{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T23:50:00Z","timestamp":1785196200466,"version":"3.55.0"},"reference-count":47,"publisher":"The Company of Biologists","issue":"10","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2004,4,15]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>The mechanisms of K+ entry from the hemolymph into principal cells of Malpighian tubules were investigated in the yellow fever mosquito, Aedes aegypti. The K+ channel blocker Ba2+ (5 mmol l\u20131) significantly decreased transepithelial (TEP) fluid secretion (Vs) from 0.84 nl min\u20131 to 0.37 nl min\u20131 and decreased the K+ concentration in secreted fluid from 119.0 mmol l\u20131 to 54.3 mmol l\u20131 with no change in the Cl\u2013 concentration. Even though the Na+ concentration increased significantly from 116.8 mmol l\u20131 to 144.6 mmol l\u20131, rates of TEP ion secretion significantly decreased for all three ions. In addition,Ba2+ had the following significant electrophysiological effects: it depolarized the TEP voltage (Vt) from 19.4 mV to 17.2 mV,increased the TEP resistance (Rt) from 6.4 k\u03a9cm to 6.9 k\u03a9cm, hyperpolarized the basolateral membrane voltage of principal cells (Vbl) from \u201375.2 mV to \u201388.2 mV and increased the cell input resistance from 363.7 k\u03a9 to 516.3 k\u03a9. These effects of Ba2+ reflect the block of K+ channels that, apparently, are also permeable to Na+. Bumetanide (100\u03bcmol l\u20131) had no effect on TEP fluid secretion and electrical resistance but significantly decreased TEP K+ secretion,consistent with the inhibition of electroneutral Na+\/K+\/2Cl\u2013 cotransport. TEP Na+ secretion significantly increased because other Na+entry pathways remained active. Bumetanide plus Ba2+ completely inhibited TEP electrolyte and fluid secretion, with fast and slow kinetics reflecting the Ba2+ block of basolateral membrane K+channels and the inhibition of Na+\/K+\/2Cl\u2013 cotransport, respectively. The single and combined effects of Ba2+ and bumetanide suggest that(1) K+ channels and Na+\/K+\/2Cl\u2013 cotransport are the primary mechanisms for bringing K+ into cells, (2) K+ channels mediate a significant Na+ influx, (3) Na+ has as many as four entry pathways and (4) the mechanisms of TEP K+ and Na+ secretion are coupled such that complete block of TEP K+ renders the epithelium unable to secrete Na+.<\/jats:p>","DOI":"10.1242\/jeb.00932","type":"journal-article","created":{"date-parts":[[2004,4,9]],"date-time":"2004-04-09T06:57:33Z","timestamp":1081493853000},"page":"1655-1663","source":"Crossref","is-referenced-by-count":41,"title":["Mechanisms of K+ transport across basolateral membranes of principal cells in Malpighian tubules of the yellow fever mosquito,<i>Aedes aegypti<\/i>"],"prefix":"10.1242","volume":"207","author":[{"given":"Brett N.","family":"Scott","sequence":"first","affiliation":[{"name":"Department of Biomedical Sciences, Cornell University, Ithaca, NY 14853, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming-Jiun","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, Cornell University, Ithaca, NY 14853, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lenora W.","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, Cornell University, Ithaca, NY 14853, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Klaus W.","family":"Beyenbach","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, Cornell University, Ithaca, NY 14853, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","reference":[{"key":"2021042509224400900_REF1","doi-asserted-by":"crossref","unstructured":"Audsley, N., Coast, G. M. and Schooley, D. A.