{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T20:43:59Z","timestamp":1760647439406},"reference-count":24,"publisher":"The Company of Biologists","issue":"4","license":[{"start":{"date-parts":[[1989,8,1]],"date-time":"1989-08-01T00:00:00Z","timestamp":617932800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1989,8,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>Cyclic AMP-dependent protein kinase (cAPK) is the intracellular mediator of signal transduction events involving the adenylate cyclase-cyclic AMP system. A monoclonal antibody (MAb BB1) to the type II regulatory subunit (RII) of cAPK was used in a post-embedding immunogold-labeling procedure to determine the ultrastructural localization of RH in several different secretory cells of the rat. Label was present in nuclei, especially over the heterochromatin, and in the cytoplasm, particularly in areas containing rough endoplasmic reticulum. Immunolabeled RH was also present in secretory granules of the parotid gland, exocrine and endocrine pancreas, seminal vesicle, anterior and intermediate pituitary, and intestinal endocrine cells. Photoaffinity labeling of parotid saliva, pancreatic and seminal fluids with the cyclic AMP analogue, 32P-labeled-8-azido-cyclic AMP, revealed the presence of cyclic AMP-binding proteins with electrophoretic mobilities similar to those of authentic cAPK regulatory subunits. These results confirm our previous observations on the localization of cAPK regulatory subunits in the rat parotid using polyclonal antibodies, and extend them to a number of other exocrine and endocrine cells. The apparent widespread occurrence of cAPK subunits in secretory granules and secretory fluids suggests that cAPK may be involved in specific intragranular regulatory and\/or phosphorylation events, or that it has an unidentified extracellular function.<\/jats:p>","DOI":"10.1242\/jcs.93.4.675","type":"journal-article","created":{"date-parts":[[2021,4,26]],"date-time":"2021-04-26T21:28:38Z","timestamp":1619472518000},"page":"675-681","source":"Crossref","is-referenced-by-count":12,"title":["Regulatory subunits of cyclic AMP-dependent protein kinase: presence in granules and secretion by exocrine and endocrine cells"],"prefix":"10.1242","volume":"93","author":[{"given":"A. R.","family":"Hand","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. I.","family":"Mednieks","sequence":"additional","affiliation":[{"name":"National Institute of Dental Research, National Institutes of Health, Building 10, Room 1A13, Bethesda, Maryland 20892, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","published-online":{"date-parts":[[1989,8,1]]},"reference":[{"key":"2024062903162133800_JOCES_93_4_675C1","first-page":"102","volume-title":"In Cell Biology of the Secretory Process","author":"Bennett","year":"1984"},{"key":"2024062903162133800_JOCES_93_4_675C2","first-page":"19","article-title":"Localization of the regulatory subunit of type II cyclic AMPdependent protein kinase on the cytoplasmic microtubule network of cultured cells","volume":"6","author":"Browne","year":"1982","journal-title":"Cell Biol. bit. Rep"},{"key":"2024062903162133800_JOCES_93_4_675C3","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1083\/jcb.103.1.189","article-title":"Heterogeneous distribution of the cAMP receptor protein RII in the nervous system: evidence for its intracellular accumulation on microtubules, microtubule-organizing centers, and in the area of the Golgi complex","volume":"103","author":"De Camilli","year":"1986","journal-title":"J. Cell Biol"},{"key":"2024062903162133800_JOCES_93_4_675C4","first-page":"133","article-title":"Regulatory mechanisms in the control of protein kinases","volume":"12","author":"Flockhart","year":"1982","journal-title":"CRC Cnt. Rev. Biocheni"},{"key":"2024062903162133800_JOCES_93_4_675C5","doi-asserted-by":"crossref","DOI":"10.1002\/aja.1001850211","article-title":"Localization of cellular regulatory\u2019 proteins using postembedding immunogold labeling","volume-title":"Ain. J. Anat (in press)","author":"Hand","year":"1989"},{"key":"2024062903162133800_JOCES_93_4_675C6","first-page":"491","article-title":"Immunoelectron microscopic localization of catalytic and regulatory subunits of cAMPdependent protein kinases in the parotid gland","volume":"46","author":"Joachim","year":"1988","journal-title":"Eur.J. Cell Biol"},{"key":"2024062903162133800_JOCES_93_4_675C7","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1016\/0006-291X(85)90454-1","article-title":"Regulatory subunit of cAMP-dependent protein kinase inhibits phosphoprotein phosphatase","volume":"130","author":"Khatra","year":"1985","journal-title":"Biochent. biophvs. Res. Contmnn"},{"key":"2024062903162133800_JOCES_93_4_675C8","doi-asserted-by":"crossref","first-page":"965","DOI":"10.1083\/jcb.101.3.965","article-title":"Localization of nuclear subunits of cyclic AMP-dependent protein kinase by the immunocolloidal gold method","volume":"101","author":"Kuettel","year":"1985","journal-title":"J. Cell Biol"},{"key":"2024062903162133800_JOCES_93_4_675C9","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/0006-2952(87)90390-X","article-title":"Protein phosphorylation in rat mast cell granules. Cyclic AMP dependent phosphorylation of a 44K protein associated with broken granules","volume":"36","author":"Kurosawa","year":"1987","journal-title":"Biochent. Phannac"},{"key":"2024062903162133800_JOCES_93_4_675C10","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","article-title":"Cleavage