{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,4]],"date-time":"2026-04-04T02:07:42Z","timestamp":1775268462394,"version":"3.50.1"},"reference-count":35,"publisher":"The Company of Biologists","issue":"3","license":[{"start":{"date-parts":[[2024,2,1]],"date-time":"2024-02-01T00:00:00Z","timestamp":1706745600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"funder":[{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"crossref","award":["R35 GM118027"],"award-info":[{"award-number":["R35 GM118027"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"crossref","award":["AI 134749"],"award-info":[{"award-number":["AI 134749"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100007923","name":"University of Wisconsin Carbone Cancer Center","doi-asserted-by":"crossref","award":["P30 CA014520"],"award-info":[{"award-number":["P30 CA014520"]}],"id":[{"id":"10.13039\/100007923","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,2,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Neutrophil-directed motility is necessary for host defense, but its dysregulation can also cause collateral tissue damage. Actinopathies are monogenic disorders that affect the actin cytoskeleton and lead to immune dysregulation. Deficiency in ARPC1B, a component of the Arp2\/3 complex, results in vascular neutrophilic inflammation; however, the mechanism remains unclear. Here, we generated human induced pluripotent stem cell (iPSC)-derived neutrophils (denoted iNeutrophils) that are deficient in ARPC1B and show impaired migration and a switch from forming pseudopodia to forming elongated filopodia. We show, using a blood vessel on a chip model, that primary human neutrophils have impaired movement across an endothelium deficient in APRC1B. We also show that the combined deficiency of ARPC1B in iNeutrophils and endothelium results in further reduction in neutrophil migration. Taken together, these results suggest that ARPC1B in endothelium is sufficient to drive neutrophil behavior. Furthermore, the findings provide support for using the iPSC system to understand human neutrophil biology and model disease in a genetically tractable system.<\/jats:p>","DOI":"10.1242\/jcs.261774","type":"journal-article","created":{"date-parts":[[2024,1,15]],"date-time":"2024-01-15T07:20:58Z","timestamp":1705303258000},"source":"Crossref","is-referenced-by-count":10,"title":["Neutrophil motility is regulated by both cell intrinsic and endothelial cell ARPC1B"],"prefix":"10.1242","volume":"137","author":[{"given":"Ashley","family":"Peterson","sequence":"first","affiliation":[{"name":"University of Wisconsin-Madison 1 Department of Medical Microbiology and Immunology , , Madison, WI 53706 , USA"},{"name":"University of Wisconsin-Madison 2 Comparative Biomedical Sciences Graduate Program , , Madison, WI 53706 , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Bennin","sequence":"additional","affiliation":[{"name":"University of Wisconsin-Madison 1 Department of Medical Microbiology and Immunology , , Madison, WI 53706 , USA"},{"name":"University of Wisconsin-Madison 3 Department of Pediatrics , , Madison, WI 53706 , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1896-2705","authenticated-orcid":false,"given":"Michael","family":"Lasarev","sequence":"additional","affiliation":[{"name":"University of Wisconsin-Madison School of Medicine and Public Health 4 Department of Biostatistics and Medical Informatics , , Madison, WI 53706 , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julia","family":"Chini","sequence":"additional","affiliation":[{"name":"Perelman School of Medicine, University of Pennsylvania 5 Department of Pediatrics , , Philadelphia, PA 19104 , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0415-9006","authenticated-orcid":false,"given":"David J.","family":"Beebe","sequence":"additional","affiliation":[{"name":"University of Wisconsin-Madison 6 Department of Biomedical Engineering , , Madison, WI 53706 , USA"},{"name":"University of Wisconsin-Madison 7 Department of Pathology and Laboratory Medicine , , Madison, WI 53706 , USA"},{"name":"Carbone Cancer Center, University of Wisconsin-Madison 8 , Madison, WI 53706 , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7940-6254","authenticated-orcid":false,"given":"Anna","family":"Huttenlocher","sequence":"additional","affiliation":[{"name":"University of Wisconsin-Madison 1 Department of Medical Microbiology and Immunology , , Madison, WI 53706 , USA"},{"name":"University of Wisconsin-Madison 3 Department of Pediatrics , , Madison, WI 53706 , USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","published-online":{"date-parts":[[2024,2,12]]},"reference":[{"key":"2024021413453829900_JCS261774C1","doi-asserted-by":"publisher","first-page":"76","DOI":"10.1038\/ncb3286","article-title":"Isoform diversity in the Arp2\/3 complex determines actin filament dynamics","volume":"18","author":"Abella","year":"2016","journal-title":"Nat. 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