{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T18:12:05Z","timestamp":1780337525623,"version":"3.54.1"},"reference-count":94,"publisher":"The Company of Biologists","issue":"10","license":[{"start":{"date-parts":[[2026,5,26]],"date-time":"2026-05-26T00:00:00Z","timestamp":1779753600000},"content-version":"vor","delay-in-days":11,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/100000053","name":"National Eye Institute","doi-asserted-by":"crossref","award":["R01 EY017724"],"award-info":[{"award-number":["R01 EY017724"]}],"id":[{"id":"10.13039\/100000053","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100006094","name":"University of Delaware","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100006094","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100017014","name":"Delaware Biotechnology Institute","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100017014","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["NIGMS S10OD028725"],"award-info":[{"award-number":["NIGMS S10OD028725"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["NIH\/NIGMS P20GM103446"],"award-info":[{"award-number":["NIH\/NIGMS P20GM103446"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,5,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Cyclase-associated protein 2 (CAP2) is a conserved actin-binding protein that promotes actin filament (F-actin) turnover by disassembling ADF\/cofilin-decorated filaments, supporting F-actin remodeling in differentiating cells and tissues. In the ocular lens, the actin cytoskeleton is crucial for maintaining tissue biomechanical properties during fiber cell maturation. To assess the role of CAP2 in the lens, we examined lens-specific CAP2 knockout (CAP2cKO) mice at cellular and tissue levels. CAP2cKO lenses were normal in size, shape and transparency but exhibited increased stiffness under compression and enhanced recovery after load removal. Although total actin and F-actin levels were unchanged, immunofluorescence revealed higher levels for tropomodulin 1 (Tmod1, F-actin pointed-end capping), tropomyosin isoform 3.5 (Tpm3.5, F-actin stabilizing) and T-plastin (also known as PLS3; F-actin bundling) in F-actin-rich membrane protrusions of mature fibers, whereas that for \u03b1-actinin-1 (F-actin cross-linking) was reduced. These findings suggest that CAP2 loss disrupts F-actin remodeling, indirectly promoting filament stabilization through Tpm3.5 binding, Tmod1 capping and T-plastin bundling. Consequently, F-actin networks become stiffer and more resilient, altering lens biomechanics. This study provides the first evidence that CAP2 maintains cell biomechanical properties in a non-muscle tissue through influencing the composition of actin cytoskeleton networks<\/jats:p>","DOI":"10.1242\/jcs.264491","type":"journal-article","created":{"date-parts":[[2026,5,26]],"date-time":"2026-05-26T13:23:13Z","timestamp":1779801793000},"source":"Crossref","is-referenced-by-count":1,"title":["Loss of cyclase-associated protein 2 alters actin cytoskeleton organization and biomechanical properties of the ocular lens"],"prefix":"10.1242","volume":"139","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-5691-7867","authenticated-orcid":false,"given":"Sepideh","family":"Cheheltani","sequence":"first","affiliation":[{"name":"University of Delaware Department of Biological Sciences , , Newark, DE 19716 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0223-8170","authenticated-orcid":false,"given":"Megan","family":"Coffin","sequence":"additional","affiliation":[{"name":"University of Delaware Department of Biological Sciences , , Newark, DE 19716 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3161-8802","authenticated-orcid":false,"given":"Velia M.","family":"Fowler","sequence":"additional","affiliation":[{"name":"University of Delaware Department of Biological Sciences , , Newark, DE 19716 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2026,5,26]]},"reference":[{"key":"2026052609230611200_JCS264491C1","doi-asserted-by":"publisher","first-page":"41","DOI":"10.1016\/j.exer.2016.03.016","article-title":"The molecular mechanisms underlying lens fiber elongation","volume":"156","author":"Audette","year":"2017","journal-title":"Exp. Eye Res."