas reinhardtii do not enhance swimming","volume":"118","author":"Amador","year":"2020","journal-title":"Biophys. 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