{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T22:06:12Z","timestamp":1790892372867,"version":"4.1.0"},"reference-count":51,"publisher":"The Company of Biologists","issue":"24","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2006,12,15]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>Neuroendocrine regulation of cardiac function involves a population of three types of \u03b2-adrenoceptors (ARs). In various mammalian species,\u03b21- and \u03b22-AR stimulation produces an increase in contractility;whereas \u03b23-AR activation mediates negative inotropic effects. At the moment, nothing is known about the physiological role of \u03b23-AR in fish.<\/jats:p><jats:p>Using an isolated working heart preparation, we show that a \u03b23-AR selective agonist BRL37344 (0.1-100 nmol l-1) elicits a dose-dependent negative inotropism in the freshwater eel Anguilla anguilla. This effect was insensitive to the \u03b21\/\u03b22-AR inhibitor nadolol (10 \u03bcmol l-1), but was blocked by the\u03b23-AR-specific antagonist SR59230 (10 nmol l-1). The analysis of the percentage of stroke work (SW) variations, in terms of EC50 values, induced by BRL37344 alone (10 nmol l-1), and in presence of SR59230 (10 nmol l-1), indicated a competitive antagonism of SR59230. In addition to the classic positive inotropism, the non-specific \u03b2 agonist isoproterenol (100 nmol l-1) induced, in 30% of the preparations, a negative inotropic effect that was abrogated by pre-treatment with SR59230, pointing to a \u03b23-mediated pathway. The BRL37344-induced negative inotropic effect was abolished by exposure to a Gi\/o proteins inhibitor pertussis toxin (PTx; 0.01 nmol l-1), suggesting a Gi\/o-dependent mechanism. Using L-N5(l-imino-ethyl)ornithine (L-NIO; 10 \u03bcmol l-1), as a nitric oxide (NO) synthase (NOS) blocker and haemoglobin (Hb; 1 \u03bcmol l-1), as a NO scavenger, we demonstrated that NO signalling is involved in the BRL37344-induced response. Pre-treatment with either an inhibitor of soluble guanylate cyclase (GC) 1H-(1,2,4)oxadiazolo-(4,3-a)quinoxalin-1-one (ODQ; 10 \u03bcmol l-1), or an inhibitor of the cGMP-activated protein kinase (PKG) KT5823 (100 nmol l-1), abolished the \u03b23-dependent negative inotropism,indicating the cGMP-PKG component as a crucial target of NO signalling. Taken together, our findings provide functional evidence for the presence of\u03b23-like adrenoceptors in the eel Anguilla anguilla heart identifying, for the first time in a working fish heart, the\u03b23-AR-dependent negative inotropy discovered in mammals.<\/jats:p>","DOI":"10.1242\/jeb.02595","type":"journal-article","created":{"date-parts":[[2006,12,1]],"date-time":"2006-12-01T22:07:49Z","timestamp":1165010869000},"page":"4966-4973","source":"Crossref","is-referenced-by-count":51,"title":["Beta3-Adrenoceptor in the eel (<i>Anguilla anguilla<\/i>) heart:negative inotropy and NO-cGMP-dependent mechanism"],"prefix":"10.1242","volume":"209","author":[{"given":"S.","family":"Imbrogno","sequence":"first","affiliation":[{"name":"Departments of Pharmaco-Biology, University of Calabria, 87030, Arcavacata di Rende, CS, Italy"},{"name":"Departments of Cell Biology, University of Calabria, 87030, Arcavacata di Rende, CS, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Angelone","sequence":"additional","affiliation":[{"name":"Departments of Pharmaco-Biology, University of Calabria, 87030, Arcavacata di Rende, CS, Italy"},{"name":"Departments of Cell Biology, University of Calabria, 87030, Arcavacata di