{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T00:32:47Z","timestamp":1792801967529,"version":"4.5.0"},"reference-count":64,"publisher":"The Company of Biologists","issue":"1","license":[{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"crossref","award":["22K06637"],"award-info":[{"award-number":["22K06637"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100007591","name":"Akiyama Life Science Foundation","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100007591","id-type":"DOI","asserted-by":"crossref"}]},{"name":"The Hokkoku Cancer Foundation","id":[{"id":"https:\/\/ror.org\/009yhf078","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,1,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>FYVE-type zinc finger-containing phosphoinositide kinase (PIKFYVE), which is essential for phosphatidylinositol 3,5-bisphosphate [PtdIns(3,5)P2] production, is an important regulator of lysosomal homeostasis. PIKFYVE dysfunction leads to cytoplasmic vacuolization; however, the underlying mechanism remains unknown. In this study, we explored the cause of vacuole enlargement upon PIKFYVE inhibition in DU145 prostate cancer cells. Enlargement of vacuoles upon PIKFYVE inhibition required glutamine and its metabolism by glutaminases. Addition of ammonia, a metabolite of glutamine, was sufficient to enlarge vacuoles via PIKFYVE inhibition. Moreover, PIKFYVE inhibition led to intracellular ammonium accumulation. Endosome\u2013lysosome permeabilization resulted in ammonium leakage from the cells, indicating ammonium accumulation in the endosomes and lysosomes. Ammonium accumulation and vacuole expansion were suppressed by the lysosomal lumen neutralization. It is therefore assumed that PIKFYVE inhibition interferes with the efflux of NH4+, which formed through protonation of NH3 in the lysosomal lumen, leading to osmotic swelling of vacuoles. Notably, glutamine or ammonium is required for PIKFYVE inhibition-induced suppression of lysosomal function and autophagic flux. In conclusion, this study shows that PIKFYVE inhibition disrupts lysosomal homeostasis via ammonium accumulation.<\/jats:p>","DOI":"10.1242\/jcs.262236","type":"journal-article","created":{"date-parts":[[2024,11,26]],"date-time":"2024-11-26T04:42:06Z","timestamp":1732596126000},"source":"Crossref","is-referenced-by-count":22,"title":["PIKFYVE inhibition induces endosome- and lysosome-derived vacuole enlargement via ammonium accumulation"],"prefix":"10.1242","volume":"138","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6123-9061","authenticated-orcid":false,"given":"Junsuke","family":"Uwada","sequence":"first","affiliation":[{"name":"School of Medicine, Kanazawa Medical University 1 Department of Pharmacology , , Ishikawa 920-0293 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hitomi","family":"Nakazawa","sequence":"additional","affiliation":[{"name":"School of Medicine, Kanazawa Medical University 1 Department of Pharmacology , , Ishikawa 920-0293 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0842-1614","authenticated-orcid":false,"given":"Takeshi","family":"Kiyoi","sequence":"additional","affiliation":[{"name":"School of Medicine, Kanazawa Medical University 1 Department of Pharmacology , , Ishikawa 920-0293 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4526-6585","authenticated-orcid":false,"given":"Takashi","family":"Yazawa","sequence":"additional","affiliation":[{"name":"Asahikawa Medical University 2 Department of Biochemistry , , Asahikawa 078-8510 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6881-5391","authenticated-orcid":false,"given":"Ikunobu","family":"Muramatsu","sequence":"additional","affiliation":[{"name":"School of Medicine, Kanazawa