},{"key":"2026052609230611200_JCS264491C2","doi-asserted-by":"publisher","first-page":"626","DOI":"10.1002\/jcb.20062","article-title":"ERM proteins of the lens","volume":"92","author":"Bagchi","year":"2004","journal-title":"J. Cell. Biochem."},{"key":"2026052609230611200_JCS264491C3","doi-asserted-by":"publisher","first-page":"62","DOI":"10.3410\/B2-62","article-title":"Roles of ADF\/cofilin in actin polymerization and beyond","volume":"2","author":"Bamburg","year":"2010","journal-title":"F1000 Biol. Rep."},{"key":"2026052609230611200_JCS264491C4","doi-asserted-by":"publisher","first-page":"37","DOI":"10.1083\/jcb.137.1.37","article-title":"Chromatin degradation in differentiating fiber cells of the eye lens","volume":"137","author":"Bassnett","year":"1997","journal-title":"J. Cell Biol."},{"key":"2026052609230611200_JCS264491C5","doi-asserted-by":"publisher","first-page":"181","DOI":"10.1016\/j.preteyeres.2017.04.001","article-title":"The lens growth process","volume":"60","author":"Bassnett","year":"2017","journal-title":"Prog. Retin. Eye Res."},{"key":"2026052609230611200_JCS264491C6","doi-asserted-by":"publisher","first-page":"2155","DOI":"10.1242\/jcs.112.13.2155","article-title":"Molecular architecture of the lens fiber cell basal membrane complex","volume":"112","author":"Bassnett","year":"1999","journal-title":"J. Cell Sci."},{"key":"2026052609230611200_JCS264491C7","doi-asserted-by":"publisher","first-page":"3269","DOI":"10.1167\/iovs.07-0109","article-title":"Structural specializations emerging late in mouse lens fiber cell differentiation","volume":"48","author":"Blankenship","year":"2007","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"2026052609230611200_JCS264491C8","doi-asserted-by":"publisher","first-page":"6849","DOI":"10.1073\/pnas.78.11.6849","article-title":"Fimbrin is a cytoskeletal protein that crosslinks F-actin in vitro","volume":"78","author":"Bretscher","year":"1981","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2026052609230611200_JCS264491C92","doi-asserted-by":"publisher","first-page":"3011","DOI":"10.1242\/jcs.110.24.3011","article-title":"Ezrin: a protein requiring conformational activation to link microfilaments to the plasma membrane in the assembly of cell surface structures","volume":"110","author":"Bretscher","year":"1997","journal-title":"J. Cell Sci."},{"key":"2026052609230611200_JCS264491C9","doi-asserted-by":"publisher","first-page":"1562583","DOI":"10.3389\/fopht.2025.1562583","article-title":"Comparative analysis of rodent lens morphometrics and biomechanical properties","volume":"5","author":"Cheheltani","year":"2025","journal-title":"Front. Ophthalmol."},{"key":"2026052609230611200_JCS264491C10","doi-asserted-by":"publisher","first-page":"53","DOI":"10.1167\/iovs.66.2.53","article-title":"Depth and strain-dependent structural responses of mouse lens fiber cells during whole lens shape changes","volume":"66","author":"Cheheltani","year":"2025","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"2026052609230611200_JCS264491C11","doi-asserted-by":"publisher","first-page":"53986","DOI":"10.3791\/53986","article-title":"Sequential application of glass coverslips to assess the compressive stiffness of the mouse lens: strain and morphometric analyses","volume":"111","author":"Cheng","year":"2016","journal-title":"J. Vis. Exp"},{"key":"2026052609230611200_JCS264491C12","doi-asserted-by":"publisher","first-page":"4084","DOI":"10.1167\/iovs.16-19949","article-title":"Tropomodulin 1 regulation of actin is required for the formation of large paddle protrusions between mature lens fiber cells","volume":"57","author":"Cheng","year":"2016","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"2026052609230611200_JCS264491C13","doi-asserted-by":"publisher","first-page":"58","DOI":"10.1016\/j.exer.2016.03.005","article-title":"The lens actin filament cytoskeleton: Diverse structures for complex functions","volume":"156","author":"Cheng","year":"2017","journal-title":"Exp. Eye Res."},{"key":"2026052609230611200_JCS264491C14","doi-asserted-by":"publisher","first-page":"jcs222042","DOI":"10.1242\/jcs.222042","article-title":"Tropomyosin 3.5 protects the F-actin networks required for tissue biomechanical properties","volume":"131","author":"Cheng","year":"2018","journal-title":"J. Cell Sci."