(1993). The effects of Manduca sexta diuretic hormone on fluid transport by the Malpighian tubules and cryptonephric complex of Manduca sexta.J. Exp. Biol.178,231-243.","DOI":"10.1242\/jeb.178.1.231"},{"key":"2021042509224400900_REF2","unstructured":"Baldrick, P., Hyde, D. and Anstee, J. H.(1988). Microelectrode studies on Malpighian tubule cells of Locusta migratoria. Effects of external ions and inhibitors. J. Insect Physiol.34,963-976."},{"key":"2021042509224400900_REF3","doi-asserted-by":"crossref","unstructured":"Beyenbach, K. W. (1995). Mechanism and regulation of electrolyte transport in Malpighian tubules. J. Insect Physiol.41,197-207.","DOI":"10.1016\/0022-1910(94)00087-W"},{"key":"2021042509224400900_REF4","doi-asserted-by":"crossref","unstructured":"Beyenbach, K. W. (2001). Energizing epithelial transport with the vacuolar H+-ATPase. News Physiol. Sci.16,145-151.","DOI":"10.1152\/physiologyonline.2001.16.4.145"},{"key":"2021042509224400900_REF5","doi-asserted-by":"crossref","unstructured":"Beyenbach, K. W. and Masia, R. (2002). Membrane conductances of principal cells in Malpighian tubules of Aedes aegypti.J. Insect Physiol.48,375-386.","DOI":"10.1016\/S0022-1910(02)00057-4"},{"key":"2021042509224400900_REF6","doi-asserted-by":"crossref","unstructured":"Beyenbach, K. W. and Petzel, D. H. (1987). Diuresis in mosquitoes: role of a natriuretic factor. News Physiol. Sci.2,171-175.","DOI":"10.1152\/physiologyonline.1987.2.5.171"},{"key":"2021042509224400900_REF7","doi-asserted-by":"crossref","unstructured":"Brzuszczak, I. M., Zhao, J., Bell, C., Stiel, D., Fielding, I.,Percy, J., Smith, R. and O'Loughlin, E. V. (1996). Cyclic AMP-dependent anion secretion in human small and large intestine. J. Gastroenterol. Hepatol.11,804-810.","DOI":"10.1111\/j.1440-1746.1996.tb00084.x"},{"key":"2021042509224400900_REF8","unstructured":"Gillen, C. M. and Bowles, D. W. (2001). Kinetic characterization of a K-Cl cotransporter from the insect cell line Sf9. FASEB J.15,A412."},{"key":"2021042509224400900_REF9","doi-asserted-by":"crossref","unstructured":"Hegarty, J. L., Zhang, B., Carroll, M. C., Cragoe, E. J. J. and Beyenbach, K. W. (1992). Effects of amiloride on isolated Malpighian tubules of the yellow fever mosquito (Aedes aegypti). J. Insect Physiol.38,329-337.","DOI":"10.1016\/0022-1910(92)90057-K"},{"key":"2021042509224400900_REF10","doi-asserted-by":"crossref","unstructured":"Hegarty, J. L., Zhang, B., Pannabecker, T. L., Petzel, D. H.,Baustian, M. D. and Beyenbach, K. W. (1991). Dibutyryl cAMP activates bumetanide-sensitive electrolyte transport in Malpighian tubules. Am. J. Physiol. Cell Physiol.261,C521-C529.","DOI":"10.1152\/ajpcell.1991.261.3.C521"},{"key":"2021042509224400900_REF11","unstructured":"Helman, S. I. (1972). Determination of electrical resistance of the isolated cortical collecting tubule and its possible anatomical location. Yale J. Biol. Med.45,339-345."},{"key":"2021042509224400900_REF12","doi-asserted-by":"crossref","unstructured":"Hopkin, R., Anstee, J. H. and Bowler, K.(2001). An investigation into the effects of inhibitors of fluid production by Locusta Malpighian tubule type I cells on their secretion and elemental composition. J. Insect Physiol.47,359-367.","DOI":"10.1016\/S0022-1910(00)00131-1"},{"key":"2021042509224400900_REF13","doi-asserted-by":"crossref","unstructured":"Huang, D. Y., Osswald, H. and Vallon, V.(2000). Sodium reabsorption in thick ascending limb of Henle's loop: effect of potassium channel blockade in vivo. Br. J. Pharmacol.130,1255-1262.","DOI":"10.1038\/sj.bjp.0703429"},{"key":"2021042509224400900_REF14","doi-asserted-by":"crossref","unstructured":"Hurst, A. M., Duplain, M. and Lapointe, J. Y.