Medical University 1 Department of Pharmacology , , Ishikawa 920-0293 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1689-8762","authenticated-orcid":false,"given":"Takayoshi","family":"Masuoka","sequence":"additional","affiliation":[{"name":"School of Medicine, Kanazawa Medical University 1 Department of Pharmacology , , Ishikawa 920-0293 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2025,1,10]]},"reference":[{"key":"2025011013211300700_JCS262236C1","doi-asserted-by":"publisher","first-page":"101097","DOI":"10.1016\/j.gim.2024.101097","article-title":"Biallelic loss-of-function variants of SLC12A9 cause lysosome dysfunction and a syndromic neurodevelopmental disorder","volume":"26","author":"Accogli","year":"2024","journal-title":"Genet. Med."},{"key":"2025011013211300700_JCS262236C2","doi-asserted-by":"publisher","first-page":"619","DOI":"10.1038\/nrc.2016.71","article-title":"From Krebs to clinic: glutamine metabolism to cancer therapy","volume":"16","author":"Altman","year":"2016","journal-title":"Nat. Rev. Cancer"},{"key":"2025011013211300700_JCS262236C3","doi-asserted-by":"publisher","first-page":"2424","DOI":"10.1093\/bioinformatics\/btx180","article-title":"Trainable Weka Segmentation: a machine learning tool for microscopy pixel classification","volume":"33","author":"Arganda-Carreras","year":"2017","journal-title":"Bioinformatics"},{"key":"2025011013211300700_JCS262236C4","doi-asserted-by":"publisher","first-page":"747","DOI":"10.1111\/tra.12525","article-title":"PIKfyve activity regulates reformation of terminal storage lysosomes from endolysosomes","volume":"18","author":"Bissig","year":"2017","journal-title":"Traffic"},{"key":"2025011013211300700_JCS262236C5","doi-asserted-by":"publisher","first-page":"595","DOI":"10.1007\/s10545-012-9546-2","article-title":"Ammonia toxicity to the brain","volume":"36","author":"Braissant","year":"2013","journal-title":"J. Inherit. Metab. Dis."},{"key":"2025011013211300700_JCS262236C6","doi-asserted-by":"publisher","first-page":"357","DOI":"10.1038\/s41573-022-00582-5","article-title":"Beyond PI3Ks: targeting phosphoinositide kinases in disease","volume":"22","author":"Burke","year":"2023","journal-title":"Nat. Rev. Drug Discov."},{"key":"2025011013211300700_JCS262236C7","doi-asserted-by":"publisher","first-page":"879","DOI":"10.1083\/jcb.201409071","article-title":"Calcium release through P2X4 activates calmodulin to promote endolysosomal membrane fusion","volume":"209","author":"Cao","year":"2015","journal-title":"J. Cell Biol."},{"key":"2025011013211300700_JCS262236C8","doi-asserted-by":"publisher","first-page":"e1010800","DOI":"10.1371\/journal.pgen.1010800","article-title":"The chloride antiporter CLCN7 is a modifier of lysosome dysfunction in FIG4 and VAC14 mutants","volume":"19","author":"Cao","year":"2023","journal-title":"PLoS Genet."},{"key":"2025011013211300700_JCS262236C9","doi-asserted-by":"publisher","first-page":"68","DOI":"10.1038\/nature05876","article-title":"Mutation of FIG4 causes neurodegeneration in the pale tremor mouse and patients with CMT4J","volume":"448","author":"Chow","year":"2007","journal-title":"Nature"},{"key":"2025011013211300700_JCS262236C10","doi-asserted-by":"publisher","first-page":"jcs213587","DOI":"10.1242\/jcs.213587","article-title":"Lysosome enlargement during inhibition of the lipid kinase PIKfyve proceeds through lysosome coalescence","volume":"131","author":"Choy","year":"2018","journal-title":"J. Cell Sci."},{"key":"2025011013211300700_JCS262236C11","doi-asserted-by":"publisher","first-page":"7707","DOI":"10.1074\/jbc.272.12.7707","article-title":"Calmodulin regulates endosome fusion","volume":"272","author":"Colombo","year":"1997","journal-title":"J. Biol. Chem."