},{"key":"2026052609230611200_JCS264491C15","doi-asserted-by":"publisher","first-page":"12497","DOI":"10.18632\/aging.102584","article-title":"Age-related changes in eye lens biomechanics, morphology, refractive index and transparency","volume":"11","author":"Cheng","year":"2019","journal-title":"Aging (Albany NY)"},{"key":"2026052609230611200_JCS264491C16","doi-asserted-by":"publisher","first-page":"365","DOI":"10.1038\/s42003-021-01893-w","article-title":"CAP2 is a regulator of actin pointed end dynamics and myofibrillogenesis in cardiac muscle","volume":"4","author":"Colpan","year":"2021","journal-title":"Commun. Biol."},{"key":"2026052609230611200_JCS264491C17","doi-asserted-by":"publisher","first-page":"151","DOI":"10.1016\/j.exer.2008.08.002","article-title":"The lens capsule","volume":"88","author":"Danysh","year":"2009","journal-title":"Exp. Eye Res."},{"key":"2026052609230611200_JCS264491C18","doi-asserted-by":"publisher","first-page":"35","DOI":"10.1016\/j.yexcr.2012.09.013","article-title":"Involvement of hepatocellular carcinoma biomarker, cyclase-associated protein 2 in zebrafish body development and cancer progression","volume":"319","author":"Effendi","year":"2013","journal-title":"Exp. Cell Res."},{"key":"2026052609230611200_JCS264491C19","doi-asserted-by":"publisher","first-page":"10.3791\/66017","DOI":"10.3791\/66017","article-title":"Whole mount imaging methodology to visualize and quantify peripheral lens structure, cell morphology, and organization","volume":"203","author":"Emin","year":"2024","journal-title":"J. Vis. Exp"},{"key":"2026052609230611200_JCS264491C20","doi-asserted-by":"publisher","first-page":"17256","DOI":"10.1038\/srep17256","article-title":"CAP2 in cardiac conduction, sudden cardiac death and eye development","volume":"5","author":"Field","year":"2015","journal-title":"Sci. Rep."},{"key":"2026052609230611200_JCS264491C21","first-page":"166","article-title":"Tropomodulin and tropomyosin mediate lens cell actin cytoskeleton reorganization in vitro","volume":"41","author":"Fischer","year":"2000","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"2026052609230611200_JCS264491C22","doi-asserted-by":"publisher","first-page":"3860","DOI":"10.1167\/iovs.10-6231","article-title":"Intermediate filaments regulate tissue size and stiffness in the murine lens","volume":"52","author":"Fudge","year":"2011","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"2026052609230611200_JCS264491C23","doi-asserted-by":"publisher","first-page":"e48734","DOI":"10.1371\/journal.pone.0048734","article-title":"Tmod1 and CP49 synergize to control the fiber cell geometry, transparency, and mechanical stiffness of the mouse lens","volume":"7","author":"Gokhin","year":"2012","journal-title":"PLoS ONE"},{"key":"2026052609230611200_JCS264491C24","doi-asserted-by":"publisher","first-page":"1337","DOI":"10.1016\/j.jmb.2004.07.076","article-title":"Aquaporin-0 membrane junctions form upon proteolytic cleavage","volume":"342","author":"Gonen","year":"2004","journal-title":"J. Mol. Biol."},{"key":"2026052609230611200_JCS264491C25","doi-asserted-by":"publisher","first-page":"1007","DOI":"10.1007\/s00439-019-01995-w","article-title":"Mouse models for microphthalmia, anophthalmia and cataracts","volume":"138","author":"Graw","year":"2019","journal-title":"Hum. Genet."},{"key":"2026052609230611200_JCS264491C26","doi-asserted-by":"publisher","first-page":"4021","DOI":"10.1167\/iovs.18-26270","article-title":"Connexin 50 and AQP0 are essential in maintaining organization and integrity of lens fibers","volume":"60","author":"Gu","year":"2019","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"2026052609230611200_JCS264491C27","doi-asserted-by":"publisher","first-page":"2965","DOI":"10.1242\/jcs.172502","article-title":"Tropomyosin \u2013 master regulator of actin filament function in the cytoskeleton","volume":"128","author":"Gunning","year":"2015","journal-title":"J. Cell Sci."},{"key":"2026052609230611200_JCS264491C28","doi-asserted-by":"publisher","first-page":"46","DOI":"10.1186\/s40662-023-00365-y","article-title":"Age-related changes in geometry and transparency of human crystalline lens revealed by optical signal discontinuity zones in swept-source OCT images","volume":"10","author":"Gupta","year":"2023","journal-title":"Eye Vis. (Lond)"},{"key":"2026052609230611200_JCS264491C29","doi-asserted-by":"publisher","first-page":"212","DOI":"10.1002\/aja.1001830304","article-title":"Synthesis of lens capsule and plasma membrane glycoproteins by lens epithelial cells and fibers in the rat","volume":"183","author":"Haddad","year":"1988","journal-title":"Am. J. Anat."