of structural proteins during the assembly of the head of bacteriophage T4","volume":"277","author":"Laemmli","year":"1970","journal-title":"Nature, Land"},{"key":"2024062903162133800_JOCES_93_4_675C11","first-page":"63","article-title":"Regulation of the cellular and subcellular concentrations and distribution of cyclic nucleotide-dependent protein kinases","volume":"18","author":"Lohmann","year":"1984","journal-title":"Adv. Cyclic Nucl. Prot. Phosphorylation Res"},{"key":"2024062903162133800_JOCES_93_4_675C12","doi-asserted-by":"crossref","first-page":"945","DOI":"10.1007\/BF01946451","article-title":"Cyclic AMP-binding proteins in saliva","volume":"40","author":"Mednieks","year":"1984","journal-title":"Expenentia"},{"key":"2024062903162133800_JOCES_93_4_675C13","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1111\/j.1749-6632.1987.tb29503.x","article-title":"Cyclic AMP-dependent protein kinase compartmentalization in stimulated exocrine cells","volume":"494","author":"Mednieks","year":"1987","journal-title":"Ann. N.Y. Acad. Sci"},{"key":"2024062903162133800_JOCES_93_4_675C14","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1177\/37.3.2537353","article-title":"Immunogold localization of the type II regulatory subunit of cyclic AMP-dependent protein kinase. Monoclonal antibody characterization and RII distribution in rat parotid acinar cells","volume":"37","author":"Mednieks","year":"1989","journal-title":"J. Histocheni. Cytochem"},{"key":"2024062903162133800_JOCES_93_4_675C15","first-page":"308","article-title":"Ultrastructural localization of cyclic AMP-dependent protein kinase regulatory subunits in rat parotid acinar cells","volume":"44","author":"Mednieks","year":"1987","journal-title":"Eur. J. Cell Biol"},{"key":"2024062903162133800_JOCES_93_4_675C16","doi-asserted-by":"crossref","first-page":"1039","DOI":"10.1016\/S0092-8674(85)80084-2","article-title":"Cyclic AMP-dependent protein kinase type II is associated with the Golgi complex and with centrosomes","volume":"41","author":"Nigg","year":"1985","journal-title":"Cell"},{"key":"2024062903162133800_JOCES_93_4_675C17","first-page":"242a","article-title":"Protein kinase A subunits localization by immuno electron microscopy during epididymal maturation of ram spermatozoa","volume":"103","author":"Pariset","year":"1986","journal-title":"J. Cell Biol"},{"key":"2024062903162133800_JOCES_93_4_675C18","doi-asserted-by":"crossref","first-page":"7607","DOI":"10.1016\/S0021-9258(19)57440-3","article-title":"Phosphorylation of atrial natriuretic peptides by cyclic AMP-dependent protein kinase","volume":"261","author":"Rittenhouse","year":"1986","journal-title":"J. biol. Cheni"},{"key":"2024062903162133800_JOCES_93_4_675C19","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/0076-6879(83)92005-0","article-title":"Methods of enhancing the frequency of antigen-specific hybridomas","volume":"92","author":"Siraganian","year":"1983","journal-title":"Meth. Enzym"},{"key":"2024062903162133800_JOCES_93_4_675C20","doi-asserted-by":"crossref","first-page":"4350","DOI":"10.1073\/pnas.76.9.4350","article-title":"Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications","volume":"76","author":"Towbin","year":"1979","journal-title":"Proc. natn. Acad. Sci. U.SA"},{"key":"2024062903162133800_JOCES_93_4_675C21","doi-asserted-by":"crossref","first-page":"595","DOI":"10.1016\/0006-291X(87)90315-9","article-title":"A novel proenkephalin processing carboxypeptidase and its activation by cyclic AMP dependent protein kinase","volume":"142","author":"Ljeda","year":"1987","journal-title":"Biochem. biophys. Res. Commun"},{"key":"2024062903162133800_JOCES_93_4_675C22","doi-asserted-by":"crossref","first-page":"568","DOI":"10.1083\/jcb.90.3.568","article-title":"A protein kinase bound to the projection portion of MAP 2 (microtubule-associated protein 2)","volume":"90","author":"Vallee","year":"1981","journal-title":"J. Cell Biol"},{"key":"2024062903162133800_JOCES_93_4_675C23","doi-asserted-by":"crossref","first-page":"1307","DOI":"10.1126\/science.2842866","article-title":"Protein translocation across membranes","volume":"241","author":"Verner","year":"1988","journal-title":"Science"},{"key":"2024062903162133800_JOCES_93_4_675C24","doi-asserted-by":"crossref","first-page":"3297","DOI":"10.1073\/pnas.83.10.3297","article-title":"Exocrine secretion granules contain peptide amidation activity","volume":"83","author":"Von Zastrow","year":"1986","journal-title":"Proc. natn. Acad. Sci. U.SA"}],"container-title":["Journal of Cell Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.biologists.com\/jcs\/article-pdf\/93\/4\/675\/3536045\/joces_93_4_675.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/journals.biologists.com\/jcs\/article-pdf\/93\/4\/675\/3536045\/joces_93_4_675.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,29]],"date-time":"2024-06-29T03:16:55Z","timestamp":1719631015000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.biologists.com\/jcs\/article\/93\/4\/675\/60557\/Regulatory-subunits-of-cyclic-AMP-dependent"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1989,8,1]]},"references-count":24,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1989,8,1]]}},"URL":"https:\/\/doi.org\/10.1242\/jcs.93.4.675","relation":{},"ISSN":["0021-9533","1477-9137"],"issn-type":[{"value":"0021-9533","type":"print"},{"value":"1477-9137","type":"electronic"}],"subject":[],"published":{"date-parts":[[1989,8,1]]}}}