},{"key":"2025011013211300700_JCS262236C12","doi-asserted-by":"publisher","first-page":"38","DOI":"10.1038\/ncomms1037","article-title":"PI(3,5)P(2) controls membrane trafficking by direct activation of mucolipin Ca(2+) release channels in the endolysosome","volume":"1","author":"Dong","year":"2010","journal-title":"Nat. Commun."},{"key":"2025011013211300700_JCS262236C13","doi-asserted-by":"publisher","first-page":"dmm047134","DOI":"10.1242\/dmm.047134","article-title":"Ammonia inhibits energy metabolism in astrocytes in a rapid and glutamate dehydrogenase 2-dependent manner","volume":"13","author":"Drews","year":"2020","journal-title":"Dis. Model. Mech."},{"key":"2025011013211300700_JCS262236C14","doi-asserted-by":"publisher","first-page":"119243","DOI":"10.1016\/j.bbamcr.2022.119243","article-title":"The role of lysosomal cathepsins in neurodegeneration: Mechanistic insights, diagnostic potential and therapeutic approaches","volume":"1869","author":"Drobny","year":"2022","journal-title":"Biochim. Biophys. Acta Mol. Cell Res."},{"key":"2025011013211300700_JCS262236C15","doi-asserted-by":"publisher","first-page":"49","DOI":"10.1247\/csf.17003","article-title":"PI5P and PI(3,5)P(2): minor, but essential phosphoinositides","volume":"42","author":"Hasegawa","year":"2017","journal-title":"Cell Struct. Funct."},{"key":"2025011013211300700_JCS262236C16","doi-asserted-by":"publisher","first-page":"9206","DOI":"10.1074\/jbc.M108750200","article-title":"Functional dissection of lipid and protein kinase signals of PIKfyve reveals the role of PtdIns 3,5-P2 production for endomembrane integrity","volume":"277","author":"Ikonomov","year":"2002","journal-title":"J. Biol. Chem."},{"key":"2025011013211300700_JCS262236C17","doi-asserted-by":"publisher","first-page":"26760","DOI":"10.1074\/jbc.C110.154658","article-title":"ArPIKfyve regulates Sac3 protein abundance and turnover: disruption of the mechanism by Sac3I41T mutation causing Charcot-Marie-Tooth 4J disorder","volume":"285","author":"Ikonomov","year":"2010","journal-title":"J. Biol. Chem."},{"key":"2025011013211300700_JCS262236C18","doi-asserted-by":"publisher","first-page":"13404","DOI":"10.1074\/jbc.M111.222364","article-title":"The phosphoinositide kinase PIKfyve is vital in early embryonic development: preimplantation lethality of PIKfyve-\/- embryos but normality of PIKfyve+\/- mice","volume":"286","author":"Ikonomov","year":"2011","journal-title":"J. Biol. Chem."},{"key":"2025011013211300700_JCS262236C19","doi-asserted-by":"publisher","first-page":"1017","DOI":"10.1101\/pdb.prot086165","article-title":"Quantification of lysosomal membrane permeabilization by cytosolic cathepsin and beta-N-Acetyl-glucosaminidase activity measurements","volume":"2015","author":"Jaattela","year":"2015","journal-title":"Cold Spring Harb. Protoc."},{"key":"2025011013211300700_JCS262236C20","doi-asserted-by":"publisher","first-page":"706","DOI":"10.1038\/s12276-023-00971-9","article-title":"Targeting glutamine metabolism as a therapeutic strategy for cancer","volume":"55","author":"Jin","year":"2023","journal-title":"Exp. Mol. Med."},{"key":"2025011013211300700_JCS262236C21","doi-asserted-by":"publisher","first-page":"135","DOI":"10.2147\/RRU.S173377","article-title":"Caveolin-1 and \u22122 regulate cell motility in castration-resistant prostate cancer","volume":"10","author":"Kamibeppu","year":"2018","journal-title":"Res. Rep. Urol."