{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,18]],"date-time":"2025-10-18T20:32:04Z","timestamp":1760819524797},"reference-count":39,"publisher":"The Company of Biologists","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2003,3,15]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>Malpighian tubules of the mealworm Tenebrio molitor were isolated for intracellular measurement of basolateral (Vbl) and,indirectly, apical (Vap) membrane potentials. In control Ringer (50 mmol l-1 K+, 140 mmol l-1Na+), Vbl was 24mV, cell negative, and Vap was 48 mV, cell negative with reference to the lumen. Ion substitution experiments involving K+ and Na+indicated that both Vbl and Vap were sensitive to the bathing K+ concentration, with the change in Vap being 60-77% that of Vbl. A 10-fold drop in bath [K+] irreversibly decreased fluid secretion rates from 6.38\u00b10.95 nl min-1 (mean \u00b1 S.E.M.) to 1.48\u00b10.52 nl min-1 (N=8). In the presence of 6 mmol l-1 Ba2+, a blocker of basal K+ channels,fluid secretion rates reversibly decreased and the hyperpolarization of both Vbl and Vap seen in 50 mmol l-1 and 140 mmol l-1 K+ indicated a favourable electrochemical gradient for basal K+ entry. In 5 mmol l-1 K+, Ba2+ induced two different responses: Vbl either hyperpolarized by approximately 10 mV or depolarised by approximately 14mV, according to the electrochemical gradient for K+, which was either inward or outward in low bath[K+]. Rubidium, a `permeant' potassium substitute, caused a hyperpolarization of Vbl, indicating the specificity of K+ channels found in Tenebrio tubule cells. Other possible K+ uptake mechanisms located in the basolateral membrane were investigated. Blocking of the putative electroneutral Na+\/K+\/2Cl- cotransporter by 10 \u03bcmol l-1 bumetanide reversibly decreased fluid secretion rates, with no detectable change in membrane potentials. Ouabain (1 mmol l-1), an Na+\/K+-ATPase inhibitor, irreversibly decreased fluid secretion rates but had no effect on electrical potential differences either in the absence or presence of Ba2+. The results implicate K+ channels, the Na+\/K+\/2Cl-contransporter and the Na+\/K+-ATPase in basal K+ and fluid transport of Tenebrio tubule cells.<\/jats:p>","DOI":"10.1242\/jeb.00200","type":"journal-article","created":{"date-parts":[[2003,2,11]],"date-time":"2003-02-11T23:36:12Z","timestamp":1045006572000},"page":"949-957","source":"Crossref","is-referenced-by-count":19,"title":["K+ transport in Malpighian tubules of<i>Tenebrio molitor<\/i>L.: a study of electrochemical gradients and basal K+uptake mechanisms"],"prefix":"10.1242","volume":"206","author":[{"given":"U. I. M.","family":"Wiehart","sequence":"first","affiliation":[{"name":"Department of Zoology and Entomology, University of Pretoria, Pretoria 0002, South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. W.","family":"Nicolson","sequence":"additional","affiliation":[{"name":"Department of Zoology and Entomology, University of Pretoria, Pretoria 0002, South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"Van Kerkhove","sequence":"additional","affiliation":[{"name":"Laboratory of Physiology, Biomed CMK, Limburgs Universitair Centrum, B3590 Diepenbeek, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","reference":[{"key":"2021042508542209200_REF1","doi-asserted-by":"crossref","unstructured":"Anstee, J. H., Baldrick, P. and Bowler, K.(1986). Studies on ouabain-binding to(Na++K+)-ATPase from Malpighian tubules of the locust, Locusta migratoria L. Biochim. Biophys. Acta860, 15-24.","DOI":"10.1016\/0005-2736(86)90493-1"},{"key":"2021042508542209200_REF2","doi-asserted-by":"crossref","unstructured":"Anstee, J. H. and Bell, D. M. (1975). Relationship of Na+-K+-activated ATPase to fluid production by Malpighian tubules of Locusta migratoria.J. Insect Physiol.21,1779-1784.","DOI":"10.1016\/0022-1910(75)90240-1"},{"key":"2021042508542209200_REF3","doi-asserted-by":"crossref","unstructured":"Anstee, J. H. and Bowler K. (1979). Ouabain-sensitivity of insect epithelial tissues. Comp. Biochem. Physiol. A62,763-769.","DOI":"10.1016\/0300-9629(79)90001-X"},{"key":"2021042508542209200_REF4","doi-asserted-by":"crossref","unstructured":"Baker, P. F. and Willis, J. S. (1970). Potassium ions and the binding of cardiac glycosides to mammalian cells. Nature245,521-523.","DOI":"10.1038\/226521a0"},{"key":"2021042508542209200_REF5","doi-asserted-by":"crossref","unstructured":"Baldrick, P., Hyde, D. and Anstee, J. H.(1988). 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Pflu\u0308gers Arch.426,288-295.","DOI":"10.1007\/BF00374784"}],"container-title":["Journal of Experimental Biology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/journals.biologists.com\/jeb\/article-pdf\/206\/6\/949\/1246547\/949.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/journals.biologists.com\/jeb\/article-pdf\/206\/6\/949\/1246547\/949.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,6,4]],"date-time":"2021-06-04T05:16:26Z","timestamp":1622783786000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.biologists.com\/jeb\/article\/206\/6\/949\/14035\/K-transport-in-Malpighian-tubules-of-Tenebrio"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,3,15]]},"references-count":39,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2003,3,15]]}},"URL":"https:\/\/doi.org\/10.1242\/jeb.00200","relation":{},"ISSN":["1477-9145","0022-0949"],"issn-type":[{"value":"1477-9145","type":"electronic"},{"value":"0022-0949","type":"print"}],"subject":[],"published":{"date-parts":[[2003,3,15]]}}}