{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T04:43:18Z","timestamp":1767847398050,"version":"3.49.0"},"reference-count":61,"publisher":"The Company of Biologists","issue":"3","license":[{"start":{"date-parts":[[1991,3,1]],"date-time":"1991-03-01T00:00:00Z","timestamp":667785600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1991,3,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>During human fetal development, placental syncytiotrophoblaste actively transport calcium from the maternal to the fetal circulation. Two functional components, a cytosolic Ca2+-binding protein (CaBP) and a Ca2+-ATPase have been identified in the syncytiotrophoblaste of the chorionic villi. We report here the calcium uptake properties of a human choriocarcinoma cell line, JEG-3, which was used as an in vitro model cell system for the syncytiotrophoblaste. In culture, JEG-3 proliferated as large syncytial aggregates expressing typical syncytio-trophoblast markers. 48Ca uptake by JEG-3 was a substrate- and temperature-dependent, membrane-mediated active process that exhibited linear kinetics for up to 7min. Both the CaBP and the Ca2+-ATPase were expressed by JEG-3, on the basis of biochemical, histochemical, immunochemical and\/or mRNA asssays. Immunohistochemistry and in situ hybridization revealed that JEG-3 cells were heterogeneous with respect to the expression of the CaBP. The Ca2+-ATPase activity of JEG-3 was similar to the placental enzyme in terms of sensitivity to specific inhibitors, and was detected histochemically along the cell membrane. Fura-2 Ca2+ imaging revealed that calcium uptake by JEG-3 was not accompanied by a concomitant increase in cytosolic [Ca2+], suggesting a specific Ca2+ sequestration mechanism. The involvement of calciotropic hormonal regulation was evaluated by studying the response of JEG-3 to 1,25-dihydroxy vitamin D3. Calcium uptake was significantly stimulated in a dose-dependent manner by a 24-h treatment of the cells with 1,25-dihydroxy vitamin D3 (optimal dose \u223c0.5 nM); the CaBP level doubled whereas steady-state CaBP mRNA did not, suggesting that CaBP expression was regulated by 1,25-dihydroxy vitamin D3. These observations strongly suggest that the JEG-3 human choriocarcinoma cells should serve as a convenient in vitro model system for studying the cellular mechanism and regulation of transplacental calcium transport.<\/jats:p>","DOI":"10.1242\/jcs.98.3.333","type":"journal-article","created":{"date-parts":[[2021,4,26]],"date-time":"2021-04-26T21:21:13Z","timestamp":1619472073000},"page":"333-342","source":"Crossref","is-referenced-by-count":43,"title":["<i>In vitro<\/i> study of placental trophoblast calcium uptake using JEG-3 human choriocarcinoma cells"],"prefix":"10.1242","volume":"98","author":[{"given":"Rocky S.","family":"Tuan","sequence":"first","affiliation":[{"name":"Thomas Jefferson University, 1015 Walnut Street Department of Orthopaedic Surgery , , Philadelphia, PA 19107, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claire J.","family":"Moore","sequence":"additional","affiliation":[{"name":"Thomas Jefferson University, 1015 Walnut Street Department of Orthopaedic Surgery , , Philadelphia, PA 19107, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jacqueline W.","family":"Brittingham","sequence":"additional","affiliation":[{"name":"Thomas Jefferson University, 1015 Walnut Street Department of Orthopaedic Surgery , , Philadelphia, PA 19107, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James J.","family":"Kirwin","sequence":"additional","affiliation":[{"name":"Thomas Jefferson University, 1015 Walnut Street Department of Orthopaedic Surgery , , Philadelphia, PA 19107, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert E.","family":"Akins","sequence":"additional","affiliation":[{"name":"Thomas Jefferson University, 1015 Walnut Street Department of Orthopaedic Surgery , , Philadelphia, PA 19107, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mayme","family":"Wong","sequence":"additional","affiliation":[{"name":"Thomas Jefferson University, 1015 Walnut Street Department of Orthopaedic Surgery , , Philadelphia, PA 19107, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","published-online":{"date-parts":[[1991,3,1]]},"reference":[{"key":"2023100700415023300_JOCES_98_3_333C1","doi-asserted-by":"publisher","first-page":"169","DOI":"10.1007\/BF01870567","article-title":"Distribution of transport proteins over animal cell membranes","volume":"77","author":"Almers","year":"1984","journal-title":"J. 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Effects of massive doses of ergocalciferol on inorganic phosphate and calcium transfer from maternal plasma to placenta, foetus and placenta after foetectomy. Putative role of 1,25-dihydroxyvitamin D in normal pregnancy","volume":"95","author":"van Bogaert","year":"1987","journal-title":"Archs Ini. Physiol. Biochim"},{"key":"2023100700415023300_JOCES_98_3_333C59","first-page":"155","article-title":"Mechanisms involved in the transfer of calcium across the isolated guinea pig placenta","volume":"5","author":"van Kreel","year":"1983","journal-title":"J. devl Physiol"},{"key":"2023100700415023300_JOCES_98_3_333C60","doi-asserted-by":"publisher","first-page":"375","DOI":"10.1146\/annurev.ph.45.030183.002111","article-title":"Calcium transport proteins, calcium absorption and vitamin D. A","volume":"45","author":"Wasserman","year":"1983","journal-title":"Rev. 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