{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T23:50:01Z","timestamp":1785196201090,"version":"3.55.0"},"reference-count":40,"publisher":"The Company of Biologists","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2003,1,15]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>The relationship between the intracellular ATP concentration[ATP]i and the electrical properties of principal cells was investigated in Malpighian tubules of the yellow fever mosquito, Aedes aegypti. Under control conditions, [ATP]i was 0.91 mmol l-1, the input resistance of the principal cell(Rpc) was 334.1 k\u03a9, and the basolateral membrane was marked by a large K+-conductance and a membrane voltage(Vbl) of -75.8 mV. Peritubular cyanide (CN, 0.3 mmol l-1) reduced [ATP]i to 0.08 mmol l-1 in less than 2 min; however, Vbl dropped to -8 mV and Rpc increased to 3150.8 k\u03a9 in 8 min, while the K+-conductance of the basolateral membrane disappeared. Upon washout of CN, Vbl and Rpc returned to control values within 2 min, and the basolateral membrane recovered its K+-conductance. The recovery of normal [ATP]i took 15 min. Dose-dependence and EC50 values for the CN-inhibition of Vbl and the increase in Rpc were strikingly similar (184.0 \u03bcmol l-1 and 164.4 \u03bcmol l-1). Similar effects of metabolic inhibition were observed with dinitrophenol (DNP), but the EC50 values were 50.3 \u03bcmol l-1 and 71.7 \u03bcmol l-1 for the effects on Vbl and Rpc, respectively. Barium, a blocker of K+-channels, significantly hyperpolarized Vbl to -89.1 mV and increased Rpc to 769.4 k\u03a9 under control conditions, but had no effects during metabolic inhibition. These results illustrate a temporal relationship between[ATP]i and electrogenic and conductive transport pathways in principal cells that is consistent with the role of ATP as an integrator of transport steps at apical and basolateral membranes of the cell. When[ATP]i drops to levels that are 10% of control, the V-type H+-ATPase is inhibited, preventing the extrusion of K+to the tubule lumen. At the same time, basolateral membrane K+-channels close, preventing the entry of K+ from the hemolymph. Intracellular K+ homeostasis is thus protected during metabolic inhibition, allowing the cell to re-establish K+transport when ATP is synthesized again.<\/jats:p>","DOI":"10.1242\/jeb.00066","type":"journal-article","created":{"date-parts":[[2002,12,16]],"date-time":"2002-12-16T21:32:52Z","timestamp":1040074372000},"page":"233-243","source":"Crossref","is-referenced-by-count":27,"title":["The dependence of electrical transport pathways in Malpighian tubules on ATP"],"prefix":"10.1242","volume":"206","author":[{"given":"Daniel S.","family":"Wu","sequence":"first","affiliation":[{"name":"Department of Biomedical Sciences, VRT 8014, Cornell University,Ithaca, NY 14853, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Klaus W.","family":"Beyenbach","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, VRT 8014, Cornell University,Ithaca, NY 14853, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","reference":[{"key":"2021042508484352100_REF1","unstructured":"Aneshansley, D. J., Marler, C. E. and Beyenbach, K. W.(1988). Transepithelial voltage measurements in isolated Malpighian tubules of Aedes aegypti.J. Insect Physiol.35,41-52."},{"key":"2021042508484352100_REF2","doi-asserted-by":"crossref","unstructured":"Anstee, J. H., Baldrick, P. and Bowler, K.(1986). Studies on ouabainbinding to(Na++K+)-ATPase from Malpighian tubules of the locust, Locusta migratoria L. Biochim. Biophys. Acta860, 15-24.","DOI":"10.1016\/0005-2736(86)90493-1"},{"key":"2021042508484352100_REF3","doi-asserted-by":"crossref","unstructured":"Aw, T. Y. and Jones, D. P. (1985). ATP concentration gradients in cytosol of liver cells during hypoxia. Am. J. Physiol.249,C385-392.","DOI":"10.1152\/ajpcell.1985.249.5.C385"},{"key":"2021042508484352100_REF4","doi-asserted-by":"crossref","unstructured":"Azuma, M., Harvey, W. R. and Wieczorek, H.