{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T15:30:08Z","timestamp":1774452608731,"version":"3.50.1"},"reference-count":44,"publisher":"The Company of Biologists","issue":"13","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2011,7,1]]},"abstract":"<jats:title>SUMMARY<\/jats:title>\n               <jats:p>Both pregnancy and lactation are associated with hyperphagia, and circulating leptin levels are elevated during pregnancy but decreased during lactation in Brandt's voles, Lasiopodomys brandtii. Previous findings suggest that impaired leptin sensitivity contributes to hyperphagia during pregnancy. The present study aimed to examine whether the decreased circulating leptin level and\/or hypothalamic leptin sensitivity contributed to the hyperphagia during lactation in Brandt's voles. The serum leptin level and mRNA expression of the long form of the leptin receptor (Ob-Rb), suppressor-of-cytokine-signalling-3 (SOCS-3), neuropeptide Y (NPY), agouti-related protein (AgRP), pro-opiomelanocortin (POMC) and cocaine- and amphetamine-regulated transcript (CART) in the hypothalamus were examined on dioestrous, day 5, day 17 of lactation and day 27 (1 week after weaning) in Brandt's voles. Compared with controls, hypothalamic Ob-Rb and SOCS-3 mRNA expression was not significantly changed during lactation. The serum leptin level was significantly lower in lactating females than in the non-reproductive group. Hypothalamic NPY and AgRP mRNA expression significantly increased whereas POMC mRNA expression was significantly decreased during lactation compared with controls. However, there were no significant changes in hypothalamic CART mRNA expression. Food intake was positively correlated with NPY and AgRP mRNA expression but negatively correlated with POMC mRNA expression during lactation. These data suggest that hyperphagia during lactation was associated with low leptin levels, but not impaired leptin sensitivity, and that the hypothalamic neuropeptides NPY, AgRP and POMC are involved in mediating the role of leptin in food intake regulation in lactating Brandt's voles.<\/jats:p>","DOI":"10.1242\/jeb.054056","type":"journal-article","created":{"date-parts":[[2011,6,8]],"date-time":"2011-06-08T18:53:55Z","timestamp":1307559235000},"page":"2242-2247","source":"Crossref","is-referenced-by-count":5,"title":["Hypothalamic neuropeptides, not leptin sensitivity, contributes to the hyperphagia in lactating Brandt's voles, <i>Lasiopodomys brandtii<\/i>"],"prefix":"10.1242","volume":"214","author":[{"given":"Jian-Guo","family":"Cui","sequence":"first","affiliation":[{"name":"State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China"},{"name":"Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gang-Bing","family":"Tang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"De-Hua","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","reference":[{"key":"2021042506155833800_R1","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1006\/frne.2000.0197","article-title":"Leptin regulation of neuroendocrine systems","volume":"21","author":"Ahima","year":"2000","journal-title":"Front. Neuroendocrinol."},{"key":"2021042506155833800_R2","doi-asserted-by":"crossref","first-page":"4996","DOI":"10.1210\/en.2005-0755","article-title":"Suppression of prolactin-induced signal transducer and activator of transcription 5b signaling and induction of suppressors of cytokine signaling messenger ribonucleic acid in the hypothalamic arcuate nucleus of the rat during late pregnancy and lactation","volume":"147","author":"Anderson","year":"2006","journal-title":"Endocrinology"},{"key":"2021042506155833800_R3","doi-asserted-by":"crossref","first-page":"14563","DOI":"10.1074\/jbc.275.19.14563","article-title":"Activation of downstream signals by the long form of the leptin receptor","volume":"275","author":"Banks","year":"2000","journal-title":"J. Biol. Chem."},{"key":"2021042506155833800_R4","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1016\/S0005-2760(97)00079-9","article-title":"Lipid metabolism in the lactating mammary gland","volume":"1347","author":"Barber","year":"1997","journal-title":"Biochim. Biophys. Acta."},{"key":"2021042506155833800_R5","doi-asserted-by":"crossref","first-page":"40649","DOI":"10.1074\/jbc.M007577200","article-title":"SOCS3 mediates feedback inhibition of the leptin receptor via Tyr985","volume":"275","author":"Bj\u00f8rb\u00e6k","year":"2000","journal-title":"J. Biol. Chem."},{"key":"2021042506155833800_R6","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1055\/s-0028-1093890","article-title":"Diurnal changes in the concentrations of plasma lipids, sugars, insulin and corticosterone in rats fed diets containing various carbohydrates","volume":"6","author":"Bruckdorfer","year":"1974","journal-title":"Horm. Metab. Res."