{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T01:43:22Z","timestamp":1791251002402,"version":"4.1.0"},"reference-count":22,"publisher":"The Company of Biologists","issue":"1","license":[{"start":{"date-parts":[[1987,8,1]],"date-time":"1987-08-01T00:00:00Z","timestamp":554774400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1987,8,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>This report is concerned with the nucleation and organization of microtubule bundles that assemble after \u2018conventional\u2019 centrosomal microtubule-organizing centres have been lost. The microtubule bundles in question span the lengths of wing epidermal cells. Bundles extend between hemidesmosomes at the apical cuticlesecreting surfaces of cells and basal attachment desmosomes that unite the dorsal and ventral epidermal layers of developing wing blades. Furthermore, each bundle includes up to 1500 microtubules and most of the microtubules are composed of 15 protofilaments.<\/jats:p>\n                  <jats:p>Individual cells were serially cross-sectioned at an early stage of bundle assembly. The number of microtubule profiles\/cell cross-section decreased progressively by up to 59% of the most apical values in section sequences cut from fairly apical to more basal levels in the cells. The apical ends of microtubules were associated with numerous small dense plaque-like sites (diameter 0\u00b71\u20130\u00b72 gm), which were specialized regions of plasma membranes at the apical surfaces of cells. Many of the microtubules near apical plaques were not well aligned with each other; they \u2018radiated away\u2019 from cell apices. This was in contrast to the situation at more basal levels where most microtubules were oriented parallel to the longitudinal axes of cells.<\/jats:p>\n                  <jats:p>These findings indicate that the relatively dispersed arrays of apical plasma membrane-associated plaques act as microtubule-nucleating sites to initiate basally directed elongation of bundle microtubules. Apical cell surfaces and their plaques seem to operate as microtubulenucleating and -organizing regions that functionally replace the centrosomal microtubule-organizing centres lost earlier in cell differentiation.<\/jats:p>","DOI":"10.1242\/jcs.88.1.95","type":"journal-article","created":{"date-parts":[[2021,4,26]],"date-time":"2021-04-26T17:16:09Z","timestamp":1619457369000},"page":"95-107","source":"Crossref","is-referenced-by-count":65,"title":["Evidence for microtubule nucleation at plasma membrane-associated sites in\n                    <i>Drosophila<\/i>"],"prefix":"10.1242","volume":"88","author":[{"given":"M. M.","family":"Mogensen","sequence":"first","affiliation":[{"name":"University of St Andrews, St Andrews, Fife KYI6 9TS, UK Department of Zoology and Marme Biology, Bute Buildings ,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. B.","family":"Tucker","sequence":"additional","affiliation":[{"name":"University of St Andrews, St Andrews, Fife KYI6 9TS, UK Department of Zoology and Marme Biology, Bute Buildings ,"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[1987,8,1]]},"reference":[{"key":"2023091602555925100_JOCES_88_1_95C1","first-page":"99","article-title":"The centrosome","volume-title":"In Membrane Structure and Function","author":"Bornens","year":"1984"},{"key":"2023091602555925100_JOCES_88_1_95C2","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1146\/annurev.cb.01.110185.001045","article-title":"Microtubule organizing centers. A","volume":"1","author":"Brinkley","year":"1985","journal-title":"Rev. Cell Biol"},{"key":"2023091602555925100_JOCES_88_1_95C3","doi-asserted-by":"crossref","first-page":"621","DOI":"10.1016\/0092-8674(83)90094-6","article-title":"Centrosome development in early mouse embryo as defined by an autoantibody against pericentriolar meterial","volume":"35","author":"Calarco-Gillam","year":"1983","journal-title":"Cell"},{"key":"2023091602555925100_JOCES_88_1_95C4","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1083\/jcb.101.1.319","article-title":"Microtubule nucleating sites in higher plant cells identified by an auto-antibody against pericentriolar material","volume":"101","author":"Clayton","year":"1985","journal-title":"J. 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General considerations","volume":"43","author":"Fristrom","year":"1975","journal-title":"Devl Biol"},{"key":"2023091602555925100_JOCES_88_1_95C8","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1007\/BF01279808","article-title":"Microtubules and their organizing centres in differentiating guard cells of Adiantum capillus veneris","volume":"115","author":"Galatis","year":"1983","journal-title":"Protoplasma"},{"key":"2023091602555925100_JOCES_88_1_95C9","doi-asserted-by":"crossref","first-page":"601","DOI":"10.1083\/jcb.73.3.601","article-title":"The pericentriolar material in Chinese hamster cells nucleates microtubule formation","volume":"73","author":"Gould","year":"1977","journal-title":"J. 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Sci"},{"key":"2023091602555925100_JOCES_88_1_95C13","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1083\/jcb.98.2.525","article-title":"Tubulin hooks as probes for microtubule polaritv: an analvsis of the method and an evaluation of data on microtubule polarity in the mitotic spindle","volume":"98","author":"McIntosh","year":"1984","journal-title":"J. Cell Biol"},{"key":"2023091602555925100_JOCES_88_1_95C14","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1016\/0012-1606(83)90040-4","article-title":"The morphogenesis of cell hairs on Drosophila wings","volume":"95","author":"Mitchell","year":"1983","journal-title":"Devl Biol"},{"key":"2023091602555925100_JOCES_88_1_95C15","doi-asserted-by":"crossref","first-page":"766","DOI":"10.1083\/jcb.101.3.766","article-title":"Properties of the kinetochore in vitro. II. Microtubule capture and ATP-dependent translocation","volume":"101","author":"Mitchison","year":"1985","journal-title":"J. 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Cell Biol"},{"key":"2023091602555925100_JOCES_88_1_95C20","first-page":"279","article-title":"Centrosomal microtubule-organizing centres and a switch in the control of protofilament number for cell surface-associated microtubules during Drosophila wing morphogenesis","volume":"41","author":"Tucker","year":"1986","journal-title":"Eur. J. Cell Biol"},{"key":"2023091602555925100_JOCES_88_1_95C21","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1007\/BF02982977","article-title":"The genetic control of wing development in Drosophila","volume":"41","author":"Waddington","year":"1941","journal-title":"J. Genet"},{"key":"2023091602555925100_JOCES_88_1_95C22","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1016\/0309-1651(85)90031-1","article-title":"Immunofluorescence microscopy of tubulin and microtubule arrays in plant cells. III. Transition between mitotic\/cytokinetic and interphase microtubule arrays","volume":"9","author":"Wick","year":"1985","journal-title":"Cell Biol. lnt. Rep"}],"container-title":["Journal of Cell Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.biologists.com\/jcs\/article-pdf\/88\/1\/95\/3149824\/joces_88_1_95.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/journals.biologists.com\/jcs\/article-pdf\/88\/1\/95\/3149824\/joces_88_1_95.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,15]],"date-time":"2023-09-15T23:01:20Z","timestamp":1694818880000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.biologists.com\/jcs\/article\/88\/1\/95\/60096\/Evidence-for-microtubule-nucleation-at-plasma"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1987,8,1]]},"references-count":22,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1987,8,1]]}},"URL":"https:\/\/doi.org\/10.1242\/jcs.88.1.95","relation":{},"ISSN":["0021-9533","1477-9137"],"issn-type":[{"value":"0021-9533","type":"print"},{"value":"1477-9137","type":"electronic"}],"subject":[],"published":{"date-parts":[[1987,8,1]]}}}