{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T06:58:37Z","timestamp":1780383517746,"version":"3.54.1"},"reference-count":49,"publisher":"The Company of Biologists","issue":"11","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2003,6,1]]},"abstract":"<jats:title>SUMMARY<\/jats:title>\n               <jats:p>Previous studies have demonstrated that the synthesis of juvenile hormone(JH) by the isolated corpora allata (CA) complex in vitro as well as the JH titer in the yellow fever mosquito Aedes aegypti are elevated before feeding and low after a blood meal. In the present study, we used an in vitro radiochemical assay to analyze the effect of farnesoic acid(FA) and Aedes allatotropin (Aedes-AT) on the biosynthesis of JH and methyl farnesoate (MF) by the isolated CA complex of A. aegypti adult female. CA complex from day-0 females (0\u20131 h after emergence) exhibited a low basal juvenile hormone III (JH III) biosynthetic activity and did not respond to either allatotropic or FA stimulation. However, incubation of CA complexes from newly emerged females with Aedes-AT plus FA resulted in very high production of JH III. This is the first report suggesting that allatotropin makes corpora allata in newly emerged females capable for JH biosynthesis. When we studied CA complexes dissected from females 1 day after emergence, the stimulatory action of Aedes-AT was strong and dose-dependent,with maximum stimulation in the range of 10\u20138\u201310\u20139 mol l\u20131,suggesting that Aedes-AT is indeed a true allatotropin (a molecule with allatotropic activity) in A. aegypti. The addition to the culture medium of 40 \u03bcmol l\u20131 FA, a JH precursor, resulted in a 9-fold increase in JH III biosynthesis in 2-, 4- and 6-day-old sugar-fed females. The two major labeled products synthesized by the stimulated CA complex were identified as JH III and MF by RP-HPLC and GC\u2013MS. Treatment of CA complexes with FA, but not Aedes-AT, resulted in an increase in MF. Application of both Aedes-AT and FA to the CA complexes of 2-, 4- and 6-day-old females resulted in the same effects as FA alone. These data suggest that in sugar-fed females, FA and Aedes-AT exert different effects on the terminal steps in JH biosynthesis.<\/jats:p>","DOI":"10.1242\/jeb.00371","type":"journal-article","created":{"date-parts":[[2003,5,1]],"date-time":"2003-05-01T21:08:50Z","timestamp":1051823330000},"page":"1825-1832","source":"Crossref","is-referenced-by-count":68,"title":["Stimulation of JH biosynthesis by the corpora allata of adult female <i>Aedes aegypti in vitro<\/i>: effect of farnesoic acid and <i>Aedes<\/i>allatotropin"],"prefix":"10.1242","volume":"206","author":[{"given":"Yiping","family":"Li","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Molecular Biophysics and Center for Insect Science, University of Arizona, Tucson, AZ 85721-0088, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gopalan C.","family":"Unnithan","sequence":"additional","affiliation":[{"name":"Department of Entomology and Center for Insect Science, University of Arizona, AZ 85721, Tucson, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan A.","family":"Veenstra","sequence":"additional","affiliation":[{"name":"Laboratoire de Neuroendocrinologie des Insectes, Universite\u0301 de Bordeaux I, Laboratoire de Neuroendocrinologie, 33405 Talence Cedex,France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rene\u0301","family":"Feyereisen","sequence":"additional","affiliation":[{"name":"INRA, Centre de Recherches d'Antibes, 06606 Antibes Cedex,France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernando G.","family":"Noriega","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biophysics and Center for Insect Science, University of Arizona, Tucson, AZ 85721-0088, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","reference":[{"key":"2021042508382605800_REF1","unstructured":"Audsley, N., Weaver, R. 