{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,5]],"date-time":"2026-04-05T04:55:19Z","timestamp":1775364919140,"version":"3.50.1"},"reference-count":12,"publisher":"The Company of Biologists","issue":"1","license":[{"start":{"date-parts":[[1985,8,1]],"date-time":"1985-08-01T00:00:00Z","timestamp":491702400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1985,8,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>The immunofluorescence patterns for actin, myosin, calmodulin and tubulin were observed in Euglena gracilis Klebs strain Z during the biological clock-controlled shape changes observed with division-synchronized cells, and during two shock responses that induce cell rounding. The fluorescence patterns for actin, myosin, calmodulin and tubulin show a high degree of coincidence and are visualized as lines running parallel to, and having the same spacing as, the pellicle strips beneath the plasma membrane. The fluorescence patterns remain intact during the daily shape changes, implying that the shape changes do not result from cycles of polymerization and depolymerization of the microtubules and microfilaments. Resuspension of cells in Ca2+-free medium induces cell rounding of many of the cells. The actin and calmodulin patterns are partially disrupted by the Ca2+-free resuspension, while the myosin pattern is almost totally disrupted. Microtubules are unaffected by this treatment. Prior exposure of cells to the calmodulin antagonist trifluoperazine or to the microfilament-stabilizing peptide phalloidin stabilize the actin, myosin and calmodulin patterns against disruption by the Ca -free resuspension and other shock responses. The possibility of an actomyosin contractile system controlled by calmodulin is discussed.<\/jats:p>","DOI":"10.1242\/jcs.77.1.197","type":"journal-article","created":{"date-parts":[[2021,4,24]],"date-time":"2021-04-24T05:29:00Z","timestamp":1619242140000},"page":"197-208","source":"Crossref","is-referenced-by-count":38,"title":["Regulation of cell shape in <i>Euglena gracilis<\/i>. IV. localization of actin, myosin and calmodulin"],"prefix":"10.1242","volume":"77","author":[{"given":"Thomas A.","family":"Lonergan","sequence":"first","affiliation":[{"name":"University of New Orieans-Lakefront Department of Biological Sciences , , New Orleans, Louisiana 70148, U.SA."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","published-online":{"date-parts":[[1985,8,1]]},"reference":[{"key":"2023060116563918900_JOCES_77_1_197C1","doi-asserted-by":"publisher","first-page":"934","DOI":"10.1083\/jcb.98.3.934","article-title":"Relationship of actin and tubulin distribution to bud growth in wild-type and morphogenetic-mutant Sacchammyces cerevisiae","volume":"98","author":"Adams","year":"1984","journal-title":"F. Cell Biol"},{"key":"2023060116563918900_JOCES_77_1_197C2","doi-asserted-by":"publisher","first-page":"1973","DOI":"10.1083\/jcb.98.6.1973","article-title":"Epithelial cytoskeletal framework and nuclear matrix-intermediate filament scaffold: three-dimensional organization and protein composition","volume":"98","author":"Fey","year":"1984","journal-title":"F. Cell Biol"},{"key":"2023060116563918900_JOCES_77_1_197C3","doi-asserted-by":"publisher","first-page":"922","DOI":"10.1083\/jcb.98.3.922","article-title":"Structural rearrangements of tubulin and actin during the cell cycle of the yeast Saccaharomyces","volume":"98","author":"Kilmartin","year":"1984","journal-title":"F. Cell Biol"},{"key":"2023060116563918900_JOCES_77_1_197C4","doi-asserted-by":"publisher","first-page":"219","DOI":"10.1242\/jcs.74.1.219","article-title":"Regulation of cell shape in Euglena gracilis. III. Involvement of stable microtubules","volume":"74","author":"Lachney","year":"1985","journal-title":"F. Cell Sci"},{"key":"2023060116563918900_JOCES_77_1_197C5","first-page":"211","article-title":"Localization of cytoskeletal protein in preimplantation mouse embryos","volume":"55","author":"Lehtonen","year":"1980","journal-title":"J. Embryol. exp. Morph"},{"key":"2023060116563918900_JOCES_77_1_197C6","doi-asserted-by":"publisher","first-page":"719","DOI":"10.1104\/pp.71.4.719","article-title":"Regulation of cell shape in Euglena gracilis. I. Involvement of the biological clock, respiration, photosynthesis and cytoskeleton","volume":"71","author":"Lonergan","year":"1983","journal-title":"Pl. Physiol"},{"key":"2023060116563918900_JOCES_77_1_197C7","doi-asserted-by":"publisher","first-page":"37","DOI":"10.1242\/jcs.71.1.37","article-title":"Regulation of cell shape in Euglena gracilis. II. The effects of altered extra- and intracellular Ca2+ concentrations and the effect of calmodulin antagonists","volume":"71","author":"Lonergan","year":"1984","journal-title":"F. Cell Sci"},{"key":"2023060116563918900_JOCES_77_1_197C8","doi-asserted-by":"publisher","first-page":"99","DOI":"10.1242\/jcs.49.1.99","article-title":"Control of cell shape by calcium in the Euglenophyceae","volume":"49","author":"Murray","year":"1981","journal-title":"F. Cell Sci"},{"key":"2023060116563918900_JOCES_77_1_197C9","doi-asserted-by":"publisher","first-page":"1581","DOI":"10.1021\/bi00536a018","article-title":"Modulation of adenylate cyclase activity by the physical state of pigeon erythrocyte membrane. 1. Parallel drug-induced changes in the bilayer fluidity and adenylate cyclase activity","volume":"21","author":"Salesse","year":"1982","journal-title":"Biochemistry"},{"key":"2023060116563918900_JOCES_77_1_197C10","doi-asserted-by":"publisher","first-page":"227","DOI":"10.1016\/0012-1606(83)90021-0","article-title":"Localization of myosin in the preimplantation mouse embryo","volume":"95","author":"Sobel","year":"1983","journal-title":"Devi Biol"},{"key":"2023060116563918900_JOCES_77_1_197C11","doi-asserted-by":"publisher","first-page":"1083","DOI":"10.1083\/jcb.99.3.1083","article-title":"Distinct cytoskeletal domains revealed in sperm cells","volume":"99","author":"Virtanen","year":"1984","journal-title":"F. Cell Biol"},{"key":"2023060116563918900_JOCES_77_1_197C12","doi-asserted-by":"publisher","first-page":"894","DOI":"10.1083\/jcb.99.3.894","article-title":"Localization of actin and myosin for the study of ameboid movement in Dictyostelium using improved immunofluorescence","volume":"99","author":"Yumura","year":"1984","journal-title":"F. Cell Biol"}],"container-title":["Journal of Cell Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.biologists.com\/jcs\/article-pdf\/77\/1\/197\/2908397\/joces_77_1_197.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/journals.biologists.com\/jcs\/article-pdf\/77\/1\/197\/2908397\/joces_77_1_197.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,6,1]],"date-time":"2023-06-01T17:00:27Z","timestamp":1685638827000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.biologists.com\/jcs\/article\/77\/1\/197\/59589\/Regulation-of-cell-shape-in-Euglena-gracilis-IV"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1985,8,1]]},"references-count":12,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1985,8,1]]}},"URL":"https:\/\/doi.org\/10.1242\/jcs.77.1.197","relation":{},"ISSN":["0021-9533","1477-9137"],"issn-type":[{"value":"0021-9533","type":"print"},{"value":"1477-9137","type":"electronic"}],"subject":[],"published":{"date-parts":[[1985,8,1]]}}}