{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,26]],"date-time":"2023-10-26T20:01:57Z","timestamp":1698350517612},"reference-count":41,"publisher":"The Company of Biologists","issue":"3","license":[{"start":{"date-parts":[[1989,11,1]],"date-time":"1989-11-01T00:00:00Z","timestamp":625881600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1989,11,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>An unusual form of red cells (called sequestrocytes) that circulate in the peripheral blood of patients with sickle cell anemia has been identified. In wet-mount light microscopy preparations, sequestro-cytes appear as massively vacuolated erythrocytes. We have shown by transmission electron microscopy and scanning electron microscopy that the morphology of sequestrocytes can be accounted for on the basis of transcellular cross-bonding of the cell membrane. Initially, endocytotic vesicles span the cytoplasmic space, forming putative cytoskeletal fusion zones. Points of fusion of the membrane skeleton bridging the cytoplasmic compartment expand laterally to form linear fusion zones that entrap lakes of hemoglobin within pseudo vacuoles.<\/jats:p>\n               <jats:p>Sequestrocytes can be isolated in the densest layer (1.159 g ml\u22121 or greater) of an arabinogalactan density gradient. These cells can be generated in increased numbers in sickle cell blood by incubating samples in 1.5 mM-acetylphenylhydrazine (APH) solution for two hours at 37\u00b0C. Their formation is partially blocked by incubation with the reducing agent, dithiothreitol (DTT). Our results suggest that these cells represent an expression of oxidative membrane injury in sickle cell anemia.<\/jats:p>","DOI":"10.1242\/jcs.94.3.593","type":"journal-article","created":{"date-parts":[[2021,4,26]],"date-time":"2021-04-26T21:30:29Z","timestamp":1619472629000},"page":"593-600","source":"Crossref","is-referenced-by-count":4,"title":["Sequestrocytes: a manifestation of transcellular cross-bonding of the red cell membrane in sickle cell anemia"],"prefix":"10.1242","volume":"94","author":[{"given":"Ronald S.","family":"Weinstein","sequence":"first","affiliation":[{"name":"Rush Medical College Department of Pathology , , 1653 West Congress Parkway, Chicago, Illinois 60612, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James A.","family":"Warth","sequence":"additional","affiliation":[{"name":"Harper-Grace Hospitals, Wayne State University Department of Medicine , , Detroit, Michigan, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karen","family":"Near","sequence":"additional","affiliation":[{"name":"Harper-Grace Hospitals, Wayne State University Department of Medicine , , Detroit, Michigan, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yehuda","family":"Marikovsky","sequence":"additional","affiliation":[{"name":"The Weizmann Institute of Science Department of Membrane Research , , Rehovot, Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","published-online":{"date-parts":[[1989,11,1]]},"reference":[{"key":"2023071215054571400_JOCES_94_3_593C1","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1159\/000409900","article-title":"Quantitative red cell morphology","volume":"9","author":"Bacus","year":"1984","journal-title":"Monogr. clin. Cytol"},{"key":"2023071215054571400_JOCES_94_3_593C2","doi-asserted-by":"publisher","first-page":"569","DOI":"10.1001\/jama.1968.03140080029007","article-title":"Erythrocyte morphology in patients with sickle cell anemia and pulmonary emboli","volume":"203","author":"Barreras","year":"1968","journal-title":"J. Am. med. Ass"},{"key":"2023071215054571400_JOCES_94_3_593C3","doi-asserted-by":"publisher","first-page":"C208","DOI":"10.1152\/ajpcell.1985.249.3.C208","article-title":"Passive sodium and potassium movements in sickle erythrocytes","volume":"249","author":"Berkowitz","year":"1985","journal-title":"Am. J. Physiol"},{"key":"2023071215054571400_JOCES_94_3_593C4","first-page":"287","article-title":"Preparation of red blood cells (r.b.c) for SEM - A survey of various