Rende, CS, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Adamo","sequence":"additional","affiliation":[{"name":"Departments of Cell Biology, University of Calabria, 87030, Arcavacata di Rende, CS, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Pulera\u0300","sequence":"additional","affiliation":[{"name":"Departments of Cell Biology, University of Calabria, 87030, Arcavacata di Rende, CS, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Tota","sequence":"additional","affiliation":[{"name":"Departments of Cell Biology, University of Calabria, 87030, Arcavacata di Rende, CS, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. C.","family":"Cerra","sequence":"additional","affiliation":[{"name":"Departments of Pharmaco-Biology, University of Calabria, 87030, Arcavacata di Rende, CS, Italy"},{"name":"Departments of Cell Biology, University of Calabria, 87030, Arcavacata di Rende, CS, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","reference":[{"key":"2021042509525382600_REF1","doi-asserted-by":"crossref","unstructured":"Ai, T., Horie, M., Obayashi, K. and Sasayama, S.(1998). Accentuated antagonism by angiotensin II on guinea-pig cardiac L-type Ca-currents enhanced by beta-adrenergic stimulation. Pflug. Arch.436,168-174.","DOI":"10.1007\/s004240050619"},{"key":"2021042509525382600_REF2","unstructured":"Angelone, T., Goumon, Y., Cerra, M. C., Metz-boutigue, M. H.,Aunis D. and Tota, B. (2006). The emerging cardio-inhibitory role of the Hippocampal Cholinergic Neurostimulating Peptide (HCNP). J. Pharmacol. Exp. Ther.318, 1-9."},{"key":"2021042509525382600_REF3","doi-asserted-by":"crossref","unstructured":"Arch, J. and Kaumann, A. (1993).\u03b2 3 and atypical \u03b2-adrenoceptors. Med. Res. Rev. 13,663-729.","DOI":"10.1002\/med.2610130604"},{"key":"2021042509525382600_REF4","doi-asserted-by":"crossref","unstructured":"Arch, J. R. and Wilson, S. (1996). Beta 3-adrenoceptors and the regulation of metabolism in adipose tissues. Biochem. Soc. Trans.24,412-418.","DOI":"10.1042\/bst0240412"},{"key":"2021042509525382600_REF5","doi-asserted-by":"crossref","unstructured":"Balligand, J. L. (2000). The beta 3-adrenoceptor: physiological role and potential therapeutic applications. Acta Clin. Belg. 55,209-214.","DOI":"10.1080\/17843286.2000.11754298"},{"key":"2021042509525382600_REF6","unstructured":"Berlan, M., Galitzky, J., Bouquet-Melou, A., Lafontan, M. and Montastruc, J. L. (1993). \u03b23-adrenoceptor increase in cutaneous blood flow in the dog. J. Pharmacol. Exp. Ther.268,1444-1451."},{"key":"2021042509525382600_REF7","doi-asserted-by":"crossref","unstructured":"Bianchetti, A. and Manara, L. (1990). In vitro inhibition of intestinal motility by phenylethanolamines: evidence of atypical\u03b2-adrenoceptors in rat colon. Br. J. Pharmocol. 100,831-839.","DOI":"10.1111\/j.1476-5381.1990.tb14100.x"},{"key":"2021042509525382600_REF8","doi-asserted-by":"crossref","unstructured":"Boivin, B., Lavoie, C., Vaniotis, G., Baragli, A., Villeneuve,L-R., Ethier, N., Trieu, P., Allen, B. G. and He\u0301bert, T. E.(2006). Functional \u03b2-adrenergic receptor signalling on nuclear membranes in adult rat and mouse ventricular cardiomyocytes. Cardiovasc. Res.71,69-78.","DOI":"10.1016\/j.cardiores.2006.03.015"},{"key":"2021042509525382600_REF9","doi-asserted-by":"crossref","unstructured":"Brodde, O. E. (1991). Pathophysiology of the beta-adrenoceptor system in chronic heart failure: consequences for treatment with agonists, partial agonists or antagonists? Eur. Heart J.12F,54-62.","DOI":"10.1093\/eurheartj\/12.suppl_F.54"},{"key":"2021042509525382600_REF10","doi-asserted-by":"crossref","unstructured":"Brodde, O. E., Bruck, H. and Leineweber, K.