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In situ immunostaining identified the V-type H+-ATPase at the apical membrane of the mitochondrion-rich brush border of principal cells. The V-type H+-ATPase was not found in stellate cells. Measurements of ATPase activity revealed that bafilomycin-sensitive and NO3--sensitive ATPase activity accounted for 50\u201360% of total ATPase activity in crude extracts of Malpighian tubules. No significant ouabain- or vanadate-sensitive Na+\/K+-ATPase activity was detected. These results support the conclusion reached previously in electrophysiological studies that the mechanisms for transepithelial electrolyte secretion in the AedesMalpighian tubules rely on the V-type H+-ATPase as the principal energizer of epithelial transport. Measures of transepithelial Na+and K+ secretion and estimates of the H+ flux mediated by the V-type H+-ATPase suggest a 1:1 stoichiometry for Na+\/H+ and K+\/H+ exchange transport across the apical membrane.<\/jats:p>","DOI":"10.1242\/jeb.00385","type":"journal-article","created":{"date-parts":[[2003,5,27]],"date-time":"2003-05-27T09:50:19Z","timestamp":1054029019000},"page":"2211-2219","source":"Crossref","is-referenced-by-count":77,"title":["The V-type H+-ATPase in Malpighian tubules of\n                    <i>Aedes aegypti<\/i>\n                    : localization and activity"],"prefix":"10.1242","volume":"206","author":[{"given":"Xing-He","family":"Weng","sequence":"first","affiliation":[{"name":"Department of Biomedical Sciences, Cornell University, Ithaca, NY 14853,USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Markus","family":"Huss","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Osnabru\u0308ck, D-49019 Osnabru\u0308ck, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helmut","family":"Wieczorek","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Osnabru\u0308ck, D-49019 Osnabru\u0308ck, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Klaus W.","family":"Beyenbach","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, Cornell University, Ithaca, NY 14853,USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","reference":[{"key":"2021042508304792200_REF1","doi-asserted-by":"crossref","unstructured":"Aronson, P. 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