{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T22:02:07Z","timestamp":1781820127221,"version":"3.54.5"},"reference-count":45,"publisher":"The Company of Biologists","issue":"24","license":[{"start":{"date-parts":[[2024,12,15]],"date-time":"2024-12-15T00:00:00Z","timestamp":1734220800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["R01EY034450"],"award-info":[{"award-number":["R01EY034450"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["P30EY5722"],"award-info":[{"award-number":["P30EY5722"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["R01EY018590"],"award-info":[{"award-number":["R01EY018590"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100001818","name":"Research to Prevent Blindness","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100001818","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,12,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>The transparent ocular lens is essential for vision because it focuses light onto the retina. Despite recognition of the importance of its unique cellular architecture and mechanical properties, the molecular mechanisms governing these attributes remain elusive. This study aims to elucidate the role of ankyrin-B (AnkB, encoded by ANK2), a membrane scaffolding protein, in lens cytoarchitecture, growth and function using a conditional knockout (cKO) mouse model. The AnkB cKO mouse has no defects in lens morphogenesis but exhibited changes that supported a global role for AnkB in maintenance of lens clarity, size, cytoarchitecture, membrane organization and stiffness. Notably, absence of AnkB led to nuclear cataract formation, which was evident from postnatal day 16. AnkB cKO lens fibers exhibit progressive disruption in membrane organization of the spectrin-actin cytoskeleton, cell adhesion proteins and channel proteins; loss and degradation of several membrane proteins [such as NrCAM. N-cadherin (CDH2) and aquaporin-0 (also known as MIP)]; along with a disorganized plasma membrane and impaired membrane interdigitations. Furthermore, absence of AnkB led to decreased lens stiffness. Collectively, these results illustrate the essential role for AnkB in lens architecture, growth and function through its involvement in membrane skeletal and protein organization and stability.<\/jats:p>","DOI":"10.1242\/jcs.262349","type":"journal-article","created":{"date-parts":[[2024,11,19]],"date-time":"2024-11-19T03:22:38Z","timestamp":1731986558000},"source":"Crossref","is-referenced-by-count":5,"title":["Ankyrin-B is required for the establishment and maintenance of lens cytoarchitecture, mechanics and clarity"],"prefix":"10.1242","volume":"137","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1341-3934","authenticated-orcid":false,"given":"Rupalatha","family":"Maddala","sequence":"first","affiliation":[{"name":"Duke University School of Medicine 1 Department of Ophthalmology , , Durham, NC 27710 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ariana","family":"Allen","sequence":"additional","affiliation":[{"name":"Duke University School of Medicine 1 Department of Ophthalmology , , Durham, NC 27710 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3606-0462","authenticated-orcid":false,"given":"Nikolai P.","family":"Skiba","sequence":"additional","affiliation":[{"name":"Duke University School of Medicine 1 Department of Ophthalmology , , Durham, NC 27710 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1553-1691","authenticated-orcid":false,"given":"Ponugoti Vasantha","family":"Rao","sequence":"additional","affiliation":[{"name":"Duke University School of Medicine 1 Department of Ophthalmology , , Durham, NC 27710 ,","place":["USA"]},{"name":"Duke University School of Medicine 2 Department of Pharmacology and Cancer Biology , , Durham, NC 27710 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2024,12,18]]},"reference":[{"key":"2024121810431618200_JCS262349C1","doi-asserted-by":"publisher","first-page":"2701","DOI":"10.1101\/gad.184000","article-title":"Pax6 activity in the lens primordium is required for lens formation and for correct placement of a single retina in the eye","volume":"14","author":"Ashery-Padan","year":"2000","journal-title":"Genes Dev."},{"key":"2024121810431618200_JCS262349C2","doi-asserted-by":"publisher","first-page":"1189","DOI":"10.1016\/j.cell.2008.10.018","article-title":"An ankyrin-based mechanism for functional organization of dystrophin and dystroglycan","volume":"135","author":"Ayalon","year":"2008","journal-title":"Cell"},{"key":"2024121810431618200_JCS262349C3","first-page":"1678","article-title":"Fiber cell denucleation in the primate lens","volume":"38","author":"Bassnett","year":"1997","journal-title":"Invest. 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