},{"key":"2021042506155833800_R7","doi-asserted-by":"crossref","first-page":"2645","DOI":"10.1210\/endo.140.6.6829","article-title":"Altered expression of agouti-related protein and its colocalization with neuropeptide Y in the arcuate nucleus of the hypothalamus during lactation","volume":"140","author":"Chen","year":"1999","journal-title":"Endocrinology"},{"key":"2021042506155833800_R8","doi-asserted-by":"crossref","first-page":"637","DOI":"10.1111\/j.1365-2826.2004.01213.x","article-title":"Role of leptin in orexigenic neuropeptide expression during lactation in rats","volume":"16","author":"Crowley","year":"2004","journal-title":"J. Neuroendocrinol."},{"key":"2021042506155833800_R9","doi-asserted-by":"crossref","first-page":"R447","DOI":"10.1152\/ajpregu.00121.2010","article-title":"Effects of leptin infusion during peak lactation on food intake, body composition, litter growth and maternal neuroendocrine status in female Brandt\u2019s voles (Lasiopodomys brandtii)","volume":"300","author":"Cui","year":"2011","journal-title":"Am. J. Physiol. Regul. Integr. Comp. Physiol."},{"key":"2021042506155833800_R10","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1677\/joe.0.1760193","article-title":"Regulation of serum leptin and its role in the hyperphagia of lactation in the rat","volume":"176","author":"Denis","year":"2003","journal-title":"J. Endocrinol."},{"key":"2021042506155833800_R11","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1677\/joe.0.1780225","article-title":"Lactation modulates diurnal expression profiles of specific leptin receptor isoforms in the rat hypothalamus","volume":"178","author":"Denis","year":"2003","journal-title":"J. Endocrinol."},{"key":"2021042506155833800_R12","doi-asserted-by":"crossref","first-page":"1827","DOI":"10.1172\/JCI9842","article-title":"Two defects contribute to hypothalamic leptin resistance in mice with diet-induced obesity","volume":"105","author":"El-Haschimi","year":"2000","journal-title":"J. Clin. Invest."},{"key":"2021042506155833800_R13","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1677\/joe.0.1560299","article-title":"Effects of food restriction on the responses of the mammary gland and adipose tissue to prolactin and growth hormone in the lactating rat","volume":"156","author":"Flint","year":"1998","journal-title":"J. Endocrinol."},{"key":"2021042506155833800_R14","doi-asserted-by":"crossref","first-page":"763","DOI":"10.1038\/27376","article-title":"Leptin and the regulation of body weight in mammals","volume":"395","author":"Friedman","year":"1998","journal-title":"Nature"},{"key":"2021042506155833800_R15","doi-asserted-by":"crossref","first-page":"734","DOI":"10.1038\/nm1072","article-title":"Enhanced leptin sensitivity and attenuation of diet-induced obesity in mice with haploinsufficiency of Socs3","volume":"10","author":"Howard","year":"2004","journal-title":"Nat. Med."},{"key":"2021042506155833800_R16","doi-asserted-by":"crossref","first-page":"798","DOI":"10.1006\/bbrc.1997.7750","article-title":"The placenta is not the main source of leptin production in pregnant rat: gestational profile of leptin in plasma and adipose tissues","volume":"240","author":"Kawai","year":"1997","journal-title":"Biochem. Biophys. Res. 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Mammal."},{"key":"2021042506155833800_R24","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1007\/s00360-006-0141-4","article-title":"Ultradian rhythms and the nutritional importance of caecotrophy in captive Brandt\u2019s voles (Lasiopodomys brandtii)","volume":"177","author":"Liu","year":"2007","journal-title":"J. Comp. Physiol. B"},{"key":"2021042506155833800_R25","doi-asserted-by":"crossref","first-page":"739","DOI":"10.1038\/nm1071","article-title":"Socs3 deficiency in the brain elevates leptin sensitivity and confers resistance to diet-induced obesity","volume":"10","author":"Mori","year":"2004","journal-title":"Nat. Med."},{"key":"2021042506155833800_R26","doi-asserted-by":"crossref","first-page":"4880","DOI":"10.1210\/en.2004-0726","article-title":"Region-specific leptin resistance within the hypothalamus of diet-induced obese mice","volume":"145","author":"Munzberg","year":"2004","journal-title":"Endocrinology"},{"key":"2021042506155833800_R27","doi-asserted-by":"crossref","first-page":"642","DOI":"10.1007\/s00018-004-4432-1","article-title":"Leptin receptor action and mechanisms of leptin resistance","volume":"62","author":"Munzberg","year":"2005","journal-title":"Cell. Mol. Life Sci."},{"key":"2021042506155833800_R28","doi-asserted-by":"crossref","first-page":"548","DOI":"10.1016\/S0028-3908(02)00003-5","article-title":"Central leptin gene delivery evokes persistent leptin signal transduction in young and aged-obese rats but physiological responses become attenuated over time in aged-obese rats","volume":"42","author":"Scarpace","year":"2002","journal-title":"Neuropharmacology"},{"key":"2021042506155833800_R29","first-page":"R314","article-title":"Body composition, food intake, and brown fat thermogenesis in pregnant Djungarian hamsters","volume":"253","author":"Schneider","year":"1987","journal-title":"Am. J. Physiol."