{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T11:04:48Z","timestamp":1790161488290,"version":"4.1.0"},"reference-count":61,"publisher":"The Company of Biologists","issue":"7","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2003,4,1]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>Reptiles living in heterogeneous thermal environments are often thought to show behavioural thermoregulation or to become inactive when environmental conditions prevent the achievement of preferred body temperatures. By contrast, thermally homogeneous environments preclude behavioural thermoregulation, and ectotherms inhabiting these environments (particularly fish in which branchial respiration requires body temperature to follow water temperature) modify their biochemical capacities in response to long-term seasonal temperature fluctuations. Reptiles may also be active at seasonally varying body temperatures and could, therefore, gain selective advantages from regulating biochemical capacities. Hence, we tested the hypothesis that a reptile (the American alligator Alligator mississippiensis) that experiences pronounced seasonal fluctuations in body temperature will show seasonal acclimatisation in the activity of its metabolic enzymes. We measured body temperatures of alligators in the wild in winter and summer (N=7 alligators in each season), and we collected muscle samples from wild alligators (N=31 in each season) for analysis of metabolic enzyme activity (lactate dehydrogenase, citrate synthase and cytochrome coxidase). There were significant differences in mean daily body temperatures between winter (15.66\u00b10.43\u00b0C; mean \u00b1 S.E.M.) and summer(29.34\u00b10.21\u00b0C), and daily body temperatures fluctuated significantly more in winter compared with summer. Alligators compensated for lower winter temperatures by increasing enzyme activities, and the activities of cytochrome c oxidase and lactate dehydrogenase were significantly greater in winter compared with summer at all assay temperatures. The activity of citrate synthase was significantly greater in the winter samples at the winter body temperature (15\u00b0C) but not at the summer body temperature(30\u00b0C). The thermal sensitivity (Q10) of mitochondrial enzymes decreased significantly in winter compared with in summer. The activity of mitochondrial enzymes was significantly greater in males than in females, but there were no differences between sexes for lactate dehydrogenase activity. The differences between sexes could be the result of the sex-specific seasonal demands for locomotor performance. Our data indicate that biochemical acclimatisation is important in thermoregulation of reptiles and that it is not sufficient to base conclusions about their thermoregulatory ability entirely on behavioural patterns.<\/jats:p>","DOI":"10.1242\/jeb.00223","type":"journal-article","created":{"date-parts":[[2003,2,25]],"date-time":"2003-02-25T18:51:58Z","timestamp":1046199118000},"page":"1193-1200","source":"Crossref","is-referenced-by-count":115,"title":["Seasonal acclimatisation of muscle metabolic enzymes in a reptile(<i>Alligator mississippiensis<\/i>)"],"prefix":"10.1242","volume":"206","author":[{"given":"Frank","family":"Seebacher","sequence":"first","affiliation":[{"name":"School of Biological Sciences A08, University of Sydney, New South Wales 2006, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helga","family":"Guderley","sequence":"additional","affiliation":[{"name":"De\u0301partment de Biologie, Universite\u0301 