{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T09:18:10Z","timestamp":1788513490046,"version":"build-2803163510"},"reference-count":0,"publisher":"The Company of Biologists","license":[{"start":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T00:00:00Z","timestamp":1788480000000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"crossref","award":["23KJ1926, 24K18354"],"award-info":[{"award-number":["23KJ1926, 24K18354"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"crossref","award":["22J12056, 23K14433"],"award-info":[{"award-number":["22J12056, 23K14433"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"crossref","award":["17K08521, 22K06799"],"award-info":[{"award-number":["17K08521, 22K06799"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>The glomerular capillary system is essential for efficient glomerular filtration; however, the morphogenetic processes underlying its development remain incompletely understood. In the present study, we employed two volume EM modalities\u2014array tomography and FIB-SEM tomography\u2014to perform 3D reconstruction of the glomerular capillary system at successive developmental stages in neonatal rats. The results revealed that glomerular capillary development proceeds through four morphologically distinct phases: primitive glomerular capillaries, initial capillary loops, maturing capillary loops, and fully matured capillary loops. Importantly, the glomerular capillary system was found to be established through the combined action of sprouting and intussusceptive angiogenesis. Sprouting angiogenesis was particularly prominent during the formation of primitive glomerular capillaries and initial capillary loops, whereas intussusceptive angiogenesis continued from the S-shaped body stage onward. Furthermore, mesangial cell processes were observed traversing transcapillary pillars and anchoring to the glomerular basement membrane, suggesting an active role for mesangial cells in capillary splitting. These findings provide the first comprehensive ultrastructural description of glomerular capillary development and offer a morphological framework for investigating glomerular capillary anomalies under pathological conditions.<\/jats:p>","DOI":"10.1242\/jcs.264988","type":"journal-article","created":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T09:10:05Z","timestamp":1788513005000},"source":"Crossref","is-referenced-by-count":0,"title":["Volume EM revealed stepwise development of the glomerular capillary system through sprouting and Intussusceptive Angiogenesis"],"prefix":"10.1242","author":[{"given":"Yuto","family":"Kawasaki","sequence":"first","affiliation":[{"name":"1 Department of Anatomy and Life Structure, Juntendo University Graduate School of Medicine, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takayuki","family":"Miyaki","sequence":"additional","affiliation":[{"name":"1 Department of Anatomy and Life Structure, Juntendo University Graduate School of Medicine, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasue","family":"Hosoyamada","sequence":"additional","affiliation":[{"name":"1 Department of Anatomy and Life Structure, Juntendo University Graduate School of Medicine, Tokyo, Japan"},{"name":"2 Department of Nutrition, Faculty of Health Care Sciences, Chiba Prefectural University of Health Sciences, Chiba, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan Alejandro Oliva","family":"Trejo","sequence":"additional","affiliation":[{"name":"1 Department of Anatomy and Life Structure, Juntendo University Graduate School of Medicine, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingyuan","family":"Xu","sequence":"additional","affiliation":[{"name":"1 Department of Anatomy and Life Structure, Juntendo University Graduate School of Medicine, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Makoto","family":"Sugiura","sequence":"additional","affiliation":[{"name":"1 Department of Anatomy and Life Structure, Juntendo University Graduate School of Medicine, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keita","family":"Satoh","sequence":"additional","affiliation":[{"name":"1 Department of Anatomy and Life Structure, Juntendo University Graduate School of Medicine, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Koichiro","family":"Tsujikawa","sequence":"additional","affiliation":[{"name":"1 Department of Anatomy and Life Structure, Juntendo University Graduate School of Medicine, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takuya","family":"Omotehara","sequence":"additional","affiliation":[{"name":"1 Department of Anatomy and Life Structure, Juntendo University Graduate School of Medicine, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takako","family":"Negishi-Koga","sequence":"additional","affiliation":[{"name":"3 Department of Medicine for Orthopedics and Motor Organ, Juntendo University Graduate School of Medicine, Tokyo, Japan"},{"name":"4 Department of Community Medicine and Research for Bone and Joint Diseases, Juntendo University Graduate School of Medicine, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muneaki","family":"Ishijima","sequence":"additional","affiliation":[{"name":"3 Department of Medicine for Orthopedics and Motor Organ, Juntendo University Graduate School of Medicine, Tokyo, Japan"},{"name":"4 Department of Community Medicine and Research for Bone and Joint Diseases, Juntendo University Graduate School of Medicine, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junji","family":"Yamaguchi","sequence":"additional","affiliation":[{"name":"5 Laboratory of Morphology and Image Analysis, Research Support Center, Juntendo University Graduate School of Medicine, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soichiro","family":"Kakuta","sequence":"additional","affiliation":[{"name":"5 Laboratory of Morphology and Image Analysis, Research Support Center, Juntendo University Graduate School of Medicine, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6590-7912","authenticated-orcid":false,"given":"Koichiro","family":"Ichimura","sequence":"additional","affiliation":[{"name":"1 Department of Anatomy and Life Structure, Juntendo University Graduate School of Medicine, Tokyo, Japan"},{"name":"5 Laboratory of Morphology and Image Analysis, Research Support Center, Juntendo University Graduate School of Medicine, Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2026,9,4]]},"container-title":["Journal of Cell Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.biologists.com\/jcs\/article-pdf\/doi\/10.1242\/jcs.264988\/3715852\/jcs264988.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/journals.biologists.com\/jcs\/article-pdf\/doi\/10.1242\/jcs.264988\/3715852\/jcs264988.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T09:10:06Z","timestamp":1788513006000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.biologists.com\/jcs\/article\/doi\/10.1242\/jcs.264988\/372800\/Volume-EM-revealed-stepwise-development-of-the"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,4]]},"references-count":0,"URL":"https:\/\/doi.org\/10.1242\/jcs.264988","relation":{},"ISSN":["0021-9533","1477-9137"],"issn-type":[{"value":"0021-9533","type":"print"},{"value":"1477-9137","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,4]]},"article-number":"jcs.264988"}}