{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T16:12:59Z","timestamp":1773418379335,"version":"3.50.1"},"reference-count":39,"publisher":"The Company of Biologists","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2007,1,1]]},"abstract":"<jats:title>SUMMARY<\/jats:title>\n               <jats:p>In reptiles, accumulating evidence suggests that nitric oxide (NO) induces a potent relaxation in the systemic vasculature. However, very few studies have examined the source from which NO is derived. Therefore, the present study used both anatomical and physiological approaches to establish whether NO-mediated vasodilation is via an endothelial or neural NO pathway in the large arteries of the estuarine crocodile Crocodylus porosus. Specific endothelial nitric oxide synthase (NOS) staining was observed in aortic endothelial cells following nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d) histochemistry and endothelial NOS immunohistochemistry (IHC), suggesting that an endothelial NO pathway is involved in vascular control. This finding was supported by in vitroorgan bath physiology, which demonstrated that the relaxation induced by acetylcholine (10-5 mol l-1) was abolished in the presence of the NOS inhibitor, N-omega-nitro-l-arginine(l-NNA; 10-4 mol l-1), the soluble guanylyl cyclase inhibitor, 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ;10-5 mol l-1), or when the endothelium was removed. Interestingly, evidence for a neural NO pathway was also identified in large arteries of the crocodile. Neural NOS was located in perivascular nerves of the major blood vessels following NADPH-d histochemistry and neural NOS IHC and in isolated aortic rings, l-NNA and ODQ, but not the removal of the endothelium, abolished the relaxation effect of the neural NOS agonist,nicotine (3\u00d710-4 mol l-1). Thus, we conclude that the large arteries of C. porosus are potentially regulated by NO-derived from both endothelial and neural NOS.<\/jats:p>","DOI":"10.1242\/jeb.02620","type":"journal-article","created":{"date-parts":[[2006,12,14]],"date-time":"2006-12-14T18:13:03Z","timestamp":1166119983000},"page":"129-137","source":"Crossref","is-referenced-by-count":15,"title":["Dual mechanisms for nitric oxide control of large arteries in the estuarine crocodile <i>Crocodylus porosus<\/i>"],"prefix":"10.1242","volume":"210","author":[{"given":"Brad R. S.","family":"Broughton","sequence":"first","affiliation":[{"name":"School of Life and Environmental Sciences, Deakin University,Geelong, Victoria, 3217, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John A.","family":"Donald","sequence":"additional","affiliation":[{"name":"School of Life and Environmental Sciences, Deakin University,Geelong, Victoria, 3217, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","reference":[{"key":"2021042513175655400_REF1","doi-asserted-by":"crossref","unstructured":"Anderson, C. R., Furness, J. B., Woodman, H., Edwards, S. L.,Crack, P. J. and Smith, A. I. (1995). Characterisation of neurons with nitric oxide synthase immunoreactivity that project to prevertebral ganglia. J. Auton. Nerv. Syst.52,107-116.","DOI":"10.1016\/0165-1838(94)00150-I"},{"key":"2021042513175655400_REF2","doi-asserted-by":"crossref","unstructured":"Axelsson, M., Olsson, C., Gibbins, I., Holmgren, S. and Franklin, C. E. (2001). Nitric oxide, a potent vasodilator of the aortic anastomosis in the estuarine crocodile, Crocodylus porosus.Gen. Comp. Endocrinol.122,198-204.","DOI":"10.1006\/gcen.2001.7627"},{"key":"2021042513175655400_REF3","doi-asserted-by":"crossref","unstructured":"Beesley, J. E. (1995). Histochemical methods for detecting nitric oxide synthase. Histochem. J.27,757-769.","DOI":"10.1007\/BF02388301"},{"key":"2021042513175655400_REF4","doi-asserted-by":"crossref","unstructured":"Broughton, B. R. S. and Donald, J. A. (2002). Nitric oxide regulation of the central aortae of the toad Bufo marinus occurs independently of the endothelium. J. Exp. Biol.205,3093-3100.","DOI":"10.1242\/jeb.205.19.3093"},{"key":"2021042513175655400_REF5","doi-asserted-by":"crossref","unstructured":"Broughton, B. R. S. and Donald, J. A. (2005). Nitric oxide control of large veins in the toad, Bufo marinus.J. Comp. Physiol. 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