{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T14:55:17Z","timestamp":1778770517709,"version":"3.51.4"},"reference-count":88,"publisher":"The Company of Biologists","issue":"9","license":[{"start":{"date-parts":[[2025,4,3]],"date-time":"2025-04-03T00:00:00Z","timestamp":1743638400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"funder":[{"DOI":"10.13039\/501100001843","name":"Science and Engineering Research Board","doi-asserted-by":"crossref","award":["CRG\/2020\/004986"],"award-info":[{"award-number":["CRG\/2020\/004986"]}],"id":[{"id":"10.13039\/501100001843","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001412","name":"Council of Scientific and Industrial Research, India","doi-asserted-by":"crossref","award":["09\/382(0264)\/2020-EMR-I"],"award-info":[{"award-number":["09\/382(0264)\/2020-EMR-I"]}],"id":[{"id":"10.13039\/501100001412","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,5,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Cryptococcus neoformans is a common fungal pathogen, causing fatal meningoencephalitis in immunocompromised individuals. The limited availability of antifungals and increasing resistance in pathogens including C. neoformans emphasize the need to find new drugs. Mitochondria have long been associated with drug resistance in fungi. They are connected to the endoplasmic reticulum (ER) via a multiprotein complex, the ER\u2013mitochondria encounter structure (ERMES), which is unique in the fungal kingdom. In this study on C. neoformans, the four subunits of the ERMES complex, namely, Mmm1, Mdm12, Mdm10 and Mdm34, were deleted to generate the strains \u0394mmm1, \u0394mdm12, \u0394mdm10 and \u0394mdm34, respectively. These mutants had impaired mitochondria and were sensitive to antifungals, including echinocandins, due to lower chitin content. Virulence factors, including capsule formation and melanin production, were debilitated in the mutants. The partner organelle ER was also affected by compromised ERMES contact, as the activity of several ER-synthesized enzymes involved in virulence was impacted. The in vivo studies in Caenorhabditis elegans model of cryptococcosis confirmed the reduced virulence of the mutants. These results indicate that the impairment of the ERMES complex is crucial for the virulence and pathogenesis of C. neoformans.<\/jats:p>","DOI":"10.1242\/jcs.263558","type":"journal-article","created":{"date-parts":[[2025,4,3]],"date-time":"2025-04-03T06:19:00Z","timestamp":1743661140000},"source":"Crossref","is-referenced-by-count":3,"title":["ER\u2013mitochondria encounter structure connections determine drug sensitivity and virulence of\n                    <i>Cryptococcus neoformans<\/i>"],"prefix":"10.1242","volume":"138","author":[{"given":"Deepika","family":"Kumari","sequence":"first","affiliation":[{"name":"Maharshi Dayanand University 1 Department of Biochemistry , , Rohtak, Haryana 124001 ,","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohit","family":"Kumar","sequence":"additional","affiliation":[{"name":"International Centre for Genetic Engineering and Biotechnology 2 , New Delhi 110067 ,","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naseem A.","family":"Gaur","sequence":"additional","affiliation":[{"name":"International Centre for Genetic Engineering and Biotechnology 2 , New Delhi 110067 ,","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lucky","family":"Duhan","sequence":"additional","affiliation":[{"name":"Maharshi Dayanand University 1 Department of Biochemistry , , Rohtak, Haryana 124001 ,","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nadezhda","family":"Sachivkina","sequence":"additional","affiliation":[{"name":"Peoples\u2019 Friendship University of Russia 3 Department of Microbiology , , Moscow , 117198","place":["Russia"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raman","family":"Manoharlal","sequence":"additional","affiliation":[{"name":"ITC Limited, ITC Life Science and Technology Centre (LSTC) 4 , Peenya Industrial Area, 1st Phase, Bengaluru, Karnataka, 560058 ,","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2851-5391","authenticated-orcid":false,"given":"Ritu","family":"Pasrija","sequence":"additional","affiliation":[{"name":"Maharshi Dayanand University 1 Department of Biochemistry , , Rohtak, Haryana 124001 ,","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","published-online":{"date-parts":[[2025,4,3]]},"reference":[{"key":"2026012910014414600_JCS263558C1","doi-asserted-by":"publisher","first-page":"2659","DOI":"10.15252\/embj.201489039","article-title":"The small GTPase Arf1 modulates mitochondrial morphology and function","volume":"33","author":"Ackema","year":"2014","journal-title":"EMBO J."