{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T21:35:51Z","timestamp":1781213751401,"version":"3.54.1"},"reference-count":109,"publisher":"The Company of Biologists","issue":"6","license":[{"start":{"date-parts":[[2025,3,15]],"date-time":"2025-03-15T00:00:00Z","timestamp":1741996800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"funder":[{"DOI":"10.13039\/501100001459","name":"Ministry of Education, Singapore","doi-asserted-by":"crossref","award":["T2 MOE2019-T2-2-045"],"award-info":[{"award-number":["T2 MOE2019-T2-2-045"]}],"id":[{"id":"10.13039\/501100001459","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001459","name":"Ministry of Education, Singapore","doi-asserted-by":"crossref","award":["T1 R-154-000-B42-114"],"award-info":[{"award-number":["T1 R-154-000-B42-114"]}],"id":[{"id":"10.13039\/501100001459","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,3,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>When starved of nitrogen, cells of the fission yeast Schizosaccharomyces pombe enter a quiescent \u2018G0\u2019 state with smaller nuclei and transcriptional repression. The genomics of S. pombe G0 cells has been well studied, but much of its nuclear cell biology remains unknown. Here, we use confocal microscopy, immunoblots and electron cryotomography to investigate the cytological, biochemical and ultrastructural differences between S. pombe proliferating, G1-arrested and G0 cell nuclei, with an emphasis on the histone acetylation, RNA polymerase II fates and macromolecular complex packing. Compared to proliferating cells, G0 cells have lower levels of histone acetylation, nuclear RNA polymerase II and active transcription. The G0 nucleus has similar macromolecular crowding yet fewer chromatin-associated multi-megadalton globular complexes. Induced histone hyperacetylation during nitrogen starvation results in cells that have larger nuclei and therefore chromatin that is less compact. However, these histone-hyperacetylated cells remain transcriptionally repressed with similar nuclear crowding. Canonical nucleosomes \u2013 those that resemble the crystal structure \u2013 are rare in proliferating, G1-arrested and G0 cells. Our study therefore shows that extreme changes in nucleus physiology are possible without extreme reorganization at the macromolecular level.<\/jats:p>","DOI":"10.1242\/jcs.263654","type":"journal-article","created":{"date-parts":[[2025,2,27]],"date-time":"2025-02-27T04:33:46Z","timestamp":1740630826000},"source":"Crossref","is-referenced-by-count":5,"title":["Macromolecular and cytological changes in fission yeast G0 nuclei"],"prefix":"10.1242","volume":"138","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5297-4427","authenticated-orcid":false,"given":"Zhi Yang","family":"Tan","sequence":"first","affiliation":[{"name":"National University of Singapore Department of Biological Sciences and Centre for BioImaging Sciences , , 117543","place":["Singapore"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8558-6279","authenticated-orcid":false,"given":"Shujun","family":"Cai (\u8521\u8212\u541b)","sequence":"additional","affiliation":[{"name":"National University of Singapore Department of Biological Sciences and Centre for BioImaging Sciences , , 117543","place":["Singapore"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-9621-0689","authenticated-orcid":false,"given":"Saayli A.","family":"Paithankar","sequence":"additional","affiliation":[{"name":"National University of Singapore Department of Biological Sciences and Centre for BioImaging Sciences , , 117543","place":["Singapore"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8371-9827","authenticated-orcid":false,"given":"Tingsheng","family":"Liu","sequence":"additional","affiliation":[{"name":"National University of Singapore Department of Biological Sciences and Centre for BioImaging Sciences , , 117543","place":["Singapore"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Nie","sequence":"additional","affiliation":[{"name":"National University of Singapore Department of Biological Sciences and Centre for BioImaging Sciences , , 117543","place":["Singapore"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3810-5302","authenticated-orcid":false,"given":"Jian","family":"Shi","sequence":"additional","affiliation":[{"name":"National University of Singapore Department of Biological Sciences and Centre for BioImaging Sciences , , 117543","place":["Singapore"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8685-4896","authenticated-orcid":false,"given":"Lu","family":"Gan (\u7518\u9732)","sequence":"additional","affiliation":[{"name":"National University of Singapore Department of Biological Sciences and Centre for BioImaging Sciences , , 117543","place":["Singapore"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2025,3,27]]},"reference":[{"key":"2025032704370832300_JCS263654C1","doi-asserted-by":"publisher","first-page":"89","DOI":"10.1083\/jcb.200604072","article-title":"Isolation of quiescent and nonquiescent cells from yeast stationary-phase cultures","volume":"174","author":"Allen","year":"2006","journal-title":"J. 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