(1992). Basolateral membrane potassium channels in rabbit cortical thick ascending limb. Am. J. Physiol. Cell Physiol.263,F262-F267.","DOI":"10.1152\/ajprenal.1992.263.2.F262"},{"key":"2021042509224400900_REF15","doi-asserted-by":"crossref","unstructured":"Hyde, D., Baldrick, P., Marshall, S. L. and Anstee, J. H.(2001). Rubidium reduces potassium permeability and fluid secretion in Malpighian tubules of Locusta migratoria, L. J. Insect Physiol.47,629-637.","DOI":"10.1016\/S0022-1910(00)00152-9"},{"key":"2021042509224400900_REF16","unstructured":"Ianowski, J. P., Christensen, R. J. and O'Donnell, M. J.(2001). Intracellular ion activities in Malpighian tubule cells of Rhodnius prolixus: evaluation of Na\/K\/2Cl cotransport across the basolateral membrane. J. Exp. Biol.205,1645-1655."},{"key":"2021042509224400900_REF17","doi-asserted-by":"crossref","unstructured":"Labarca, P., Santi, C., Zapata, O., Morales, E., Beltr'an, C.,Li'evano, A. and Darszon, A. (1996). A cAMP regulated K+-selective channel from the sea urchin sperm plasma membrane. Dev. Biol.174,271-280.","DOI":"10.1006\/dbio.1996.0072"},{"key":"2021042509224400900_REF18","doi-asserted-by":"crossref","unstructured":"Leyssens, A., Dijkstra, S., Van Kerkhove, E. and Steels, P.(1994). Mechanisms of K+ uptake across the basal membrane of Malpighian tubules of Formica polyctena: the effect of ions and inhibitors. J. Exp. Biol.195,123-145.","DOI":"10.1242\/jeb.195.1.123"},{"key":"2021042509224400900_REF19","doi-asserted-by":"crossref","unstructured":"Leyssens, A., Zhang, S.-L., Van Kerkhove, E. and Steels, P.(1993). Both dinitrophenol and Ba2+ reduce KCl and fluid secretion in Malpighian tubules of Formica: the role of the apical H+ and K+ concentration gradient. J. Insect Physiol.39,1061-1073.","DOI":"10.1016\/0022-1910(93)90130-J"},{"key":"2021042509224400900_REF20","doi-asserted-by":"crossref","unstructured":"Linton, S. M. and O'Donnell, M. J. (1999). Contributions of K+\/Cl\u2013 cotransport and Na+\/K+-ATPase to basolateral ion transport in Malpighian tubules of Drosophila melanogaster.J. Exp. Biol.202,1561-1570.","DOI":"10.1242\/jeb.202.11.1561"},{"key":"2021042509224400900_REF21","doi-asserted-by":"crossref","unstructured":"Marunaka, Y., Niisato, N., O'Brodovich, H., Post, M. and Tanswell, A. K. (1999). Roles of Ca2+ and protein tyrosine kinase in insulin action on cell volume via Na+ and K+ channels and Na+\/K+\/2Cl\u2013cotransporter in fetal rat alveolar type II pneumocyte. J. Membr. Biol.168,91-101.","DOI":"10.1007\/s002329900500"},{"key":"2021042509224400900_REF22","doi-asserted-by":"crossref","unstructured":"Masia, R., Aneshansley, D., Nagel, W., Nachman, R. J. and Beyenbach, K. W. (2000). Voltage clamping single cells in intact Malpighian tubules of mosquitoes. Am. J. Physiol. Renal Physiol.279,F747-F754.","DOI":"10.1152\/ajprenal.2000.279.4.F747"},{"key":"2021042509224400900_REF23","unstructured":"Neufeld, D. S. and Leader, J. P. (1998). Electrochemical characteristics of ion secretion in Malpighian tubules of the New Zealand alpine weta (Hemideina maori). J. Insect Physiol.44,39-48."},{"key":"2021042509224400900_REF24","doi-asserted-by":"crossref","unstructured":"Nicoloson, S. W. and Isaacson, L. C. (1987). Transepithelial and intracellular potentials in isolated Malpighian tubules of tenebrionid beetle. Am. J. Physiol. Renal Physiol.252,F645-F653.","DOI":"10.1152\/ajprenal.1987.252.4.F645"},{"key":"2021042509224400900_REF25","unstructured":"O'Donnell, M. J. and Maddrell, S. H. P. (1984). Secretion by the Malpighian tubules of Rhodnius prolixus. Electrical events. J. Exp. Biol.110,275-290."