},{"key":"2025011013211300700_JCS262236C22","doi-asserted-by":"publisher","first-page":"154","DOI":"10.4161\/auto.3634","article-title":"Autophagosome-lysosome fusion depends on the pH in acidic compartments in CHO cells","volume":"3","author":"Kawai","year":"2007","journal-title":"Autophagy"},{"key":"2025011013211300700_JCS262236C23","first-page":"2954","article-title":"A novel mutation (p.Glu1389AspfsX16) of the phosphoinositide kinase, FYVE finger containing gene found in a Japanese patient with fleck corneal dystrophy","volume":"18","author":"Kawasaki","year":"2012","journal-title":"Mol. Vis."},{"key":"2025011013211300700_JCS262236C24","doi-asserted-by":"publisher","first-page":"3640","DOI":"10.1016\/j.febslet.2015.10.029","article-title":"Activation of muscarinic cholinoceptor ameliorates tumor necrosis factor-alpha-induced barrier dysfunction in intestinal epithelial cells","volume":"589","author":"Khan","year":"2015","journal-title":"FEBS Lett."},{"key":"2025011013211300700_JCS262236C25","doi-asserted-by":"publisher","first-page":"1902","DOI":"10.3390\/cells9081902","article-title":"Highlights on genomics applications for lysosomal storage diseases","volume":"9","author":"La Cognata","year":"2020","journal-title":"Cells"},{"key":"2025011013211300700_JCS262236C26","doi-asserted-by":"publisher","first-page":"e14714","DOI":"10.15252\/emmm.202114714","article-title":"Loss of PIKfyve drives the spongiform degeneration in prion diseases","volume":"13","author":"Lakkaraju","year":"2021","journal-title":"EMBO Mol. Med."},{"key":"2025011013211300700_JCS262236C27","doi-asserted-by":"publisher","first-page":"1835","DOI":"10.3390\/cells12141835","article-title":"Modulation of Lysosomal Cl(-) mediates migration and apoptosis through the TRPML1 as a Lysosomal Cl(-) sensor","volume":"12","author":"Lee","year":"2023","journal-title":"Cells"},{"key":"2025011013211300700_JCS262236C28","doi-asserted-by":"publisher","first-page":"e74136","DOI":"10.7554\/eLife.74136","article-title":"Tonic inhibition of the chloride\/proton antiporter ClC-7 by PI(3,5)P2 is crucial for lysosomal pH maintenance","volume":"11","author":"Leray","year":"2022","journal-title":"eLife"},{"key":"2025011013211300700_JCS262236C29","doi-asserted-by":"publisher","first-page":"S1534","DOI":"10.1016\/j.devcel.2024.10.003","article-title":"An SLC12A9-dependent ion transport mechanism maintains lysosomal osmolarity","volume":"21","author":"Levin-Konigsberg","year":"2024","journal-title":"Dev. Cell"},{"key":"2025011013211300700_JCS262236C30","doi-asserted-by":"publisher","first-page":"54","DOI":"10.1086\/431346","article-title":"Mutations in PIP5K3 are associated with Francois-Neetens mouchetee fleck corneal dystrophy","volume":"77","author":"Li","year":"2005","journal-title":"Am. J. Hum. Genet."},{"key":"2025011013211300700_JCS262236C31","doi-asserted-by":"publisher","first-page":"76","DOI":"10.1016\/j.celrep.2019.08.076","article-title":"Liver-type glutaminase GLS2 is a druggable metabolic node in luminal-subtype breast cancer","volume":"29","author":"Lukey","year":"2019","journal-title":"Cell Rep."},{"key":"2025011013211300700_JCS262236C32","doi-asserted-by":"publisher","first-page":"e60152","DOI":"10.1371\/journal.pone.0060152","article-title":"Inhibition of PIKfyve by YM-201636 dysregulates autophagy and leads to apoptosis-independent neuronal cell death","volume":"8","author":"Martin","year":"2013","journal-title":"PLoS ONE"},{"key":"2025011013211300700_JCS262236C33","doi-asserted-by":"publisher","first-page":"e23054","DOI":"10.1371\/journal.pone.0023054","article-title":"SB202190-induced cell type-specific vacuole formation and defective autophagy do not depend on p38 MAP kinase inhibition","volume":"6","author":"Menon","year":"2011","journal-title":"PLoS ONE"},{"key":"2025011013211300700_JCS262236C34","doi-asserted-by":"publisher","first-page":"1425","DOI":"10.1080\/15548627.2015.1059562","article-title":"The problem of pyridinyl imidazole class inhibitors of MAPK14\/p38alpha and MAPK11\/p38beta in autophagy research","volume":"11","author":"Menon","year":"2015","journal-title":"Autophagy"},{"key":"2025011013211300700_JCS262236C35","doi-asserted-by":"publisher","first-page":"1477","DOI":"10.1084\/jem.20072194","article-title":"Membrane traffic and turnover in TRP-ML1-deficient cells: a revised model for mucolipidosis type IV pathogenesis","volume":"205","author":"Miedel","year":"2008","journal-title":"J. Exp. Med."