{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,9,17]],"date-time":"2023-09-17T11:47:50Z","timestamp":1694951270071},"reference-count":53,"publisher":"The Company of Biologists","issue":"2","license":[{"start":{"date-parts":[[1988,2,1]],"date-time":"1988-02-01T00:00:00Z","timestamp":570672000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1988,2,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>TO-2 is a fish cell line derived from the Tilapia ovary. It grows over a wide range of temperature (15\u201334\u00b0C). While most fish cells lack DNA excision repair and are hypersensitive to ultraviolet light (u.v.), Tilapia cells are more u.v.-resistant than mammalian cells. In this paper we report the effects of temperature on DNA replication and u.v. repair in TO-2 cells.<\/jats:p>\n               <jats:p>When the cells were moved from 31 \u00b0C to the sublethal high temperature of 37\u00b0C, the rate of DNA synthesis first decreased to 60%, then speedy recovery soon set in, and after 8h at 37\u00b0C the rate of DNA synthesis overshot the 31 \u00b0C control level by 180%. When moved to low temperature (18\u00b0C) Tilapia cells also showed an initial suppression of DNA synthesis before settling at 30% of the control level. u.v. reduced but could not block DNA synthesis completely. The inhibition was overcome in 3 h at 37, 31 and 25\u00b0C, but not at 18\u00b0C. Initiation of nascent DNA synthesis was blocked at 4 Jm\u22122 in TO-2 cells compared with \u2264ljm\u22122 in mammalian cells. After 9Jm\u22122 u.v. irradiation, low molecular weight DNA replication intermediates started to accumulate, and they could be chased into high molecular weight DNA with little delay.<\/jats:p>\n               <jats:p>TO-2 cells showed low levels of u.v.-induced excision repair; but this was prominent compared with other fish cells. The u.v.-induced incision rate has been measured at various temperatures, and the activation energy of incision estimated to be 13 kcal mol\u22121 (1 cal \u2248 4\u00b7I84 J).<\/jats:p>","DOI":"10.1242\/jcs.89.2.263","type":"journal-article","created":{"date-parts":[[2021,4,26]],"date-time":"2021-04-26T21:15:54Z","timestamp":1619471754000},"page":"263-272","source":"Crossref","is-referenced-by-count":4,"title":["DNA replication and repair of <i>Tilapia<\/i> cells II. Effects of temperature on DNA replication and ultraviolet repair in <i>Tilapia<\/i> ovary cells"],"prefix":"10.1242","volume":"89","author":[{"given":"J. Don","family":"Chen","sequence":"first","affiliation":[{"name":"National Taiwan University Department of Zoology , , Taipei, Taiwan, Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F. H.","family":"Yew","sequence":"additional","affiliation":[{"name":"National Taiwan University Department of Zoology , , Taipei, Taiwan, Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","published-online":{"date-parts":[[1988,2,1]]},"reference":[{"key":"2023091603355322600_JOCES_89_2_263C1","doi-asserted-by":"crossref","first-page":"3830","DOI":"10.1073\/pnas.83.11.3830","article-title":"Survival of UV-irradiated mammalian cells correlates with efficient DNA repair in an essential gene","volume":"83","author":"Bohr","year":"1986","journal-title":"Pnoc. natn. Acad. Sci. 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