(1995). Stoichiometry of K+\/H+ antiport helps to explain extracellular pH 11 in a model epithelium. FEBS Lett.361,153-156.","DOI":"10.1016\/0014-5793(95)00146-Z"},{"key":"2021042508484352100_REF5","doi-asserted-by":"crossref","unstructured":"Beyenbach, K. W. (1995). Mechanism and regulation of electrolyte transport in Malpighian tubules. J. Insect Physiol.41,197-207.","DOI":"10.1016\/0022-1910(94)00087-W"},{"key":"2021042508484352100_REF6","doi-asserted-by":"crossref","unstructured":"Beyenbach, K. W. (2001). Energizing epithelial transport with the vacuolar H+-ATPase. News Physiol. Sci.16,145-151.","DOI":"10.1152\/physiologyonline.2001.16.4.145"},{"key":"2021042508484352100_REF7","doi-asserted-by":"crossref","unstructured":"Beyenbach, K. W. and Masia, R. (2002). Membrane conductances of principal cells in Malpighian tubules of Aedes aegypti.J. Insect Physiol.48,375-386.","DOI":"10.1016\/S0022-1910(02)00057-4"},{"key":"2021042508484352100_REF8","doi-asserted-by":"crossref","unstructured":"Beyenbach, K. W. and Petzel, D. H. (1987). Diuresis in mosquitoes: Role of a natriuretic factor. News Physiol. Sci.2,171-175.","DOI":"10.1152\/physiologyonline.1987.2.5.171"},{"key":"2021042508484352100_REF9","doi-asserted-by":"crossref","unstructured":"Brauer, D. and Tu, S. I. (1994). Effects of ATP analogs on the proton pumping by the vacuolar H+-ATPase from maize roots. Physiol. Plant.91,442-448.","DOI":"10.1111\/j.1399-3054.1994.tb02972.x"},{"key":"2021042508484352100_REF10","doi-asserted-by":"crossref","unstructured":"Dekker, L. R., Fiolet, J. W., VanBavel, E., Coronel, R., Opthof,T., Spaan, J. A. and Janse, M. J. (1996). Intracellular Ca2+, intracellular electrical coupling, and mechanical activity in ischemic rabbit papillary muscle. Circ. Res.79,237-246.","DOI":"10.1161\/01.RES.79.2.237"},{"key":"2021042508484352100_REF11","doi-asserted-by":"crossref","unstructured":"Feng, Y. and Forgac, M. (1994). Inhibition of vacuolar H+-ATPase by disulfide bond formation between cysteine 254 and cysteine 532 in subunit A. J. Biol. Chem.269,13224-13230.","DOI":"10.1016\/S0021-9258(17)36822-9"},{"key":"2021042508484352100_REF12","doi-asserted-by":"crossref","unstructured":"Grieco, M. A. B. and Lopes, A. G. (1997). 5-hydroxytryptamine regulates the Na+\/K+-ATPase activity in Malpighian tubules of Rhodnius prolixus: Evidence for involvement of G-protein and cAMP-dependent protein kinase. Arch. Insect Biochem. Physiol.36,203-214.","DOI":"10.1002\/(SICI)1520-6327(1997)36:3<203::AID-ARCH4>3.0.CO;2-U"},{"key":"2021042508484352100_REF13","doi-asserted-by":"crossref","unstructured":"Haley, C. A. and O'Donnell, M. J. (1997). K+ reabsorption by the lower Malpighian tubule of Rhodnius prolixus: Inhibition by Ba2+ and blockers of H+\/K+-ATPases. J. Exp. Biol.200,139-147.","DOI":"10.1242\/jeb.200.1.139"},{"key":"2021042508484352100_REF14","doi-asserted-by":"crossref","unstructured":"Harvey, B. J. (1995). Cross-talk between sodium and potassium channels in tight epithelia. Kidney Int.48,1191-1199.","DOI":"10.1038\/ki.1995.402"},{"key":"2021042508484352100_REF15","doi-asserted-by":"crossref","unstructured":"Harvey, W. R. and Wieczorek, H. (1997). Animal plasma membrane energization by chemiosmotic H+ V-ATPases. J. Exp. Biol.200,203-216.","DOI":"10.1242\/jeb.200.2.203"},{"key":"2021042508484352100_REF16","unstructured":"Hille, B. (2001). Ion Channels of Excitable Membranes. Sunderland: Sinauer Associates,Inc."},{"key":"2021042508484352100_REF17","doi-asserted-by":"crossref","unstructured":"Hyde, D., Baldrick, P., Marshall, S. L. and Anstee, J. H.(2001). Rubidium reduces potassium permeability and fluid secretion in Malpighian tubules of Locusta migratoria, L. J. Insect Physiol.47,629-637.","DOI":"10.1016\/S0022-1910(00)00152-9"},{"key":"2021042508484352100_REF18","doi-asserted-by":"crossref","unstructured":"Kane, P. M. and Parra, K. J. (2000). Assembly and regulation of the yeast vacuolar H+-ATPase. J. Exp. Biol.203,81-87.","DOI":"10.1242\/jeb.203.1.81"},{"key":"2021042508484352100_REF19","doi-asserted-by":"crossref","unstructured":"Lee, J. K., John, S. A. and Weiss, J. N.