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Farnesoic acid and allatotropin stimulation in relation to locust allatal maturation. Mol. Cell. Endocrinol.48, 69-76.","DOI":"10.1016\/0303-7207(86)90167-X"},{"key":"2021042508382605800_REF16","doi-asserted-by":"crossref","unstructured":"Gadot, M., Rafaeli, A. and Applebaum, S. W.(1987). Partial purification and characterization of locust allatotropin I. Arch. Insect Biochem. Physiol.4, 213-223.","DOI":"10.1002\/arch.940040307"},{"key":"2021042508382605800_REF17","doi-asserted-by":"crossref","unstructured":"Gilbert, L. I., Granger, N. A. and Roe, R. M.(2000). The JHs: historical facts and speculations on future research directions. Insect Biochem. Mol. Biol.30,617-644.","DOI":"10.1016\/S0965-1748(00)00034-5"},{"key":"2021042508382605800_REF18","unstructured":"Hodkova, M., Okuda, T. and Wagner, R. (1996). Stimulation of corpora allata by extract from neuroendocrine complex;comparison of reproducing and diapausing Pyrrhocoris apterus(Heteroptera: Pyrrhocoridae). Eur. J. Entomol.93,535-543."},{"key":"2021042508382605800_REF19","doi-asserted-by":"crossref","unstructured":"Kataoka, H., Toschi, A., Li, J. P., Carney, R. L., Schooley, D. A. and\n          Kramer, S. J. (1989). Identification of an allatotropin from adult Manduca sexta.Science243,1481-1483.","DOI":"10.1126\/science.243.4897.1481"},{"key":"2021042508382605800_REF20","doi-asserted-by":"crossref","unstructured":"Koladich, P. M., Cusson, M., Bendena, W. G., Tobe, S. S. and McNeil, J.\n          N. (2002). Cardioacceleratory effects of Manduca sexta allatotropin in the true armyworm moth, Pseudaletia unipuncta.Peptides23,645-651.","DOI":"10.1016\/S0196-9781(01)00658-1"},{"key":"2021042508382605800_REF21","doi-asserted-by":"crossref","unstructured":"Kou, R. and Chen, S.-J. (2000). Allatotropic activity in the suboesophageal ganglia and corpora cardiaca of the adult male Loreyi leafworm, Mythimna loreyi.Arch. Insect Biochem. 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(2000). Interaction between Manduca sextaallatotropin and Manduca sexta allatostatin in the fall armyworm Spodoptera frugiperda.Insect Biochem. Mol. Biol.30,719-727.","DOI":"10.1016\/S0965-1748(00)00043-6"},{"key":"2021042508382605800_REF28","unstructured":"Park, C.-L., Hwang, J.-S, Kang, S.-W. and Lee, B.-H.(2002). Molecular characterization of a cDNA from the silk moth Bombyx mori encoding Manduca sexta allatotropin peptide, Zool.Sci.19,287-292."},{"key":"2021042508382605800_REF29","doi-asserted-by":"crossref","unstructured":"Petri, B., Homberg, U., Loesel, R. and Stengl, M.(2002). Evidence for a role of GABA and Mas-allatotropin in photic entrainment of the circadian clock of the cockroach Leucophaea maderae.J. Exp. Biol.205,1459-1469.","DOI":"10.1242\/jeb.205.10.1459"},{"key":"2021042508382605800_REF30","doi-asserted-by":"crossref","unstructured":"Pratt, G. E., Tobe, S. S., Weaver, R. J. and Finney, J. R.(1975). 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Juvenile hormone bisepoxide biosynthesis in vitro by the ring gland of Drosophila melanogaster: a putative juvenile hormone in the higher Diptera. Proc. Natl. Acad. Sci. USA86,1421-1425.","DOI":"10.1073\/pnas.86.4.1421"},{"key":"2021042508382605800_REF34","doi-asserted-by":"crossref","unstructured":"Rudwall, A. J., Sliwowska, J. and Nassel, D. R.(2000). Allatotropin-like neuropeptide in the cockroach abdominal nervous system: myotropic actions, sexually dimorphic distribution and colocalization with serotonin. J. Comp. Neurol.428,159-173.","DOI":"10.1002\/1096-9861(20001204)428:1<159::AID-CNE11>3.0.CO;2-Y"},{"key":"2021042508382605800_REF35","doi-asserted-by":"crossref","unstructured":"Shapiro, A. B., Wheelock, G. D., Hagedorn, H. H., Baker, F. C.,Tsai, L.\n          W. and Schooley, D. A. (1986). Juvenile hormone and juvenile hormone esterase in adult females of the mosquito Aedes aegypti.J. Insect Physiol.32,867-877.","DOI":"10.1016\/0022-1910(86)90102-2"},{"key":"2021042508382605800_REF36","doi-asserted-by":"crossref","unstructured":"Stay, B. (2000). A review of the role of neurosecretion in the control of JH synthesis: a tribute to Berta Scharrer. Insect Biochem. Mol. Biol.30,653-662.","DOI":"10.1016\/S0965-1748(00)00036-9"},{"key":"2021042508382605800_REF37","doi-asserted-by":"crossref","unstructured":"Taylor, P. A., Bhatt, T. R. and Horodyski, F. M.