},{"key":"2026052609230611200_JCS264491C30","doi-asserted-by":"publisher","first-page":"2633004021997447","DOI":"10.1177\/2633004021997447","article-title":"Animal and cellular models of microphthalmia","volume":"2","author":"Harding","year":"2021","journal-title":"Ther. Adv. Rare Dis."},{"key":"2026052609230611200_JCS264491C31","doi-asserted-by":"publisher","first-page":"558","DOI":"10.1007\/s00018-022-04593-8","article-title":"Functional interdependence of the actin regulators CAP1 and cofilin1 in control of dendritic spine morphology","volume":"79","author":"Heinze","year":"2022","journal-title":"Cell. Mol. Life Sci."},{"key":"2026052609230611200_JCS264491C93","doi-asserted-by":"publisher","first-page":"649","DOI":"10.1091\/mbc.e04-07-0555","article-title":"Actin-depolymerizing factor and cofilin-1 play overlapping roles in promoting rapid F-actin depolymerization in mammalian nonmuscle cells","volume":"16","author":"Hotulainen","year":"2005","journal-title":"Mol. Biol. Cell"},{"key":"2026052609230611200_JCS264491C32","doi-asserted-by":"publisher","first-page":"20","DOI":"10.1167\/iovs.64.4.20","article-title":"Nonmuscle myosin IIA regulates the precise alignment of hexagonal eye lens epithelial cells during fiber cell formation and differentiation","volume":"64","author":"Islam","year":"2023","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"2026052609230611200_JCS264491C33","doi-asserted-by":"publisher","first-page":"789","DOI":"10.1002\/cm.21853","article-title":"Disease-related non-muscle myosin IIA D1424N rod domain mutation, but not R702C motor domain mutation, disrupts mouse ocular lens fiber cell alignment and hexagonal packing","volume":"81","author":"Islam","year":"2024","journal-title":"Cytoskeleton (Hoboken)"},{"key":"2026052609230611200_JCS264491C34","doi-asserted-by":"publisher","first-page":"165","DOI":"10.1111\/j.1365-2567.2004.01882.x","article-title":"Ezrin\/radixin\/moesin proteins and Rho GTPase signalling in leucocytes","volume":"112","author":"Ivetic","year":"2004","journal-title":"Immunology"},{"key":"2026052609230611200_JCS264491C35","doi-asserted-by":"publisher","first-page":"708","DOI":"10.1016\/j.tcb.2021.06.006","article-title":"Redefining actin dynamics of the pointed-end complex in striated muscle","volume":"31","author":"Iwanski","year":"2021","journal-title":"Trends Cell Biol."},{"key":"2026052609230611200_JCS264491C36","doi-asserted-by":"publisher","first-page":"671","DOI":"10.1091\/mbc.E18-12-0815","article-title":"Tropomyosin isoforms differentially tune actin filament length and disassembly","volume":"30","author":"Jansen","year":"2019","journal-title":"Mol. Biol. Cell"},{"key":"2026052609230611200_JCS264491C37","doi-asserted-by":"publisher","first-page":"100028","DOI":"10.1016\/j.jlr.2021.100028","article-title":"Generation and validation of a conditional knockout mouse model for desmosterolosis","volume":"62","author":"Kanuri","year":"2021","journal-title":"J. Lipid Res."},{"key":"2026052609230611200_JCS264491C38","doi-asserted-by":"publisher","first-page":"2531","DOI":"10.1167\/iovs.07-1443","article-title":"In vivo study of changes in refractive index distribution in the human crystalline lens with age and accommodation","volume":"49","author":"Kasthurirangan","year":"2008","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"2026052609230611200_JCS264491C39","doi-asserted-by":"publisher","first-page":"8397","DOI":"10.1073\/pnas.1813351116","article-title":"CAP2 deficiency delays myofibril actin cytoskeleton differentiation and disturbs skeletal muscle architecture and function","volume":"116","author":"Kepser","year":"2019","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2026052609230611200_JCS264491C40","doi-asserted-by":"publisher","first-page":"4789","DOI":"10.1038\/s41598-021-84213-w","article-title":"Cyclase-associated protein 2 (CAP2) controls MRTF-A localization and SRF activity in mouse embryonic fibroblasts","volume":"11","author":"Kepser","year":"2021","journal-title":"Sci. Rep."