(1999). Novel gating mechanism of polyamine block in the strong inward rectifier K channel Kir2.1. J. Gen. Physiol.113,555-564.","DOI":"10.1085\/jgp.113.4.555"},{"key":"2021042508484352100_REF20","doi-asserted-by":"crossref","unstructured":"Leyssens, A., Dijkstra, S., Van Kerkhove, E. and Steels, P.(1994). Mechanisms of K+ uptake across the basal membrane of Malpighian tubules of Formica polyctena: The effect of ions and inhibitors. J. Exp. Biol.195,123-145.","DOI":"10.1242\/jeb.195.1.123"},{"key":"2021042508484352100_REF21","doi-asserted-by":"crossref","unstructured":"Leyssens, A., Zhang, S.-L., Van Kerkhove, E. and Steels, P.(1993). Both dinitrophenol and Ba2+ reduce KCl and fluid secretion in Malpighian tubules of Formica: The role of the apical H+ and K+ concentration gradient. J. Insect Physiol.39,1061-1073.","DOI":"10.1016\/0022-1910(93)90130-J"},{"key":"2021042508484352100_REF22","doi-asserted-by":"crossref","unstructured":"Linton, S. M. and O'Donnell, M. J. (1999). Contributions of K+:Cl- cotransport and Na+\/K+-ATPase to basolateral ion transport in Malpighian tubules of Drosophila melanogaster.J. Exp. Biol.202,1561-1570.","DOI":"10.1242\/jeb.202.11.1561"},{"key":"2021042508484352100_REF23","doi-asserted-by":"crossref","unstructured":"Masia, R., Aneshansley, D., Nagel, W., Nachman, R. J. and Beyenbach, K. W. (2000). Voltage clamping single cells in intact Malpighian tubules of mosquitoes. Am. J. Physiol.279,F747-F754.","DOI":"10.1152\/ajprenal.2000.279.4.F747"},{"key":"2021042508484352100_REF24","doi-asserted-by":"crossref","unstructured":"McNicholas, C. M., MacGregor, G. G., Islas, L. D., Yang, Y.,Hebert, S. C. and Giebisch, G. (1998). pH-dependent modulation of the cloned renal K+ channel, ROMK. Am. J. Physiol.275,F972-F981.","DOI":"10.1152\/ajprenal.1998.275.6.F972"},{"key":"2021042508484352100_REF25","doi-asserted-by":"crossref","unstructured":"Merzendorfer, H., Graf, R., Huss, M., Harvey, W. R. and Wieczorek, H. (1997). Regulation of proton-translocating V-ATPases. J. Exp. Biol.200,225-235.","DOI":"10.1242\/jeb.200.2.225"},{"key":"2021042508484352100_REF26","unstructured":"Neufeld, D. S. and Leader, J. P. (1998). Electrochemical characteristics of ion secretion in Malpighian tubules of the New Zealand alpine weta (Hemideina maori). J. Insect Physiol.44,39-48."},{"key":"2021042508484352100_REF27","doi-asserted-by":"crossref","unstructured":"Nicolson, S. and Isaacson, L. (1990). Patch clamp of the basal membrane of beetle Malpighian tubules: Direct demonstration of potassium channels. J. Insect Physiol.36,877-884.","DOI":"10.1016\/0022-1910(90)90175-F"},{"key":"2021042508484352100_REF28","doi-asserted-by":"crossref","unstructured":"Onken, H., Zeiske, W. and Harvey, B. (1990). Effect of mucosal H+ and chemical modification on transcellular K+ current in frog skin. Biochim. Biophys. Acta1024,95-102.","DOI":"10.1016\/0005-2736(90)90212-7"},{"key":"2021042508484352100_REF29","doi-asserted-by":"crossref","unstructured":"Pannabecker, T. L., Aneshansley, D. J. and Beyenbach, K. W.(1992). Unique electrophysiological effects of dinitrophenol in Malpighian tubules. Am. J. Physiol.263,R609-R614.","DOI":"10.1152\/ajpregu.1992.263.3.R609"},{"key":"2021042508484352100_REF30","doi-asserted-by":"crossref","unstructured":"Pannabecker, T. L., Hayes, T. K. and Beyenbach, K. W.(1993). Regulation of epithelial shunt conductance by the peptide leucokinin. J. Membr. Biol.132, 63-76.","DOI":"10.1007\/BF00233052"},{"key":"2021042508484352100_REF31","doi-asserted-by":"crossref","unstructured":"Schweikl, H., Klein, U., Schindlbeck, M. and Wieczorek, H.