(2006). Cardiac adrenoceptors: physiological and pathophysiological relevance. J. Pharmacol. Sci.100,323-337.","DOI":"10.1254\/jphs.CRJ06001X"},{"key":"2021042509525382600_REF11","doi-asserted-by":"crossref","unstructured":"Chamberlain, P. D., Jennings, K. H. and Paul, F.(1999). The tissue distribution of the human\u03b2 3-adrenoceptor studied using a monoclonal antibody: direct evidence of the \u03b23-adrenergic in human adipose tissue, atrium and skeletal muscle. Int. J. Obes. Relat. Metab. Disord. 10,1057-1065.","DOI":"10.1038\/sj.ijo.0801039"},{"key":"2021042509525382600_REF12","doi-asserted-by":"crossref","unstructured":"Emorine, L. J., Marullo, S., Briend-Sutren, M. M., Patey, G.,Tate, K., Delavier-Klutchko, C. and Strosberg, A. D. (1989). Molecular characterization of the human beta 3-adrenergic receptor. Science245,1118-1121.","DOI":"10.1126\/science.2570461"},{"key":"2021042509525382600_REF13","doi-asserted-by":"crossref","unstructured":"Evans, B. A., Papaioannou, M., Bonazzi, V. R. and Summers, R. J. (1996). Expression of beta 3-adrenoceptor mRNA in rat tissues. Br. J. Pharmacol.117,210-216.","DOI":"10.1111\/j.1476-5381.1996.tb15176.x"},{"key":"2021042509525382600_REF14","doi-asserted-by":"crossref","unstructured":"Galitzky, J., Reverte, M., Portillo, M., Carpene, C., Lafontan,M. and Berlan, M. (1993). Coexistence of beta 1-, beta 2-,and beta 3-adrenoceptors in dog fat cells and their differential activation by catecholamines. Am. J. Physiol.264,403-412.","DOI":"10.1152\/ajpendo.1993.264.3.E403"},{"key":"2021042509525382600_REF15","doi-asserted-by":"crossref","unstructured":"Gamperl, A. K., Wilkinson, M. and Boutilier, R. G.(1994). Betaadrenoreceptors in the trout (Oncorhynchus mykiss) heart: characterization, quantification, and effects of repeated catecholamine exposure. Gen. Comp. Endocrinol. 95,259-272.","DOI":"10.1006\/gcen.1994.1123"},{"key":"2021042509525382600_REF16","doi-asserted-by":"crossref","unstructured":"Gauthier, C., Tavernier, G., Charpentier, F., Langin, D. and Le Marec, H. (1996). Functional \u03b23-adrenoceptor in the human heart. J. Clin. Invest.98,556-562.","DOI":"10.1172\/JCI118823"},{"key":"2021042509525382600_REF17","doi-asserted-by":"crossref","unstructured":"Gauthier, C., Leblais, V., Trochu, J. N., Balligand, J. L. and Le Marec, H. (1998). The negative inotropic effect of\u03b2 3-adrenoceptor stimulation is mediated by activation of a nitric oxide synthase pathway in human ventricle. J. Clin. Invest. 102,1377-1384.","DOI":"10.1172\/JCI2191"},{"key":"2021042509525382600_REF18","unstructured":"Gauthier, C., Tavernier, G., Trochu, J. N., Leblais, V.,Laurent, K., Langin, D., Escande, D. and Le Marec, H. (1999). Interspecies differences in the cardiac negative inotropic effects of\u03b2 3-adrenoceptor agonist. J. Pharmacol. Exp. Ther.290,687-693."},{"key":"2021042509525382600_REF19","doi-asserted-by":"crossref","unstructured":"Gauthier, C., Langin, D. and Balligand, J.-L.(2000). \u03b23-Adrenoceptors in the cardiovascular system. J. Clin. Invest. 