},{"key":"2026052609230611200_JCS264491C41","doi-asserted-by":"publisher","first-page":"1272","DOI":"10.1364\/BOE.9.001272","article-title":"Change in human lens dimensions, lens refractive index distribution and ciliary body ring diameter with accommodation","volume":"9","author":"Khan","year":"2018","journal-title":"Biomed. Opt. Express"},{"key":"2026052609230611200_JCS264491C42","first-page":"1527","article-title":"Membrane interlocking domains in the lens","volume":"27","author":"Kistler","year":"1986","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"2026052609230611200_JCS264491C43","doi-asserted-by":"publisher","first-page":"32","DOI":"10.1016\/j.ejcb.2014.10.004","article-title":"CAP2 is a regulator of the actin cytoskeleton and its absence changes infiltration of inflammatory cells and contraction of wounds","volume":"94","author":"Kosmas","year":"2015","journal-title":"Eur. J. Cell Biol."},{"key":"2026052609230611200_JCS264491C44","doi-asserted-by":"publisher","first-page":"283","DOI":"10.1007\/978-0-387-85766-4_21","article-title":"Tropomodulin\/tropomyosin interactions regulate actin pointed end dynamics","volume":"644","author":"Kostyukova","year":"2008","journal-title":"Adv. Exp. Med. Biol."},{"key":"2026052609230611200_JCS264491C45","doi-asserted-by":"publisher","first-page":"1892","DOI":"10.1038\/s41467-018-04231-7","article-title":"Structural basis of actin monomer re-charging by cyclase-associated protein","volume":"9","author":"Kotila","year":"2018","journal-title":"Nat. Commun."},{"key":"2026052609230611200_JCS264491C46","doi-asserted-by":"publisher","first-page":"5320","DOI":"10.1038\/s41467-019-13213-2","article-title":"Mechanism of synergistic actin filament pointed end depolymerization by cyclase-associated protein and cofilin","volume":"10","author":"Kotila","year":"2019","journal-title":"Nat. Commun."},{"key":"2026052609230611200_JCS264491C47","doi-asserted-by":"publisher","first-page":"180","DOI":"10.3389\/fncel.2016.00180","article-title":"Neuronal actin dynamics, spine density and neuronal dendritic complexity are regulated by CAP2","volume":"10","author":"Kumar","year":"2016","journal-title":"Front. Cell Neurosci."},{"key":"2026052609230611200_JCS264491C48","doi-asserted-by":"publisher","first-page":"1617","DOI":"10.1167\/iovs.11-8839","article-title":"iSyTE: integrated Systems Tool for Eye gene discovery","volume":"53","author":"Lachke","year":"2012","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"2026052609230611200_JCS264491C49","doi-asserted-by":"publisher","first-page":"10","DOI":"10.1186\/s40246-019-0192-8","article-title":"Considerations for the use of Cre recombinase for conditional gene deletion in the mouse lens","volume":"13","author":"Lam","year":"2019","journal-title":"Hum. Genomics"},{"key":"2026052609230611200_JCS264491C50","doi-asserted-by":"publisher","first-page":"jcs259356","DOI":"10.1242\/jcs.259356","article-title":"Mechanical role of the submembrane spectrin scaffold in red blood cells and neurons","volume":"135","author":"Leterrier","year":"2022","journal-title":"J. Cell Sci."},{"key":"2026052609230611200_JCS264491C51","doi-asserted-by":"publisher","first-page":"11","DOI":"10.1167\/tvst.13.7.11","article-title":"Construction and identification of a novel mice model of microphthalmia","volume":"13","author":"Li","year":"2024","journal-title":"Transl. Vis. Sci. Technol."},{"key":"2026052609230611200_JCS264491C52","doi-asserted-by":"publisher","first-page":"1562","DOI":"10.1167\/iovs.05-1313","article-title":"The C terminus of lens aquaporin 0 interacts with the cytoskeletal proteins filensin and CP49","volume":"47","author":"Lindsey Rose","year":"2006","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"2026052609230611200_JCS264491C53","doi-asserted-by":"publisher","first-page":"305","DOI":"10.1016\/S0248-4900(00)01078-9","article-title":"ERM proteins: from cellular architecture to cell signaling","volume":"92","author":"Louvet-Vall\u00e9e","year":"2000","journal-title":"Biol. Cell"},{"key":"2026052609230611200_JCS264491C54","doi-asserted-by":"crossref","DOI":"10.1017\/CBO9780511529825","volume-title":"Development of the Ocular Lens","author":"Lovicu","year":"2004"},{"key":"2026052609230611200_JCS264491C55","doi-asserted-by":"publisher","first-page":"179","DOI":"10.1016\/j.ydbio.2011.06.036","article-title":"Periaxin is required for hexagonal geometry and membrane organization of mature lens fibers","volume":"357","author":"Maddala","year":"2011","journal-title":"Dev. Biol."