},{"key":"2026012910014414600_JCS263558C2","doi-asserted-by":"publisher","first-page":"e0154280","DOI":"10.1371\/journal.pone.0154280","article-title":"Sar1, a novel regulator of ER-mitochondrial contact sites","volume":"11","author":"Ackema","year":"2016","journal-title":"PLoS ONE"},{"key":"2026012910014414600_JCS263558C3","doi-asserted-by":"publisher","first-page":"129","DOI":"10.1016\/j.bbrc.2017.05.021","article-title":"Crystal structure of Mdm12 and combinatorial reconstitution of Mdm12\/Mmm1 ERMES complexes for structural studies","volume":"488","author":"AhYoung","year":"2017","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"2026012910014414600_JCS263558C4","doi-asserted-by":"publisher","first-page":"1173","DOI":"10.1128\/EC.00103-15","article-title":"Virulence-associated enzymes of Cryptococcus neoformans","volume":"14","author":"Almeida","year":"2015","journal-title":"Eukaryot. Cell"},{"key":"2026012910014414600_JCS263558C5","doi-asserted-by":"publisher","first-page":"101519","DOI":"10.1016\/j.jbc.2021.101519","article-title":"Cryptococcus neoformans melanization incorporates multiple catecholamines to produce polytypic melanin","volume":"298","author":"Baker","year":"2022","journal-title":"J. Biol. Chem."},{"key":"2026012910014414600_JCS263558C6","doi-asserted-by":"publisher","first-page":"2412534121","DOI":"10.1073\/pnas.2412534121","article-title":"Cell wall melanin impedes growth of the Cryptococcus neoformans polysaccharide capsule by sequestering calcium","volume":"121","author":"Baker","year":"2024","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2026012910014414600_JCS263558C7","doi-asserted-by":"publisher","first-page":"22044","DOI":"10.1073\/pnas.1009845107","article-title":"Pathway analysis of Candida albicans survival and virulence determinants in a murine infection model","volume":"107","author":"Becker","year":"2010","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2026012910014414600_JCS263558C8","doi-asserted-by":"publisher","first-page":"545","DOI":"10.1083\/jcb.136.3.545","article-title":"Mdm12p, a component required for mitochondrial inheritance that is conserved between budding and fission yeast","volume":"136","author":"Berger","year":"1997","journal-title":"J. Cell Biol."},{"key":"2026012910014414600_JCS263558C9","doi-asserted-by":"publisher","first-page":"4618","DOI":"10.1091\/mbc.e03-04-0225","article-title":"A protein complex containing Mdm10p, Mdm12p, and Mmm1p links mitochondrial membranes and DNA to the cytoskeleton-based segregation machinery","volume":"14","author":"Boldogh","year":"2003","journal-title":"Mol. Biol. Cell"},{"key":"2026012910014414600_JCS263558C10","doi-asserted-by":"publisher","first-page":"697","DOI":"10.1111\/myc.12650","article-title":"An alternative method for the analysis of melanin production in Cryptococcus neoformans sensu lato and Cryptococcus gattii sensu lato","volume":"60","author":"Brilhante","year":"2017","journal-title":"Mycoses"},{"key":"2026012910014414600_JCS263558C11","doi-asserted-by":"publisher","first-page":"429","DOI":"10.1080\/13693780701885636","article-title":"Phospholipase activity of yeasts from wild birds and possible implications for human disease","volume":"46","author":"Cafarchia","year":"2008","journal-title":"Med. Mycol."},{"key":"2026012910014414600_JCS263558C12","doi-asserted-by":"publisher","first-page":"fov027","DOI":"10.1093\/femsyr\/fov027","article-title":"System-level impact of mitochondria on fungal virulence: to metabolism and beyond","volume":"15","author":"Calderone","year":"2015","journal-title":"FEMS Yeast Res."