artifacts","volume-title":"Proc. 5th Ann. Scanning Electron Microscope Symposium and Workshop on Biol. Specimen Preparation for Scanning Electron Microsopy","author":"Bessis","year":"1972"},{"key":"2023071215054571400_JOCES_94_3_593C5","first-page":"191","article-title":"Red cell calcium transport and mechanisms of dehydration in sickle cell anemia","volume-title":"In Approaches to the Theory of Sickle Cell Anemia","author":"Bookchin","year":"1986"},{"key":"2023071215054571400_JOCES_94_3_593C6","first-page":"193","article-title":"Activation of calcium-dependent potassium channels in deoxygenated sickled red cells","volume-title":"In Pathophysiological Aspects of Sickle Cell Vaso-Occlusion","author":"Bookchin","year":"1987"},{"key":"2023071215054571400_JOCES_94_3_593C7","first-page":"633","article-title":"Methaemoglobin Reduction Test: A new simple, in vitro test for identifying primaquine-sensitivity","volume":"22","author":"Brewer","year":"1960","journal-title":"Bull. Wld. Hlth Org"},{"key":"2023071215054571400_JOCES_94_3_593C8","doi-asserted-by":"publisher","first-page":"388","DOI":"10.1126\/science.3961486","article-title":"Regulation of erythrocyte cation and water content in sickle cell anemia","volume":"232","author":"Brugnara","year":"1986","journal-title":"Science"},{"key":"2023071215054571400_JOCES_94_3_593C9","first-page":"25","article-title":"On the thickness of the red cell membrane skeleton: Quantitative electron microscopy of maximally narrowed isthmus regions of intact cells","volume":"12","author":"Bull","year":"1986","journal-title":"Blood Cells"},{"key":"2023071215054571400_JOCES_94_3_593C10","doi-asserted-by":"publisher","first-page":"575","DOI":"10.1111\/j.1365-2141.1982.tb01957.x","article-title":"Erythrocyte hemighosts: a hallmark of severe oxidative injury in vivo","volume":"50","author":"Chan","year":"1982","journal-title":"Br.J. Haem"},{"key":"2023071215054571400_JOCES_94_3_593C11","doi-asserted-by":"publisher","first-page":"422","DOI":"10.1016\/0005-2736(81)90311-4","article-title":"Study of dehydrating effect of the red cell Na+\/K+ pump m nystatin-treated cells with varying Na+ and water contents","volume":"646","author":"Clark","year":"1981","journal-title":"Biochim. biophys. Acta"},{"key":"2023071215054571400_JOCES_94_3_593C12","doi-asserted-by":"publisher","first-page":"1169","DOI":"10.1182\/blood.V51.6.1169.1169","article-title":"Monovalent cation composition and ATP and lipid content of irreversibly sickled cells","volume":"51","author":"Clark","year":"1978","journal-title":"Blood"},{"key":"2023071215054571400_JOCES_94_3_593C13","doi-asserted-by":"publisher","first-page":"500","DOI":"10.1016\/0022-5320(72)90087-1","article-title":"Use of catiomzed ferritin as a label of negative charges on cell surfaces. J\u2019","volume":"38","author":"Danon","year":"1972","journal-title":"Ultrastruct. Res"},{"key":"2023071215054571400_JOCES_94_3_593C14","doi-asserted-by":"publisher","first-page":"87","DOI":"10.1111\/j.1365-2141.1980.tb01186.x","article-title":"Superoxide dismutase, glutathione peroxidase, catalase and lipid peroxidation of normal and sickled erythrocyes","volume":"44","author":"Das","year":"1980","journal-title":"Br.J. Haem"},{"key":"2023071215054571400_JOCES_94_3_593C15","doi-asserted-by":"publisher","first-page":"101","DOI":"10.1016\/0005-2736(76)90433-8","article-title":"Intramembrane particle aggregation in erythrocyte ghosts. IL The influence of spectrin aggregation","volume":"426","author":"Elgseater","year":"1976","journal-title":"Biochim. biophys. Acta"},{"key":"2023071215054571400_JOCES_94_3_593C16","doi-asserted-by":"publisher","first-page":"277","DOI":"10.1016\/0005-2736(86)90430-X","article-title":"Transcellular cross bonding of the red blood cell membrane","volume":"861","author":"Fischer","year":"1986","journal-title":"Biochim. biophys. Acta"},{"key":"2023071215054571400_JOCES_94_3_593C17","doi-asserted-by":"publisher","first-page":"159","DOI":"10.1111\/j.1365-2141.1985.tb02976.x","article-title":"Membrane cross bonding in red cells in favic crisis: A missing link