{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T06:49:29Z","timestamp":1791010169975,"version":"4.1.0"},"reference-count":33,"publisher":"The Company of Biologists","issue":"8","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2008,4,15]]},"abstract":"<jats:title>SUMMARY<\/jats:title>\n               <jats:p>We performed experiments on male Australian painted dragon lizards(Ctenophorus pictus) to test the hypothesis that carotenoids can scavenge reactive oxygen species (ROS), protecting the organism from oxidative stress, and that this capacity is reflected in skin colours involved in signalling. Subsequent to 4 weeks of carotenoid treatment we used flow cytometry to analyse unspecified ROS (H2O2, singlet oxygen, superoxide and peroxynitrite level), hereafter termed ROS, and baseline superoxide specifically (bSO in peripheral blood cells). Mean background levels of ROS and bSO did not differ between carotenoid-treated and control males. bSO, which represents the superoxide level in un-manipulated blood, was negatively correlated with colour development in all males,regardless of carotenoid treatment. Thus, carotenoid intake does not reduce circulating levels of ROS or bSO, suggesting that carotenoids are inefficient antioxidants in vivo and, therefore, are unlikely to provide a direct link between oxidative stress and colouration.<\/jats:p>","DOI":"10.1242\/jeb.015065","type":"journal-article","created":{"date-parts":[[2008,3,29]],"date-time":"2008-03-29T07:04:57Z","timestamp":1206774297000},"page":"1257-1261","source":"Crossref","is-referenced-by-count":59,"title":["Carotenoid intake does not mediate a relationship between reactive oxygen species and bright colouration: experimental test in a lizard"],"prefix":"10.1242","volume":"211","author":[{"given":"Mats","family":"Olsson","sequence":"first","affiliation":[{"name":"School of Biological Sciences, The University of Wollongong, NSW 2522,Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark","family":"Wilson","sequence":"additional","affiliation":[{"name":"School of Biological Sciences, The University of Wollongong, NSW 2522,Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caroline","family":"Isaksson","sequence":"additional","affiliation":[{"name":"Department of Zoology, University of Gothenburg, Box 405, SE 413 90 Gothenburg, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tobias","family":"Uller","sequence":"additional","affiliation":[{"name":"School of Biological Sciences, The University of Wollongong, NSW 2522,Australia"},{"name":"Edward Grey Institute, Department of Zoology, University of Oxford, Oxford OX1 3PS, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beth","family":"Mott","sequence":"additional","affiliation":[{"name":"School of Biological Sciences, The University of Wollongong, NSW 2522,Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","reference":[{"key":"2021042511502216100_REF1","doi-asserted-by":"crossref","unstructured":"Alexandrova, M. L. and Bochev, P. G. (2005). Oxidative stress during the chronic phase after stroke. Free. Radic. Biol. Med.39,297-316.","DOI":"10.1016\/j.freeradbiomed.2005.04.017"},{"key":"2021042511502216100_REF2","unstructured":"Alonso-Alvarez, C., Bertrand, S., Devevey, G., Gaillard, M.,Prost, J., Faivre, B. and Sorci, G. (2004). Carotenoids,immunocompetence, and the information content of sexual colors: an experimental test. Am. Nat.164,651-659."},{"key":"2021042511502216100_REF3","unstructured":"Andersson, S. A. and Prager, M. (2006). Bird Coloration, Vol. I: Mechanisms and Measurements(ed. G. E. Hill and K. J. McGraw). Cambridge, MA: Harvard University Press."},{"key":"2021042511502216100_REF4","doi-asserted-by":"crossref","unstructured":"Bertrand, S., Criscuolo, F., Faivre, B. and Sorci, G.(2006). Immune activation increases susceptibility to oxidative tissue damage in Zebra Finches. Funct. Ecol.20,1022-1027.","DOI":"10.1111\/j.1365-2435.2006.01191.x"},{"key":"2021042511502216100_REF5","doi-asserted-by":"crossref","unstructured":"Blount, J. D. (2004). Carotenoids and life-history evolution in animals. Arch. Biochem. Biophys.430,10-15.","DOI":"10.1016\/j.abb.2004.03.039"},{"key":"2021042511502216100_REF6","doi-asserted-by":"crossref","unstructured":"Blount, J. D. and Matheson, S. M. (2006). Effects of carotenoid supply on escape flight responses in zebra finches, Taeniophygis guttata.Anim. Behav.72,595-601.","DOI":"10.1016\/j.anbehav.2005.11.014"},{"key":"2021042511502216100_REF7","doi-asserted-by":"crossref","unstructured":"Blount, J. D., Metcalfe, N. B., Birkhead, T. R. and Surai, P. F. (2003). Carotenoid modulation of immune function and sexual attractiveness in zebra finches. Science300,125-127.","DOI":"10.1126\/science.1082142"},{"key":"2021042511502216100_REF8","unstructured":"Cogger, H. (2000). Reptiles and Amphibians of Australia. Sydney: Reed New Holland."