},{"key":"2025011013211300700_JCS262236C63","doi-asserted-by":"publisher","first-page":"jcs261765","DOI":"10.1242\/jcs.261765","article-title":"Collapse of late endosomal pH elicits a rapid Rab7 response via the V-ATPase and RILP","volume":"137","author":"Mulligan","year":"2024","journal-title":"J. Cell Sci."},{"key":"2025011013211300700_JCS262236C36","doi-asserted-by":"publisher","first-page":"638","DOI":"10.3892\/ol.2021.12899","article-title":"Restoration of the ATG5-dependent autophagy sensitizes DU145 prostate cancer cells to chemotherapeutic drugs","volume":"22","author":"Peng","year":"2021","journal-title":"Oncol. Lett."},{"key":"2025011013211300700_JCS262236C37","doi-asserted-by":"publisher","first-page":"665","DOI":"10.1083\/jcb.90.3.665","article-title":"Effect of weak bases on the intralysosomal pH in mouse peritoneal macrophages","volume":"90","author":"Poole","year":"1981","journal-title":"J. Cell Biol."},{"key":"2025011013211300700_JCS262236C38","doi-asserted-by":"publisher","first-page":"1053","DOI":"10.1083\/jcb.149.5.1053","article-title":"The role of intraorganellar Ca(2+) in late endosome-lysosome heterotypic fusion and in the reformation of lysosomes from hybrid organelles","volume":"149","author":"Pryor","year":"2000","journal-title":"J. Cell Biol."},{"key":"2025011013211300700_JCS262236C39","doi-asserted-by":"publisher","first-page":"e1004833","DOI":"10.1371\/journal.pgen.1004833","article-title":"Mucolipin co-deficiency causes accelerated endolysosomal vacuolation of enterocytes and failure-to-thrive from birth to weaning","volume":"10","author":"Remis","year":"2014","journal-title":"PLoS Genet."},{"key":"2025011013211300700_JCS262236C40","doi-asserted-by":"publisher","first-page":"139","DOI":"10.15252\/embj.201695709","article-title":"Glyoxal as an alternative fixative to formaldehyde in immunostaining and super-resolution microscopy","volume":"37","author":"Richter","year":"2018","journal-title":"EMBO J."},{"key":"2025011013211300700_JCS262236C41","doi-asserted-by":"publisher","first-page":"1576","DOI":"10.1002\/1873-3468.12195","article-title":"Vacuolin-1 inhibits autophagy by impairing lysosomal maturation via PIKfyve inhibition","volume":"590","author":"Sano","year":"2016","journal-title":"FEBS Lett."},{"key":"2025011013211300700_JCS262236C42","doi-asserted-by":"publisher","first-page":"749","DOI":"10.1016\/j.bbamcr.2017.02.012","article-title":"Cellular vacuolization caused by overexpression of the PIKfyve-binding deficient Vac14(L156R) is rescued by starvation and inhibition of vacuolar-ATPase","volume":"1864","author":"Schulze","year":"2017","journal-title":"Biochim. Biophys. Acta Mol. Cell Res."},{"key":"2025011013211300700_JCS262236C43","doi-asserted-by":"publisher","first-page":"283","DOI":"10.1038\/nrm3565","article-title":"Signals from the lysosome: a control centre for cellular clearance and energy metabolism","volume":"14","author":"Settembre","year":"2013","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"2025011013211300700_JCS262236C44","doi-asserted-by":"publisher","first-page":"1694","DOI":"10.1080\/15548627.2019.1586257","article-title":"A family of PIKFYVE inhibitors with therapeutic potential against autophagy-dependent cancer cells disrupt multiple events in lysosome homeostasis","volume":"15","author":"Sharma","year":"2019","journal-title":"Autophagy"},{"key":"2025011013211300700_JCS262236C45","doi-asserted-by":"publisher","first-page":"7294","DOI":"10.1074\/jbc.M508211200","article-title":"TRP-ML1 regulates lysosomal pH and acidic lysosomal lipid hydrolytic activity","volume":"281","author":"Soyombo","year":"2006","journal-title":"J. Biol. Chem."