{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,16]],"date-time":"2023-11-16T05:14:42Z","timestamp":1700111682719},"reference-count":29,"publisher":"The Company of Biologists","issue":"1","license":[{"start":{"date-parts":[[1979,4,1]],"date-time":"1979-04-01T00:00:00Z","timestamp":291772800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1979,4,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>Capping, independent of metabolic energy, of surface immunoglobulin (S-Ig) on \u2018Hairy cells\u2019 from patients with Hairy cell leukaemia (HCL) is described. As controls leukaemic cells from a patient with a prolymphocytic leukaemia (PLL) and blood lymphocytes from healthy individuals were used. The specificity of the energy-independent capping of the HCL-cells as compared to the controls was tested by incubation of the cells at 4 \u00b0C in the presence of 01 M\u00b7 sodium azide with different FITC-labelled ligands.<\/jats:p>\n               <jats:p>In order to find an explanation for this phenomenon, the influence of cytochalasin B, colchicine and the combination of both drugs on capping of S-Ig and concanavalin (Con A)-receptors at 37 \u00b0C was investigated. Furthermore the effect of Con A on S-Ig capping and vice versa was studied. The results show that only S-Ig on HCL cells could form caps at 4 \u00b0C in the presence of sodium-azide. Cytochalasin B alone induced a strong inhibition of Con A capping on all 3 cell types, whereas S-Ig capping was unaffected. Colchicine alone had practically no effect. Anti-Ig inhibited subsequent patch and cap formation with Con A on both HCL cells and PLL cells, whereas Con A caps and patches were redistributed by anti-Ig on PLL cells, but not on HCL cells. Conversely, Con A could link S-Ig to other receptors, leading to inhibition of S-Ig capping at 4 \u00b0C on HCL cells and to co-capping of S-Ig at 37 \u00b0C on both cell types. In addition Con A induced redistribution of S-Ig caps. The combination of cocapping of S-Ig by Con A, followed by redistribution of the caps by FITC-anti-Ig simulated inhibition of S-Ig capping by Con A on PLL cells.<\/jats:p>\n               <jats:p>The major conclusions are: in some cases inhibition of capping may actually be caused by redistribution of caps; the energy-independent capping cannot be explained by free diffusion of S-Ig in the membrane through lack of any connexion with receptor-mobility regulating systems. It is proposed that the energy requirement of capping is needed to inactivate a specific mechanism, which restrains receptor mobility and which is non-operative in HCL cells.<\/jats:p>","DOI":"10.1242\/jcs.36.1.45","type":"journal-article","created":{"date-parts":[[2021,4,26]],"date-time":"2021-04-26T20:50:15Z","timestamp":1619470215000},"page":"45-59","source":"Crossref","is-referenced-by-count":3,"title":["Capping of surface immunoglobulin on \u2018hairy cells\u2019 is independent of energy production"],"prefix":"10.1242","volume":"36","author":[{"given":"TH. A. W.","family":"Splinter","sequence":"first","affiliation":[{"name":"The Netherlands Cancer Institute, Amsterdam, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. G.","family":"Collard","sequence":"additional","affiliation":[{"name":"The Netherlands Cancer Institute, Amsterdam, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anke","family":"De Wildt","sequence":"additional","affiliation":[{"name":"The Netherlands Cancer Institute, Amsterdam, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. H. M.","family":"Temmink","sequence":"additional","affiliation":[{"name":"The Netherlands Cancer Institute, Amsterdam, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francine","family":"D\u00e9cary","sequence":"additional","affiliation":[{"name":"The Netherlands Cancer Institute, Amsterdam, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","published-online":{"date-parts":[[1979,4,1]]},"reference":[{"key":"2023111604332595300_JOCES_36_1_45C1","first-page":"1307","article-title":"Fluidity of membrane lipids and lateral mobility of Concanavalin A receptors in the cell surface of normal lymphocytes and lymphocytes from patients with malignant lymphomas and leukemias","volume":"37","author":"Ben-Bassat","year":"1977","journal-title":"Cancer Res"},{"key":"2023111604332595300_JOCES_36_1_45C2","doi-asserted-by":"publisher","first-page":"525","DOI":"10.1083\/jcb.34.2.525","article-title":"The mechanism of action of binding of Colchicine-3H to cellular protein","volume":"34","author":"Borisy","year":"1967","journal-title":"J. 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