},{"key":"2026052609230611200_JCS264491C56","doi-asserted-by":"publisher","first-page":"984","DOI":"10.1074\/jbc.M112.435719","article-title":"Mammalian and malaria parasite cyclase-associated proteins catalyze nucleotide exchange on G-actin through a conserved mechanism","volume":"288","author":"Makkonen","year":"2013","journal-title":"J. Biol. Chem."},{"key":"2026052609230611200_JCS264491C57","doi-asserted-by":"publisher","first-page":"165","DOI":"10.1006\/scel.1994.1021","article-title":"Actin crosslinking proteins at the leading edge","volume":"5","author":"Matsudaira","year":"1994","journal-title":"Semin. Cell Biol."},{"key":"2026052609230611200_JCS264491C58","doi-asserted-by":"publisher","first-page":"e2205370119","DOI":"10.1073\/pnas.2205370119","article-title":"Structural mechanism for bidirectional actin cross-linking by T-plastin","volume":"119","author":"Mei","year":"2022","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2026052609230611200_JCS264491C59","doi-asserted-by":"publisher","first-page":"1134086","DOI":"10.3389\/fbioe.2023.1134086","article-title":"The lens capsule significantly affects the viscoelastic properties of the lens as quantified by optical coherence elastography","volume":"11","author":"Mekonnen","year":"2023","journal-title":"Front. Bioeng. Biotechnol."},{"key":"2026052609230611200_JCS264491C60","doi-asserted-by":"publisher","first-page":"915","DOI":"10.1083\/jcb.200905065","article-title":"Tropomodulin1 is required for membrane skeleton organization and hexagonal geometry of fiber cells in the mouse lens","volume":"186","author":"Nowak","year":"2009","journal-title":"J. Cell Biol."},{"key":"2026052609230611200_JCS264491C61","doi-asserted-by":"publisher","first-page":"3249","DOI":"10.1242\/jcs.128231","article-title":"The role of cyclase-associated protein in regulating actin filament dynamics - more than a monomer-sequestration factor","volume":"126","author":"Ono","year":"2013","journal-title":"J. Cell Sci."},{"key":"2026052609230611200_JCS264491C62","doi-asserted-by":"publisher","first-page":"1065","DOI":"10.1083\/jcb.200110013","article-title":"Tropomyosin inhibits ADF\/cofilin-dependent actin filament dynamics","volume":"156","author":"Ono","year":"2002","journal-title":"J. Cell Biol."},{"key":"2026052609230611200_JCS264491C63","doi-asserted-by":"publisher","first-page":"1963","DOI":"10.1091\/mbc.E18-01-0035","article-title":"The effects of mechanical strain on mouse eye lens capsule and cellular microstructure","volume":"29","author":"Parreno","year":"2018","journal-title":"Mol. Biol. Cell"},{"key":"2026052609230611200_JCS264491C64","doi-asserted-by":"publisher","first-page":"2","DOI":"10.1167\/iovs.61.6.2","article-title":"Tropomyosin 3.1 association with actin stress fibers is required for lens epithelial to mesenchymal transition","volume":"61","author":"Parreno","year":"2020","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"2026052609230611200_JCS264491C65","doi-asserted-by":"publisher","first-page":"2702","DOI":"10.1007\/s00018-007-7316-3","article-title":"CAP2, cyclase-associated protein 2, is a dual compartment protein","volume":"64","author":"Peche","year":"2007","journal-title":"Cell. Mol. Life Sci."},{"key":"2026052609230611200_JCS264491C66","doi-asserted-by":"publisher","first-page":"527","DOI":"10.1007\/s00018-012-1142-y","article-title":"Ablation of cyclase-associated protein 2 (CAP2) leads to cardiomyopathy","volume":"70","author":"Peche","year":"2013","journal-title":"Cell. Mol. Life Sci."},{"key":"2026052609230611200_JCS264491C67","doi-asserted-by":"publisher","first-page":"fcaa086","DOI":"10.1093\/braincomms\/fcaa086","article-title":"Cyclase-associated protein 2 dimerization regulates cofilin in synaptic plasticity and Alzheimer's disease","volume":"2","author":"Pelucchi","year":"2020","journal-title":"Brain Commun."},{"key":"2026052609230611200_JCS264491C68","doi-asserted-by":"publisher","first-page":"10923","DOI":"10.1074\/jbc.M808760200","article-title":"Reconstitution and dissection of the 600-kDa Srv2\/CAP complex: roles for oligomerization and cofilin-actin binding in driving actin turnover","volume":"284","author":"Quintero-Monzon","year":"2009","journal-title":"J. Biol. Chem."