{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T02:48:54Z","timestamp":1787021334811,"version":"build-2736575974"},"reference-count":63,"publisher":"The Company of Biologists","issue":"20","license":[{"start":{"date-parts":[[2024,9,9]],"date-time":"2024-09-09T00:00:00Z","timestamp":1725840000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"EMBL Corporate Partnership Programme Fellowship"},{"name":"EMBO Scientific Exchange","award":["10212"],"award-info":[{"award-number":["10212"]}]},{"name":"Department of Biotechnology, India","award":["DBT\/2020\/January\/58"],"award-info":[{"award-number":["DBT\/2020\/January\/58"]}]},{"DOI":"10.13039\/501100005116","name":"Jawaharlal Nehru Centre for Advanced Scientific Research","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100005116","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Science and Engineering Research Board, Department of Science and Technology, Govt. of India","award":["JCB\/2020\/000021"],"award-info":[{"award-number":["JCB\/2020\/000021"]}]},{"DOI":"10.13039\/100013060","name":"European Molecular Biology Laboratory","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100013060","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100000780","name":"European Union","doi-asserted-by":"crossref","award":["101078291"],"award-info":[{"award-number":["101078291"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100000780","name":"European Union","doi-asserted-by":"crossref","award":["EIPOD4"],"award-info":[{"award-number":["EIPOD4"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Sklodowska-Curie Actions","award":["847543"],"award-info":[{"award-number":["847543"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,10,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Candida albicans is the most prevalent fungal pathogen associated with candidemia. Similar to other fungi, the complex life cycle of C. albicans has been challenging to study with high-resolution microscopy due to its small size. Here, we employed ultrastructure expansion microscopy (U-ExM) to directly visualise subcellular structures at high resolution in the yeast and during its transition to hyphal growth. N-hydroxysuccinimide (NHS)-ester pan-labelling in combination with immunofluorescence via snapshots of various mitotic stages provided a comprehensive map of nucleolar and mitochondrial segregation dynamics and enabled the resolution of the inner and outer plaque of spindle pole bodies (SPBs). Analyses of microtubules (MTs) and SPBs suggest that C. albicans displays a side-by-side SPB arrangement with a short mitotic spindle and longer astral MTs (aMTs) at the pre-anaphase stage. Modifications to the established U-ExM protocol enabled the expansion of six other human fungal pathogens, revealing that the side-by-side SPB configuration is a plausibly conserved feature shared by many fungal species. We highlight the power of U-ExM to investigate subcellular organisation at high resolution and low cost in poorly studied and medically relevant microbial pathogens.<\/jats:p>","DOI":"10.1242\/jcs.262046","type":"journal-article","created":{"date-parts":[[2024,7,25]],"date-time":"2024-07-25T08:46:11Z","timestamp":1721897171000},"source":"Crossref","is-referenced-by-count":15,"title":["Expansion microscopy reveals characteristic ultrastructural features of pathogenic budding yeast species"],"prefix":"10.1242","volume":"137","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1370-6892","authenticated-orcid":false,"given":"Md Hashim","family":"Reza","sequence":"first","affiliation":[{"name":"Jawaharlal Nehru Centre for Advanced Scientific Research 1 Molecular Mycology Laboratory, Molecular Biology and Genetics Unit , , Jakkur, Bengaluru 560064 , India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-6583-6593","authenticated-orcid":false,"given":"Srijana","family":"Dutta","sequence":"additional","affiliation":[{"name":"Jawaharlal Nehru Centre for Advanced Scientific Research 1 Molecular Mycology Laboratory, Molecular Biology and Genetics Unit , , Jakkur, Bengaluru 560064 , India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-1831-0306","authenticated-orcid":false,"given":"Rohit","family":"Goyal","sequence":"additional","affiliation":[{"name":"Jawaharlal Nehru Centre for Advanced Scientific Research 1 Molecular Mycology Laboratory, Molecular Biology and Genetics Unit , , Jakkur, Bengaluru 560064 , India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8143-8244","authenticated-orcid":false,"given":"Hiral","family":"Shah","sequence":"additional","affiliation":[{"name":"Cell Biology and Biophysics, European Molecular Biology Laboratory 2 , Heidelberg 69117 , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1416-6223","authenticated-orcid":false,"given":"Gautam","family":"Dey","sequence":"additional","affiliation":[{"name":"Cell Biology and Biophysics, European Molecular Biology Laboratory 2 , Heidelberg 69117 , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6611-4073","authenticated-orcid":false,"given":"Kaustuv","family":"Sanyal","sequence":"additional","affiliation":[{"name":"Jawaharlal Nehru Centre for Advanced Scientific Research 1 Molecular Mycology Laboratory, Molecular Biology and Genetics Unit , , Jakkur, Bengaluru 560064 , India"},{"name":"Bose Institute, Unified Academic Campus 3 Department of Biological Sciences , , EN-80, Sector V, Bidhan Nagar, Kolkata 700091 , India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2024,9,9]]},"reference":[{"key":"2024090908421734600_JCS262046C1","doi-asserted-by":"publisher","first-page":"6532","DOI":"10.1074\/jbc.M207039200","article-title":"The signal sequence of exported protein-1 directs the green fluorescent protein to the Parasitophorous vacuole of transfected Malaria parasites","volume":"278","author":"Adisa","year":"2003","journal-title":"J. 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