{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,14]],"date-time":"2026-04-14T18:23:03Z","timestamp":1776190983484,"version":"3.50.1"},"reference-count":80,"publisher":"The Company of Biologists","issue":"20","license":[{"start":{"date-parts":[[2025,10,6]],"date-time":"2025-10-06T00:00:00Z","timestamp":1759708800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"name":"National Cancer Institute","award":["P30CA013330"],"award-info":[{"award-number":["P30CA013330"]}]},{"DOI":"10.13039\/100003287","name":"Rally Foundation","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100003287","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100007319","name":"Albert Einstein College of Medicine","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100007319","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,10,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>The thymus originates from the third pharyngeal pouch endoderm, which also gives rise to respiratory tract elements. Here, we examined intrathymic cystic structures, long considered remnants of organogenesis. Through sequential histology and ultrastructural imaging, we uncovered that these \u2018cysts\u2019 are in fact continuous and structured epithelial networks embedded within the thymic parenchyma. These networks follow a conserved \u2018head-neck-funnel-tentacle\u2019 architecture spanning the trabeculae, cortex, corticomedullary junction (CMJ) and medulla. The head, typically glandular and ciliated, connects to a funnel enriched in diverse epithelial cell types \u2013 goblet, tuft, club, ionocyte-like, microfold and ciliated cells \u2013 at the CMJ. Tentacle-like projections sometimes extend into the medulla, often surrounding perivascular spaces. Luminal contents vary, with thymocytes and macrophages most abundant caudally. We also identified solitary medullary thymic epithelial cells with large ciliated cytoplasmic lumens, distinct from these epithelial networks. Electron microscopy suggested a respiratory identity and thymic-specific adaptations for the lining cells. These findings challenge the notion of thymic cysts as inert debris, and instead reveal a coherent, mimetic system with possible roles in thymocyte selection, maturation and egress.<\/jats:p>","DOI":"10.1242\/jcs.264079","type":"journal-article","created":{"date-parts":[[2025,8,29]],"date-time":"2025-08-29T13:40:14Z","timestamp":1756474814000},"source":"Crossref","is-referenced-by-count":4,"title":["Beyond cysts \u2013 organization of epithelial networks in the murine thymus"],"prefix":"10.1242","volume":"138","author":[{"given":"Stepan","family":"Vodopyanov","sequence":"first","affiliation":[{"name":"Albert Einstein College of Medicine 1 Department of Microbiology and Immunology , , Bronx, NY 10461 ,","place":["USA"]},{"name":"Integrated Imaging Program for Cancer Research, Montefiore-Einstein Comprehensive Cancer Center 2 , Bronx, NY 10461 ,","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leslie","family":"Gunther-Cummins","sequence":"additional","affiliation":[{"name":"Analytical Imaging Facility, Albert Einstein College of Medicine 3 , Bronx, NY 10461 ,","place":["USA"]},{"name":"Albert Einstein College of Medicine 4 Department of Cell Biology , , Bronx, NY 10461 ,","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sophia","family":"DesMarais","sequence":"additional","affiliation":[{"name":"Albert Einstein College of Medicine 1 Department of Microbiology and Immunology , , Bronx, NY 10461 ,","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria K.","family":"Lagou","sequence":"additional","affiliation":[{"name":"Analytical Imaging Facility, Albert Einstein College of Medicine 3 , Bronx, NY 10461 ,","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xheni","family":"Nishku","sequence":"additional","affiliation":[{"name":"Analytical Imaging Facility, Albert Einstein College of Medicine 3 , Bronx, NY 10461 ,","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joseph","family":"Churaman","sequence":"additional","affiliation":[{"name":"Analytical Imaging Facility, Albert Einstein College of Medicine 3 , Bronx, NY 10461 ,","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hillary","family":"Guzik","sequence":"additional","affiliation":[{"name":"Analytical Imaging Facility, Albert Einstein College of Medicine 3 , Bronx, NY 10461 ,","place":["USA"]},{"name":"Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine 5 , Bronx, NY 10461 ,","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rotem","family":"Alon","sequence":"additional","affiliation":[{"name":"Analytical Imaging Facility, Albert Einstein College of Medicine 3 , Bronx, NY 10461 ,","place":["USA"]},{"name":"Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine 5 , Bronx, NY 10461 ,","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vera","family":"DesMarais","sequence":"additional","affiliation":[{"name":"Analytical Imaging Facility, Albert Einstein College of Medicine 3 , Bronx, NY 10461 ,","place":["USA"]},{"name":"Albert Einstein College of Medicine 4 Department of Cell Biology , , Bronx, NY 10461 ,","place":["USA"]},{"name":"Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine 5 , Bronx, NY 10461 ,","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frank","family":"Macaluso","sequence":"additional","affiliation":[{"name":"Analytical Imaging Facility, Albert Einstein College of Medicine 3 , Bronx, NY 10461 ,","place":["USA"]},{"name":"Albert Einstein College of Medicine 4 Department of Cell Biology , , Bronx, NY 10461 ,","place":["USA"]},{"name":"Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine 5 , Bronx, NY 10461 ,","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8808-707X","authenticated-orcid":false,"given":"George S.","family":"Karagiannis","sequence":"additional","affiliation":[{"name":"Albert Einstein College of Medicine 1 Department of Microbiology and Immunology , , Bronx, NY 10461 ,","place":["USA"]},{"name":"Integrated Imaging Program for Cancer Research, Montefiore-Einstein Comprehensive Cancer Center 2 , Bronx, NY 10461 ,","place":["USA"]},{"name":"Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine 5 , Bronx, NY 10461 ,","place":["USA"]},{"name":"Cancer Dormancy Institute, Montefiore-Einstein Comprehensive Cancer, Center 6 , Bronx, NY 10461 ,","place":["USA"]},{"name":"Marilyn and Stanley M. Katz Institute for Immunotherapy for Cancer and Inflammatory Disorders, Montefiore-Einstein Comprehensive Cancer Center 7 , Bronx, NY 10461 ,","place":["USA"]},{"name":"Montefiore-Einstein Comprehensive Cancer Center 8 Tumor Microenvironment and Metastasis Program , , Bronx, NY 10461 ,","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","published-online":{"date-parts":[[2025,10,6]]},"reference":[{"key":"2025123010413478200_JCS264079C1","doi-asserted-by":"publisher","first-page":"584","DOI":"10.1038\/s41467-025-55936-5","article-title":"Tracheal tuft cells release ATP and link innate to adaptive immunity in pneumonia","volume":"16","author":"Abdel Wadood","year":"2025","journal-title":"Nat. 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