in the mechanism of extravascular haemolysis","volume":"59","author":"Fischer","year":"1985","journal-title":"Br. J. Haemat"},{"key":"2023071215054571400_JOCES_94_3_593C18","doi-asserted-by":"publisher","first-page":"14","DOI":"10.1002\/bies.950080105","article-title":"The membrane skeleton - a distinct structure that regulates the function of cells","volume":"8","author":"Fox","year":"1988","journal-title":"Bioassav"},{"key":"2023071215054571400_JOCES_94_3_593C19","doi-asserted-by":"publisher","first-page":"19","DOI":"10.1042\/bj1730019","article-title":"Mechanism of oxyhaemoglobin breakdown on reaction with acetylphenylhydrazine","volume":"173","author":"French","year":"1978","journal-title":"Biochem. J"},{"key":"2023071215054571400_JOCES_94_3_593C20","doi-asserted-by":"publisher","first-page":"983","DOI":"10.1182\/blood.V51.5.983.983","article-title":"Cation permeability alterations during sickling: Relation to cation composition and cellular hydration of irreversibly sickled cells","volume":"51","author":"Glader","year":"1978","journal-title":"Blood"},{"key":"2023071215054571400_JOCES_94_3_593C21","first-page":"898","article-title":"Physical properties of red cells as related to effects in vivo. IV. Oxidant drugs producing abnormal intracellular concentration of hemoglobin (eccentrocytes) with a rigid-red cell hemolytic svndrome. J","volume":"82","author":"Ham","year":"1973","journal-title":"Lab. din. Med"},{"key":"2023071215054571400_JOCES_94_3_593C22","first-page":"1253","article-title":"Spontaneous oxygen radical generation by sickle erythrocytes","volume":"70","author":"Hebbel","year":"1982","journal-title":"J. din. Invest"},{"key":"2023071215054571400_JOCES_94_3_593C23","first-page":"45A","article-title":"(abstr) Autooxidation and the membrane abnormalities of sickle RBC","volume":"60","author":"Hebbel","year":"1982","journal-title":"Blood"},{"key":"2023071215054571400_JOCES_94_3_593C24","first-page":"1487","article-title":"Cation depletion by the sodium pump in red cells with pathological cation leaks. Sickle cells and xerocytes","volume":"78","author":"Joiner","year":"1986","journal-title":"J. din. Invest"},{"key":"2023071215054571400_JOCES_94_3_593C25","doi-asserted-by":"publisher","first-page":"1727","DOI":"10.1001\/jama.1972.03190390013004","article-title":"\u201cBlister Cells\u201d association with pregnancy, sickle cell disease, and pulmonary infraction","volume":"219","author":"Karayalcin","year":"1972","journal-title":"J. Am. med. Ass"},{"key":"2023071215054571400_JOCES_94_3_593C26","doi-asserted-by":"publisher","first-page":"161","DOI":"10.1007\/BF01870583","article-title":"The band 3-rich membrane of Llama erythrocytes: Studies on cell shape and organization of membrane proteins","volume":"72","author":"Khodadad","year":"1983","journal-title":"J. Membr. Biol"},{"key":"2023071215054571400_JOCES_94_3_593C27","doi-asserted-by":"publisher","first-page":"586","DOI":"10.1038\/315586a0","article-title":"Compartmentalization of sickle-cell calcium in endocytic inside-out vesicles","volume":"315","author":"Lew","year":"1985","journal-title":"Nature, Loud"},{"key":"2023071215054571400_JOCES_94_3_593C28","first-page":"587","article-title":"Red cell filaments: Alterations in physiologic aging","volume-title":"In Contractile Proteins in Muscle and Non-Muscle Cell Systems","author":"Loew","year":"1985"},{"key":"2023071215054571400_JOCES_94_3_593C29","first-page":"265","article-title":"Visualization of the \u201cmembrane skeleton\u201d in human erythrocytes by freeze-etching","volume":"25","author":"Nermut","year":"1981","journal-title":"Eur.J. Cell Biol"},{"key":"2023071215054571400_JOCES_94_3_593C30","doi-asserted-by":"publisher","first-page":"710","DOI":"10.1182\/blood.V67.3.710.710","article-title":"Calcium accumulated by sickle cell anemia cells does not affect their potassium (86R+) flux components","volume":"67","author":"Ortiz","year":"1986","journal-title":"Blood"},{"key":"2023071215054571400_JOCES_94_3_593C31","first-page":"361","article-title":"On the proper use of the Soret ban for