Laval, Que\u0301bec, PQ G1K 7P4, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruth M.","family":"Elsey","sequence":"additional","affiliation":[{"name":"Louisiana Department of Wildlife and Fisheries, Rockefeller Wildlife Refuge, 5476 Grand Chenier Highway, Grand Chenier, LA 70643, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"III","given":"Phillip L.","family":"Trosclair","sequence":"additional","affiliation":[{"name":"Louisiana Department of Wildlife and Fisheries, Rockefeller Wildlife Refuge, 5476 Grand Chenier Highway, Grand Chenier, LA 70643, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","reference":[{"key":"2021042509184803500_REF1","doi-asserted-by":"crossref","unstructured":"Andrews, R. M., Me\u0301ndez-de la Cruz, F. R.,Villagra\u0301n-Santa Cruz, M. and Rodriguez-Romero, F.(1999). Field and selected body temperatures of the lizards Sceloporus aeneus and Sceloporus bicanthalis.J. Herpetol.33,93-100.","DOI":"10.2307\/1565547"},{"key":"2021042509184803500_","doi-asserted-by":"crossref","unstructured":"Angilletta, M. J. (2001). Thermal and physiological constraints on energy assimilation in a widespread lizard(Sceloporus undulatus). Ecology82,3044-3056.","DOI":"10.1890\/0012-9658(2001)082[3044:TAPCOE]2.0.CO;2"},{"key":"2021042509184803500_REF3","unstructured":"Avery, R. A. (1982). Field studies of body temperatures and thermoregulation. In Biology of the Reptilia, vol. 12 (ed. C. Gans and F. H. Pough), pp. 93-166. New York: Academic Press."},{"key":"2021042509184803500_REF4","doi-asserted-by":"crossref","unstructured":"Baldwin, J., Seymour, R. S. and Webb, G. J. W.(1995). Scaling of metabolic metabolism during exercise in the estuarine crocodile (Crocodylus porosus). Comp. Biochem. Physiol. A112,285-293.","DOI":"10.1016\/0300-9629(95)00100-X"},{"key":"2021042509184803500_REF5","unstructured":"Bartholomew, G. A. (1982). Physiological control of body temperature. In Biology of the Reptilia, vol. 12 (ed. C. Gans and F. H. Pough), pp. 167-211. New York: Academic Press."},{"key":"2021042509184803500_REF6","doi-asserted-by":"crossref","unstructured":"Bartholomew, G. A. and Tucker, V. A. (1963). Control of changes in body temperature, metabolism, and circulation by the agamid lizard, Amphibolurus barbatus.Physiol. Zool.37,199-218.","DOI":"10.1086\/physzool.36.3.30152307"},{"key":"2021042509184803500_REF7","doi-asserted-by":"crossref","unstructured":"Bejan, A. (1997). Constructal tree network for fluid flow between a finite-size volume and one source or sink. Rev. Ge\u0301n. Therm.36,592-604.","DOI":"10.1016\/S0035-3159(97)89986-2"},{"key":"2021042509184803500_REF8","doi-asserted-by":"crossref","unstructured":"Case, T. J. (1976). Seasonal aspects of thermoregulatory behavior in the Chuckawalla, Sauromalus obesus(Reptilia, Lacertilia, Iguanidae). J. Herpetol.10, 85-95.","DOI":"10.2307\/1562788"},{"key":"2021042509184803500_REF9","doi-asserted-by":"crossref","unstructured":"Christian, K. A. and Tracy, C. R. (1981). The effects of the thermal environment on the ability of hatchling Galapagos land iguanas to avoid predation during dispersal. Oecologia49,218-223.","DOI":"10.1007\/BF00349191"},{"key":"2021042509184803500_REF10","doi-asserted-by":"crossref","unstructured":"Christian, K. A., Tracy, C. R. and Porter, W. P.