},{"key":"2021042511502216100_","doi-asserted-by":"crossref","unstructured":"Constantini, D., Fanfani, A. and Dell'Omo, G.(2007). Carotenoid availability does not limit the capability of nestling kestrels (Falco tinninculus) to cope with oxidative stress. J. Exp. Biol.210,1238-1244.","DOI":"10.1242\/jeb.002741"},{"key":"2021042511502216100_REF10","doi-asserted-by":"crossref","unstructured":"Czeczuga, B. (1980). Carotenoids in some parts of certain species of lizards. Comp. Biochem. Physiol.55B,755-757.","DOI":"10.1016\/0305-0491(80)90194-7"},{"key":"2021042511502216100_REF11","unstructured":"Hartley, R. C. and Kennedy, M. W. (2004). Are carotenoids a red herring in sexual displays? Trends Ecol. Evol.19,353-354."},{"key":"2021042511502216100_REF12","doi-asserted-by":"crossref","unstructured":"Healey, M., Uller, T. and Olsson, M. (2007). Seeing red: morph-specific contest success, and survival rates, in a color-polymorphic agamid lizard. Anim. Behav.74,337-341.","DOI":"10.1016\/j.anbehav.2006.09.017"},{"key":"2021042511502216100_REF13","doi-asserted-by":"crossref","unstructured":"Ho\u0303rak, P., Zilmer, M., Saks, L., Ots, I., Karu, U. and Zilmer, K. (2006). Antioxidant protection, carotenoids and the costs of immune challenge in greenfinches. J. Exp. Biol.209,4329-4338.","DOI":"10.1242\/jeb.02502"},{"key":"2021042511502216100_REF14","doi-asserted-by":"crossref","unstructured":"Hughes, D. A. (2001). Dietary carotenoids and human immune function. Nutrition17,823-827.","DOI":"10.1016\/S0899-9007(01)00638-4"},{"key":"2021042511502216100_REF15","doi-asserted-by":"crossref","unstructured":"Isaksson, C., McLaughlin, P., Monaghan, P. and Andersson, S.(2007). Carotenoid pigmentation does not reflect total non-enzymatic antioxidant activity in plasma of adult and nestling great tits, Parus major.Funct. 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The chemistry of reactive oxygen species and related free radicals. In Free Radical in Exercise and Aging (ed. Z. Rada'k), pp.1-34. Champaign: Human Kinetics."},{"key":"2021042511502216100_REF20","doi-asserted-by":"crossref","unstructured":"Mayne, S. M. (1996). Beta-carotene,carotenoids, and disease prevention in humans. FASEB J.10,690.","DOI":"10.1096\/fasebj.10.7.8635686"},{"key":"2021042511502216100_REF21","doi-asserted-by":"crossref","unstructured":"McGraw, K. J. (2005). The antioxidant function of many animal pigments: are there consistent health benefits of sexually selected colorants? Anim. Behav.69,757-764.","DOI":"10.1016\/j.anbehav.2004.06.022"},{"key":"2021042511502216100_REF22","doi-asserted-by":"crossref","unstructured":"McGraw, K. J. and Arida, D. R. (2003). Carotenoids, Immunocompetence, and the information content of sexual colors:an experimental test. Am. 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Behav.48,607-613.","DOI":"10.1006\/anbe.1994.1280"},{"key":"2021042511502216100_REF26","doi-asserted-by":"crossref","unstructured":"Olsson, M., Healey, M., Wapstra, E., Schwartz, T., LeBas, N. and Uller, T. (2007). Mating system variation and morph fluctuations in a polymorphic lizard. Mol. Ecol.16,5307-5315.","DOI":"10.1111\/j.1365-294X.2007.03578.x"},{"key":"2021042511502216100_REF27","doi-asserted-by":"crossref","unstructured":"Omenn, G. S., Goodman, G. E., Thornquist, M. D., Balmes, J.,Cullen, M. R., Glass, A., Keogh, J. P., Meyskens, F. L., Valanis, B.,Williams, J. H. et al. (1996). Effects of a combination of beta carotene and vitamin A on lung cancer and cardiovascular disease. N. Engl. J. Med.334,1150-1155.","DOI":"10.1056\/NEJM199605023341802"},{"key":"2021042511502216100_REF28","doi-asserted-by":"crossref","unstructured":"Peto, R., Buckley, J. D. and Sporn, M. B.(1981). Can dietary beta-carotene materially reduce human cancer rates? 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