21,426-430.","DOI":"10.1016\/S0165-6147(00)01562-5"},{"key":"2021042509525382600_REF20","doi-asserted-by":"crossref","unstructured":"Gros, J., Manning, B. S., Pietri-Rouxel, F., Guillaume, J. L.,Drumare, M. F. and Strosberg, A. D. (1998). Site-directed mutagenesis of the human beta3-adrenoceptor-transmembrane residues involved in ligand binding and signal transduction. Eur. J. Biochem.251,590-596.","DOI":"10.1046\/j.1432-1327.1998.2510590.x"},{"key":"2021042509525382600_REF21","doi-asserted-by":"crossref","unstructured":"Hare, J. M., Givertz, M. M., Creager, M. A. and Colucci, W. S. (1998). Increased sensitivity to nitric oxide synthase inhibition in patients with heart failure: potentiation of beta-adrenergic inotropic responsiveness. Circulation97,161-166.","DOI":"10.1161\/01.CIR.97.2.161"},{"key":"2021042509525382600_REF22","doi-asserted-by":"crossref","unstructured":"Hove-Madsen, L., Mery, P. F., Jurevicius, J., Skeberdis, A. V. and Fishmeister, R. (1996). Regulation of myocardial calcium channels by cyclic AMP metabolism. Basic Res. Cardiol. 91, 1-8.","DOI":"10.1007\/BF00795355"},{"key":"2021042509525382600_REF23","doi-asserted-by":"crossref","unstructured":"Imbrogno, S., De Iuri, L., Mazza, R. and Tota, B.(2001). Nitric oxide modulates cardiac performance in the heart of Anguilla anguilla.J. Exp. Biol.204,1719-1727.","DOI":"10.1242\/jeb.204.10.1719"},{"key":"2021042509525382600_REF24","doi-asserted-by":"crossref","unstructured":"Imbrogno, S., Cerra, M. C. and Tota, B. (2003). Angiotensin II-induced inotropism requires an endocardial endothelium-nitric oxide mechanism in the in-vitro heart of Anguilla anguilla.J. Exp. Biol.206,2675-2684.","DOI":"10.1242\/jeb.00468"},{"key":"2021042509525382600_REF25","doi-asserted-by":"crossref","unstructured":"Imbrogno, S., Angelone, T., Corti, A., Adamo, C., Helle, K. B. and Tota, B. (2004). Influence of vasostatins, the chromogranin A-derived peptides, on the working heart of the eel (Anguilla anguilla): negative inotropy and mechanism of action. Gen. Comp. Endocrinol. 139,20-28.","DOI":"10.1016\/j.ygcen.2004.07.008"},{"key":"2021042509525382600_REF26","doi-asserted-by":"crossref","unstructured":"Ishikawa, Y. and Homcy, C. J. (1997). The adenylyl cyclases as integrators of transmembrane signal transduction. Circ. Res.80,297-304.","DOI":"10.1161\/01.RES.80.3.297"},{"key":"2021042509525382600_","doi-asserted-by":"crossref","unstructured":"Kaumann, A. J. (1997). Four beta-adrenoceptor subtypes in the mammalian heart. Trends Pharmacol. Sci.18,70-76.","DOI":"10.1016\/S0165-6147(96)01033-4"},{"key":"2021042509525382600_REF28","doi-asserted-by":"crossref","unstructured":"Kitamura, T., Onishi, K., Dohi, K., Okinaka, T., Isaka, N. and Nakano, T. (2000).The negative inotropic effect of beta3-adrenoceptor stimulation in the beating guinea pig heart. J. Cardiovasc. Pharmacol.35,786-790.","DOI":"10.1097\/00005344-200005000-00016"},{"key":"2021042509525382600_REF29","doi-asserted-by":"crossref","unstructured":"Lands, A., Arnold, A., Auliff, J. M. and Luduena, F.(1967). Differentiation of receptor systems activated by simpathomimetic amines. Nature214,597-598.","DOI":"10.1038\/214597a0"},{"key":"2021042509525382600_REF30","doi-asserted-by":"crossref","unstructured":"Langin, D., Portillo, M., Saulnier-Blache, J. and Lafontan,M. (1991). Coexistences of three \u03b2-adrenergic receptors subtypes in white fat cells of various mammalian species. Eur. J. Pharmacol.199,291-301.","DOI":"10.1016\/0014-2999(91)90492-9"},{"key":"2021042509525382600_REF31","doi-asserted-by":"crossref","unstructured":"McLaughlin, D. and MacDonald, A. (1990). Evidence for the existence of `atypical' \u03b2-adrenoceptors(\u03b23-adrenoceptors) mediating relaxation in the rat distal colon. Br. J. Pharmacol.101,569-574.","DOI":"10.1111\/j.1476-5381.1990.tb14122.x"},{"key":"2021042509525382600_REF32","doi-asserted-by":"crossref","unstructured":"Me\u0301ry, P. F., Lohmann, S. M., Walter, U. and Fischmeister,R. (1991). Ca2+ current is regulated by cGMP-dependent protein Kinase in mammalian cardiac myocite. Proc. Natl. Acad. Sci. USA88,1197-1201.","DOI":"10.1073\/pnas.88.4.1197"},{"key":"2021042509525382600_REF33","unstructured":"Nickerson, J. G., Dugan, S. G., Drouin, G., Perry, S. F. and Moon, T. W. (2003). Activity of the unique beta-adrenergic Na+\/H+ exchanger in trout erythrocytes is controlled by a novel beta3-AR subtype. Am. J. Physiol.285,R526-R535."