(1983). Seasonal shifts in body temperature and use of microhabitats by Galapagos land iguanas (Conolophus pallidus). Ecology64,463-468.","DOI":"10.2307\/1939965"},{"key":"2021042509184803500_REF11","doi-asserted-by":"crossref","unstructured":"Christian, K. A. and Weavers, B. W. (1996). Thermoregulation of monitor lizards in Australia: an evaluation of methods in thermal biology. Ecol. Monogr.66,139-157.","DOI":"10.2307\/2963472"},{"key":"2021042509184803500_REF12","doi-asserted-by":"crossref","unstructured":"Costanzo, J. P., Litzgus, J. D., Iverson, J. B. and Lee, R. E.,Jr (2000). Seasonal changes in physiology and development of cold hardiness in the hatchling painted turtle Chrysemys picta.J. Exp. Biol.203,3459-3470.","DOI":"10.1242\/jeb.203.22.3459"},{"key":"2021042509184803500_REF13","unstructured":"Cowles, R. B. and Bogert, C. M. (1944). A preliminary study of the thermal requirements of desert reptiles. Bull. Am. Mus. Nat. Hist.83,261-296."},{"key":"2021042509184803500_REF14","doi-asserted-by":"crossref","unstructured":"Crawford, D. L., Pierce, V. A. and Segal, J. A.(1999). Evolutionary physiology of closely related taxa: analyses of enzyme expression. Am. Zool.39,389-400.","DOI":"10.1093\/icb\/39.2.389"},{"key":"2021042509184803500_REF15","doi-asserted-by":"crossref","unstructured":"Crawford, D. L. and Powers, D. A. (1989). 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A. and Somero, G. N. (1997). Amino acid sequence differences cannot fully explain interspecific variation in thermal sensitivities of gobiid fish A4-lactate dehydrogenases(A4-LDHS). J. Exp. Biol.200,1839-1850.","DOI":"10.1242\/jeb.200.13.1839"},{"key":"2021042509184803500_REF20","doi-asserted-by":"crossref","unstructured":"Garenc, C., Couture, P., Laflamme, M.-A. and Guderley, H.(1999). Metabolic correlates of burst swimming capacity of juvenile and adult threespine stickleback (Gasterosteus aculeatus). J. Comp. Physiol. B169,113-122.","DOI":"10.1007\/s003600050201"},{"key":"2021042509184803500_REF21","doi-asserted-by":"crossref","unstructured":"Goolish, E. M. (1991). Aerobic and anaerobic scaling in fish. Biol. Rev.66, 33-56.","DOI":"10.1111\/j.1469-185X.1991.tb01134.x"},{"key":"2021042509184803500_REF22","doi-asserted-by":"crossref","unstructured":"Grant, B. W. (1990). Trade-offs in activity time and physiological performance for thermoregulating desert lizards, Sceloporus merriami.Ecology71,2323-2333.","DOI":"10.2307\/1938643"},{"key":"2021042509184803500_REF23","doi-asserted-by":"crossref","unstructured":"Grant, B. W. and Dunham, A. E. (1988). Thermally imposed time constraints on the activity of the desert lizards, Sceloporus merriami.Ecology69,167-176.","DOI":"10.2307\/1943171"},{"key":"2021042509184803500_REF24","unstructured":"Grbac, I. and Bauwens, D. (2001). Constraints on temperature regulation in two sympatric Podarcis lizards during autumn. Copeia2001,178-186."},{"key":"2021042509184803500_REF25","doi-asserted-by":"crossref","unstructured":"Grigg, G. C. (1978). Metabolic rate,Q10 and respiratory quotient (RQ) in Crocodylus porosus,and some generalizations about low RQ in reptiles. Physiol. Zool.51,354-360.","DOI":"10.1086\/physzool.51.4.30160960"},{"key":"2021042509184803500_REF26","doi-asserted-by":"crossref","unstructured":"Grigg, G. C., Seebacher, F., Beard, L. A. and Morris, D.