{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T17:15:35Z","timestamp":1790874935350,"version":"4.1.0"},"reference-count":26,"publisher":"The Company of Biologists","issue":"4","license":[{"start":{"date-parts":[[1989,4,1]],"date-time":"1989-04-01T00:00:00Z","timestamp":607392000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1989,4,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>Immuno-gold labelling electron microscopy of thin sections was used to determine the distribution of red cell membrane and membrane skeleton proteins in the vicinity of internalized malaria parasites. When examined immediately after invasion (young ring-stage parasites), the parasitophorous vacuole membranes of both Plasmodium falciparum and P. knowlesi were found to be characterized by the essentially complete absence of spectrin, ankyrin and the most abundant transmembrane protein, band 3. P. knowlesi merozoites were trapped in the attached but not internalized state by pretreatment with cytochalasin B. In this merozoitered cell complex antibody labelling showed that band 3 had been eliminated from the region of the host cell membrane in contact with the parasite. Internal vesicles, originating apparently from the site of attachment, were often observed in the red cell. Opposite the attached parasite a cavity was also sometimes seen in the host cell, presumably representing an incipient internal vesicle. The membrane was intact, as judged by the absence of protein (haemoglobin) in the cavity, and, like the membranes surrounding the internal vesicles, was devoid of membrane proteins. A large multilamellar body was sometimes seen in the merozoite close to its point of attachment. The lamellar spacing was about 50 nm. The electron microscope images suggest a diffusion of electron-dense material from the lamellar body into the cavity in the host cell.<\/jats:p>","DOI":"10.1242\/jcs.92.4.691","type":"journal-article","created":{"date-parts":[[2021,4,26]],"date-time":"2021-04-26T21:27:41Z","timestamp":1619472461000},"page":"691-699","source":"Crossref","is-referenced-by-count":50,"title":["Red cell membrane protein distribution during malarial invasion"],"prefix":"10.1242","volume":"92","author":[{"given":"A. R.","family":"Dluzewski","sequence":"first","affiliation":[{"name":"1 Medical Research Council Cell Biophysics Unit, King\u2019s College, 26-29 Drury Loue, London WC2B SRL, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P. R.","family":"Fryer","sequence":"additional","affiliation":[{"name":"2 Clinical Research Centre, WatfordRoad, Hamnv, Middlesex HAI 3UJ, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Griffiths","sequence":"additional","affiliation":[{"name":"2 Clinical Research Centre, WatfordRoad, Hamnv, Middlesex HAI 3UJ, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. J. M.","family":"Wilson","sequence":"additional","affiliation":[{"name":"3 National Institntefor Medical Research, The Ridgetvay. Mill HUI, London NW7 JM, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W. B.","family":"Gratzer","sequence":"additional","affiliation":[{"name":"1 Medical Research Council Cell Biophysics Unit, King\u2019s College, 26-29 Drury Loue, London WC2B SRL, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[1989,4,1]]},"reference":[{"key":"2023071212450807800_JOCES_92_4_691C1","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1083\/jcb.77.1.72","article-title":"Erythrocyte entry by malaria parasites. A moving junction between erythrocyte and parasite","volume":"77","author":"Aikawa","year":"1978","journal-title":"J. Cell Biol"},{"key":"2023071212450807800_JOCES_92_4_691C2","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1083\/jcb.91.1.55","article-title":"Freeze-fracture study on the erythrocyte membrane dunng malanal parasite invasion","volume":"91","author":"Aikawa","year":"1981","journal-title":"J. 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Immunochemical study of the incorporation into the plasma membrane of erythroid cells","volume":"195","author":"Foxwell","year":"1981","journal-title":"Biochem.J"},{"key":"2023071212450807800_JOCES_92_4_691C12","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1016\/0005-2736(76)90260-1","article-title":"Correlation between changes in the membrane organization and susceptibility to phospholipasc C attack induced by ATP depletion of rat erythrocytes","volume":"419","author":"Gazm","year":"1976","journal-title":"Biochim. biophys. Acta"},{"key":"2023071212450807800_JOCES_92_4_691C13","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1016\/0076-6879(82)85046-5","article-title":"Preparation of spectrin","volume":"85","author":"Gratzer","year":"1982","journal-title":"Meth. 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