},{"key":"2021042509525382600_REF34","doi-asserted-by":"crossref","unstructured":"Norman, B. and Learthard, H. (1990). Evidence that an atypical \u03b2-adrenoceptor mediates the inhibition of spontaneous rhythmical contractions of rabbit isolated jejunum induced by ritodrine and salbutamol. Br. J. Pharmacol.101, 27-30.","DOI":"10.1111\/j.1476-5381.1990.tb12083.x"},{"key":"2021042509525382600_REF35","doi-asserted-by":"crossref","unstructured":"Pelat, M., Verwaerde, P., Galitzky, J., Lafontanm, M., Berlan,M., Senard, J. M. and Montastruc, J. L. (2003). High isoproterenol doses are required to activate beta3-adrenoceptor-mediated functions in dogs. J. Pharmacol. Exp. Ther.304,246-53.","DOI":"10.1124\/jpet.102.040691"},{"key":"2021042509525382600_REF36","doi-asserted-by":"crossref","unstructured":"Sennit, M. V., Kaumann, A. J., Molenaar, P., Beeley, L. J.,Young, P. W., Kelly, J., Chapman, H., Henson, S. M., Berg, J. M., Dean, D. K. et al. (1998). The contribution of classical(\u03b2\u00bd-) and atypical \u03b2-adrenoceptor to the stimulation of human white adipocyte lipolysis and right atrial appendace contraction by novel \u03b23-adrenoceptors agonists of differing selectivities. J. Pharmacol. Exp. Ther.285,1084-1095.","DOI":"10.1016\/S0022-3565(24)37506-8"},{"key":"2021042509525382600_REF37","unstructured":"Shen, Y. T., Cervoni, P., Claus, T. and Vatner, S. F.(1996). Differences in beta 3-adrenergic receptor cardiovascular regulation in conscious primates, rats and dogs. J. Pharmacol. Exp. Ther.278,1435-1443."},{"key":"2021042509525382600_REF38","unstructured":"Skeberdis, V. A. (2004). Structure and function of \u03b23-adrenergic receptors. Medicina(Kaunas)40,407-412."},{"key":"2021042509525382600_REF39","doi-asserted-by":"crossref","unstructured":"Sterin-Borda, L., Bernabeo, G., Ganzinelli, S., Joensen, L. and Borda, E. (2006). Role of nitric oxide\/cyclic GMP and cyclic AMP in beta3 adrenoceptor-chronotropic response. J. Mol. Cell Cardiol.40,580-588.","DOI":"10.1016\/j.yjmcc.2006.01.017"},{"key":"2021042509525382600_REF40","doi-asserted-by":"crossref","unstructured":"Strosberg, A. D. and Pietri-Rouxel, F. (1996). Function and regulation of the beta 3-adrenoceptor. Trends Pharmacol. Sci.17,373-381.","DOI":"10.1016\/S0165-6147(96)80011-3"},{"key":"2021042509525382600_REF41","unstructured":"Tavernier, G., Galitzky, J., Bousquet-Melon, A., Montastruc, J. L. and Berlan, M. (1992). The positive chronotropic effet induced by BRL37344 and CGP12177, two \u03b23-adrenergic agonist,does not involve cardiac \u03b2-adrenoceptors but reflex mechanism. J. Pharmacol. Exp. Ther.91,344-349."},{"key":"2021042509525382600_REF42","doi-asserted-by":"crossref","unstructured":"Tavernier, G., Toumaniantz, G., Erfanian, M., Heymann, M.-F.,Laurent, K., Langin, D. and Gauthier, C. (2003).\u03b23-adrenergic stimulation produces a decrease of cardiac contractility ex vivo in mice overexpressing the human \u03b23-adrenergic receptor. Cardiovasc. Res.59,288-296.","DOI":"10.1016\/S0008-6363(03)00359-6"},{"key":"2021042509525382600_REF43","unstructured":"Tota, B., Imbrogno, S., Mannarino, C. and Mazza, R.