(1998). Thermal relations of very large crocodiles, Crocodylus porosus, free-ranging in a naturalistic situation. Proc. R. Soc. Lond. B265,1793-1799.","DOI":"10.1098\/rspb.1998.0504"},{"key":"2021042509184803500_REF27","doi-asserted-by":"crossref","unstructured":"Guderley, H. (1990). Functional significance of metabolic responses to thermal acclimation in fish muscle. Am. J. Physiol.259,R245-R252.","DOI":"10.1152\/ajpregu.1990.259.2.R245"},{"key":"2021042509184803500_REF28","doi-asserted-by":"crossref","unstructured":"Guderley, H. and St Pierre, J. (2002). Going with the flow or life in the fast lane: contrasting mitochondrial responses to thermal change. J. Exp. Biol.205,2237-2249.","DOI":"10.1242\/jeb.205.15.2237"},{"key":"2021042509184803500_REF29","doi-asserted-by":"crossref","unstructured":"Hazel, J. R. (1995). Thermal adaptation in biological membranes: Is homeoviscous adaptation the explanation?Annu. Rev. Physiol.57,19-42.","DOI":"10.1146\/annurev.ph.57.030195.000315"},{"key":"2021042509184803500_REF30","doi-asserted-by":"crossref","unstructured":"Hertz, P. E., Huey, R. B. and Stevenson, R. D.(1993). Evaluating temperature regulation by field active ectotherms: the fallacy of the inappropriate question. Am. Nat.142,796-818.","DOI":"10.1086\/285573"},{"key":"2021042509184803500_REF31","unstructured":"Huey, R. B. (1982). Temperature, physiology,and the ecology of reptiles. In Biology of the Reptilia, vol. 12 (ed. C. Gans and F. H. Pough), pp. 25-91. New York: Academic Press."},{"key":"2021042509184803500_REF32","doi-asserted-by":"crossref","unstructured":"Huey, R. B. and Slatkin, M. (1976). Cost and benefit of lizard thermoregulation. Q. Rev. Biol.51,363-384.","DOI":"10.1086\/409470"},{"key":"2021042509184803500_REF33","doi-asserted-by":"crossref","unstructured":"Johnston, I. A. and Temple, G. K. (2002). Thermal plasticity of skeletal muscle phenotype in ectothermic vertebrates and its significance for locomotory behaviour. J. Exp. Biol.205,2305-2322.","DOI":"10.1242\/jeb.205.15.2305"},{"key":"2021042509184803500_REF34","doi-asserted-by":"crossref","unstructured":"Lin, J.-J. and Somero, G. N. (1995). Thermal adaptation of cytoplasmic malate dehydrogenases of eastern pacific barracuda(Sphyraena spp): the role of differential isoenzyme expression. J. Exp. Biol.198,551-560.","DOI":"10.1242\/jeb.198.2.551"},{"key":"2021042509184803500_REF35","doi-asserted-by":"crossref","unstructured":"Lovegrove, B. G., Heldmaier, G. and Ruf, T.(1991). Perspectives of endothermy revisited: the endothermic temperature range. J. Therm. Biol.15,185-197.","DOI":"10.1016\/0306-4565(91)90025-W"},{"key":"2021042509184803500_REF36","doi-asserted-by":"crossref","unstructured":"Martinez, M., Couture, P. and Guderley, H.(1999). Temporal changes in tissue metabolic capacities of wild Atlantic cod Gadus morhua (L.), from Newfoundland. Fish Physiol. Biochem.20,181-191.","DOI":"10.1023\/A:1007747600759"},{"key":"2021042509184803500_REF37","doi-asserted-by":"crossref","unstructured":"Muth, A. (1977). Body temperatures and associated postures of the zebra-tailed lizard (Callisaurus draconoides). Copeia1977,122-125.","DOI":"10.2307\/1443513"},{"key":"2021042509184803500_REF38","doi-asserted-by":"crossref","unstructured":"Norton, S. F., Eppley Z. and Sidell, B. D.(2000). Allometric scaling of maximal enzyme activities in the axial musculature of striped bass, Morone saxatillis (Walbaum). Physiol. Biochem. Zool.73,819-828.","DOI":"10.1086\/318103"},{"key":"2021042509184803500_REF39","doi-asserted-by":"crossref","unstructured":"Olson, J. M. (1987). The effects of seasonal acclimatization on metabolic-enzyme activities in the heart and pectoral muscle of painted turtles Chrysemys picta marginata.Physiol. Zool.60,149-158.","DOI":"10.1086\/physzool.60.1.30158636"},{"key":"2021042509184803500_REF40","doi-asserted-by":"crossref","unstructured":"Olson, J. M. and Crawford, K. M. (1989). The effect of seasonal acclimatization on the buffering capacity and