(1989). A vacuolar-type ATPase, partially purified from potassium transporting plasma membranes of tobacco hornworm midgut. J. Biol. Chem.264,11136-11142.","DOI":"10.1016\/S0021-9258(18)60440-5"},{"key":"2021042508484352100_REF32","doi-asserted-by":"crossref","unstructured":"Simon, B. J. and Burckhardt, G. (1990). Characterization of inside-out oriented H+-ATPases in cholate-pretreated renal brush-border membrane vesicles. J. Membr. Biol.117,141-151.","DOI":"10.1007\/BF01868681"},{"key":"2021042508484352100_REF33","doi-asserted-by":"crossref","unstructured":"Weltens, R., Leyssens, A., Zhang, S. L., Lohrmann, E., Steels,P. and Van Kerkhove, E. (1992). Unmasking of the apical electrogenic proton pump in isolated Malpighian tubules (Formica polyctena) by the use of barium. Cell. Physiol. Biochem.2,101-116.","DOI":"10.1159\/000154630"},{"key":"2021042508484352100_REF34","doi-asserted-by":"crossref","unstructured":"Wessing, A., Bertram, G. and Zierold, K.(1993). Effects of bafilomycin A1 and amiloride on the apical potassium and proton gradients in Drosophila Malpighian tubules studied by X-ray microanalysis and microelectrode measurements. J. Comp. Physiol. B163,452-462.","DOI":"10.1007\/BF00346929"},{"key":"2021042508484352100_REF35","doi-asserted-by":"crossref","unstructured":"Williams, J. C. and Beyenbach, K. W. (1983). Differential effects of secretagogues on Na and K secretion in the Malpighian tubules of Aedes aegypti (L.). J. Comp. Physiol.149,511-517.","DOI":"10.1007\/BF00690010"},{"key":"2021042508484352100_REF36","doi-asserted-by":"crossref","unstructured":"Williams, J. C. and Beyenbach, K. W. (1984). Differential effects of secretagogues on the electrophysiology of the Malpighian tubules of the yellow fever mosquito. J. Comp. Physiol. B154,301-309.","DOI":"10.1007\/BF02464411"},{"key":"2021042508484352100_REF37","doi-asserted-by":"crossref","unstructured":"Xu, W. and Marshall, A. T. (1999). X-ray microanalysis of the Malpighian tubules of the black field cricket Teleogryllus oceanicus: The roles of Na K ATPase and the Na K 2Cl cotransporter. J. Insect Physiol.45,885-893.","DOI":"10.1016\/S0022-1910(99)00063-3"},{"key":"2021042508484352100_REF38","doi-asserted-by":"crossref","unstructured":"Yu, M. J. and Beyenbach, K. W. (2001). Leucokinin and the modulation of the shunt pathway in Malpighian tubules. J. Insect Physiol.47,263-276.","DOI":"10.1016\/S0022-1910(00)00084-6"},{"key":"2021042508484352100_REF39","doi-asserted-by":"crossref","unstructured":"Zeiske, W., Meyer, H. and Wieczorek, H. (2002). Insect midgut K+ secretion: Concerted run-down of apical\/basolateral transporters with extra- \/intracellular acidity. J. Exp. Biol.205,463-474.","DOI":"10.1242\/jeb.205.4.463"},{"key":"2021042508484352100_REF40","doi-asserted-by":"crossref","unstructured":"Zeiske, W., Van Driessche, W. and Ziegler, R.(1986). Current-noise analysis of the basolateral route of K+ ions across a K+-secreting insect midgut epithelium(Manduca sexta). Pfluegers Arch.407,657-663.","DOI":"10.1007\/BF00582648"}],"container-title":["Journal of Experimental Biology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/journals.biologists.com\/jeb\/article-pdf\/206\/2\/233\/1246763\/233.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/journals.biologists.com\/jeb\/article-pdf\/206\/2\/233\/1246763\/233.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,8]],"date-time":"2024-01-08T12:07:24Z","timestamp":1704715644000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.biologists.com\/jeb\/article\/206\/2\/233\/13903\/The-dependence-of-electrical-transport-pathways-in"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,1,15]]},"references-count":40,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2003,1,15]]}},"URL":"https:\/\/doi.org\/10.1242\/jeb.00066","relation":{},"ISSN":["1477-9145","0022-0949"],"issn-type":[{"value":"1477-9145","type":"electronic"},{"value":"0022-0949","type":"print"}],"subject":[],"published":{"date-parts":[[2003,1,15]]}}}