{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T18:30:54Z","timestamp":1771007454224,"version":"3.50.1"},"reference-count":20,"publisher":"The Company of Biologists","issue":"1","license":[{"start":{"date-parts":[[1981,4,1]],"date-time":"1981-04-01T00:00:00Z","timestamp":354931200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1981,4,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>A number of different assay methods have been used to study repair of strand breaks in DNA after exposure of cells to ionizing radiation. Use of these methods indicates that fibroblasts from patients with ataxia telangiectasia (AT), a multiform genetic disease exhibiting high sensitivity to ionizing radiation, have a normal ability to repair strand breaks in DNA. All of these methods determine the extent of breakage of DNA and the resealing of these breaks but do not provide information on restoration of DNA configuration in the nucleus.<\/jats:p>\n               <jats:p>In this report we have used a sensitive technique to investigate restoration of the 3-dimensional structure of DNA in AT lymphoblastoid cells after exposure to ionizing radiation. This technique provides a means of lysing cells using a high concentration of salt and a non-ionic detergent, giving rise to structures called nucleoids which contain nuclear RNA and DNA, are depleted in protein, and sediment in a manner characteristic of supercoiled DNA. We have shown that the degree of supercoiling is the same in control and AT lymphoblastoid cells using sedimentation in the presence of ethidium bromide. The extent of breakage after exposure of cells to y-radiation, and the rate of repair of these breaks are similar in both cell types. Rate of repair of strand breaks is dose dependent and the restructured rapidly sedimenting complex behaves similarly, on sucrose gradients containing ethidium bromide, to that extracted from unirradiated cells.<\/jats:p>","DOI":"10.1242\/jcs.48.1.383","type":"journal-article","created":{"date-parts":[[2021,4,26]],"date-time":"2021-04-26T20:54:50Z","timestamp":1619470490000},"page":"383-391","source":"Crossref","is-referenced-by-count":28,"title":["Repair of strand breaks in superhelical dna of ataxia telangiectasia lymphoblastoid cells"],"prefix":"10.1242","volume":"48","author":[{"given":"M. F.","family":"Lavin","sequence":"first","affiliation":[{"name":"University of Queensland Department of Biochemistry , , St Lucia, Brisbane, 4067, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Davidson","sequence":"additional","affiliation":[{"name":"University of Queensland Department of Biochemistry , , St Lucia, Brisbane, 4067, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","published-online":{"date-parts":[[1981,4,1]]},"reference":[{"key":"2023053021583075900_JOCES_48_1_383C1","doi-asserted-by":"publisher","first-page":"95","DOI":"10.1146\/annurev.ge.12.120178.000523","article-title":"Human disorders showing increased sensitivity to the induction of genetic damage","volume":"1a","author":"Arlett","year":"1978","journal-title":"A. Rev. Genet."},{"key":"2023053021583075900_JOCES_48_1_383C2","doi-asserted-by":"publisher","first-page":"484","DOI":"10.1038\/274484a0","article-title":"Identification of ataxia telangiectasia heterozygotes, a cancer prone population","volume":"374","author":"Chen","year":"1978","journal-title":"Nature, Land."},{"key":"2023053021583075900_JOCES_48_1_383C3","first-page":"261","article-title":"Supercoils in human DNA","volume":"19","author":"Cook","year":"1975","journal-title":"F. Cell Set\u2019."},{"key":"2023053021583075900_JOCES_48_1_383C4","doi-asserted-by":"publisher","first-page":"679","DOI":"10.1038\/263679a0","article-title":"Detection and repair of single-strand breaks in nuclear DNA","volume":"363","author":"Cook","year":"1976","journal-title":"Nature, Land."},{"key":"2023053021583075900_JOCES_48_1_383C5","first-page":"303","article-title":"Characterization of nuclear structures containing superhelical DNA","volume":"aa","author":"Cook","year":"1976","journal-title":"F. Cell Sri."},{"key":"2023053021583075900_JOCES_48_1_383C6","doi-asserted-by":"publisher","first-page":"809","DOI":"10.1073\/pnas.73.3.809","article-title":"DNA strand breaks, repair, and survival in X-irradiated mammalian cells","volume":"n","author":"Dugle","year":"1976","journal-title":"Proc. natn. Acad. Sci. U.S.A"},{"key":"2023053021583075900_JOCES_48_1_383C7","first-page":"61","article-title":"Genes which increase chromosomal instability in somatic cells and predispose to cancer","volume":"8","author":"German","year":"1972","journal-title":"Prog. med. Genet."