(2004). Vasostatins and negative inotropy uin vertebrate hearts. Curr. Med. Chem.4,195-201."},{"key":"2021042509525382600_REF44","doi-asserted-by":"crossref","unstructured":"Tota, B., Amelio, D., Pellegrino, D., Ip, Y. K. and Cerra, M. C. (2005). NO modulation of myocardial performance in fish hearts. Comp. Biochem. Physiol.142A,164-177.","DOI":"10.1016\/j.cbpb.2005.04.019"},{"key":"2021042509525382600_REF45","doi-asserted-by":"crossref","unstructured":"Trochu, J. N., Leblais, V., Rautureau, Y., Beverelli, F., Le Marec, H., Berdeaux, A. and Gauthier, C. (1999). Beta 3-adrenoceptor stimulation induces vasorelaxation mediated essentially by endothelium-derived nitric oxide in rat thoracic aorta. Br. J. Pharmacol.128,69-76.","DOI":"10.1038\/sj.bjp.0702797"},{"key":"2021042509525382600_REF46","doi-asserted-by":"crossref","unstructured":"Varghese, P., Harrison, R. H., Lofthouse, R. A., Georgakopoulos,D., Berkowitz, D. E. and Hare, M. (2000).\u03b2 3-adrenoceptor deficiency blocks nitric oxide-dependent inhibition of myocardial contractility. J. Clin. Invest. 106,697-703.","DOI":"10.1172\/JCI9323"},{"key":"2021042509525382600_REF47","unstructured":"Wheeldon, N. M., McDevitt, D. G. and Lipworth, B. J.(1993). Investigation of putative cardiac beta 3-adrenoceptors in man. Q. J. Med.86,255-261."},{"key":"2021042509525382600_REF48","doi-asserted-by":"crossref","unstructured":"Wheeldon, N. M., McDevitt, D. G. and Lipworth, B. J.(1994). Cardiac effects of the beta 3-adrenoceptor agonist BRL35135 in man. Br. J. Clin. Pharmacol.37,363-369.","DOI":"10.1111\/j.1365-2125.1994.tb04290.x"},{"key":"2021042509525382600_REF49","doi-asserted-by":"crossref","unstructured":"Xiao, R. P., Cheng, H., Zhou, Y. Y., Kuschel, M. and Lakatta, E. G. (1999). Recent advances in cardiac \u03b22-adrenergic signal transduction. Circ Res. 85,1092-1100.","DOI":"10.1161\/01.RES.85.11.1092"},{"key":"2021042509525382600_REF50","doi-asserted-by":"crossref","unstructured":"Yoshida, T., Hiraoka, N. and Kondo, M. (1991). Effects of a beta 3-adrenoceptor agonist, BRL 26830A, on insulin and glucagon release in mice. Endocrinol. Jpn.38,641-646.","DOI":"10.1507\/endocrj1954.38.641"},{"key":"2021042509525382600_REF51","doi-asserted-by":"crossref","unstructured":"Zhang, Z. S., Cheng, H. J., Onishi, K., Ohte, N., Wannenburg, T. and Cheng, C. P. (2005). Enhanced inhibition of L-type Ca2+ current by beta3-adrenergic stimulation in failing rat heart. J. Pharmacol. Exp. Ther.315,1203-1211.","DOI":"10.1124\/jpet.105.089672"}],"container-title":["Journal of Experimental Biology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/journals.biologists.com\/jeb\/article-pdf\/209\/24\/4966\/1258133\/4966.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/journals.biologists.com\/jeb\/article-pdf\/209\/24\/4966\/1258133\/4966.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,12]],"date-time":"2025-01-12T06:28:23Z","timestamp":1736663303000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.biologists.com\/jeb\/article\/209\/24\/4966\/16417\/Beta3-Adrenoceptor-in-the-eel-Anguilla-anguilla"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,12,15]]},"references-count":51,"journal-issue":{"issue":"24","published-print":{"date-parts":[[2006,12,15]]}},"URL":"https:\/\/doi.org\/10.1242\/jeb.02595","relation":{},"ISSN":["1477-9145","0022-0949"],"issn-type":[{"value":"1477-9145","type":"electronic"},{"value":"0022-0949","type":"print"}],"subject":[],"published":{"date-parts":[[2006,12,15]]}}}