{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T22:57:09Z","timestamp":1784242629731,"version":"3.55.0"},"reference-count":37,"publisher":"The Company of Biologists","issue":"22","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2009,11,15]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>The role of serotonin in the regulation of larval Aedes aegyptihemolymph composition was investigated in vivo using two reuptake inhibitors (SSRIs), alaproclate HCl and 6-nitroquipazine maleate, and the receptor antagonist methiothepin mesylate. Larvae were placed in media differing in pH and salinity in the presence and absence of serotonergic agents. The toxicity of each agent was strongly influenced by ambient pH. For each agent, toxicity was negligible in acidic media, intermediate in neutral media and greatest in alkaline media. By contrast, toxicity of all agents was independent of salinity. No effects on mass-specific body water or hemolymph volume were observed whereas hemolymph osmotic pressure, Na+concentrations and pH differed significantly among treatments. 6-nitroquipazine caused a decrease in Na+ from 115\u00b11.7 to 103\u00b10.9 mmol l\u20131, and alaproclate caused alkalosis of the hemolymph from pH 7.55\u00b10.026 to pH 7.72\u00b10.044. Methiothepin decreased hemolymph osmotic pressure from 329\u00b19.9 to 304\u00b18.8 and showed the greatest overall toxicity. Control larvae excreted net base in pH 4 media (1.4 \u03bcmol g\u20131 h\u20131) and net acid in pH 7 (1.2 \u03bcmol g\u20131 h\u20131) and pH 11 (5.1\u03bcmol g\u20131 h\u20131) media. In pH 4 media,alaproclate and methiothepin caused a shift to net H+ excretion(1.1 and 1.5 \u03bcmol g\u20131 h1, respectively) whereas these agents did not influence acid excretion rates in pH 7 or pH 11 media. The hypothesis that serotonin is involved in hemolymph acid\u2013base balance is discussed.<\/jats:p>","DOI":"10.1242\/jeb.032086","type":"journal-article","created":{"date-parts":[[2009,10,30]],"date-time":"2009-10-30T17:59:28Z","timestamp":1256925568000},"page":"3728-3736","source":"Crossref","is-referenced-by-count":10,"title":["Effects of serotonergic agents on survival and hemolymph composition of the larval mosquito<i>Aedes aegypti<\/i>(Diptera: Culicidae, L.)<i>in vivo<\/i>: does serotonin regulate hemolymph acid\u2013base homeostasis?"],"prefix":"10.1242","volume":"212","author":[{"given":"T. M.","family":"Clark","sequence":"first","affiliation":[{"name":"Indiana University South Bend, 1700 Mishawaka Avenue, South Bend, IN 46634-1700, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. L.","family":"Lawecki","sequence":"additional","affiliation":[{"name":"Indiana University South Bend, 1700 Mishawaka Avenue, South Bend, IN 46634-1700, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. J.","family":"Shepherd","sequence":"additional","affiliation":[{"name":"Indiana University South Bend, 1700 Mishawaka Avenue, South Bend, IN 46634-1700, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. N.","family":"Hirschler","sequence":"additional","affiliation":[{"name":"Indiana University South Bend, 1700 Mishawaka Avenue, South Bend, IN 46634-1700, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T. R.","family":"Samandu","sequence":"additional","affiliation":[{"name":"Indiana University South Bend, 1700 Mishawaka Avenue, South Bend, IN 46634-1700, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","reference":[{"key":"2021042512110383500_REF1","doi-asserted-by":"crossref","unstructured":"Barrett, F. M., Orchard, I. and Tebrugge, V.(1993). Characteristics of serotonin-induced cyclic AMP elevation in the integument and anterior midgut of the blood-feeding bug, Rhodnius prolixus.J. Insect Physiol.39,581-587.","DOI":"10.1016\/0022-1910(93)90040-X"},{"key":"2021042512110383500_REF2","doi-asserted-by":"crossref","unstructured":"Berenbaum, M.R. (1980). Adaptive significance of midgut pH in larval Lepidoptera. Am. Nat.115,138-146.","DOI":"10.1086\/283551"},{"key":"2021042512110383500_REF3","unstructured":"Cady, C. and Hagedorn, H. H. (1998). The effect of putative diuretic factors on in vivo urine production in the mosquito, Aedes aegypti.J. 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