},{"key":"2023053021583075900_JOCES_48_1_383C8","doi-asserted-by":"publisher","first-page":"3709","DOI":"10.1093\/nar\/8.16.3709","article-title":"Effect of ionizing radiation on DNA synthesis in ataxia telangiectasia cells","volume":"8","author":"Houldsworth","year":"1980","journal-title":"Nucleic Acids Res."},{"key":"2023053021583075900_JOCES_48_1_383C9","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1016\/B978-0-12-322650-1.50089-8","volume-title":"In DNA Repair Mechanisms","author":"Hutchinson","year":"1978"},{"key":"2023053021583075900_JOCES_48_1_383C10","doi-asserted-by":"publisher","first-page":"517","DOI":"10.1016\/0027-5107(70)90036-9","article-title":"Repair of X-ray damage in DNA of cultivated cells from patients having xeroderma pigmentosum","volume":"9","author":"Kleijer","year":"1970","journal-title":"Mutation Ret."},{"key":"2023053021583075900_JOCES_48_1_383C11","doi-asserted-by":"publisher","first-page":"325","DOI":"10.1016\/0090-1229(78)90188-5","article-title":"Lymphocytes in ataxia telangiectasia: Implications of the DNA repair defect","volume":"10","author":"Lavin","year":"1978","journal-title":"Clin. Immun. Immunopath."},{"key":"2023053021583075900_JOCES_48_1_383C12","doi-asserted-by":"publisher","first-page":"49","DOI":"10.1016\/0005-2787(77)90213-1","article-title":"The production and repair of double strand breaks in cells from normal humans and from patients with ataxia telangiectasia","volume":"474","author":"Lehmann","year":"1977","journal-title":"Biochim. biophys. Acta"},{"key":"2023053021583075900_JOCES_48_1_383C13","first-page":"67","volume-title":"In Biology of Radiation Carcinogenesis","author":"Ormerod","year":"1976"},{"key":"2023053021583075900_JOCES_48_1_383C14","doi-asserted-by":"publisher","first-page":"444","DOI":"10.1038\/260444a0","article-title":"Defective excision repair of y-ray-damaged DNA in human (ataxia telangiectasia) fibroblasts","volume":"260","author":"Paterson","year":"1976","journal-title":"Nature, Land."},{"key":"2023053021583075900_JOCES_48_1_383C15","doi-asserted-by":"publisher","first-page":"299","DOI":"10.1093\/nar\/4.2.299","article-title":"Single strand breakage and repair in eukaryotic DNA as assayed by Si nuclease","volume":"4","author":"Sheridan","year":"1977","journal-title":"Nucleic Acids Res."},{"key":"2023053021583075900_JOCES_48_1_383C16","doi-asserted-by":"publisher","first-page":"415","DOI":"10.1016\/0027-5107(79)90148-9","article-title":"Ataxia telangiectasia: Further considerations of the evidence for single strand break repair","volume":"61","author":"Sheridan","year":"1979","journal-title":"Mutation Res."},{"key":"2023053021583075900_JOCES_48_1_383C17","doi-asserted-by":"publisher","first-page":"407","DOI":"10.1016\/0027-5107(78)90045-3","article-title":"Unrepaired DNA strand breaks in irradiated ataxia telangiectasia lymphocytes suggested from cytogenetic observations","volume":"50","author":"Taylor","year":"1978","journal-title":"Mutation Res."},{"key":"2023053021583075900_JOCES_48_1_383C18","doi-asserted-by":"publisher","first-page":"427","DOI":"10.1038\/258427a0","article-title":"Ataxia telangiectasia: A human mutation with abnormal radiation sensitivity","volume":"ag8","author":"Taylor","year":"1975","journal-title":"Nature, Lond."},{"key":"2023053021583075900_JOCES_48_1_383C19","doi-asserted-by":"publisher","first-page":"357","DOI":"10.1016\/0027-5107(75)90211-0","article-title":"DNA strand breakage repair in ataxia telangiectasia fibroblast-like cells","volume":"33","author":"Vincent","year":"1975","journal-title":"Mutation Res."},{"key":"2023053021583075900_JOCES_48_1_383C20","doi-asserted-by":"publisher","first-page":"181","DOI":"10.1016\/B978-0-12-035405-4.50011-6","article-title":"Molecular mechanisms of radiation-induced damage to nucleic acids","volume":"5","author":"Ward","year":"1975","journal-title":"Adv. Radiat. Biol."}],"container-title":["Journal of Cell Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.biologists.com\/jcs\/article-pdf\/48\/1\/383\/2893919\/joces_48_1_383.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/journals.biologists.com\/jcs\/article-pdf\/48\/1\/383\/2893919\/joces_48_1_383.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,30]],"date-time":"2023-05-30T21:58:40Z","timestamp":1685483920000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.biologists.com\/jcs\/article\/48\/1\/383\/59080\/Repair-of-strand-breaks-in-superhelical-dna-of"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1981,4,1]]},"references-count":20,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1981,4,1]]}},"URL":"https:\/\/doi.org\/10.1242\/jcs.48.1.383","relation":{},"ISSN":["0021-9533","1477-9137"],"issn-type":[{"value":"0021-9533","type":"print"},{"value":"1477-9137","type":"electronic"}],"subject":[],"published":{"date-parts":[[1981,4,1]]}}}