lactate dehydrogenase activity in tissues of the freshwater turtle Chrysemys picta marginata.J. Exp. Biol.145,471-476.","DOI":"10.1242\/jeb.145.1.471"},{"key":"2021042509184803500_REF41","doi-asserted-by":"crossref","unstructured":"Pierce, V. A. and Crawford, D. L. (1997). Phylogenetic analysis of glocolytic enzyme expression. Science275,256-259.","DOI":"10.1126\/science.276.5310.256"},{"key":"2021042509184803500_REF42","doi-asserted-by":"crossref","unstructured":"Robertson, S. L. and Smith, E. N. (1979). Thermal indications of cutaneous blood flow in the American alligator. Comp. Biochem. Physiol. 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Control of heart rate during thermoregulation in the heliothermic lizard Pogona barbata: importance of cholinergic and adrenergic mechanisms. J. Exp. Biol.204,4361-4366.","DOI":"10.1242\/jeb.204.24.4361"},{"key":"2021042509184803500_REF47","doi-asserted-by":"crossref","unstructured":"Seebacher, F. and Grigg, G. C. (1997). Patterns of body temperature in wild freshwater crocodiles, Crocodylus johnstoni: thermoregulation versus thermoconformity, seasonal acclimatisation, and the effect of social interactions. Copeia1997,549-557.","DOI":"10.2307\/1447558"},{"key":"2021042509184803500_REF48","unstructured":"Seebacher, F. and Grigg, G. C. (2001). Social interactions compromise thermoregulation in crocodiles Crocodylus johnstoni and Crocodylus porosus. In Crocodilian Biology and Evolution (ed. G. C. Grigg, F. Seebacher and C. E. Franklin), pp. 310-316. Chipping Norton: Surrey Beatty and Sons."},{"key":"2021042509184803500_REF49","doi-asserted-by":"crossref","unstructured":"Seebacher, F., Grigg, G. C. and Beard, L. A.(1999). Crocodiles as dinosaurs: behavioural thermoregulation in very large ectotherms leads to high and stable body temperatures. J. Exp. Biol.202,77-86.","DOI":"10.1242\/jeb.202.1.77"},{"key":"2021042509184803500_REF50","doi-asserted-by":"crossref","unstructured":"Segal, J. A. and Crawford, D. L. (1994). LDH-B enzyme expression: the mechanisms of altered gene expression in acclimation and evolutionary adaptation. Am. J. Physiol.36,R1150-R1153.","DOI":"10.1152\/ajpregu.1994.267.4.R1150"},{"key":"2021042509184803500_REF51","doi-asserted-by":"crossref","unstructured":"Somero, G. N. and Childress, J. J. (1980). A violation of the metabolism-size scaling paradigm: activities of glycolytic enzymes in muscle increase in large-size fish. Physiol. 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Relative contribution of quantitative and qualitative changes in mitochondria to metabolic compensation during seasonal acclimatisation of rainbow trout Oncorhynchus mykiss.J. Exp. Biol.201,2961-2970.","DOI":"10.1242\/jeb.201.21.2961"},{"key":"2021042509184803500_REF55","doi-asserted-by":"crossref","unstructured":"Tucker, A. D., McCallum, H. I., Limpus, C. J. and McDonald, K. R. (1998). Sex-biased dispersal in a long-lived polygynous reptile (Crocodylus johnstoni). Behav. Ecol. Sociobiol.44,85-90.","DOI":"10.1007\/s002650050519"},{"key":"2021042509184803500_REF56","unstructured":"Van Damme, R., Bauwens, D. and Verheyen R. F.(1987). Thermoregulatory resposes to environmental seasonality by the lizard Lacerta vivipara.Herpetologica43,405-415."},{"key":"2021042509184803500_REF57","unstructured":"Vliet, K. A. (2001). Courtship behaviour of American alligators Alligator mississippiensis. In Crocodilian Biology and Evolution (ed. G. C. Grigg, F. Seebacher and C. E. Franklin), pp. 383-408. 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