},{"key":"2026012910014414600_JCS263558C13","doi-asserted-by":"publisher","first-page":"e00080-15","DOI":"10.1128\/mSphere.00080-15","article-title":"The zinc finger protein Mig1 regulates mitochondrial function and azole drug susceptibility in the pathogenic fungus Cryptococcus neoformans","volume":"1","author":"Caza","year":"2016","journal-title":"mSphere"},{"key":"2026012910014414600_JCS263558C14","doi-asserted-by":"publisher","first-page":"744","DOI":"10.1128\/AAC.50.2.744-747.2006","article-title":"Inhibition of Candida parapsilosis mitochondrial respiratory pathways enhances susceptibility to caspofungin","volume":"50","author":"Chamilos","year":"2006","journal-title":"Antimicrob. Agents Chemother."},{"key":"2026012910014414600_JCS263558C15","doi-asserted-by":"publisher","first-page":"10.1128\/mbio.01375-18","DOI":"10.1128\/mBio.01375-18","article-title":"maintenance of mitochondrial morphology in Cryptococcus neoformans is critical for stress resistance and virulence","volume":"9","author":"Chang","year":"2018","journal-title":"mBio"},{"key":"2026012910014414600_JCS263558C16","doi-asserted-by":"publisher","first-page":"1112","DOI":"10.1111\/j.1365-2958.2009.07018.x","article-title":"Phosphatidylserine synthase and phosphatidylserine decarboxylase are essential for cell wall integrity and virulence in Candida albicans","volume":"75","author":"Chen","year":"2010","journal-title":"Mol. Microbiol."},{"key":"2026012910014414600_JCS263558C17","doi-asserted-by":"publisher","first-page":"4044","DOI":"10.1038\/s41598-017-04290-8","article-title":"A novel bZIP protein, Gsb1, is required for oxidative stress response, mating, and virulence in the human pathogen Cryptococcus neoformans","volume":"7","author":"Cheon","year":"2017","journal-title":"Sci. Rep."},{"key":"2026012910014414600_JCS263558C18","doi-asserted-by":"publisher","first-page":"968","DOI":"10.1111\/j.1365-2958.2010.07503.x","article-title":"Cell wall integrity is linked to mitochondria and phospholipid homeostasis in Candida albicans through the activity of the post-transcriptional regulator Ccr4-Pop2","volume":"79","author":"Dagley","year":"2011","journal-title":"Mol. Microbiol."},{"key":"2026012910014414600_JCS263558C19","doi-asserted-by":"publisher","first-page":"243","DOI":"10.1186\/s12866-019-1606-4","article-title":"Increase of reactive oxygen species contributes to growth inhibition by fluconazole in Cryptococcus neoformans","volume":"19","author":"Dbouk","year":"2019","journal-title":"BMC Microbiol."},{"key":"2026012910014414600_JCS263558C20","doi-asserted-by":"publisher","first-page":"657502","DOI":"10.3389\/fcimb.2021.657502","article-title":"Cryptococcal virulence in humans: learning from translational studies with clinical isolates","volume":"11","author":"de Sousa","year":"2021","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"2026012910014414600_JCS263558C21","doi-asserted-by":"publisher","first-page":"393","DOI":"10.3390\/jof8040393","article-title":"Faster Cryptococcus melanization increases virulence in experimental and human cryptococcosis","volume":"8","author":"de Sousa","year":"2022","journal-title":"J. Fungi (Basel)"},{"key":"2026012910014414600_JCS263558C22","doi-asserted-by":"publisher","first-page":"1067","DOI":"10.1128\/EC.3.5.1067-1075.2004","article-title":"Role of phagocytosis in the virulence of Cryptococcus neoformans","volume":"3","author":"Del Poeta","year":"2004","journal-title":"Eukaryot. Cell"},{"key":"2026012910014414600_JCS263558C23","doi-asserted-by":"publisher","first-page":"1142","DOI":"10.1016\/S0140-6736(03)14472-8","article-title":"Echinocandin antifungal drugs","volume":"362","author":"Denning","year":"2003","journal-title":"Lancet"},{"key":"2026012910014414600_JCS263558C24","doi-asserted-by":"publisher","first-page":"e428","DOI":"10.1016\/S1473-3099(23)00692-8","article-title":"Global incidence and mortality of severe fungal disease","volume":"24","author":"Denning","year":"2024","journal-title":"Lancet Infect. Dis."},{"key":"2026012910014414600_JCS263558C25","doi-asserted-by":"publisher","first-page":"11","DOI":"10.1016\/j.fgb.2014.12.006","article-title":"Leu1 plays a role in iron metabolism and is required for virulence in Cryptococcus neoformans","volume":"75","author":"Do","year":"2015","journal-title":"Fungal Genet. Biol."