hemoglobin detection in erythrocytic cells","volume":"4","author":"Ross","year":"1978","journal-title":"Blood Cells"},{"key":"2023071215054571400_JOCES_94_3_593C32","first-page":"175","article-title":"Membrane skeletal dynamics: Role in modulation of red cell deformability, mobility of transmembrane proteins and shape","volume":"20","author":"Sheetz","year":"1983","journal-title":"Semin. Hemat"},{"key":"2023071215054571400_JOCES_94_3_593C33","first-page":"322","article-title":"Rescherche sur l\u2019absorption des rayons ultraviolets par diverse substances","volume":"61","author":"Soret","year":"1878","journal-title":"Archs. Sa. Phys. Nat"},{"key":"2023071215054571400_JOCES_94_3_593C34","doi-asserted-by":"publisher","first-page":"157","DOI":"10.1016\/0005-2736(88)90547-0","article-title":"Magnitude of calcium influx required to induce dehydration of normal human red cells","volume":"943","author":"Tiffert","year":"1988","journal-title":"Biochim. biophys. Acta"},{"key":"2023071215054571400_JOCES_94_3_593C35","doi-asserted-by":"publisher","first-page":"507","DOI":"10.1182\/blood.V64.2.507.507","article-title":"Density ultracentrifugation of sickle cells during and after pam crisis: increased dense echinocytes in crisis","volume":"64","author":"Warth","year":"1984","journal-title":"Blood"},{"key":"2023071215054571400_JOCES_94_3_593C36","doi-asserted-by":"publisher","first-page":"1777","DOI":"10.1021\/bi00380a041","article-title":"Partial characterization of the copolymerization reaction of erythrocyte membrane band 2 hemichromes","volume":"26","author":"Waugh","year":"1987","journal-title":"Biochemistry"},{"key":"2023071215054571400_JOCES_94_3_593C37","doi-asserted-by":"publisher","first-page":"1155","DOI":"10.1172\/JCI112696","article-title":"Heinz bodies induce clustering of band 3, glycophonn, and ankyrin in sickle cell erythrocytes","volume":"78","author":"Waugh","year":"1986","journal-title":"J. clin. Invest"},{"key":"2023071215054571400_JOCES_94_3_593C38","doi-asserted-by":"publisher","first-page":"545","DOI":"10.1016\/0041-008X(75)90119-2","article-title":"Contribution of Heinz bodies to alterations in red cell deformability","volume":"32","author":"Weinstein","year":"1975","journal-title":"Toxicol, appl. Phann"},{"key":"2023071215054571400_JOCES_94_3_593C39","doi-asserted-by":"publisher","first-page":"325","DOI":"10.1002\/jss.400080310","article-title":"Fine structure of the band 3 protein in human red cell membranes: Freeze-fracture studies","volume":"8","author":"Weinstein","year":"1978","journal-title":"J, supramolec. Struct"},{"key":"2023071215054571400_JOCES_94_3_593C40","first-page":"343","article-title":"Red cell comets: Ultrastructure of axial elongation of the membrane skeleton","volume":"11","author":"Weinstein","year":"1986","journal-title":"Blood Cells"},{"key":"2023071215054571400_JOCES_94_3_593C41","first-page":"589","article-title":"The relationship of glutathione levels and Heinz body formation to irreversibly sickled cells in sickle cell anemia. J","volume":"103","author":"Wetterstroem","year":"1984","journal-title":"Lab. din. Med"}],"container-title":["Journal of Cell Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.biologists.com\/jcs\/article-pdf\/94\/3\/593\/3038755\/joces_94_3_593.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/journals.biologists.com\/jcs\/article-pdf\/94\/3\/593\/3038755\/joces_94_3_593.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,12]],"date-time":"2023-07-12T15:08:29Z","timestamp":1689174509000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.biologists.com\/jcs\/article\/94\/3\/593\/60672\/Sequestrocytes-a-manifestation-of-transcellular"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1989,11,1]]},"references-count":41,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1989,11,1]]}},"URL":"https:\/\/doi.org\/10.1242\/jcs.94.3.593","relation":{},"ISSN":["0021-9533","1477-9137"],"issn-type":[{"value":"0021-9533","type":"print"},{"value":"1477-9137","type":"electronic"}],"subject":[],"published":{"date-parts":[[1989,11,1]]}}}