},{"key":"2025011013211300700_JCS262236C46","doi-asserted-by":"publisher","first-page":"10304","DOI":"10.1038\/s41598-017-09993-6","article-title":"An LC-MS approach to quantitative measurement of ammonia isotopologues","volume":"7","author":"Spinelli","year":"2017","journal-title":"Sci. Rep."},{"key":"2025011013211300700_JCS262236C47","doi-asserted-by":"publisher","first-page":"87","DOI":"10.1186\/s12929-020-00679-2","article-title":"Amino acid-dependent control of mTORC1 signaling: a variety of regulatory modes","volume":"27","author":"Takahara","year":"2020","journal-title":"J. Biomed. Sci."},{"key":"2025011013211300700_JCS262236C48","doi-asserted-by":"publisher","first-page":"1726","DOI":"10.1073\/pnas.1213212110","article-title":"Critical roles of type III phosphatidylinositol phosphate kinase in murine embryonic visceral endoderm and adult intestine","volume":"110","author":"Takasuga","year":"2013","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2025011013211300700_JCS262236C49","doi-asserted-by":"publisher","first-page":"154","DOI":"10.1111\/tra.12346","article-title":"Phosphatidylinositol 3,5-bisphosphate-rich membrane domains in endosomes and lysosomes","volume":"17","author":"Takatori","year":"2016","journal-title":"Traffic"},{"key":"2025011013211300700_JCS262236C50","doi-asserted-by":"publisher","first-page":"114297","DOI":"10.1016\/j.bcp.2020.114297","article-title":"PNU-120596, a positive allosteric modulator of alpha7 nicotinic acetylcholine receptor, directly inhibits p38 MAPK","volume":"182","author":"Uwada","year":"2020","journal-title":"Biochem. Pharmacol."},{"key":"2025011013211300700_JCS262236C51","doi-asserted-by":"publisher","first-page":"119554","DOI":"10.1016\/j.lfs.2021.119554","article-title":"Pleiotropic effects of probenecid on three-dimensional cultures of prostate cancer cells","volume":"278","author":"Uwada","year":"2021","journal-title":"Life Sci."},{"key":"2025011013211300700_JCS262236C52","doi-asserted-by":"publisher","first-page":"48","DOI":"10.1016\/j.ceca.2014.10.008","article-title":"The role of TRPMLs in endolysosomal trafficking and function","volume":"58","author":"Venkatachalam","year":"2015","journal-title":"Cell Calcium"},{"key":"2025011013211300700_JCS262236C53","doi-asserted-by":"publisher","first-page":"1070","DOI":"10.1086\/521954","article-title":"Neurologic, gastric, and opthalmologic pathologies in a murine model of mucolipidosis type IV","volume":"81","author":"Venugopal","year":"2007","journal-title":"Am. J. Hum. Genet."},{"key":"2025011013211300700_JCS262236C64","doi-asserted-by":"publisher","first-page":"206","DOI":"10.1016\/j.ymgme.2011.06.006","article-title":"Mucolipidosis type IV: an update","volume":"104","author":"Wakabayashi","year":"2011","journal-title":"Mol. Genet. Metab."},{"key":"2025011013211300700_JCS262236C54","doi-asserted-by":"publisher","first-page":"4056","DOI":"10.1096\/fj.09-130880","article-title":"Lysosomal degradation of endocytosed proteins depends on the chloride transport protein ClC-7","volume":"23","author":"Wartosch","year":"2009","journal-title":"FASEB J."},{"key":"2025011013211300700_JCS262236C55","doi-asserted-by":"publisher","first-page":"80","DOI":"10.1038\/s41419-024-06423-0","article-title":"Unexpected inhibition of the lipid kinase PIKfyve reveals an epistatic role for p38 MAPKs in endolysosomal fission and volume control","volume":"15","author":"Wible","year":"2024","journal-title":"Cell Death Dis."