},{"key":"2026012910014414600_JCS263558C26","doi-asserted-by":"publisher","first-page":"706","DOI":"10.1016\/j.bbrc.2016.06.123","article-title":"The lysine biosynthetic enzyme Lys4 influences iron metabolism, mitochondrial function and virulence in Cryptococcus neoformans","volume":"477","author":"Do","year":"2016","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"2026012910014414600_JCS263558C27","doi-asserted-by":"publisher","first-page":"95","DOI":"10.1016\/j.devcel.2014.06.007","article-title":"A dynamic interface between vacuoles and mitochondria in yeast","volume":"30","author":"Elbaz-Alon","year":"2014","journal-title":"Dev. Cell"},{"key":"2026012910014414600_JCS263558C28","doi-asserted-by":"publisher","first-page":"418","DOI":"10.3390\/jof7060418","article-title":"The vacuolar morphogenesis protein Vam6-Like protein Vlp1 is required for pathogenicity of Cryptococcus neoformans","volume":"7","author":"Fan","year":"2021","journal-title":"J. Fungi (Basel)"},{"key":"2026012910014414600_JCS263558C29","doi-asserted-by":"publisher","first-page":"1036","DOI":"10.1128\/EC.2.5.1036-1045.2003","article-title":"Recapitulation of the sexual cycle of the primary fungal pathogen Cryptococcus neoformans var. gattii: implications for an outbreak on vancouver island, Canada","volume":"2","author":"Fraser","year":"2003","journal-title":"Eukaryot. Cell"},{"key":"2026012910014414600_JCS263558C30","doi-asserted-by":"publisher","first-page":"358","DOI":"10.1126\/science.1207385","article-title":"ER tubules mark sites of mitochondrial division","volume":"334","author":"Friedman","year":"2011","journal-title":"Science"},{"key":"2026012910014414600_JCS263558C31","doi-asserted-by":"publisher","first-page":"37","DOI":"10.1016\/j.ijmm.2016.11.005","article-title":"The ER-mitochondria encounter structure contributes to hyphal growth, mitochondrial morphology and virulence of the pathogenic mold Aspergillus fumigatus","volume":"307","author":"Gei\u00dfel","year":"2017","journal-title":"Int. J. Med. Microbiol."},{"key":"2026012910014414600_JCS263558C32","doi-asserted-by":"publisher","first-page":"57171","DOI":"10.3791\/57171","article-title":"Size matters: measurement of capsule diameter in Cryptococcus neoformans","volume":"132","author":"Guess","year":"2018","journal-title":"J. Vis. Exp."},{"key":"2026012910014414600_JCS263558C33","doi-asserted-by":"publisher","first-page":"4928","DOI":"10.1038\/s41598-023-32140-3","article-title":"Differentiated extracts from freshwater and terrestrial mollusks inhibit virulence factor production in Cryptococcus neoformans","volume":"13","author":"Gutierrez-Gongora","year":"2023","journal-title":"Sci. Rep."},{"key":"2026012910014414600_JCS263558C34","doi-asserted-by":"publisher","first-page":"634","DOI":"10.18632\/oncotarget.27366","article-title":"Human insulin modulates \u03b1-synuclein aggregation via DAF-2\/DAF-16 signalling pathway by antagonising DAF-2 receptor in C. elegans model of Parkinson's disease","volume":"11","author":"Haque","year":"2020","journal-title":"Oncotarget"},{"key":"2026012910014414600_JCS263558C35","doi-asserted-by":"publisher","first-page":"e1002411","DOI":"10.1371\/journal.ppat.1002411","article-title":"Toward an integrated model of capsule regulation in Cryptococcus neoformans","volume":"7","author":"Haynes","year":"2011","journal-title":"PLoS Pathog."},{"key":"2026012910014414600_JCS263558C36","doi-asserted-by":"publisher","first-page":"907","DOI":"10.1128\/iai.59.3.907-912.1991","article-title":"Differential adherence of hydrophobic and hydrophilic Candida albicans yeast cells to mouse tissues","volume":"59","author":"Hazen","year":"1991","journal-title":"Infect. Immun."},{"key":"2026012910014414600_JCS263558C37","doi-asserted-by":"publisher","first-page":"401","DOI":"10.1083\/jcb.152.2.401","article-title":"Mmm1p, a mitochondrial outer membrane protein, is connected to mitochondrial DNA (mtDNA) nucleoids and required for mtDNA stability","volume":"152","author":"Hobbs","year":"2001","journal-title":"J. Cell. Biol."},{"key":"2026012910014414600_JCS263558C38","doi-asserted-by":"publisher","first-page":"10.1128\/mbio.01127-20","DOI":"10.1128\/mBio.01127-20","article-title":"The novel J-domain protein Mrj1 is required for mitochondrial respiration and virulence in Cryptococcus neoformans","volume":"11","author":"Horianopoulos","year":"2020","journal-title":"mBio"},{"key":"2026012910014414600_JCS263558C39","doi-asserted-by":"publisher","first-page":"772","DOI":"10.3390\/jof9070772","article-title":"Antifungal effect of vitamin D3 against Cryptococcus neoformans coincides with reduced biofilm formation, compromised cell wall integrity, and increased generation of