},{"key":"2026052609230611200_JCS264491C69","doi-asserted-by":"publisher","first-page":"698","DOI":"10.1016\/j.semcdb.2006.10.011","article-title":"The role of the lens actin cytoskeleton in fiber cell elongation and differentiation","volume":"17","author":"Rao","year":"2006","journal-title":"Semin. Cell Dev. Biol."},{"key":"2026052609230611200_JCS264491C70","doi-asserted-by":"publisher","first-page":"508","DOI":"10.1016\/j.bbrc.2008.01.128","article-title":"Characterization of lens fiber cell triton insoluble fraction reveals ERM (ezrin, radixin, moesin) proteins as major cytoskeletal-associated proteins","volume":"368","author":"Rao","year":"2008","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"2026052609230611200_JCS264491C71","doi-asserted-by":"publisher","first-page":"20230316","DOI":"10.1098\/rsif.2023.0316","article-title":"The effect of fibre cell remodelling on the power and optical quality of the lens","volume":"20","author":"Rodriguez","year":"2023","journal-title":"J. R Soc. Interface"},{"key":"2026052609230611200_JCS264491C72","doi-asserted-by":"publisher","first-page":"586631","DOI":"10.3389\/fcell.2020.586631","article-title":"CAPt'n of actin dynamics: recent advances in the molecular, developmental and physiological functions of cyclase-associated protein (CAP)","volume":"8","author":"Rust","year":"2020","journal-title":"Front. Cell Dev. Biol."},{"key":"2026052609230611200_JCS264491C73","doi-asserted-by":"publisher","first-page":"5319","DOI":"10.1038\/s41467-019-13268-1","article-title":"Synergy between Cyclase-associated protein and Cofilin accelerates actin filament depolymerization by two orders of magnitude","volume":"10","author":"Shekhar","year":"2019","journal-title":"Nat. Commun."},{"key":"2026052609230611200_JCS264491C74","doi-asserted-by":"publisher","first-page":"15","DOI":"10.1016\/j.jtbi.2015.03.021","article-title":"A stochastic model of eye lens growth","volume":"376","author":"\u0160iki\u0107","year":"2015","journal-title":"J. Theor. Biol."},{"key":"2026052609230611200_JCS264491C75","doi-asserted-by":"publisher","first-page":"160695","DOI":"10.1098\/rsos.160695","article-title":"A full lifespan model of vertebrate lens growth","volume":"4","author":"\u0160iki\u0107","year":"2017","journal-title":"R. Soc. Open Sci."},{"key":"2026052609230611200_JCS264491C76","doi-asserted-by":"publisher","first-page":"339","DOI":"10.1016\/j.bbrc.2015.04.138","article-title":"Role of Aquaporin 0 in lens biomechanics","volume":"462","author":"Sindhu Kumari","year":"2015","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"2026052609230611200_JCS264491C77","doi-asserted-by":"publisher","first-page":"337","DOI":"10.1038\/nature10163","article-title":"A conditional knockout resource for the genome\u2013wide study of mouse gene function","volume":"474","author":"Skarnes","year":"2011","journal-title":"Nature"},{"key":"2026052609230611200_JCS264491C78","doi-asserted-by":"publisher","first-page":"4985","DOI":"10.1242\/jcs.00815","article-title":"A novel cell-cell junction system: the cortex adhaerens mosaic of lens fiber cells","volume":"116","author":"Straub","year":"2003","journal-title":"J. Cell Sci."},{"key":"2026052609230611200_JCS264491C79","doi-asserted-by":"publisher","first-page":"47","DOI":"10.1186\/1750-1172-2-47","article-title":"Anophthalmia and microphthalmia","volume":"2","author":"Verma","year":"2007","journal-title":"Orphanet J. Rare Dis."},{"key":"2026052609230611200_JCS264491C80","doi-asserted-by":"publisher","first-page":"35437","DOI":"10.1074\/jbc.M113.505669","article-title":"Interactome analysis reveals ezrin can adopt multiple conformational states","volume":"288","author":"Viswanatha","year":"2013","journal-title":"J. Biol. Chem."},{"key":"2026052609230611200_JCS264491C81","doi-asserted-by":"publisher","first-page":"10.3791\/65638","DOI":"10.3791\/65638","article-title":"Preparation and immunofluorescence staining of bundles and single fiber cells from the cortex and nucleus of the eye lens","volume":"196","author":"Vu","year":"2023","journal-title":"J. Vis. Exp"},{"key":"2026052609230611200_JCS264491C82","doi-asserted-by":"publisher","first-page":"114","DOI":"10.1016\/j.preteyeres.2018.11.004","article-title":"Biomechanics of the human lens and accommodative system: Functional relevance to physiological states","volume":"71","author":"Wang","year":"2019","journal-title":"Prog. Retin. Eye Res."