},{"key":"2021042509224400900_REF26","unstructured":"O'Neill, W. C. and Steinberg, D. F. (1995). Functional coupling of Na+-K+-2Cl\u2013cotransport and Ca2+-dependent K+ channels in vascular endothelial cells. Am. J. Physiol. Cell Physiol.269,C267-C274."},{"key":"2021042509224400900_REF27","doi-asserted-by":"crossref","unstructured":"Pannabecker, T. L., Hayes, T. K. and Beyenbach, K. W.(1993). Regulation of epithelial shunt conductance by the peptide leucokinin. J. Membr. Biol.132, 63-76.","DOI":"10.1007\/BF00233052"},{"key":"2021042509224400900_REF28","doi-asserted-by":"crossref","unstructured":"Petzel, D. H. (2000). Na\/H exchange in mosquito Malpighian tubules. Am. J. Physiol. Regul. Integr. Comp. Physiol.279,R1996-R2003.","DOI":"10.1152\/ajpregu.2000.279.6.R1996"},{"key":"2021042509224400900_REF29","doi-asserted-by":"crossref","unstructured":"Petzel, D. H., Berg, M. M. and Beyenbach, K. W.(1987). Hormone-controlled cAMP-mediated fluid secretion in yellow-fever mosquito. Am. J. Physiol. Regul. Integr. Comp. Physiol.253,R701-R711.","DOI":"10.1152\/ajpregu.1987.253.5.R701"},{"key":"2021042509224400900_REF30","doi-asserted-by":"crossref","unstructured":"Petzel, D. H., Hagedorn, H. H. and Beyenbach, K. W.(1985). Preliminary isolation of mosquito natriuretic factor. Am. J. Physiol. Regul. Integr. Comp. Physiol.249,R379-R386.","DOI":"10.1152\/ajpregu.1985.249.4.R379"},{"key":"2021042509224400900_REF31","doi-asserted-by":"crossref","unstructured":"Ramsay, J. A. (1953). Active transport of potassium by the Malpighian tubules of insects. J. Exp. Biol.93,358-369.","DOI":"10.1242\/jeb.30.3.358"},{"key":"2021042509224400900_REF32","doi-asserted-by":"crossref","unstructured":"Reagan, J. D. (1995). Molecular cloning of a putative Na+-K+-2Cl\u2013 cotransporter from the Malpighian tubules of the tobacco hornworm, Manduca sexta.Insect Biochem. Molec. Biol.25,875-880.","DOI":"10.1016\/0965-1748(95)00049-2"},{"key":"2021042509224400900_REF33","doi-asserted-by":"crossref","unstructured":"Singh, S. K., Mennone, A., Gigliozzi, A., Fraioli, F. and Boyer,J. L. (2001). Cl\u2013 dependent secretory mechanisms in isolated rat bile duct epithelial units. Am. J. Physiol. Gastrointest. Physiol.281,G438-G446.","DOI":"10.1152\/ajpgi.2001.281.2.G438"},{"key":"2021042509224400900_REF34","doi-asserted-by":"crossref","unstructured":"Suzuki, M., Takahashi, K. and Sakai, O. (1994). Regulation by GTP of a Ca2+-activated K+ channel in the apical membrane of rabbit cortical collecting duct cells. J. Membr. Biol.141,43-50.","DOI":"10.1007\/BF00232872"},{"key":"2021042509224400900_REF35","doi-asserted-by":"crossref","unstructured":"Teulon, J., Ronco, P. M. and Vandewalle, A.(1994). Renal cells transformed with SV40 contain a high-conductance calcium-insensitive potassium channel. Am. J. Physiol. Cell Physiol.267,C940-C945.","DOI":"10.1152\/ajpcell.1994.267.4.C940"},{"key":"2021042509224400900_REF36","doi-asserted-by":"crossref","unstructured":"Walter, S. J., Shirley, D. G., Folkerd, E. J. and Unwin, R. J. (2001). Effects of the potassium channel blocker barium on sodium and potassium transport in the rat loop of Henle in vivo. Exp. Physiol.86,469-474.","DOI":"10.1113\/eph8602210"},{"key":"2021042509224400900_REF37","doi-asserted-by":"crossref","unstructured":"Wang, T. (2003). The effects of the potassium channel opener minoxidil on renal electrolytes transport in the loop of Henle. J. Pharm. Exp. Therap.304,833-840.","DOI":"10.1124\/jpet.102.043380"},{"key":"2021042509224400900_REF38","doi-asserted-by":"crossref","unstructured":"Wessing, A., Bertram, G. and Zierold, K.