reactive oxygen species","volume":"9","author":"Huang","year":"2023","journal-title":"J. Fungi (Basel)"},{"key":"2026012910014414600_JCS263558C40","doi-asserted-by":"publisher","first-page":"10.1128\/mbio.02334-14","DOI":"10.1128\/mBio.02334-14","article-title":"Essential gene discovery in the basidiomycete Cryptococcus neoformans for antifungal drug target prioritization","volume":"6","author":"Ianiri","year":"2015","journal-title":"mBio"},{"key":"2026012910014414600_JCS263558C41","doi-asserted-by":"publisher","first-page":"3081","DOI":"10.1038\/srep03081","article-title":"A simple assay for measuring catalase activity: a visual approach","volume":"3","author":"Iwase","year":"2013","journal-title":"Sci. Rep."},{"key":"2026012910014414600_JCS263558C42","doi-asserted-by":"publisher","first-page":"951","DOI":"10.5455\/ijmsph.2017.1267623012017","article-title":"Detection of phospholipase production by egg Yolk-Agar in Malassezia isolates from diseased and healthy human host","volume":"6","author":"Jain","year":"2017","journal-title":"Int. J. Med. Sci. Public Health"},{"key":"2026012910014414600_JCS263558C43","doi-asserted-by":"publisher","first-page":"E9502","DOI":"10.1073\/pnas.1715592114","article-title":"Crystal structures of Mmm1 and Mdm12\u2013Mmm1 reveal mechanistic insight into phospholipid trafficking at ER-mitochondria contact sites","volume":"114","author":"Jeong","year":"2017","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2026012910014414600_JCS263558C44","doi-asserted-by":"publisher","first-page":"2499","DOI":"10.1007\/s00204-023-03562-9","article-title":"Reactive oxygen species, toxicity, oxidative stress, and antioxidants: chronic diseases and aging","volume":"97","author":"Jomova","year":"2023","journal-title":"Arch. Toxicol."},{"key":"2026012910014414600_JCS263558C45","doi-asserted-by":"publisher","first-page":"8132","DOI":"10.1038\/s41598-018-26405-5","article-title":"Evolutionarily conserved and divergent roles of unfolded protein response (UPR) in the pathogenic Cryptococcus species complex","volume":"8","author":"Jung","year":"2018","journal-title":"Sci. Rep."},{"key":"2026012910014414600_JCS263558C46","doi-asserted-by":"publisher","first-page":"162","DOI":"10.1186\/s12866-022-02579-x","article-title":"Caenorhabditis elegans DAF-16 regulates lifespan and immune responses to Cryptococcus neoformans and Cryptococcus gattii infections","volume":"22","author":"Kitisin","year":"2022","journal-title":"BMC Microbiol."},{"key":"2026012910014414600_JCS263558C47","doi-asserted-by":"publisher","first-page":"2555","DOI":"10.3389\/fmicb.2017.02555","article-title":"The mitochondrial GTPase Gem1 contributes to the cell wall stress response and invasive growth of Candida albicans","volume":"8","author":"Koch","year":"2017","journal-title":"Front. Microbiol."},{"key":"2026012910014414600_JCS263558C48","doi-asserted-by":"publisher","first-page":"2071","DOI":"10.1038\/s44319-024-00113-w","article-title":"The ER-SURF pathway uses ER-mitochondria contact sites for protein targeting to mitochondria","volume":"25","author":"Koch","year":"2024","journal-title":"EMBO Rep."},{"key":"2026012910014414600_JCS263558C49","doi-asserted-by":"publisher","first-page":"477","DOI":"10.1126\/science.1175088","article-title":"An ER-mitochondria tethering complex revealed by a synthetic biology screen","volume":"325","author":"Kornmann","year":"2009","journal-title":"Science"},{"key":"2026012910014414600_JCS263558C50","doi-asserted-by":"publisher","first-page":"14151","DOI":"10.1073\/pnas.1111314108","article-title":"The conserved GTPase Gem1 regulates endoplasmic reticulum\u2013mitochondria connections","volume":"108","author":"Kornmann","year":"2011","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2026012910014414600_JCS263558C51","doi-asserted-by":"publisher","first-page":"165","DOI":"10.3390\/pathogens10020165","article-title":"The minimum inhibitory concentration of antibiotics: methods, interpretation, clinical relevance","volume":"10","author":"Kowalska-Krochmal","year":"2021","journal-title":"Pathogens"},{"key":"2026012910014414600_JCS263558C52","doi-asserted-by":"publisher","first-page":"355","DOI":"10.1007\/s00203-024-04080-7","article-title":"Investigation of the influence of pH and temperature on melanization and survival under oxidative stress in Cryptococcus neoformans","volume":"206","author":"Kumari","year":"2024","journal-title":"Arch. Microbiol."