{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T20:20:46Z","timestamp":1791490846631,"version":"4.3.4"},"reference-count":70,"publisher":"The Company of Biologists","issue":"12","license":[{"start":{"date-parts":[[2026,3,4]],"date-time":"2026-03-04T00:00:00Z","timestamp":1772582400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["R35GM154928"],"award-info":[{"award-number":["R35GM154928"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Center for 3D Structure and Physics of the Genome","award":["1UM1 HG011536"],"award-info":[{"award-number":["1UM1 HG011536"]}]},{"DOI":"10.13039\/100008975","name":"University of Massachusetts Amherst","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100008975","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,6,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>The structural integrity of the nucleus is dependent on nuclear mechanical elements of chromatin and lamins resisting antagonistic actin cytoskeleton forces. Force imbalance results in nuclear blebbing, rupture and cellular dysfunction found in many human diseases. Here, we used the fluorescent ubiquitin cell cycle indicator (FUCCI) cells to determine how cell cycle changes affect the nucleus and actin force balance. Whereas nuclear blebs were present equally throughout interphase, nuclear blebs formed predominantly in G1 and then persisted into G2. Actin-based nuclear confinement and focal adhesion density was greater in G1 versus G2 cells. Removal of focal adhesions through treatment with an inhibitor resulted in decreased nuclear confinement and blebbing, supporting this as the underlying mechanism. Upon artificial confinement, G2 nuclei ruptured more than G1 nuclei. Single nucleus micromanipulation force measurements confirmed that G1 nuclei were stiffer than G2 nuclei in both the chromatin-based and lamin-based nuclear stiffness regimes. Decreased nuclear stiffness can be explained by loss of peripheral H3K9me3 from G1 to G2, recapitulated by H3K9me3 inhibition through treatment with chaetocin. Cell cycle-based changes in nuclear and actin mechanics impact nuclear integrity and shape.<\/jats:p>","DOI":"10.1242\/jcs.264118","type":"journal-article","created":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T10:25:43Z","timestamp":1768818343000},"source":"Crossref","is-referenced-by-count":4,"title":["Changes in nuclear and actin mechanics from G1 to G2 affect nuclear integrity"],"prefix":"10.1242","volume":"139","author":[{"given":"Samantha","family":"Bunner","sequence":"first","affiliation":[{"name":"University of Massachusetts Amherst 1 Biology Department , , Amherst, MA 01003 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katie","family":"Huang","sequence":"additional","affiliation":[{"name":"University of Massachusetts Amherst 1 Biology Department , , Amherst, MA 01003 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anish","family":"Shah","sequence":"additional","affiliation":[{"name":"University of 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of Massachusetts Amherst 2 Molecular and Cellular Biology , , Amherst, MA 01003 ,","place":["USA"]},{"name":"Howard Hughes Medical Institute 3 and the Department of Biology, University of Massachusetts, Amherst, MA 01003 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katrina B.","family":"Velle","sequence":"additional","affiliation":[{"name":"University of Massachusetts Dartmouth 4 Biology Department , , North Dartmouth MA 02747 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joshua","family":"Marcus","sequence":"additional","affiliation":[{"name":"Baylor College of Medicine 5 Department of Molecular and Cellular Biology , , Houston, TX 77030 ,","place":["USA"]},{"name":"University of Colorado Boulder 6 Molecular, Cellular and Developmental Biology , , Boulder, CO 80309 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