{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,19]],"date-time":"2025-08-19T11:04:46Z","timestamp":1755601486907,"version":"3.41.2"},"reference-count":41,"publisher":"The Company of Biologists","issue":"24","license":[{"start":{"date-parts":[[2024,12,17]],"date-time":"2024-12-17T00:00:00Z","timestamp":1734393600000},"content-version":"vor","delay-in-days":2,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"crossref","award":["RGPIN-2015-06727"],"award-info":[{"award-number":["RGPIN-2015-06727"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100008986","name":"University of Guelph","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100008986","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,12,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>The FLO genes in Saccharomyces cerevisiae are repressed by heterochromatin formation, involving histone deacetylases, transcription factors and non-coding RNAs. Here, we report that mutations in the processivity factor POL30 (PCNA) that show transient derepression at the subtelomeres and the mating-type loci do not derepress FLO loci. However, deletions of the replisome stability factors RRM3 and TOF1 along with pol30 mutations induced flocculation phenotypes. The phenotypes correlated with increased expression of reporter proteins driven by the FLO11 promoter, the frequency of silent to active conversions of FLO11, and reduced expression of the regulatory long non-coding RNAs ICR1 and PWR1. Alterations in the local replication landscape of FLO11 indicate a link between defects in the fork protection complex and the stability of gene silencing. Analyses of these mutants at the subtelomeres and the HML\u03b1 locus showed a similar derepression phenotype and suggest transient instability of both active and silent states of FLO11. We conclude that RRM3 and TOF1 interact differentially with the pol30 mutations to promote transient derepression or complete epigenetic conversions of FLO11. We suggest that the interaction between POL30, RRM3 and TOF1 is essential to maintain epigenetic stability at the studied loci.<\/jats:p>","DOI":"10.1242\/jcs.262006","type":"journal-article","created":{"date-parts":[[2024,11,18]],"date-time":"2024-11-18T08:26:59Z","timestamp":1731918419000},"source":"Crossref","is-referenced-by-count":2,"title":["Mutations in the DNA processivity factor <i>POL30<\/i> predispose the <i>FLO11<\/i> locus to epigenetic instability in <i>S. cerevisiae<\/i>"],"prefix":"10.1242","volume":"137","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1022-1822","authenticated-orcid":false,"given":"Safia Mahabub","family":"Sauty","sequence":"first","affiliation":[{"name":"University of Guelph Department of Molecular and Cellular Biology , , Guelph, Ontario N1G2W1 ,","place":["Canada"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-7887-6934","authenticated-orcid":false,"given":"Ashley","family":"Fisher","sequence":"additional","affiliation":[{"name":"University of Guelph Department of Molecular and Cellular Biology , , Guelph, Ontario N1G2W1 ,","place":["Canada"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew","family":"Dolson","sequence":"additional","affiliation":[{"name":"University of Guelph Department of Molecular and Cellular Biology , , Guelph, Ontario N1G2W1 ,","place":["Canada"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9440-9234","authenticated-orcid":false,"given":"Krassimir","family":"Yankulov","sequence":"additional","affiliation":[{"name":"University of Guelph Department of Molecular and Cellular Biology , , Guelph, Ontario N1G2W1 ,","place":["Canada"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","published-online":{"date-parts":[[2024,12,17]]},"reference":[{"key":"2025021411220186100_JCS262006C1","doi-asserted-by":"publisher","first-page":"3104","DOI":"10.1101\/gad.1478906","article-title":"The S. cerevisiae Rrm3p DNA helicase moves with the replication fork and affects replication of all yeast chromosomes","volume":"20","author":"Azvolinsky","year":"2006","journal-title":"Genes Dev."},{"key":"2025021411220186100_JCS262006C2","doi-asserted-by":"publisher","first-page":"926","DOI":"10.1016\/j.molcel.2020.04.012","article-title":"Cryo-EM structure of the fork protection complex bound to CMG at a replication fork","volume":"78","author":"Bareti\u0107","year":"2020","journal-title":"Mol. 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