},{"key":"2026012910014414600_JCS263558C53","doi-asserted-by":"publisher","first-page":"89","DOI":"10.1016\/j.mito.2020.02.010","article-title":"The ERMES (endoplasmic reticulum and mitochondria encounter structures) mediated functions in fungi","volume":"52","author":"Kundu","year":"2020","journal-title":"Mitochondrion"},{"key":"2026012910014414600_JCS263558C54","doi-asserted-by":"publisher","first-page":"121","DOI":"10.1007\/s12223-019-00713-3","article-title":"Phospholipid biosynthesis disruption renders the yeast cells sensitive to antifungals","volume":"65","author":"Kundu","year":"2020","journal-title":"Folia Microbiol."},{"key":"2026012910014414600_JCS263558C55","doi-asserted-by":"publisher","first-page":"883","DOI":"10.1083\/jcb.201502105","article-title":"ER-mitochondrial junctions can be bypassed by dominant mutations in the endosomal protein Vps13","volume":"210","author":"Lang","year":"2015","journal-title":"J. Cell Biol."},{"key":"2026012910014414600_JCS263558C56","doi-asserted-by":"publisher","first-page":"225","DOI":"10.1093\/mmy\/myu083","article-title":"Generation of stable mutants and targeted gene deletion strains in Cryptococcus neoformans through electroporation","volume":"53","author":"Lin","year":"2015","journal-title":"Med. Mycol."},{"key":"2026012910014414600_JCS263558C57","doi-asserted-by":"publisher","first-page":"e04562-22","DOI":"10.1128\/spectrum.04562-22","article-title":"Antifungal peptide SP1 damages polysaccharide capsule of Cryptococcus neoformans and enhances phagocytosis of macrophages","volume":"11","author":"Liu","year":"2023","journal-title":"Microbiol. Spectr."},{"key":"2026012910014414600_JCS263558C58","doi-asserted-by":"publisher","first-page":"e3000957","DOI":"10.1371\/journal.pbio.3000957","article-title":"An evolutionarily diverged mitochondrial protein controls biofilm growth and virulence in Candida albicans","volume":"19","author":"Mamouei","year":"2021","journal-title":"PLoS Biol."},{"key":"2026012910014414600_JCS263558C59","doi-asserted-by":"publisher","first-page":"273","DOI":"10.1016\/j.jmb.2018.11.002","article-title":"Contacts in death: the role of the ER\u2013mitochondria axis in acetic acid-induced apoptosis in yeast","volume":"431","author":"Martins","year":"2019","journal-title":"J. Mol. Biol."},{"key":"2026012910014414600_JCS263558C60","doi-asserted-by":"publisher","first-page":"2072","DOI":"10.1016\/j.jmb.2015.03.004","article-title":"Genome-wide localization study of yeast pex11 identifies peroxisome-mitochondria interactions through the ERMES complex","volume":"427","author":"Mattiazzi U\u0161aj","year":"2015","journal-title":"J. Mol. Biol."},{"key":"2026012910014414600_JCS263558C61","doi-asserted-by":"publisher","first-page":"1366","DOI":"10.1080\/21505594.2020.1831332","article-title":"A mutation in C. neoformans mitochondrial NADH dehydrogenase results in increased virulence in mice","volume":"11","author":"Merryman","year":"2020","journal-title":"Virulence"},{"key":"2026012910014414600_JCS263558C62","doi-asserted-by":"publisher","first-page":"e112408","DOI":"10.5812\/jjm.112408","article-title":"Predominance of Cryptococcus neoformans Var. grubii in Ahvaz, molecular identification and evaluation of virulence factors","volume":"13","author":"Moslem","year":"2020","journal-title":"Jundishapur J. Microbiol."},{"key":"2026012910014414600_JCS263558C63","doi-asserted-by":"publisher","first-page":"670","DOI":"10.3390\/biom14060670","article-title":"Mitochondrial reactive oxygen species in infection and immunity","volume":"14","author":"Mukherjee","year":"2024","journal-title":"Biomolecules"},{"key":"2026012910014414600_JCS263558C64","doi-asserted-by":"publisher","first-page":"e00422","DOI":"10.7554\/eLife.00422","article-title":"ER-associated mitochondrial division links the distribution of mitochondria and mitochondrial DNA in yeast","volume":"2","author":"Murley","year":"2013","journal-title":"eLife"},{"key":"2026012910014414600_JCS263558C65","doi-asserted-by":"publisher","first-page":"15675","DOI":"10.1073\/pnas.232568599","article-title":"Killing of Caenorhabditis elegans by Cryptococcus neoformans as a model of yeast