{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T13:38:50Z","timestamp":1774359530844,"version":"3.50.1"},"reference-count":43,"publisher":"The Company of Biologists","issue":"23","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2011,12,1]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>The anal papillae of freshwater mosquito larvae are important sites of NaCl uptake, thereby acting to offset the dilution of the hemolymph by the dilute habitat. The ion-transport mechanisms in the anal papillae are not well understood. In this study, the scanning ion-selective electrode technique (SIET) was utilized to measure ion fluxes at the anal papillae, and pharmacological inhibitors of ion transport were utilized to identify ion-transport mechanisms. Na+ uptake by the anal papillae was inhibited by bafilomycin and phenamil but not by HMA. Cl\u2013 uptake was inhibited by methazolamide, SITS and DIDS but not by bafilomycin. H+ secretion was inhibited by bafilomycin and methazolamide. Ouabain and bumetanide had no effect on NaCl uptake or H+ secretion. Together, the results suggest that Na+ uptake at the apical membrane occurs through a Na+ channel that is driven by a V-type H+-ATPase and that Cl\u2013 uptake occurs through a Cl\u2013\/HCO3\u2013 exchanger, with carbonic anhydrase providing H+ and HCO3\u2013 to the V-type H+-ATPase and exchanger, respectively.<\/jats:p>","DOI":"10.1242\/jeb.063719","type":"journal-article","created":{"date-parts":[[2011,11,9]],"date-time":"2011-11-09T18:19:55Z","timestamp":1320862795000},"page":"3992-3999","source":"Crossref","is-referenced-by-count":34,"title":["Pharmacological characterisation of apical Na+ and Cl\u2013 transport mechanisms of the anal papillae in the larval mosquito<i>Aedes aegypti<\/i>"],"prefix":"10.1242","volume":"214","author":[{"given":"Olimpia Del","family":"Duca","sequence":"first","affiliation":[{"name":"Department of Biology, York University, 4700 Keele Street, Toronto, ON, Canada, M3J 1P3"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Azadeh","family":"Nasirian","sequence":"additional","affiliation":[{"name":"Department of Biology, York University, 4700 Keele Street, Toronto, ON, Canada, M3J 1P3"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vladimir","family":"Galperin","sequence":"additional","affiliation":[{"name":"Department of Biology, York University, 4700 Keele Street, Toronto, ON, Canada, M3J 1P3"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew","family":"Donini","sequence":"additional","affiliation":[{"name":"Department of Biology, York University, 4700 Keele Street, Toronto, ON, Canada, M3J 1P3"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","reference":[{"key":"2021042506273939400_R1","doi-asserted-by":"crossref","first-page":"3283","DOI":"10.1242\/jeb.033910","article-title":"The involvement of SLC26 anion transporters in chloride uptake in zebrafish (Danio rerio) larvae","volume":"212","author":"Bayaa","year":"2009","journal-title":"J. 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