{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T13:48:19Z","timestamp":1791035299647,"version":"4.1.0"},"reference-count":50,"publisher":"The Company of Biologists","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2003,2,1]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>Percoll density-gradient separation, combined with peanut lectin agglutinin(PNA) binding and magnetic bead separation, was used to separate dispersed fish gill cells into sub-populations. Functional characterization of each of the sub-populations was performed to determine which displayed acid-activated phenamil- and bafilomycin-sensitive Na+ uptake. Analysis of the mechanism(s) of 22Na+ influx was performed in control and acid-activated (addition of 10 mmoll-1 proprionic acid) cells using a variety of Na+ transport inhibitors (ouabain, phenamil,HOE-694 and bumetanide) and a V-type ATPase inhibitor (bafilomycin). We found that cells migrating to a 1.03-1.05 g ml-1 Percoll interface[pavement cells (PVCs)] possessed the lowest rates of Na+ uptake and that influx was unchanged during either bafilomycin (10 nmoll-1) treatment or internal acidification with addition of proprionic acid (10 mmoll-1). Mitochondria-rich (MR) cells that migrated to the 1.05-1.09 g ml-1 interface of the Percoll gradient demonstrated acidification-activated bafilomycin and phenamil-sensitive Na+ influx. Further separation of the MR fraction into PNA+ and PNA- fractions using magnetic separation demonstrated that only the PNA- cells (\u03b1-MR cells)demonstrated phenamil-and bafilomycin-sensitive acid-activated 22Na+ uptake. We confirm the coupling of a V-type H+-ATPase with phenamil-sensitive Na+ uptake activity and conclude that high-density \u03b1-MR cells function in branchial Na+ uptake in freshwater fish.<\/jats:p>","DOI":"10.1242\/jeb.00109","type":"journal-article","created":{"date-parts":[[2003,1,6]],"date-time":"2003-01-06T23:29:54Z","timestamp":1041895794000},"page":"551-559","source":"Crossref","is-referenced-by-count":81,"title":["Localization and characterization of phenamil-sensitive Na+influx in isolated rainbow trout gill epithelial cells"],"prefix":"10.1242","volume":"206","author":[{"given":"Scott D.","family":"Reid","sequence":"first","affiliation":[{"name":"Dept of Biology, Okanagan University College, Kelowna, British Columbia,VIV 1V7, Canada"},{"name":"Dept of Biological Sciences, University of Alberta, Edmonton, Alberta, T5G 2E9, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G. S.","family":"Hawkings","sequence":"additional","affiliation":[{"name":"Dept of Biological Sciences, University of Alberta, Edmonton, Alberta, T5G 2E9, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Galvez","sequence":"additional","affiliation":[{"name":"Dept of Biological Sciences, University of Alberta, Edmonton, Alberta, T5G 2E9, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G. G.","family":"Goss","sequence":"additional","affiliation":[{"name":"Dept of Biological Sciences, University of Alberta, Edmonton, Alberta, T5G 2E9, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","reference":[{"key":"2021042508551456500_REF1","unstructured":"Alvarez de Canessa, C. M., Fyfe, G. K. and Zhang, P.(2000). Structure and regulation of amiloride-sensitive sodium channels. Annu. Rev. 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