pathogenesis","volume":"99","author":"Mylonakis","year":"2002","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2026012910014414600_JCS263558C66","doi-asserted-by":"publisher","first-page":"880","DOI":"10.1111\/j.1600-0854.2012.01352.x","article-title":"Gem1 and ERMES do not directly affect phosphatidylserine transport from ER to mitochondria or mitochondrial inheritance","volume":"13","author":"Nguyen","year":"2012","journal-title":"Traffic"},{"key":"2026012910014414600_JCS263558C67","doi-asserted-by":"publisher","first-page":"240","DOI":"10.3390\/jof9020240","article-title":"In Candida glabrata, ERMES component GEM1 controls mitochondrial morphology, mtROS, and drug efflux pump expression, resulting in azole susceptibility","volume":"9","author":"Okamoto","year":"2023","journal-title":"J. Fungi"},{"key":"2026012910014414600_JCS263558C68","doi-asserted-by":"publisher","first-page":"7","DOI":"10.1080\/00362178285380031","article-title":"Plate method for detection of phospholipase activity in Candida albicans","volume":"20","author":"Price","year":"1982","journal-title":"Sabouraudia"},{"key":"2026012910014414600_JCS263558C69","doi-asserted-by":"publisher","first-page":"2961","DOI":"10.1091\/mbc.11.9.2961","article-title":"Role of MMM1 in maintaining mitochondrial morphology in Neurospora crassa","volume":"11","author":"Prokisch","year":"2000","journal-title":"Mol. Biol. Cell."},{"key":"2026012910014414600_JCS263558C70","doi-asserted-by":"publisher","first-page":"e21945","DOI":"10.1371\/journal.pone.0021945","article-title":"Hydrogen peroxide acts on sensitive mitochondrial proteins to induce death of a fungal pathogen revealed by proteomic analysis","volume":"6","author":"Qin","year":"2011","journal-title":"PLoS ONE"},{"key":"2026012910014414600_JCS263558C71","doi-asserted-by":"publisher","first-page":"521","DOI":"10.1038\/s41467-020-20866-x","article-title":"Emr1 regulates the number of foci of the endoplasmic reticulum-mitochondria encounter structure complex","volume":"12","author":"Rasul","year":"2021","journal-title":"Nat. Commun."},{"key":"2026012910014414600_JCS263558C72","doi-asserted-by":"publisher","first-page":"1401","DOI":"10.1046\/j.1365-2958.2003.03780.x","article-title":"Cell wall \u03b1-1,3-glucan is required to anchor the Cryptococcus neoformans capsule","volume":"50","author":"Reese","year":"2003","journal-title":"Mol. Microbiol."},{"key":"2026012910014414600_JCS263558C73","doi-asserted-by":"publisher","first-page":"e00744-21","DOI":"10.1128\/Spectrum.00744-21","article-title":"Caspofungin inhibits mixed biofilms of Candida albicans and methicillin-resistant Staphylococcus aureus and displays effectiveness in coinfected Galleria mellonella larvae","volume":"9","author":"Scheunemann","year":"2021","journal-title":"Microbiol. Spectr."},{"key":"2026012910014414600_JCS263558C74","doi-asserted-by":"publisher","first-page":"1376","DOI":"10.1128\/EC.05184-11","article-title":"Mitochondria and fungal pathogenesis: drug tolerance, virulence, and potential for antifungal therapy","volume":"10","author":"Shingu-Vazquez","year":"2011","journal-title":"Eukaryot. Cell."},{"key":"2026012910014414600_JCS263558C75","doi-asserted-by":"publisher","first-page":"965","DOI":"10.15252\/embr.201541273","article-title":"Mcp3 is a novel mitochondrial outer membrane protein that follows a unique IMP-dependent biogenesis pathway","volume":"17","author":"Sinzel","year":"2016","journal-title":"EMBO Rep."},{"key":"2026012910014414600_JCS263558C76","doi-asserted-by":"publisher","first-page":"127892","DOI":"10.1016\/j.micres.2024.127892","article-title":"Endoplasmic reticulum-mitochondrial encounter structure regulates the mitochondrial morphology, DON biosynthesis and toxisome formation in Fusarium graminearum","volume":"289","author":"Song","year":"2024","journal-title":"Microbiol. Res."},{"key":"2026012910014414600_JCS263558C77","doi-asserted-by":"publisher","first-page":"3563","DOI":"10.1242\/jcs.121244","article-title":"Mcp1 and Mcp2, two novel proteins involved in mitochondrial lipid homeostasis","volume":"126","author":"Tan","year":"2013","journal-title":"J. Cell Sci."},{"key":"2026012910014414600_JCS263558C78","doi-asserted-by":"publisher","first-page":"1384","DOI":"10.1128\/AAC.50.4.1384-1392.2006","article-title":"Candida glabrata PDR1, a transcriptional regulator of a pleiotropic drug resistance network, mediates azole resistance in clinical isolates and petite mutants","volume":"50","author":"Tsai","year":"2006","journal-title":"Antimicrob. Agents Chemother."