},{"key":"2021042506155833800_R30","doi-asserted-by":"crossref","first-page":"661","DOI":"10.1038\/35007534","article-title":"Central nervous system control of food intake","volume":"404","author":"Schwartz","year":"2000","journal-title":"Nature"},{"key":"2021042506155833800_R31","doi-asserted-by":"crossref","first-page":"1258","DOI":"10.1210\/endo.133.3.8365368","article-title":"Lactation alters neuropeptide-Y and proopiomelanocortin gene expression in the arcuate nucleus of the rat","volume":"133","author":"Smith","year":"1993","journal-title":"Endocrinology"},{"key":"2021042506155833800_R32","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1016\/S0091-3022(02)00002-X","article-title":"Integration of the regulation of reproductive function and energy balance: lactation as a model","volume":"23","author":"Smith","year":"2002","journal-title":"Front. Neuroendocrinol."},{"key":"2021042506155833800_R33","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1016\/S0306-4565(03)00051-2","article-title":"Metabolism and thermoregulation in the striped hamster Cricetulus barabensis","volume":"28","author":"Song","year":"2003","journal-title":"J. Therm. Biol."},{"key":"2021042506155833800_R34","first-page":"95","article-title":"Leptin and its receptors in the course of pregnancy in the rat","volume":"17","author":"Szczepankiewicz","year":"2006","journal-title":"Int. J. Mol. Med."},{"key":"2021042506155833800_R35","doi-asserted-by":"crossref","first-page":"1038","DOI":"10.1111\/j.1365-2826.2008.01764.x","article-title":"Hypothalamic suppressor of cytokine signalling 3 mRNA is elevated and pro-opiomelanocortin mRNA is reduced during pregnancy in Brandt\u2019s voles (Lasiopodomys brandtii)","volume":"20","author":"Tang","year":"2008","journal-title":"J. Neuroendocrinol."},{"key":"2021042506155833800_R36","doi-asserted-by":"crossref","first-page":"R1293","DOI":"10.1152\/ajpregu.00185.2009","article-title":"The role for hypoleptinemia in cold adaptation in Brandt\u2019s voles","volume":"297","author":"Tang","year":"2009","journal-title":"Am. J. Physiol. Regul. Integr. Comp. Physiol."},{"key":"2021042506155833800_R37","doi-asserted-by":"crossref","first-page":"826","DOI":"10.1042\/bst0130826","article-title":"Brown adipose tissue thermogenesis and the energetics of pregnancy and lactation in rodents","volume":"13","author":"Trayhurn","year":"1985","journal-title":"Biochem. Soc. Trans."},{"key":"2021042506155833800_R38","doi-asserted-by":"crossref","first-page":"1185","DOI":"10.1210\/en.2003-1382","article-title":"Photoperiodic regulation of leptin sensitivity in the Siberian hamster, Phodopus sungorus, is reflected in arcuate nucleus SOCS-3 (suppressor of cytokine signaling) gene expression","volume":"145","author":"Tups","year":"2004","journal-title":"Endocrinology"},{"key":"2021042506155833800_R39","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1111\/j.1365-2826.2005.01394.x","article-title":"The suppressor of cytokine signalling 3, SOCS3, may be one critical modulator of seasonal body weight changes in the Siberian hamster, Phodopus sungorus","volume":"18","author":"Tups","year":"2006","journal-title":"J. Neuroendocrinol."},{"key":"2021042506155833800_R40","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1016\/S0149-7634(05)80183-6","article-title":"Metabolic fuels and reproduction in female mammals","volume":"16","author":"Wade","year":"1992","journal-title":"Neurosci. Biobehav. Rev."},{"key":"2021042506155833800_R41","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1016\/j.physbeh.2007.04.015","article-title":"Prolactin and the hyperphagia of lactation","volume":"91","author":"Woodside","year":"2007","journal-title":"Physiol. Behav."},{"key":"2021042506155833800_R42","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1006\/hbeh.2000.1598","article-title":"Changes in leptin levels during lactation: implications for lactational hyperphagia and anovulation","volume":"37","author":"Woodside","year":"2000","journal-title":"Horm. Behav."},{"key":"2021042506155833800_R43","doi-asserted-by":"crossref","first-page":"4391","DOI":"10.1210\/en.2005-0561","article-title":"Deoxyribonucleic acid microarray analysis of gene expression pattern in the arcuate nucleus\/ventromedial nucleus of hypothalamus during lactation","volume":"146","author":"Xiao","year":"2005","journal-title":"Endocrinology"},{"key":"2021042506155833800_R44","doi-asserted-by":"crossref","first-page":"512","DOI":"10.1242\/jeb.02659","article-title":"Thermogenesis, food intake and serum leptin in cold-exposed lactating Brandt\u2019s voles Lasiopodomys brandtii","volume":"210","author":"Zhang","year":"2007","journal-title":"J. Exp. 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