(1996). Molecular characterization and expression analysis of Manduca sexta allatotropin. Eur. J. Biochem.239,588-596.","DOI":"10.1111\/j.1432-1033.1996.0588u.x"},{"key":"2021042508382605800_REF38","doi-asserted-by":"crossref","unstructured":"Teal, P. E. A. (2002). Effects of allatotropin and allatostatin on in vitro production of juvenile hormones by the corpora allata of virgin females of the moths of Heliothis virescens and Manduca sexta.Peptides23,663-669.","DOI":"10.1016\/S0196-9781(01)00660-X"},{"key":"2021042508382605800_REF39","doi-asserted-by":"crossref","unstructured":"Teal, P. E. A., Proveaux, A. T. and Heath, R. R.(2000). Analysis and quantitation of insect juvenile hormones using chemical ionization ion-trap mass spectrometry. Anal. Biochem.277,206-213.","DOI":"10.1006\/abio.1999.4377"},{"key":"2021042508382605800_REF40","doi-asserted-by":"crossref","unstructured":"Tobe, S. S. and Stay, B. (1985). Structure and regulation of the corpus allatum.Adv. Insect Physiol.18,305-432.","DOI":"10.1016\/S0065-2806(08)60043-0"},{"key":"2021042508382605800_REF41","doi-asserted-by":"crossref","unstructured":"Truesdell, P. F., Koladich, P. M., Kataoka, H., Kojima, K.,Suzuki, A.,\n          McNeil, J. N., Mizoguchi, A., Tobe, S. S. and Bendena, W. G. (2000). Molecular characterization of a cDNA from the true armyworm Pseudaletia unipuncta encoding Manduca sextaallatotropin peptide. Insect Biochem. Mol. Biol.30,691-702.","DOI":"10.1016\/S0965-1748(00)00040-0"},{"key":"2021042508382605800_REF42","doi-asserted-by":"crossref","unstructured":"Tu, M.-P., Kou, R., Wang, Z.-S., Stoffolano, J. G., Jr and Yin,C.-M. (2001). Immunolocalization and possible effect of a moth allatotropin-like substance in a fly, Phormia regina (Diptera:Calliphoridae). J. Insect Physiol.47,233-244.","DOI":"10.1016\/S0022-1910(00)00110-4"},{"key":"2021042508382605800_REF43","doi-asserted-by":"crossref","unstructured":"Tu, M.-P., Kou, R., O'Remus, G., Yin, C.-M. and Stoffolano, J. G., Jr (2002). Allatotropic activity in the brain of female Phormia regina (Diptera: Calliphoridae). J. Insect Physiol.48,733-741.","DOI":"10.1016\/S0022-1910(02)00098-7"},{"key":"2021042508382605800_REF44","doi-asserted-by":"crossref","unstructured":"Unni, B. G., Bhaskaran, G., Dahm, K. H. and Hayes, T. K.(1991). Stimulation of juvenile hormone biosynthesis by analogues of a Manduca sexta allatotropin: in vitro studies. Arch. Insect Biochem. Physiol.17,129-142.","DOI":"10.1002\/arch.940170207"},{"key":"2021042508382605800_REF45","doi-asserted-by":"crossref","unstructured":"Unnithan, G. C., Andersen, J. F., Hisano, T., Kuwano, E. and Feyereisen,\n          R. (1995). Inhibition of juvenile-hormone biosynthesis and methyl farnesoate epoxidase activity by 1,5-disubstituted imidazoles in the cockroach, Diploptera punctata.Pestic. Sci.43,13-19.","DOI":"10.1002\/ps.2780430103"},{"key":"2021042508382605800_REF46","doi-asserted-by":"crossref","unstructured":"Veenstra, J. A., Lehman, H. K. and Davis, N. T.(1994). Allatotropin is a cardioacceleratory peptide in Manduca sexta.J. Exp. Biol.188,347-354.","DOI":"10.1242\/jeb.188.1.347"},{"key":"2021042508382605800_REF47","doi-asserted-by":"crossref","unstructured":"Veenstra, J. A. and Costes, L. (1999). Isolation and identification of a peptide and its cDNA from the mosquito Aedes aegypti related to Manduca sexta allatotropin. Peptides20,1145-1151.","DOI":"10.1016\/S0196-9781(99)00117-5"},{"key":"2021042508382605800_REF48","doi-asserted-by":"crossref","unstructured":"Weaver, R. J., Pratt, G. E., Hamnett, A. F. and Jennings, R. C. (1980). The influence of incubation conditions on the rates of juvenile hormone biosynthesis by corpora allata isolated from adult females of the beetle Tenebrio molitor.Insect Biochem.10,245-254.","DOI":"10.1016\/0020-1790(80)90019-0"},{"key":"2021042508382605800_REF49","doi-asserted-by":"crossref","unstructured":"Wennauer, R. and Hoffmann, K. H. (1988). 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