{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T18:10:55Z","timestamp":1784916655473,"version":"3.55.0"},"reference-count":54,"publisher":"The Company of Biologists","issue":"18","license":[{"start":{"date-parts":[[2023,9,20]],"date-time":"2023-09-20T00:00:00Z","timestamp":1695168000000},"content-version":"vor","delay-in-days":5,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"Medical Research Council","award":["2251399"],"award-info":[{"award-number":["2251399"]}]},{"name":"Belgian National Fund for Scientific Research F.R.S. - FNRS"},{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"crossref","award":["210904\/Z\/18\/Z"],"award-info":[{"award-number":["210904\/Z\/18\/Z"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"crossref","award":["205534\/Z\/17\/Z"],"award-info":[{"award-number":["205534\/Z\/17\/Z"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"crossref","award":["220906\/Z\/20\/Z"],"award-info":[{"award-number":["220906\/Z\/20\/Z"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100000735","name":"University of Cambridge","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100000735","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,9,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>Myelinating Schwann cell (SC)\u2013dorsal root ganglion (DRG) neuron cocultures are an important technique for understanding cell\u2013cell signalling and interactions during peripheral nervous system (PNS) myelination, injury, and regeneration. Although methods using rat SCs and neurons or mouse DRG explants are commonplace, there are no established protocols for compartmentalised myelinating cocultures with dissociated mouse cells. There consequently is a need for a coculture protocol that allows separate genetic manipulation of mouse SCs or neurons, or use of cells from different transgenic animals to complement in vivo mouse experiments. However, inducing myelination of dissociated mouse SCs in culture is challenging. Here, we describe a new method to coculture dissociated mouse SCs and DRG neurons in microfluidic chambers and induce robust myelination. Cocultures can be axotomised to study injury and used for drug treatments, and cells can be lentivirally transduced for live imaging. We used this model to investigate axon degeneration after traumatic axotomy and find that SCs, irrespective of myelination status, are axo-protective. At later timepoints after injury, live imaging of cocultures shows that SCs break up, ingest and clear axonal debris.<\/jats:p>","DOI":"10.1242\/jcs.261557","type":"journal-article","created":{"date-parts":[[2023,8,29]],"date-time":"2023-08-29T14:51:48Z","timestamp":1693320708000},"source":"Crossref","is-referenced-by-count":16,"title":["Schwann cells are axo-protective after injury irrespective of myelination status in mouse Schwann cell\u2013neuron cocultures"],"prefix":"10.1242","volume":"136","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2189-2389","authenticated-orcid":false,"given":"Clara","family":"Mutschler","sequence":"first","affiliation":[{"name":"John Van Geest Centre for Brain Repair 1 , Department of Clinical Neurosciences , , Cambridge CB2 0PY , UK"},{"name":"University of Cambridge 1 , Department of Clinical Neurosciences , , Cambridge CB2 0PY , UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaline V.","family":"Fazal","sequence":"additional","affiliation":[{"name":"John Van Geest Centre for Brain Repair 1 , Department of Clinical Neurosciences , , Cambridge CB2 0PY , UK"},{"name":"University of Cambridge 1 , Department of Clinical Neurosciences , , Cambridge CB2 0PY , UK"},{"name":"Wellcome-MRC Cambridge Stem Cell Institute, University of Cambridge 2 , Cambridge CB2 0AW , UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathalie","family":"Schumacher","sequence":"additional","affiliation":[{"name":"Laboratory of Nervous System Disorders and Therapies, GIGA Neurosciences, University of Li\u00e8ge 3 , 4000 Li\u00e8ge , Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Loreto","sequence":"additional","affiliation":[{"name":"John Van Geest Centre for Brain Repair 1 , Department of Clinical Neurosciences , , Cambridge CB2 0PY , UK"},{"name":"University of Cambridge 1 , Department of Clinical Neurosciences , , Cambridge CB2 0PY , UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael P.","family":"Coleman","sequence":"additional","affiliation":[{"name":"John Van Geest Centre for Brain Repair 1 , Department of Clinical Neurosciences , , Cambridge CB2 0PY , UK"},{"name":"University of Cambridge 1 , Department of Clinical Neurosciences , , Cambridge CB2 0PY , UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1239-9392","authenticated-orcid":false,"given":"Peter","family":"Arthur-Farraj","sequence":"additional","affiliation":[{"name":"John Van Geest Centre for Brain Repair 1 , Department of Clinical Neurosciences , , Cambridge CB2 0PY , UK"},{"name":"University of Cambridge 1 , Department of Clinical Neurosciences , , Cambridge CB2 0PY , UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2023,9,20]]},"reference":[{"key":"2024061307503278500_JCS261557C1","doi-asserted-by":"publisher","first-page":"2200","DOI":"10.1007\/s13311-021-01125-3","article-title":"Lessons from injury: how nerve injury studies reveal basic biological mechanisms and therapeutic opportunities for peripheral nerve diseases","volume":"18","author":"Arthur-Farraj","year":"2021","journal-title":"Neurotherapeutics"},{"key":"2024061307503278500_JCS261557C2","doi-asserted-by":"publisher","first-page":"720","DOI":"10.1002\/glia.21144","article-title":"Mouse Schwann cells need both NRG1 and cyclic AMP to myelinate","volume":"59","author":"Arthur-Farraj","year":"2011","journal-title":"Glia"},{"key":"2024061307503278500_JCS261557C3","doi-asserted-by":"publisher","first-page":"633","DOI":"10.1016\/j.neuron.2012.06.021","article-title":"c-Jun reprograms Schwann cells of injured nerves to generate a repair cell essential for regeneration","volume":"75","author":"Arthur-Farraj","year":"2012","journal-title":"Neuron"},{"key":"2024061307503278500_JCS261557C4","doi-asserted-by":"publisher","first-page":"2719","DOI":"10.1016\/j.celrep.2017.08.064","article-title":"Changes in the coding and non-coding transcriptome and DNA methylome that define the Schwann cell repair phenotype after nerve injury","volume":"20","author":"Arthur-Farraj","year":"2017","journal-title":"Cell Rep."},{"key":"2024061307503278500_JCS261557C5","doi-asserted-by":"publisher","first-page":"1215","DOI":"10.1038\/s41593-020-0689-4","article-title":"A glycolytic shift in Schwann cells supports injured axons","volume":"23","author":"Babetto","year":"2020","journal-title":"Nat. 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