},{"key":"2025011013211300700_JCS262236C56","doi-asserted-by":"publisher","first-page":"3786","DOI":"10.1111\/febs.17059","article-title":"Biological ammonium transporters: evolution and diversification","volume":"291","author":"Williamson","year":"2024","journal-title":"FEBS J."},{"key":"2025011013211300700_JCS262236C57","doi-asserted-by":"publisher","first-page":"383","DOI":"10.1111\/j.1432-1033.1992.tb17061.x","article-title":"Inhibition of the acidification of endosomes and lysosomes by the antibiotic concanamycin B in macrophage J774","volume":"207","author":"Woo","year":"1992","journal-title":"Eur. J. Biochem."},{"key":"2025011013211300700_JCS262236C58","doi-asserted-by":"publisher","first-page":"33","DOI":"10.1247\/csf.23.33","article-title":"Bafilomycin A1 prevents maturation of autophagic vacuoles by inhibiting fusion between autophagosomes and lysosomes in rat hepatoma cell line, H-4-II-E cells","volume":"23","author":"Yamamoto","year":"1998","journal-title":"Cell Struct. Funct."},{"key":"2025011013211300700_JCS262236C59","doi-asserted-by":"publisher","first-page":"e20210200","DOI":"10.1083\/jcb.202102001","article-title":"Lysosome biogenesis: regulation and functions","volume":"220","author":"Yang","year":"2021","journal-title":"J. Cell Biol."},{"key":"2025011013211300700_JCS262236C60","doi-asserted-by":"publisher","first-page":"674","DOI":"10.1111\/tra.12679","article-title":"Biogenesis of lysosome-related organelles complex-1 (BORC) regulates late endosomal\/lysosomal size through PIKfyve-dependent phosphatidylinositol-3,5-bisphosphate","volume":"20","author":"Yordanov","year":"2019","journal-title":"Traffic"},{"key":"2025011013211300700_JCS262236C61","doi-asserted-by":"publisher","first-page":"17518","DOI":"10.1073\/pnas.0702275104","article-title":"Loss of Vac14, a regulator of the signaling lipid phosphatidylinositol 3,5-bisphosphate, results in neurodegeneration in mice","volume":"104","author":"Zhang","year":"2007","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2025011013211300700_JCS262236C62","doi-asserted-by":"publisher","first-page":"17472","DOI":"10.1073\/pnas.1203106109","article-title":"In vivo, Pikfyve generates PI(3,5)P2, which serves as both a signaling lipid and the major precursor for PI5P","volume":"109","author":"Zolov","year":"2012","journal-title":"Proc. Natl. Acad. Sci. USA"}],"container-title":["Journal of Cell Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.biologists.com\/jcs\/article-pdf\/doi\/10.1242\/jcs.262236\/3584091\/jcs262236.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/journals.biologists.com\/jcs\/article-pdf\/doi\/10.1242\/jcs.262236\/3610328\/jcs262236.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/journals.biologists.com\/jcs\/article-pdf\/doi\/10.1242\/jcs.262236\/3610328\/jcs262236.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,10]],"date-time":"2025-01-10T08:21:45Z","timestamp":1736497305000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.biologists.com\/jcs\/article\/138\/1\/jcs262236\/364977\/PIKFYVE-inhibition-induces-endosome-and-lysosome"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1,1]]},"references-count":64,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2025,1,1]]}},"URL":"https:\/\/doi.org\/10.1242\/jcs.262236","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2024.04.19.590364","asserted-by":"object"}]},"ISSN":["0021-9533","1477-9137"],"issn-type":[{"value":"0021-9533","type":"print"},{"value":"1477-9137","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,1,1]]},"article-number":"jcs262236"}}