{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T16:53:34Z","timestamp":1790873614820,"version":"4.1.0"},"reference-count":36,"publisher":"The Company of Biologists","issue":"8","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2005,4,15]]},"abstract":"<jats:title>SUMMARY<\/jats:title>\n               <jats:p>Previous studies have described the morphology, innervation and O2-chemoreceptive properties of neuroepithelial cells (NECs) of the zebrafish gill filaments. The present work describes the ontogenesis of these cells, and the formation of functional O2-sensing pathways in developing zebrafish. Confocal immunofluorescence was performed on whole-mount gill preparations using antibodies against serotonin (5-HT) and a zebrafish-derived neuronal marker (zn-12) to identify the appearance and innervation of gill NECs during larval stages. NECs were first expressed in gill filament primordia of larvae at 5 days postfertilization (d.p.f.) and were fully innervated by 7 d.p.f. In vivo ventilation frequency analysis revealed that a behavioural response to hypoxia (11.2\u00b12.8 min\u20131) developed in embryos as early as 2 d.p.f., and a significant increase (P&amp;lt;0.05) in the ventilatory response to hypoxia (200.8\u00b123.0 min\u20131) coincided with innervation of NECs of the filaments. In addition, exogenous application of quinidine, a blocker of O2-sensitive background K+ channels in NECs,induced hyperventilation in adults in a dose-dependent manner and revealed the development of a quinidine-sensitive ventilatory response in 7 d.p.f. larvae. This study shows that NEC innervation in the gill filaments may account for the development of a functional O2-sensing pathway and the hyperventilatory response to hypoxia in zebrafish larvae. At earlier stages,however, O2-sensing must occur through another pathway. The possibility that a new type of 5-HT-positive NEC of the gill arches may account for this earlier hypoxic response is discussed.<\/jats:p>","DOI":"10.1242\/jeb.01564","type":"journal-article","created":{"date-parts":[[2005,3,31]],"date-time":"2005-03-31T23:13:04Z","timestamp":1112310784000},"page":"1537-1549","source":"Crossref","is-referenced-by-count":153,"title":["Development of oxygen sensing in the gills of zebrafish"],"prefix":"10.1242","volume":"208","author":[{"given":"Michael G.","family":"Jonz","sequence":"first","affiliation":[{"name":"Department of Biology, McMaster University, 1280 Main Street West,Hamilton, ON, Canada L8S 4K1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Colin A.","family":"Nurse","sequence":"additional","affiliation":[{"name":"Department of Biology, McMaster University, 1280 Main Street West,Hamilton, ON, Canada L8S 4K1"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","reference":[{"key":"2021042510483184500_","doi-asserted-by":"crossref","unstructured":"Barrionuevo, W. 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