{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T05:50:58Z","timestamp":1779256258447,"version":"3.51.4"},"reference-count":41,"publisher":"The Company of Biologists","issue":"1","license":[{"start":{"date-parts":[[1991,1,1]],"date-time":"1991-01-01T00:00:00Z","timestamp":662688000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1991,1,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>We have studied the expression of cell polarity in hybrids between two rat thyroid epithelial cells: FRT and FRTL-5. FRT cells are polarized but do not express tissue-specific properties, FRTL-5 are unpolarized and express many thyroid-specific genes. A pool of 170 hybrid clones and five independent clones were characterized. The chromosome complement was that expected from 1:1 fusion of the parental cells. No chromosome loss was observed for several generations. All hybrids were polarized as judged from: (1) morphology, (2) transepithelial resistance, (3) preferential secretion of several proteins either through the apical (e.g. thyroglobulin) or through the basolateral pole, and (4) basolateral trapping of iodide. On the other hand, the expression of thyroid-specific markers: thyroglobulin synthesis and secretion, trapping of iodide, thyrotropin-dependent growth and expression of specific membrane antigens, were greatly reduced or inhibited in the pool and in the isolated clones. We also found that reduction of thyroglobulin synthesis was correlated with the loss of activity of the trans-acting factor TgTF1. We conclude that cell polarity, a property of FRT cells, is dominant in the hybrids whereas thyroid differentiation is recessive.<\/jats:p>","DOI":"10.1242\/jcs.98.1.65","type":"journal-article","created":{"date-parts":[[2021,4,26]],"date-time":"2021-04-26T21:20:15Z","timestamp":1619472015000},"page":"65-73","source":"Crossref","is-referenced-by-count":17,"title":["The polarized epithelial phenotype is dominant in hybrids between polarized and unpolarized rat thyroid cell lines"],"prefix":"10.1242","volume":"98","author":[{"given":"Chiara","family":"Zurzolo","sequence":"first","affiliation":[{"name":"Centro di Endocrinologia ed Oncologia Sperimentale del CNR e Dipartimento di Biologia e Patologia Cellulare e Molecolare, II Facolt\u00e0 di Medicina, Via Pansini 5, 80131 Napoli, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raff Aele","family":"Gentile","sequence":"additional","affiliation":[{"name":"Centro di Endocrinologia ed Oncologia Sperimentale del CNR e Dipartimento di Biologia e Patologia Cellulare e Molecolare, II Facolt\u00e0 di Medicina, Via Pansini 5, 80131 Napoli, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"Mascia","sequence":"additional","affiliation":[{"name":"Centro di Endocrinologia ed Oncologia Sperimentale del CNR e Dipartimento di Biologia e Patologia Cellulare e Molecolare, II Facolt\u00e0 di Medicina, Via Pansini 5, 80131 Napoli, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Corrado","family":"Garbi","sequence":"additional","affiliation":[{"name":"Centro di Endocrinologia ed Oncologia Sperimentale del CNR e Dipartimento di Biologia e Patologia Cellulare e Molecolare, II Facolt\u00e0 di Medicina, Via Pansini 5, 80131 Napoli, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudio","family":"Polistina","sequence":"additional","affiliation":[{"name":"Centro di Endocrinologia ed Oncologia Sperimentale del CNR e Dipartimento di Biologia e Patologia Cellulare e Molecolare, II Facolt\u00e0 di Medicina, Via Pansini 5, 80131 Napoli, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luigi","family":"Aloj","sequence":"additional","affiliation":[{"name":"Centro di Endocrinologia ed Oncologia Sperimentale del CNR e Dipartimento di Biologia e Patologia Cellulare e Molecolare, II Facolt\u00e0 di Medicina, Via Pansini 5, 80131 Napoli, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vittorio Enrico","family":"Awedimento","sequence":"additional","affiliation":[{"name":"Centro di Endocrinologia ed Oncologia Sperimentale del CNR e Dipartimento di Biologia e Patologia Cellulare e Molecolare, II Facolt\u00e0 di Medicina, Via Pansini 5, 80131 Napoli, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lucio","family":"Nitsch","sequence":"additional","affiliation":[{"name":"Centro di Endocrinologia ed Oncologia Sperimentale del CNR e Dipartimento di Biologia e Patologia Cellulare e Molecolare, II Facolt\u00e0 di Medicina, Via Pansini 5, 80131 Napoli, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","published-online":{"date-parts":[[1991,1,1]]},"reference":[{"key":"2024071019004759600_JOCES_98_1_65C1","doi-asserted-by":"publisher","first-page":"163","DOI":"10.1016\/S0074-7696(08)60619-1","article-title":"Thyroid cells in culture","volume":"10","author":"Ambesi-Impiombato","year":"1979","journal-title":"Int. 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