},{"key":"2026012910014414600_JCS263558C79","doi-asserted-by":"publisher","first-page":"e00016","DOI":"10.1128\/mSphere.00074-16","article-title":"The endoplasmic reticulum-mitochondrion tether ERMES orchestrates fungal immune evasion, illuminating inflammasome responses to hyphal signals","volume":"1","author":"Tucey","year":"2016","journal-title":"mSphere"},{"key":"2026012910014414600_JCS263558C80","doi-asserted-by":"publisher","first-page":"426","DOI":"10.1080\/21505594.2017.1414133","article-title":"Exploring and exploiting the connection between mitochondria and the virulence of human pathogenic fungi","volume":"9","author":"Verma","year":"2018","journal-title":"Virulence"},{"key":"2026012910014414600_JCS263558C81","doi-asserted-by":"publisher","first-page":"e00310-20","DOI":"10.1128\/mSphere.00310-20","article-title":"Variation in cell surface hydrophobicity among Cryptococcus neoformans strains influences interactions with Amoebas","volume":"5","author":"Vij","year":"2020","journal-title":"mSphere"},{"key":"2026012910014414600_JCS263558C82","doi-asserted-by":"publisher","first-page":"171","DOI":"10.1186\/s12866-015-0504-7","article-title":"Changes in the hemolytic activity of Candida species by common electrolytes","volume":"15","author":"Wan","year":"2015","journal-title":"BMC Microbiol."},{"key":"2026012910014414600_JCS263558C83","doi-asserted-by":"publisher","first-page":"2044","DOI":"10.1093\/molbev\/mst120","article-title":"The ancient and widespread nature of the ER-mitochondria encounter structure","volume":"30","author":"Wideman","year":"2013","journal-title":"Mol. Biol. Evol."},{"key":"2026012910014414600_JCS263558C84","doi-asserted-by":"publisher","first-page":"188","DOI":"10.1038\/s41586-023-06050-3","article-title":"In situ architecture of the ER-mitochondria encounter structure","volume":"618","author":"Wozny","year":"2023","journal-title":"Nature"},{"key":"2026012910014414600_JCS263558C85","doi-asserted-by":"publisher","first-page":"848","DOI":"10.1016\/j.funbio.2012.05.002","article-title":"CDR4 is the major contributor to azole resistance among four Pdr5p-like ABC transporters in Neurospora crassa","volume":"116","author":"Zhang","year":"2012","journal-title":"Fungal Biol."},{"key":"2026012910014414600_JCS263558C86","doi-asserted-by":"publisher","first-page":"2004","DOI":"10.1111\/febs.14445","article-title":"Arf1 regulates the ER-mitochondria encounter structure (ERMES) in a reactive oxygen species-dependent manner","volume":"285","author":"Zhang","year":"2018","journal-title":"FEBS J."},{"key":"2026012910014414600_JCS263558C87","doi-asserted-by":"publisher","first-page":"20","DOI":"10.1186\/s40249-023-01073-4","article-title":"Cryptococcus neoformans, a global threat to human health","volume":"12","author":"Zhao","year":"2023","journal-title":"Infect. Dis. Poverty."},{"key":"2026012910014414600_JCS263558C88","doi-asserted-by":"publisher","first-page":"957857","DOI":"10.3389\/fmicb.2022.957857","article-title":"Requirement of a putative mitochondrial GTPase, GemA, for azole susceptibility, virulence, and cell wall integrity in Aspergillus fumigatus","volume":"13","author":"Zhou","year":"2022","journal-title":"Front. Microbiol."}],"container-title":["Journal of Cell Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.biologists.com\/jcs\/article-pdf\/doi\/10.1242\/jcs.263558\/3642872\/jcs263558.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/journals.biologists.com\/jcs\/article-pdf\/doi\/10.1242\/jcs.263558\/3642872\/jcs263558.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,29]],"date-time":"2026-01-29T15:01:53Z","timestamp":1769698913000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.biologists.com\/jcs\/article\/138\/9\/jcs263558\/367586\/ER-mitochondria-encounter-structure-connections"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,4,3]]},"references-count":88,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2025,5,1]]}},"URL":"https:\/\/doi.org\/10.1242\/jcs.263558","relation":{},"ISSN":["0021-9533","1477-9137"],"issn-type":[{"value":"0021-9533","type":"print"},{"value":"1477-9137","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,4,3]]},"article-number":"jcs263558"}}