(1993). Effects of bafilomycin A1 and amiloride on the apical potassium and proton gradients in Drosophila Malpighian tubules studied by X-ray microanalysis and microelectrode measurements. J. Comp. Physiol. B163,452-462.","DOI":"10.1007\/BF00346929"},{"key":"2021042509224400900_REF39","doi-asserted-by":"crossref","unstructured":"Wiehart, U. I., Klein, G., Steels, P., Nicolson, S. W. and Van Kerkhove, E. (2003a). K+ transport in Malpighian tubules of Tenebrio molitor L: is a K(ATP) channel involved?J. Exp. Biol.206,959-965.","DOI":"10.1242\/jeb.00201"},{"key":"2021042509224400900_REF40","doi-asserted-by":"crossref","unstructured":"Wiehart, U. I., Nicolson, S. W. and Van Kerkhove, E.(2003b). K+ transport in Malpighian tubules of Tenebrio molitor L: a study of electrochemical gradients and basal K+ uptake mechanisms. J. Exp. Biol.206,949-957.","DOI":"10.1242\/jeb.00200"},{"key":"2021042509224400900_REF41","doi-asserted-by":"crossref","unstructured":"Williams, J. C. and Beyenbach, K. W. (1983). Differential effects of secretagogues on Na and K secretion in the Malpighian tubules of Aedes aegypti (L.). J. Comp. Physiol.149,511-517.","DOI":"10.1007\/BF00690010"},{"key":"2021042509224400900_REF42","doi-asserted-by":"crossref","unstructured":"Williams, J. C. and Beyenbach, K. W. (1984). Differential effects of secretagogues on the electrophysiology of the Malpighian tubules of the yellow fever mosquito. J. Comp. Physiol. B154,301-309.","DOI":"10.1007\/BF02464411"},{"key":"2021042509224400900_REF43","doi-asserted-by":"crossref","unstructured":"Wu, D. S. and Beyenbach, K. W. (2003). The dependence of electrical transport pathways in Malpighian tubules on ATP. J. Exp. Biol.206,233-243.","DOI":"10.1242\/jeb.00066"},{"key":"2021042509224400900_REF44","doi-asserted-by":"crossref","unstructured":"Xu, W. and Marshall, A. T. (1999a). Effects of inhibitors and specific ion-free salines on segmental fluid secretion by the Malpighian tubules of the black field cricket Teleogryllus oceanicus.J. Insect Physiol.45,835-842.","DOI":"10.1016\/S0022-1910(99)00062-1"},{"key":"2021042509224400900_REF45","doi-asserted-by":"crossref","unstructured":"Xu, W. and Marshall, A. T. (1999b). X-ray microanalysis of the Malpighian tubules of the black field cricket Teleogryllus oceanicus: The roles of Na\/K ATPase and the Na\/K\/2Cl cotransporter. J. Insect Physiol.45,885-893.","DOI":"10.1016\/S0022-1910(99)00063-3"},{"key":"2021042509224400900_REF46","unstructured":"Yu, M. J. and Beyenbach, K. W. (2002). Leucokinin activates Ca2+-dependent signal pathway in principal cells of Aedes aegypti Malpighian tubules. Am. J. Physiol. Renal Physiol.283,F499-F508."},{"key":"2021042509224400900_REF47","unstructured":"Yu, M. J., Schooley, D. A. and Beyenbach, K. W.(2003). Cyclic GMP induces a basolateral membrane chloride conductance in principal cells of Aedes aegypti Malpighian tubules. FASEB J.17,A481."}],"container-title":["Journal of Experimental Biology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/journals.biologists.com\/jeb\/article-pdf\/207\/10\/1655\/1548720\/1655.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/journals.biologists.com\/jeb\/article-pdf\/207\/10\/1655\/1548720\/1655.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,12]],"date-time":"2024-01-12T21:48:52Z","timestamp":1705096132000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.biologists.com\/jeb\/article\/207\/10\/1655\/14701\/Mechanisms-of-K-transport-across-basolateral"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,4,15]]},"references-count":47,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2004,4,15]]}},"URL":"https:\/\/doi.org\/10.1242\/jeb.00932","relation":{},"ISSN":["1477-9145","0022-0949"],"issn-type":[{"value":"1477-9145","type":"electronic"},{"value":"0022-0949","type":"print"}],"subject":[],"published":{"date-parts":[[2004,4,15]]}}}