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Measurements with this technique revealed secretion of salicylate across the main and distal segments of the Malpighian tubule as well as the midgut, ileum and rectum. The second method used a salicylate-selective microelectrode to measure the concentration of salicylate in fluid droplets secreted by isolated DrosophilaMalpighian tubules set up in a Ramsay secretion assay. Transepithelial salicylate flux was calculated as the product of fluid secretion rate and secreted fluid salicylate concentration. Measurements with this method revealed that salicylate transport was active and saturable; the kinetic parameters Jmax and Kt were 2.72 pmol min-1 tubule-1 and 0.046 mmol l-1,respectively. Measurements of transepithelial salicylate flux determined by both microelectrode methods were in good agreement. Transepithelial flux measurements measured by microelectrodes were also validated by comparing them with measurements of radiolabelled salicylate levels in secreted droplets. Salicylate concentrations in haemolymph samples were measured with salicylate-selective microelectrodes after injection of salicylate into the haemocoel or after insects were fed salicylate-rich diets. The rate of salicylate secretion by Malpighian tubules in vitro was sufficient to account for the measured rate of decline of salicylate concentration in the haemolymph in vivo.<\/jats:p>","DOI":"10.1242\/jeb.01374","type":"journal-article","created":{"date-parts":[[2004,12,15]],"date-time":"2004-12-15T19:14:09Z","timestamp":1103138049000},"page":"93-104","source":"Crossref","is-referenced-by-count":25,"title":["Ion-selective microelectrode analysis of salicylate transport by the Malpighian tubules and gut of<i>Drosophila melanogaster<\/i>"],"prefix":"10.1242","volume":"208","author":[{"given":"Michael J.","family":"O'Donnell","sequence":"first","affiliation":[{"name":"Department of Biology, McMaster University, 1280 Main Street West,Hamilton, Ontario, Canada L8S 4K1"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark R.","family":"Rheault","sequence":"additional","affiliation":[{"name":"Department of Biology, McMaster University, 1280 Main Street West,Hamilton, Ontario, Canada L8S 4K1"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","reference":[{"key":"2021042509503824000_","doi-asserted-by":"crossref","unstructured":"Amman, D. 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