{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T17:20:47Z","timestamp":1773422447659,"version":"3.50.1"},"reference-count":48,"publisher":"The Company of Biologists","issue":"15","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2004,7,1]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>Mechanisms of ion transport during primary urine formation by the Malpighian tubule of Drosophila melanogaster were analyzed through measurements of fluid secretion rate, transepithelial ion flux, basolateral membrane potential (Vbl) and intracellular activities of K+ (aKi) and Cl\u2013(aCli). Calculation of the electrochemical potentials for both ions permitted assessment of the possible contributions of K+ channels, Na+:K+:2Cl\u2013cotransport, and K+:Cl\u2013 cotransport, to net transepithelial ion secretion across the basolateral membrane. The data show that passive movement of both K+ and Cl\u2013 from cell to bath is favoured across the basolateral membrane, indicating that both ions are actively transported into the cell. Contributions of basolateral K+ channels or K+:Cl\u2013 cotransporters to net transepithelial ion secretion can be ruled out. After prior exposure of tubules to ouabain, subsequent addition of bumetanide reduced fluid secretion rate, K+ flux and Na+ flux, indicating a role for a Na+:K+:2Cl\u2013 cotransporter in fluid secretion. Addition of the K+ channel blocker Ba2+ had no effect on aKi or aCli. Addition of Ba2+ unmasked a basolateral Cl\u2013 conductance and the hyperpolarization of Vbl in response to Ba2+ was Cl\u2013-dependent. A new model for fluid secretion proposes that K+ and Cl\u2013 cross the basolateral membrane through a Na+-driven Na+:K+:2Cl\u2013cotransporter and that most of the Na+ that enters the cells is returned to the bath through the Na+\/K+-ATPase.<\/jats:p>","DOI":"10.1242\/jeb.01058","type":"journal-article","created":{"date-parts":[[2004,6,16]],"date-time":"2004-06-16T22:28:46Z","timestamp":1087424926000},"page":"2599-2609","source":"Crossref","is-referenced-by-count":64,"title":["Basolateral ion transport mechanisms during fluid secretion by<i>Drosophila<\/i>Malpighian tubules: Na+ recycling,Na+:K+:2Cl\u2013 cotransport and Cl\u2013 conductance"],"prefix":"10.1242","volume":"207","author":[{"given":"Juan P.","family":"Ianowski","sequence":"first","affiliation":[{"name":"Department of Biology, McMaster University, 1280 Main Street West,Hamilton, Ontario, Canada, L8S 4K1"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael J.","family":"O'Donnell","sequence":"additional","affiliation":[{"name":"Department of Biology, McMaster University, 1280 Main Street West,Hamilton, Ontario, Canada, L8S 4K1"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","reference":[{"key":"2021042509302264300_REF1","doi-asserted-by":"crossref","unstructured":"Ammann, D. 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