},{"key":"2026052609230611200_JCS264491C83","doi-asserted-by":"publisher","first-page":"5079","DOI":"10.1167\/iovs.10-6998","article-title":"Aquaporin-0 interacts with the FERM domain of ezrin\/radixin\/moesin proteins in the ocular lens","volume":"52","author":"Wang","year":"2011","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"2026052609230611200_JCS264491C84","doi-asserted-by":"publisher","first-page":"1627","DOI":"10.1083\/jcb.127.6.1627","article-title":"Tropomodulin caps the pointed ends of actin filaments","volume":"127","author":"Weber","year":"1994","journal-title":"J. Cell Biol."},{"key":"2026052609230611200_JCS264491C85","doi-asserted-by":"publisher","first-page":"36","DOI":"10.1016\/j.exer.2012.01.011","article-title":"Shear modulus data for the human lens determined from a spinning lens test","volume":"97","author":"Wilde","year":"2012","journal-title":"Exp. Eye Res."},{"key":"2026052609230611200_JCS264491C86","doi-asserted-by":"publisher","first-page":"2697","DOI":"10.1016\/j.cub.2016.07.080","article-title":"Fascin- and \u03b1-actinin-bundled networks contain intrinsic structural features that drive protein sorting","volume":"26","author":"Winkelman","year":"2016","journal-title":"Curr. Biol."},{"key":"2026052609230611200_JCS264491C87","doi-asserted-by":"publisher","first-page":"1359","DOI":"10.1242\/jcs.107.5.1359","article-title":"Identification and characterization of tropomodulin and tropomyosin in the adult rat lens","volume":"107","author":"Woo","year":"1994","journal-title":"J. Cell Sci."},{"key":"2026052609230611200_JCS264491C94","doi-asserted-by":"publisher","first-page":"337","DOI":"10.1002\/cm.21031","article-title":"Tropomodulins: pointed-end capping proteins that regulate actin filament architecture in diverse cell types","volume":"69","author":"Yamashiro","year":"2012","journal-title":"Cytoskeleton"},{"key":"2026052609230611200_JCS264491C88","doi-asserted-by":"publisher","first-page":"631","DOI":"10.1083\/jcb.103.2.631","article-title":"Intracellular localization of the 55-kD actin-bundling protein in cultured cells: spatial relationships with actin, alpha-actinin, tropomyosin, and fimbrin","volume":"103","author":"Yamashiro-Matsumura","year":"1986","journal-title":"J. Cell Biol."},{"key":"2026052609230611200_JCS264491C89","doi-asserted-by":"publisher","first-page":"569","DOI":"10.14348\/molcells.2021.0031","article-title":"Endoplasmic reticulum stress induces CAP2 expression promoting epithelial-mesenchymal transition in liver cancer cells","volume":"44","author":"Yoon","year":"2021","journal-title":"Mol. Cells"},{"key":"2026052609230611200_JCS264491C90","doi-asserted-by":"publisher","first-page":"435","DOI":"10.3390\/ijms21020435","article-title":"Ezrin phosphorylation at T567 modulates cell migration, mechanical properties, and cytoskeletal organization","volume":"21","author":"Zhang","year":"2020","journal-title":"Int. J. Mol. Sci."},{"key":"2026052609230611200_JCS264491C91","doi-asserted-by":"publisher","first-page":"1930","DOI":"10.1167\/iovs.03-0856","article-title":"Insertion of a Pax6 consensus binding site into the \u03b1A-crystallin promoter acts as a lens epithelial cell enhancer in transgenic mice","volume":"45","author":"Zhao","year":"2004","journal-title":"Invest. Ophthalmol. Vis. Sci."}],"container-title":["Journal of Cell Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.biologists.com\/jcs\/article-pdf\/doi\/10.1242\/jcs.264491\/3700930\/jcs264491.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/journals.biologists.com\/jcs\/article-pdf\/doi\/10.1242\/jcs.264491\/3700930\/jcs264491.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,26]],"date-time":"2026-05-26T13:23:21Z","timestamp":1779801801000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.biologists.com\/jcs\/article\/139\/10\/jcs264491\/371809\/Loss-of-cyclase-associated-protein-2-alters-actin"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5,15]]},"references-count":94,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2026,5,15]]}},"URL":"https:\/\/doi.org\/10.1242\/jcs.264491","relation":{},"ISSN":["0021-9533","1477-9137"],"issn-type":[{"value":"0021-9533","type":"print"},{"value":"1477-9137","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5,15]]},"article-number":"jcs264491"}}